Pseudomonas phage Waldo5
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G8LJP5|A0A7G8LJP5_9CAUD Uncharacterized protein OS=Pseudomonas phage Waldo5 OX=2762290 PE=4 SV=1
MM1 pKa = 7.37 LKK3 pKa = 10.42 VIQHH7 pKa = 6.93 HH8 pKa = 5.92 IQNPDD13 pKa = 4.59 DD14 pKa = 4.22 IPSIPPASAEE24 pKa = 4.01 YY25 pKa = 10.53 LAVRR29 pKa = 11.84 LNASYY34 pKa = 10.91 LIATGVVDD42 pKa = 4.97 DD43 pKa = 5.2 LRR45 pKa = 11.84 KK46 pKa = 9.42 QGYY49 pKa = 8.57 SEE51 pKa = 5.12 GYY53 pKa = 9.22 IAGFLDD59 pKa = 3.89 GCNAAVEE66 pKa = 4.57 ITDD69 pKa = 5.11 LMQEE73 pKa = 4.06 SQLQKK78 pKa = 11.18 EE79 pKa = 4.56 EE80 pKa = 4.0
Molecular weight: 8.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.279
IPC2_protein 4.444
IPC_protein 4.291
Toseland 4.126
ProMoST 4.342
Dawson 4.266
Bjellqvist 4.495
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 3.121
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.333
Protein with the highest isoelectric point:
>tr|A0A7G8LJN8|A0A7G8LJN8_9CAUD Uncharacterized protein OS=Pseudomonas phage Waldo5 OX=2762290 PE=4 SV=1
MM1 pKa = 7.52 KK2 pKa = 10.07 ALKK5 pKa = 10.78 SFDD8 pKa = 3.28 IVTALILLAGRR19 pKa = 11.84 IAQRR23 pKa = 11.84 RR24 pKa = 11.84 HH25 pKa = 4.24 QKK27 pKa = 10.08 LVARR31 pKa = 11.84 EE32 pKa = 4.23 ASLKK36 pKa = 10.54 AAIAATQRR44 pKa = 11.84 AYY46 pKa = 10.99 QDD48 pKa = 3.43 TVSARR53 pKa = 11.84 VAADD57 pKa = 3.28 YY58 pKa = 9.93 RR59 pKa = 11.84 AQDD62 pKa = 3.31 ITRR65 pKa = 11.84 VKK67 pKa = 10.79
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.955
IPC_protein 10.965
Toseland 11.125
ProMoST 11.169
Dawson 11.169
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.316
Grimsley 11.213
Solomon 11.433
Lehninger 11.374
Nozaki 11.096
DTASelect 10.965
Thurlkill 11.111
EMBOSS 11.55
Sillero 11.125
Patrickios 11.082
IPC_peptide 11.433
IPC2_peptide 10.072
IPC2.peptide.svr19 8.56
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
12666
40
1393
243.6
27.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.64 ± 0.392
0.947 ± 0.174
6.458 ± 0.241
6.695 ± 0.32
3.805 ± 0.198
7.911 ± 0.297
1.99 ± 0.202
5.053 ± 0.241
5.787 ± 0.301
8.108 ± 0.353
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.582 ± 0.164
3.829 ± 0.212
4.003 ± 0.238
4.35 ± 0.342
5.985 ± 0.243
5.803 ± 0.311
5.692 ± 0.29
6.766 ± 0.367
1.476 ± 0.118
3.119 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here