Vitiosangium sp. GDMCC 1.1324
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4VU12|A0A2T4VU12_9DELT DUF1109 domain-containing protein OS=Vitiosangium sp. GDMCC 1.1324 OX=2138576 GN=DAT35_00735 PE=4 SV=1
MM1 pKa = 7.05 TVCNQGDD8 pKa = 4.09 VPGSTIVEE16 pKa = 4.27 LVLSSDD22 pKa = 3.64 TSILSGWTLPGDD34 pKa = 3.66 VHH36 pKa = 8.04 LDD38 pKa = 3.44 SASTGTLLPQEE49 pKa = 4.71 CQTIPFSLWAPSALAWGGWYY69 pKa = 10.46 LGGLADD75 pKa = 5.01 PSGQQLEE82 pKa = 4.41 LLEE85 pKa = 4.79 SNNGLAGDD93 pKa = 4.35 LVSVGRR99 pKa = 11.84 LADD102 pKa = 3.69 LVVQSVSGPASTRR115 pKa = 11.84 QDD117 pKa = 3.36 APLEE121 pKa = 4.15 ASVTVCNQGYY131 pKa = 9.85 LSSSPTRR138 pKa = 11.84 VEE140 pKa = 4.79 LYY142 pKa = 10.44 LSQDD146 pKa = 3.22 EE147 pKa = 4.82 VIIPSGPSPGSDD159 pKa = 2.71 VFLGRR164 pKa = 11.84 VDD166 pKa = 3.72 VGYY169 pKa = 10.8 LNSDD173 pKa = 3.53 EE174 pKa = 4.66 CTTLPVSSLFQPVGTWRR191 pKa = 11.84 LGAFVNPRR199 pKa = 11.84 GSVQEE204 pKa = 4.37 STWSNNGRR212 pKa = 11.84 AGNTVVVEE220 pKa = 4.19 PP221 pKa = 4.68
Molecular weight: 23.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.973
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A2T4VAN7|A0A2T4VAN7_9DELT Uncharacterized protein OS=Vitiosangium sp. GDMCC 1.1324 OX=2138576 GN=DAT35_37960 PE=3 SV=1
MM1 pKa = 7.23 SFAWHH6 pKa = 6.57 SGRR9 pKa = 11.84 PTRR12 pKa = 11.84 LATRR16 pKa = 11.84 RR17 pKa = 11.84 PARR20 pKa = 11.84 LLRR23 pKa = 11.84 PARR26 pKa = 11.84 LLRR29 pKa = 11.84 PARR32 pKa = 11.84 LLRR35 pKa = 11.84 PARR38 pKa = 11.84 LLRR41 pKa = 11.84 PARR44 pKa = 11.84 LARR47 pKa = 11.84 LARR50 pKa = 11.84 GSSARR55 pKa = 11.84 PARR58 pKa = 11.84 LSPTRR63 pKa = 11.84 GPCCGRR69 pKa = 11.84 GRR71 pKa = 11.84 RR72 pKa = 11.84 FRR74 pKa = 4.57
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.276
Rodwell 12.296
Grimsley 12.837
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.018
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11115
0
11115
4118807
24
4569
370.6
40.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.364 ± 0.035
0.977 ± 0.01
4.89 ± 0.016
6.689 ± 0.023
3.375 ± 0.014
8.602 ± 0.024
2.154 ± 0.012
3.095 ± 0.016
2.989 ± 0.02
11.135 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.944 ± 0.01
2.236 ± 0.014
6.141 ± 0.024
3.473 ± 0.015
7.945 ± 0.028
6.019 ± 0.019
5.415 ± 0.024
7.882 ± 0.019
1.462 ± 0.009
2.213 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here