Fusarium graminearum dsRNA mycovirus-1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5GHF6|Q5GHF6_9VIRU RNA-directed RNA polymerase OS=Fusarium graminearum dsRNA mycovirus-1 OX=194397 PE=4 SV=2
MM1 pKa = 7.55DD2 pKa = 4.21TKK4 pKa = 10.64DD5 pKa = 3.4TLSRR9 pKa = 11.84AQEE12 pKa = 3.73VHH14 pKa = 5.94TNSGISLFIADD25 pKa = 5.19FNLKK29 pKa = 10.05DD30 pKa = 3.78LQALLGNTPRR40 pKa = 11.84GADD43 pKa = 3.63GEE45 pKa = 4.48SSAACLPEE53 pKa = 3.91TSSNVSLDD61 pKa = 3.83RR62 pKa = 11.84QQHH65 pKa = 6.47AIASSAVGKK74 pKa = 8.22YY75 pKa = 8.98TGVSEE80 pKa = 4.51PDD82 pKa = 3.01LTSRR86 pKa = 11.84LARR89 pKa = 11.84LPEE92 pKa = 3.87WFLPRR97 pKa = 11.84IAPAVSSGSKK107 pKa = 10.53ALLEE111 pKa = 3.95ASRR114 pKa = 11.84ASFRR118 pKa = 11.84EE119 pKa = 3.55DD120 pKa = 3.15RR121 pKa = 11.84EE122 pKa = 4.01RR123 pKa = 11.84AKK125 pKa = 10.87AADD128 pKa = 3.64DD129 pKa = 4.21CGPLLGDD136 pKa = 3.63IQRR139 pKa = 11.84VLSFLSYY146 pKa = 8.71LWKK149 pKa = 10.4RR150 pKa = 11.84ALHH153 pKa = 5.88PP154 pKa = 4.28

Molecular weight: 16.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GHF5|Q5GHF5_9VIRU ORF 2 OS=Fusarium graminearum dsRNA mycovirus-1 OX=194397 PE=4 SV=2
MM1 pKa = 7.83KK2 pKa = 10.12ILLIQTRR9 pKa = 11.84PEE11 pKa = 4.01DD12 pKa = 3.77LACEE16 pKa = 4.23SPRR19 pKa = 11.84ASLTHH24 pKa = 6.86DD25 pKa = 4.15EE26 pKa = 5.08RR27 pKa = 11.84NQQQPRR33 pKa = 11.84TGPTICSFRR42 pKa = 11.84THH44 pKa = 6.14RR45 pKa = 11.84RR46 pKa = 11.84IFPKK50 pKa = 10.4EE51 pKa = 3.83GNN53 pKa = 3.46

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2186

53

1550

546.5

61.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.469 ± 1.582

1.098 ± 0.257

5.398 ± 0.425

5.261 ± 1.699

5.169 ± 0.95

5.352 ± 1.076

2.333 ± 0.29

4.483 ± 0.81

4.986 ± 1.503

11.208 ± 0.474

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.738 ± 0.274

3.111 ± 0.117

4.62 ± 0.492

3.431 ± 0.506

6.587 ± 0.877

8.143 ± 0.92

5.901 ± 0.369

6.587 ± 1.6

2.333 ± 0.68

2.79 ± 0.604
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski