Muriicola sp. MMS17-SY002
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2593 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411EC01|A0A411EC01_9FLAO Carbohydrate kinase OS=Muriicola sp. MMS17-SY002 OX=2507538 GN=EQY75_11330 PE=4 SV=1
MM1 pKa = 7.29 IRR3 pKa = 11.84 KK4 pKa = 8.94 LLVLCLLAGSFSYY17 pKa = 11.0 AQFNQNAPWLAGPDD31 pKa = 3.64 EE32 pKa = 4.9 SEE34 pKa = 4.36 LRR36 pKa = 11.84 TPSTVQPLSIFEE48 pKa = 4.12 ISASAEE54 pKa = 4.08 AYY56 pKa = 7.68 WKK58 pKa = 11.16 NKK60 pKa = 10.47 DD61 pKa = 3.41 KK62 pKa = 10.86 DD63 pKa = 3.98 AKK65 pKa = 10.54 GSGYY69 pKa = 10.67 KK70 pKa = 9.83 PFKK73 pKa = 9.96 RR74 pKa = 11.84 WEE76 pKa = 4.45 DD77 pKa = 3.55 YY78 pKa = 10.03 WMHH81 pKa = 6.67 FVDD84 pKa = 3.89 QQGYY88 pKa = 9.67 LPTPKK93 pKa = 10.06 EE94 pKa = 3.83 LWEE97 pKa = 4.0 TWEE100 pKa = 4.12 NKK102 pKa = 7.07 QNRR105 pKa = 11.84 IGMTTNPVSAWSSVGPEE122 pKa = 3.98 TVGVFSGRR130 pKa = 11.84 LPGTGRR136 pKa = 11.84 TNAIEE141 pKa = 4.08 VDD143 pKa = 3.83 PNDD146 pKa = 3.83 PNTWYY151 pKa = 10.7 VGAPAGGIWKK161 pKa = 7.23 TTDD164 pKa = 4.52 AGNTWTNLFDD174 pKa = 5.48 DD175 pKa = 5.51 FPQIGVSSIAIDD187 pKa = 4.03 PNDD190 pKa = 3.74 SNIIYY195 pKa = 10.22 IATGDD200 pKa = 3.81 DD201 pKa = 3.75 DD202 pKa = 5.65 AADD205 pKa = 3.96 SYY207 pKa = 11.81 SVGVFKK213 pKa = 11.17 SLDD216 pKa = 3.43 AGATWQEE223 pKa = 4.04 TGLNPSTSNFSTLMTVILIDD243 pKa = 3.75 PTDD246 pKa = 4.3 SNVLWVATNTGLYY259 pKa = 10.14 KK260 pKa = 10.81 SIDD263 pKa = 3.85 AGDD266 pKa = 3.35 TWNIKK271 pKa = 9.11 QGGYY275 pKa = 9.75 IADD278 pKa = 4.41 LKK280 pKa = 10.89 LKK282 pKa = 10.34 PGDD285 pKa = 3.8 ANTVYY290 pKa = 10.74 AIVGRR295 pKa = 11.84 YY296 pKa = 9.38 LGGSGNQVTFYY307 pKa = 11.0 KK308 pKa = 9.99 STDD311 pKa = 3.3 GGDD314 pKa = 3.74 TPFVALDD321 pKa = 4.14 DD322 pKa = 4.72 PLLPTSSGRR331 pKa = 11.84 ALLGVTPADD340 pKa = 3.64 PEE342 pKa = 4.03 VLYY345 pKa = 10.56 ILTANTGSSNFTFQGLYY362 pKa = 10.45 KK363 pKa = 9.87 STDD366 pKa = 3.06 SGEE369 pKa = 4.42 TFTEE373 pKa = 4.5 SPNTTNIMEE382 pKa = 4.45 SSQAWFDD389 pKa = 3.57 LAFEE393 pKa = 4.23 VSPTNANEE401 pKa = 4.53 LYY403 pKa = 9.85 MGCLNIWKK411 pKa = 9.71 SVNGGNSFTRR421 pKa = 11.84 LNQWFTNNAAYY432 pKa = 8.56 THH434 pKa = 6.91 ADD436 pKa = 2.43 IHH438 pKa = 5.86 TIKK441 pKa = 10.41 IFNDD445 pKa = 3.13 QVFACTDD452 pKa = 3.16 GGIYY456 pKa = 10.35 RR457 pKa = 11.84 STNGGGSFTDD467 pKa = 3.92 YY468 pKa = 10.18 TSGMAVGQFYY478 pKa = 10.65 RR479 pKa = 11.84 LSVSPEE485 pKa = 3.79 NSSKK489 pKa = 10.58 MIGGLQDD496 pKa = 3.0 NGGQILQNGVWNNYY510 pKa = 9.28 HH511 pKa = 6.7 GGDD514 pKa = 4.03 GMDD517 pKa = 4.62 NVIDD521 pKa = 4.25 PNNDD525 pKa = 2.67 NLVYY529 pKa = 10.78 GFTQFGGSLNISSNSGQSIGFVGPPRR555 pKa = 11.84 DD556 pKa = 3.89 DD557 pKa = 3.57 QNNTIQGNWITPLAISSTGEE577 pKa = 3.99 VYY579 pKa = 10.83 SGFDD583 pKa = 3.05 AVYY586 pKa = 10.56 KK587 pKa = 10.5 LVGSAWEE594 pKa = 3.9 KK595 pKa = 9.28 WSNDD599 pKa = 3.17 FGDD602 pKa = 4.37 GNIDD606 pKa = 4.65 DD607 pKa = 5.07 IEE609 pKa = 4.29 VDD611 pKa = 3.46 PTDD614 pKa = 4.02 PNVIYY619 pKa = 10.19 AAEE622 pKa = 4.15 GNIIYY627 pKa = 10.25 RR628 pKa = 11.84 SEE630 pKa = 4.7 DD631 pKa = 2.81 GGQNFTTFYY640 pKa = 10.78 LADD643 pKa = 4.11 ADD645 pKa = 3.86 ISDD648 pKa = 3.78 MTVNSNDD655 pKa = 2.98 GSFVYY660 pKa = 10.61 YY661 pKa = 8.6 VTSRR665 pKa = 11.84 RR666 pKa = 11.84 VGISQANQPTQRR678 pKa = 11.84 EE679 pKa = 4.35 IYY681 pKa = 9.57 KK682 pKa = 10.35 IPVNEE687 pKa = 4.05 NGEE690 pKa = 4.17 AGLEE694 pKa = 3.82 VDD696 pKa = 3.09 ITYY699 pKa = 10.09 NIPADD704 pKa = 3.37 QAFFSIVHH712 pKa = 5.81 QGRR715 pKa = 11.84 HH716 pKa = 5.11 TDD718 pKa = 3.01 NPLYY722 pKa = 10.74 VGTSLGVYY730 pKa = 8.72 RR731 pKa = 11.84 TDD733 pKa = 3.46 DD734 pKa = 3.62 TLSEE738 pKa = 4.05 WEE740 pKa = 5.29 DD741 pKa = 4.03 YY742 pKa = 9.28 FTDD745 pKa = 4.14 LPSVAVSDD753 pKa = 5.01 LDD755 pKa = 3.78 ISLDD759 pKa = 3.77 DD760 pKa = 4.22 EE761 pKa = 5.83 LITASTYY768 pKa = 10.82 GRR770 pKa = 11.84 GVWQSPIPIQVPDD783 pKa = 3.41 NDD785 pKa = 3.46 VRR787 pKa = 11.84 LVSLSPANDD796 pKa = 3.18 LVLCGEE802 pKa = 5.28 IIPEE806 pKa = 3.55 IVVEE810 pKa = 4.15 NNGLNPITSVDD821 pKa = 3.15 VSYY824 pKa = 10.5 TIDD827 pKa = 3.67 GGNPQNFNAPVNLNSEE843 pKa = 4.29 EE844 pKa = 4.27 TTTISLPSLNLTTIGQYY861 pKa = 8.14 TLQVTVSITGDD872 pKa = 2.95 AFADD876 pKa = 3.64 NNEE879 pKa = 3.91 ISHH882 pKa = 6.11 SFFVNGFGVGDD893 pKa = 4.42 EE894 pKa = 4.33 INLFEE899 pKa = 4.54 STADD903 pKa = 3.67 NLNTYY908 pKa = 10.16 NDD910 pKa = 3.92 GGGAPLWEE918 pKa = 4.24 RR919 pKa = 11.84 GVPEE923 pKa = 4.92 GSLLNQARR931 pKa = 11.84 SGTQVYY937 pKa = 7.56 GTNLDD942 pKa = 3.76 GNHH945 pKa = 7.38 PDD947 pKa = 3.06 GTIAYY952 pKa = 8.17 LISDD956 pKa = 4.79 CYY958 pKa = 10.36 EE959 pKa = 3.99 LSSILAPVLKK969 pKa = 10.38 FQMAYY974 pKa = 10.47 DD975 pKa = 3.93 LEE977 pKa = 4.68 INFDD981 pKa = 3.48 IVYY984 pKa = 10.24 VEE986 pKa = 4.17 YY987 pKa = 9.99 STDD990 pKa = 5.27 DD991 pKa = 3.52 GANWQVLGSVNSQPNWYY1008 pKa = 10.43 NSDD1011 pKa = 3.52 RR1012 pKa = 11.84 TNEE1015 pKa = 4.0 SSGADD1020 pKa = 4.09 DD1021 pKa = 5.58 DD1022 pKa = 4.96 CQNCPGAQWTGTEE1035 pKa = 3.98 ATLTEE1040 pKa = 4.01 YY1041 pKa = 10.78 AYY1043 pKa = 10.83 DD1044 pKa = 4.14 FTANAALGEE1053 pKa = 4.12 TDD1055 pKa = 4.03 LTNEE1059 pKa = 3.73 NNVIFRR1065 pKa = 11.84 IVFHH1069 pKa = 7.1 SDD1071 pKa = 2.71 PSVNQEE1077 pKa = 3.51 GAIIDD1082 pKa = 3.97 DD1083 pKa = 3.99 LVVEE1087 pKa = 5.15 GFQDD1091 pKa = 5.88 DD1092 pKa = 5.55 DD1093 pKa = 6.19 DD1094 pKa = 6.24 DD1095 pKa = 6.88 DD1096 pKa = 5.82 NDD1098 pKa = 3.99 GVLDD1102 pKa = 4.1 VDD1104 pKa = 4.99 DD1105 pKa = 4.74 NCPLVGNADD1114 pKa = 3.74 QLDD1117 pKa = 3.79 TDD1119 pKa = 4.44 GDD1121 pKa = 4.46 GEE1123 pKa = 4.81 GDD1125 pKa = 4.02 ACDD1128 pKa = 4.15 TDD1130 pKa = 4.59 DD1131 pKa = 6.15 DD1132 pKa = 5.01 NDD1134 pKa = 5.23 GIDD1137 pKa = 5.93 DD1138 pKa = 4.56 IDD1140 pKa = 4.84 DD1141 pKa = 4.2 NCPLIANADD1150 pKa = 3.69 QADD1153 pKa = 3.64 ADD1155 pKa = 4.1 GDD1157 pKa = 4.53 GIGDD1161 pKa = 3.83 VCDD1164 pKa = 5.37 DD1165 pKa = 4.52 DD1166 pKa = 6.5 ADD1168 pKa = 4.07 NDD1170 pKa = 4.37 GVPNAQDD1177 pKa = 3.73 LCDD1180 pKa = 3.85 NTPPGAVVDD1189 pKa = 4.05 VTGCEE1194 pKa = 4.01 IFTLPSNNFSLLAVGEE1210 pKa = 4.62 TCTSNNDD1217 pKa = 2.81 GSIVVNATEE1226 pKa = 4.16 ALNYY1230 pKa = 8.63 TAILSGGGLNQSQSFSDD1247 pKa = 3.26 TFTFEE1252 pKa = 4.06 NLASGSYY1259 pKa = 9.78 DD1260 pKa = 3.13 LCITVEE1266 pKa = 4.14 GQPGYY1271 pKa = 8.65 EE1272 pKa = 3.81 VCYY1275 pKa = 9.94 SVSISEE1281 pKa = 4.5 PEE1283 pKa = 3.98 DD1284 pKa = 3.64 LSVSSKK1290 pKa = 10.35 IDD1292 pKa = 3.3 SFDD1295 pKa = 3.6 NKK1297 pKa = 9.1 VTLDD1301 pKa = 3.52 LSGGKK1306 pKa = 9.8 SYY1308 pKa = 10.35 TINLNGEE1315 pKa = 3.89 IFRR1318 pKa = 11.84 TSEE1321 pKa = 4.17 RR1322 pKa = 11.84 EE1323 pKa = 3.37 ITLPLSQVEE1332 pKa = 4.16 NTLTVRR1338 pKa = 11.84 TDD1340 pKa = 3.26 KK1341 pKa = 11.08 DD1342 pKa = 3.7 CQGVYY1347 pKa = 10.44 SEE1349 pKa = 5.09 TIMLSSEE1356 pKa = 3.75 LLIYY1360 pKa = 8.91 PNPIASGDD1368 pKa = 3.73 LNIYY1372 pKa = 9.82 LGNNSSSQVEE1382 pKa = 4.31 VALFDD1387 pKa = 4.64 LNGRR1391 pKa = 11.84 TVFRR1395 pKa = 11.84 KK1396 pKa = 9.66 EE1397 pKa = 3.57 YY1398 pKa = 9.94 NVQNNEE1404 pKa = 3.29 IKK1406 pKa = 10.88 FNVDD1410 pKa = 2.92 ALSKK1414 pKa = 10.4 GIYY1417 pKa = 8.29 MLNIKK1422 pKa = 9.21 TSSSLMNYY1430 pKa = 9.94 KK1431 pKa = 10.22 IIRR1434 pKa = 11.84 KK1435 pKa = 9.01
Molecular weight: 155.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.706
IPC_protein 3.757
Toseland 3.528
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.884
Patrickios 0.858
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|A0A411ECB5|A0A411ECB5_9FLAO Class I SAM-dependent methyltransferase OS=Muriicola sp. MMS17-SY002 OX=2507538 GN=EQY75_13070 PE=4 SV=1
MM1 pKa = 7.87 PKK3 pKa = 10.35 NNSSRR8 pKa = 11.84 SWIDD12 pKa = 2.81 IVTEE16 pKa = 3.86 LVEE19 pKa = 5.08 LFFKK23 pKa = 10.88 SSSRR27 pKa = 11.84 SKK29 pKa = 10.72 RR30 pKa = 11.84 GRR32 pKa = 11.84 TWHH35 pKa = 4.78 QHH37 pKa = 3.25 VVPYY41 pKa = 10.06 EE42 pKa = 4.0 NAWAVRR48 pKa = 11.84 RR49 pKa = 11.84 EE50 pKa = 4.02 GNKK53 pKa = 10.25 RR54 pKa = 11.84 ITSKK58 pKa = 10.32 HH59 pKa = 5.07 RR60 pKa = 11.84 RR61 pKa = 11.84 QDD63 pKa = 3.31 TAINKK68 pKa = 9.21 AKK70 pKa = 10.53 RR71 pKa = 11.84 LARR74 pKa = 11.84 KK75 pKa = 9.28 YY76 pKa = 9.39 KK77 pKa = 10.16 ADD79 pKa = 3.92 VIIHH83 pKa = 6.54 RR84 pKa = 11.84 EE85 pKa = 3.94 DD86 pKa = 3.32 GSIRR90 pKa = 11.84 DD91 pKa = 4.16 RR92 pKa = 11.84 INYY95 pKa = 8.03 DD96 pKa = 2.82
Molecular weight: 11.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.809
IPC_protein 10.701
Toseland 10.979
ProMoST 10.745
Dawson 11.038
Bjellqvist 10.76
Wikipedia 11.272
Rodwell 11.242
Grimsley 11.067
Solomon 11.213
Lehninger 11.169
Nozaki 10.95
DTASelect 10.76
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.965
Patrickios 10.979
IPC_peptide 11.228
IPC2_peptide 9.589
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2593
0
2593
868456
38
4472
334.9
37.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.787 ± 0.042
0.726 ± 0.02
5.685 ± 0.062
7.013 ± 0.047
5.054 ± 0.042
6.895 ± 0.056
1.79 ± 0.026
7.352 ± 0.047
6.922 ± 0.07
9.775 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.366 ± 0.026
5.184 ± 0.042
3.743 ± 0.033
3.39 ± 0.032
4.117 ± 0.038
6.452 ± 0.037
5.377 ± 0.057
6.32 ± 0.038
1.131 ± 0.019
3.922 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here