Macrococcus sp. DPC7161

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Macrococcus; unclassified Macrococcus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1M1R4|A0A4V1M1R4_9STAP RNA-binding protein S1 OS=Macrococcus sp. DPC7161 OX=2507060 GN=ER639_11605 PE=4 SV=1
LL1 pKa = 7.59NYY3 pKa = 10.41ASSITSAVEE12 pKa = 3.82RR13 pKa = 11.84VDD15 pKa = 4.39GNPNYY20 pKa = 10.15PIDD23 pKa = 3.95YY24 pKa = 8.57TQVFYY29 pKa = 10.71INPLNKK35 pKa = 9.65TMPATSLEE43 pKa = 4.22VVPYY47 pKa = 10.08HH48 pKa = 6.52PSYY51 pKa = 10.83PNSLAQLDD59 pKa = 3.91PSVTNFHH66 pKa = 6.73IYY68 pKa = 10.42EE69 pKa = 4.18YY70 pKa = 10.77TDD72 pKa = 3.45GSLLNDD78 pKa = 4.04SFSNDD83 pKa = 2.89YY84 pKa = 11.2SLAIDD89 pKa = 3.57VTSQYY94 pKa = 11.34SPIWNEE100 pKa = 3.86DD101 pKa = 3.05GTVTFNFGDD110 pKa = 3.31ITKK113 pKa = 8.85TYY115 pKa = 9.73VVVVDD120 pKa = 4.47SVTKK124 pKa = 10.32VDD126 pKa = 4.04PSNKK130 pKa = 9.26ILVQSGLVSADD141 pKa = 3.49TTQGTHH147 pKa = 4.63VTRR150 pKa = 11.84STANLTQAFSGVSIGSPTFKK170 pKa = 10.49LGNFVWNDD178 pKa = 3.22LNKK181 pKa = 10.76NGIQEE186 pKa = 3.86IGEE189 pKa = 4.07NGVANVTVTITNSNGTVVGTTQTDD213 pKa = 3.62VNGGYY218 pKa = 10.57LFQNLPEE225 pKa = 4.15GTYY228 pKa = 9.17TVEE231 pKa = 4.61FSNIPTGFVVSPSNSGTDD249 pKa = 3.32PSLDD253 pKa = 3.76SNGLISTVTIANGDD267 pKa = 3.89NLTADD272 pKa = 4.08LGIYY276 pKa = 9.38EE277 pKa = 4.98PIYY280 pKa = 10.56RR281 pKa = 11.84IGDD284 pKa = 4.05YY285 pKa = 10.88VWLDD289 pKa = 3.31TDD291 pKa = 3.77GDD293 pKa = 4.67GIQGTSVDD301 pKa = 3.72EE302 pKa = 4.61APFEE306 pKa = 4.26GAQVILYY313 pKa = 9.91DD314 pKa = 3.83EE315 pKa = 4.94NGNKK319 pKa = 9.33IAEE322 pKa = 4.38TLTDD326 pKa = 3.05NRR328 pKa = 11.84GYY330 pKa = 11.44YY331 pKa = 9.78EE332 pKa = 5.51FNNLDD337 pKa = 3.29NGTYY341 pKa = 8.86QVEE344 pKa = 5.28FINPNPDD351 pKa = 2.59MLAFTKK357 pKa = 10.65QNAGADD363 pKa = 3.81NVDD366 pKa = 3.84SDD368 pKa = 5.08VSDD371 pKa = 3.55ANNRR375 pKa = 11.84VTVTINNADD384 pKa = 3.55NMTIDD389 pKa = 4.34AGLQQVYY396 pKa = 10.8SIGDD400 pKa = 3.98KK401 pKa = 10.8VWLDD405 pKa = 3.57TNGDD409 pKa = 4.07DD410 pKa = 3.78IQNGGTEE417 pKa = 3.95PGIAGVQVQLVDD429 pKa = 3.45KK430 pKa = 8.13TTGTVIQTQTTDD442 pKa = 2.99VNGDD446 pKa = 3.61YY447 pKa = 10.87LFTGLKK453 pKa = 9.59PGDD456 pKa = 3.65YY457 pKa = 8.97TVVFGTPAQVTNPDD471 pKa = 3.47GTTTNYY477 pKa = 10.51TEE479 pKa = 4.08VV480 pKa = 3.25

Molecular weight: 51.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1B3R0|A0A4Q1B3R0_9STAP Uncharacterized protein OS=Macrococcus sp. DPC7161 OX=2507060 GN=ER639_05715 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.91VHH17 pKa = 5.68GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.58VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.94GRR40 pKa = 11.84KK41 pKa = 8.67VLSAA45 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2341

0

2341

690768

27

3845

295.1

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.154 ± 0.057

0.699 ± 0.015

5.652 ± 0.069

6.83 ± 0.07

4.6 ± 0.047

6.153 ± 0.056

2.383 ± 0.034

8.633 ± 0.065

7.568 ± 0.065

9.336 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.968 ± 0.029

5.205 ± 0.045

3.289 ± 0.053

3.841 ± 0.041

3.314 ± 0.045

5.907 ± 0.098

5.85 ± 0.123

6.928 ± 0.069

0.725 ± 0.017

3.964 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski