Amino acid dipepetide frequency for Macrococcus sp. DPC7161

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.093AlaAla: 4.093 ± 0.113
0.565AlaCys: 0.565 ± 0.033
2.868AlaAsp: 2.868 ± 0.077
3.28AlaGlu: 3.28 ± 0.086
2.998AlaPhe: 2.998 ± 0.077
3.968AlaGly: 3.968 ± 0.112
1.396AlaHis: 1.396 ± 0.051
5.717AlaIle: 5.717 ± 0.12
4.475AlaLys: 4.475 ± 0.095
6.813AlaLeu: 6.813 ± 0.117
2.015AlaMet: 2.015 ± 0.056
2.696AlaAsn: 2.696 ± 0.063
1.973AlaPro: 1.973 ± 0.081
2.119AlaGln: 2.119 ± 0.056
2.021AlaArg: 2.021 ± 0.054
3.832AlaSer: 3.832 ± 0.074
3.551AlaThr: 3.551 ± 0.109
4.453AlaVal: 4.453 ± 0.095
0.465AlaTrp: 0.465 ± 0.028
2.245AlaTyr: 2.245 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.366CysAla: 0.366 ± 0.022
0.096CysCys: 0.096 ± 0.012
0.442CysAsp: 0.442 ± 0.04
0.401CysGlu: 0.401 ± 0.023
0.358CysPhe: 0.358 ± 0.024
0.634CysGly: 0.634 ± 0.036
0.195CysHis: 0.195 ± 0.018
0.689CysIle: 0.689 ± 0.029
0.359CysLys: 0.359 ± 0.024
0.667CysLeu: 0.667 ± 0.032
0.178CysMet: 0.178 ± 0.015
0.345CysAsn: 0.345 ± 0.022
0.282CysPro: 0.282 ± 0.025
0.219CysGln: 0.219 ± 0.019
0.201CysArg: 0.201 ± 0.016
0.413CysSer: 0.413 ± 0.026
0.398CysThr: 0.398 ± 0.026
0.424CysVal: 0.424 ± 0.026
0.039CysTrp: 0.039 ± 0.007
0.288CysTyr: 0.288 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.907AspAla: 3.907 ± 0.097
0.398AspCys: 0.398 ± 0.039
2.903AspAsp: 2.903 ± 0.072
4.701AspGlu: 4.701 ± 0.103
2.672AspPhe: 2.672 ± 0.066
3.375AspGly: 3.375 ± 0.099
1.223AspHis: 1.223 ± 0.042
4.909AspIle: 4.909 ± 0.09
4.149AspLys: 4.149 ± 0.081
5.168AspLeu: 5.168 ± 0.09
1.503AspMet: 1.503 ± 0.046
2.299AspAsn: 2.299 ± 0.062
1.753AspPro: 1.753 ± 0.128
2.096AspGln: 2.096 ± 0.051
1.794AspArg: 1.794 ± 0.05
3.144AspSer: 3.144 ± 0.433
3.017AspThr: 3.017 ± 0.115
4.314AspVal: 4.314 ± 0.111
0.43AspTrp: 0.43 ± 0.028
2.764AspTyr: 2.764 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
5.026GluAla: 5.026 ± 0.094
0.345GluCys: 0.345 ± 0.023
3.603GluAsp: 3.603 ± 0.083
4.701GluGlu: 4.701 ± 0.099
2.216GluPhe: 2.216 ± 0.06
3.529GluGly: 3.529 ± 0.083
1.647GluHis: 1.647 ± 0.053
5.782GluIle: 5.782 ± 0.123
5.905GluLys: 5.905 ± 0.111
5.704GluLeu: 5.704 ± 0.113
2.445GluMet: 2.445 ± 0.062
3.654GluAsn: 3.654 ± 0.083
1.834GluPro: 1.834 ± 0.159
2.972GluGln: 2.972 ± 0.075
3.037GluArg: 3.037 ± 0.085
3.938GluSer: 3.938 ± 0.188
4.168GluThr: 4.168 ± 0.103
4.932GluVal: 4.932 ± 0.104
0.594GluTrp: 0.594 ± 0.029
2.263GluTyr: 2.263 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.422PheAla: 2.422 ± 0.066
0.308PheCys: 0.308 ± 0.021
2.917PheAsp: 2.917 ± 0.07
3.418PheGlu: 3.418 ± 0.077
2.09PhePhe: 2.09 ± 0.073
3.27PheGly: 3.27 ± 0.086
0.958PheHis: 0.958 ± 0.038
4.554PheIle: 4.554 ± 0.128
3.574PheLys: 3.574 ± 0.078
3.983PheLeu: 3.983 ± 0.113
1.265PheMet: 1.265 ± 0.041
2.836PheAsn: 2.836 ± 0.059
1.206PhePro: 1.206 ± 0.036
1.438PheGln: 1.438 ± 0.045
1.196PheArg: 1.196 ± 0.043
2.701PheSer: 2.701 ± 0.07
2.478PheThr: 2.478 ± 0.065
3.189PheVal: 3.189 ± 0.069
0.343PheTrp: 0.343 ± 0.023
1.854PheTyr: 1.854 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
4.029GlyAla: 4.029 ± 0.094
0.498GlyCys: 0.498 ± 0.027
3.182GlyAsp: 3.182 ± 0.081
3.644GlyGlu: 3.644 ± 0.083
3.14GlyPhe: 3.14 ± 0.061
3.965GlyGly: 3.965 ± 0.08
1.449GlyHis: 1.449 ± 0.044
5.731GlyIle: 5.731 ± 0.103
4.573GlyLys: 4.573 ± 0.083
5.814GlyLeu: 5.814 ± 0.126
1.892GlyMet: 1.892 ± 0.055
2.593GlyAsn: 2.593 ± 0.079
1.548GlyPro: 1.548 ± 0.052
2.125GlyGln: 2.125 ± 0.058
2.064GlyArg: 2.064 ± 0.059
3.597GlySer: 3.597 ± 0.229
3.793GlyThr: 3.793 ± 0.135
4.657GlyVal: 4.657 ± 0.111
0.465GlyTrp: 0.465 ± 0.032
2.768GlyTyr: 2.768 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.339HisAla: 1.339 ± 0.045
0.2HisCys: 0.2 ± 0.02
1.346HisAsp: 1.346 ± 0.044
1.6HisGlu: 1.6 ± 0.052
1.303HisPhe: 1.303 ± 0.046
1.365HisGly: 1.365 ± 0.047
0.903HisHis: 0.903 ± 0.038
2.051HisIle: 2.051 ± 0.061
1.322HisLys: 1.322 ± 0.047
2.371HisLeu: 2.371 ± 0.07
0.63HisMet: 0.63 ± 0.03
0.925HisAsn: 0.925 ± 0.034
1.178HisPro: 1.178 ± 0.046
1.287HisGln: 1.287 ± 0.057
0.812HisArg: 0.812 ± 0.031
1.076HisSer: 1.076 ± 0.042
1.177HisThr: 1.177 ± 0.045
1.649HisVal: 1.649 ± 0.047
0.143HisTrp: 0.143 ± 0.014
1.151HisTyr: 1.151 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
5.655IleAla: 5.655 ± 0.101
0.717IleCys: 0.717 ± 0.035
5.495IleAsp: 5.495 ± 0.089
6.769IleGlu: 6.769 ± 0.149
3.663IlePhe: 3.663 ± 0.097
5.838IleGly: 5.838 ± 0.131
2.112IleHis: 2.112 ± 0.069
7.231IleIle: 7.231 ± 0.143
6.348IleLys: 6.348 ± 0.118
7.151IleLeu: 7.151 ± 0.142
2.076IleMet: 2.076 ± 0.061
4.676IleAsn: 4.676 ± 0.096
3.007IlePro: 3.007 ± 0.066
3.542IleGln: 3.542 ± 0.082
2.578IleArg: 2.578 ± 0.07
5.162IleSer: 5.162 ± 0.15
4.873IleThr: 4.873 ± 0.118
5.838IleVal: 5.838 ± 0.086
0.569IleTrp: 0.569 ± 0.033
3.029IleTyr: 3.029 ± 0.068
0.0IleXaa: 0.0 ± 0.0
Lys
4.84LysAla: 4.84 ± 0.106
0.352LysCys: 0.352 ± 0.022
4.721LysAsp: 4.721 ± 0.097
5.902LysGlu: 5.902 ± 0.106
2.623LysPhe: 2.623 ± 0.065
4.172LysGly: 4.172 ± 0.096
1.854LysHis: 1.854 ± 0.047
5.322LysIle: 5.322 ± 0.099
6.122LysLys: 6.122 ± 0.137
6.4LysLeu: 6.4 ± 0.113
2.48LysMet: 2.48 ± 0.061
3.984LysAsn: 3.984 ± 0.093
2.284LysPro: 2.284 ± 0.056
3.508LysGln: 3.508 ± 0.086
3.101LysArg: 3.101 ± 0.074
4.609LysSer: 4.609 ± 0.099
4.26LysThr: 4.26 ± 0.096
5.393LysVal: 5.393 ± 0.107
0.62LysTrp: 0.62 ± 0.028
3.137LysTyr: 3.137 ± 0.075
0.0LysXaa: 0.0 ± 0.0
Leu
5.448LeuAla: 5.448 ± 0.094
0.693LeuCys: 0.693 ± 0.033
4.88LeuAsp: 4.88 ± 0.101
5.805LeuGlu: 5.805 ± 0.11
4.871LeuPhe: 4.871 ± 0.115
5.681LeuGly: 5.681 ± 0.118
1.812LeuHis: 1.812 ± 0.048
8.055LeuIle: 8.055 ± 0.152
7.526LeuLys: 7.526 ± 0.121
9.32LeuLeu: 9.32 ± 0.169
3.0LeuMet: 3.0 ± 0.077
5.815LeuAsn: 5.815 ± 0.121
3.467LeuPro: 3.467 ± 0.081
2.934LeuGln: 2.934 ± 0.067
2.662LeuArg: 2.662 ± 0.063
6.656LeuSer: 6.656 ± 0.128
5.301LeuThr: 5.301 ± 0.117
5.676LeuVal: 5.676 ± 0.138
0.557LeuTrp: 0.557 ± 0.029
3.202LeuTyr: 3.202 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.766MetAla: 1.766 ± 0.053
0.159MetCys: 0.159 ± 0.013
1.594MetAsp: 1.594 ± 0.05
1.517MetGlu: 1.517 ± 0.048
1.235MetPhe: 1.235 ± 0.04
1.539MetGly: 1.539 ± 0.056
0.777MetHis: 0.777 ± 0.039
2.826MetIle: 2.826 ± 0.069
2.677MetLys: 2.677 ± 0.067
2.833MetLeu: 2.833 ± 0.081
1.155MetMet: 1.155 ± 0.048
1.902MetAsn: 1.902 ± 0.051
1.051MetPro: 1.051 ± 0.041
1.12MetGln: 1.12 ± 0.038
1.025MetArg: 1.025 ± 0.033
1.896MetSer: 1.896 ± 0.049
1.833MetThr: 1.833 ± 0.048
1.579MetVal: 1.579 ± 0.048
0.172MetTrp: 0.172 ± 0.014
1.018MetTyr: 1.018 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.386AsnAla: 3.386 ± 0.071
0.294AsnCys: 0.294 ± 0.02
3.15AsnAsp: 3.15 ± 0.083
4.182AsnGlu: 4.182 ± 0.084
1.818AsnPhe: 1.818 ± 0.05
3.324AsnGly: 3.324 ± 0.086
1.524AsnHis: 1.524 ± 0.05
4.525AsnIle: 4.525 ± 0.103
4.103AsnLys: 4.103 ± 0.088
4.016AsnLeu: 4.016 ± 0.084
1.339AsnMet: 1.339 ± 0.047
2.808AsnAsn: 2.808 ± 0.082
2.111AsnPro: 2.111 ± 0.061
2.856AsnGln: 2.856 ± 0.08
1.869AsnArg: 1.869 ± 0.063
2.118AsnSer: 2.118 ± 0.063
2.756AsnThr: 2.756 ± 0.082
3.489AsnVal: 3.489 ± 0.076
0.36AsnTrp: 0.36 ± 0.022
2.022AsnTyr: 2.022 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
1.698ProAla: 1.698 ± 0.115
0.195ProCys: 0.195 ± 0.016
1.804ProAsp: 1.804 ± 0.122
2.557ProGlu: 2.557 ± 0.066
1.804ProPhe: 1.804 ± 0.049
2.057ProGly: 2.057 ± 0.073
0.731ProHis: 0.731 ± 0.034
2.795ProIle: 2.795 ± 0.094
2.329ProLys: 2.329 ± 0.066
3.162ProLeu: 3.162 ± 0.149
0.932ProMet: 0.932 ± 0.047
1.907ProAsn: 1.907 ± 0.051
0.708ProPro: 0.708 ± 0.041
0.942ProGln: 0.942 ± 0.034
0.828ProArg: 0.828 ± 0.035
1.988ProSer: 1.988 ± 0.053
2.135ProThr: 2.135 ± 0.11
2.658ProVal: 2.658 ± 0.114
0.242ProTrp: 0.242 ± 0.018
1.419ProTyr: 1.419 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.228GlnAla: 2.228 ± 0.056
0.21GlnCys: 0.21 ± 0.018
1.865GlnAsp: 1.865 ± 0.058
2.187GlnGlu: 2.187 ± 0.064
2.025GlnPhe: 2.025 ± 0.06
1.828GlnGly: 1.828 ± 0.058
0.929GlnHis: 0.929 ± 0.04
2.972GlnIle: 2.972 ± 0.073
2.879GlnLys: 2.879 ± 0.076
4.398GlnLeu: 4.398 ± 0.097
1.391GlnMet: 1.391 ± 0.05
2.125GlnAsn: 2.125 ± 0.061
1.242GlnPro: 1.242 ± 0.055
1.649GlnGln: 1.649 ± 0.069
1.129GlnArg: 1.129 ± 0.043
2.599GlnSer: 2.599 ± 0.073
2.118GlnThr: 2.118 ± 0.062
2.416GlnVal: 2.416 ± 0.064
0.285GlnTrp: 0.285 ± 0.019
1.933GlnTyr: 1.933 ± 0.063
0.0GlnXaa: 0.0 ± 0.0
Arg
2.021ArgAla: 2.021 ± 0.067
0.193ArgCys: 0.193 ± 0.017
1.883ArgAsp: 1.883 ± 0.052
2.279ArgGlu: 2.279 ± 0.072
1.647ArgPhe: 1.647 ± 0.051
1.947ArgGly: 1.947 ± 0.058
0.786ArgHis: 0.786 ± 0.035
2.819ArgIle: 2.819 ± 0.062
2.562ArgLys: 2.562 ± 0.057
3.24ArgLeu: 3.24 ± 0.08
1.118ArgMet: 1.118 ± 0.038
1.729ArgAsn: 1.729 ± 0.053
1.023ArgPro: 1.023 ± 0.038
1.254ArgGln: 1.254 ± 0.042
1.461ArgArg: 1.461 ± 0.053
1.601ArgSer: 1.601 ± 0.048
1.75ArgThr: 1.75 ± 0.049
2.251ArgVal: 2.251 ± 0.062
0.239ArgTrp: 0.239 ± 0.017
1.339ArgTyr: 1.339 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
3.082SerAla: 3.082 ± 0.069
0.4SerCys: 0.4 ± 0.024
3.17SerAsp: 3.17 ± 0.07
4.132SerGlu: 4.132 ± 0.192
2.911SerPhe: 2.911 ± 0.076
4.14SerGly: 4.14 ± 0.219
1.43SerHis: 1.43 ± 0.048
5.416SerIle: 5.416 ± 0.141
4.44SerLys: 4.44 ± 0.101
5.602SerLeu: 5.602 ± 0.14
1.655SerMet: 1.655 ± 0.051
3.156SerAsn: 3.156 ± 0.084
1.682SerPro: 1.682 ± 0.061
1.956SerGln: 1.956 ± 0.07
1.882SerArg: 1.882 ± 0.055
3.144SerSer: 3.144 ± 0.089
3.679SerThr: 3.679 ± 0.291
4.444SerVal: 4.444 ± 0.33
0.401SerTrp: 0.401 ± 0.029
2.352SerTyr: 2.352 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.389ThrAla: 3.389 ± 0.119
0.391ThrCys: 0.391 ± 0.023
3.639ThrAsp: 3.639 ± 0.384
3.557ThrGlu: 3.557 ± 0.125
2.882ThrPhe: 2.882 ± 0.069
3.871ThrGly: 3.871 ± 0.12
1.433ThrHis: 1.433 ± 0.048
5.026ThrIle: 5.026 ± 0.081
4.025ThrLys: 4.025 ± 0.078
5.66ThrLeu: 5.66 ± 0.109
1.411ThrMet: 1.411 ± 0.047
2.861ThrAsn: 2.861 ± 0.074
2.516ThrPro: 2.516 ± 0.136
1.77ThrGln: 1.77 ± 0.056
1.685ThrArg: 1.685 ± 0.048
3.634ThrSer: 3.634 ± 0.296
3.706ThrThr: 3.706 ± 0.243
4.174ThrVal: 4.174 ± 0.22
0.437ThrTrp: 0.437 ± 0.035
2.436ThrTyr: 2.436 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
4.168ValAla: 4.168 ± 0.099
0.589ValCys: 0.589 ± 0.028
4.165ValAsp: 4.165 ± 0.236
4.456ValGlu: 4.456 ± 0.101
2.971ValPhe: 2.971 ± 0.063
4.25ValGly: 4.25 ± 0.08
1.42ValHis: 1.42 ± 0.045
6.058ValIle: 6.058 ± 0.115
5.219ValLys: 5.219 ± 0.107
6.349ValLeu: 6.349 ± 0.109
2.001ValMet: 2.001 ± 0.062
3.511ValAsn: 3.511 ± 0.071
2.436ValPro: 2.436 ± 0.067
2.27ValGln: 2.27 ± 0.054
2.112ValArg: 2.112 ± 0.059
4.459ValSer: 4.459 ± 0.106
5.042ValThr: 5.042 ± 0.463
4.951ValVal: 4.951 ± 0.101
0.486ValTrp: 0.486 ± 0.029
2.363ValTyr: 2.363 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.388TrpAla: 0.388 ± 0.023
0.068TrpCys: 0.068 ± 0.011
0.332TrpAsp: 0.332 ± 0.021
0.359TrpGlu: 0.359 ± 0.026
0.45TrpPhe: 0.45 ± 0.026
0.411TrpGly: 0.411 ± 0.029
0.203TrpHis: 0.203 ± 0.017
0.731TrpIle: 0.731 ± 0.035
0.394TrpLys: 0.394 ± 0.026
0.876TrpLeu: 0.876 ± 0.034
0.278TrpMet: 0.278 ± 0.023
0.353TrpAsn: 0.353 ± 0.026
0.2TrpPro: 0.2 ± 0.018
0.326TrpGln: 0.326 ± 0.023
0.217TrpArg: 0.217 ± 0.017
0.429TrpSer: 0.429 ± 0.031
0.382TrpThr: 0.382 ± 0.027
0.463TrpVal: 0.463 ± 0.029
0.085TrpTrp: 0.085 ± 0.013
0.308TrpTyr: 0.308 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.292TyrAla: 2.292 ± 0.063
0.324TyrCys: 0.324 ± 0.023
2.559TyrAsp: 2.559 ± 0.066
2.85TyrGlu: 2.85 ± 0.07
2.324TyrPhe: 2.324 ± 0.068
2.36TyrGly: 2.36 ± 0.058
1.042TyrHis: 1.042 ± 0.043
3.12TyrIle: 3.12 ± 0.07
2.703TyrLys: 2.703 ± 0.066
3.836TyrLeu: 3.836 ± 0.084
0.912TyrMet: 0.912 ± 0.036
1.879TyrAsn: 1.879 ± 0.052
1.388TyrPro: 1.388 ± 0.044
2.025TyrGln: 2.025 ± 0.059
1.466TyrArg: 1.466 ± 0.046
2.079TyrSer: 2.079 ± 0.061
2.086TyrThr: 2.086 ± 0.056
2.325TyrVal: 2.325 ± 0.063
0.32TyrTrp: 0.32 ± 0.023
1.744TyrTyr: 1.744 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2341 proteins (690768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski