Amino acid dipepetide frequency for Pseudomonas phage Waldo5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.054AlaAla: 11.054 ± 1.511
1.263AlaCys: 1.263 ± 0.378
5.685AlaAsp: 5.685 ± 0.818
6.396AlaGlu: 6.396 ± 0.831
3.316AlaPhe: 3.316 ± 0.673
7.264AlaGly: 7.264 ± 0.86
1.105AlaHis: 1.105 ± 0.22
4.895AlaIle: 4.895 ± 0.632
5.527AlaLys: 5.527 ± 0.687
8.685AlaLeu: 8.685 ± 0.816
3.079AlaMet: 3.079 ± 0.677
3.79AlaAsn: 3.79 ± 0.593
3.158AlaPro: 3.158 ± 0.465
5.132AlaGln: 5.132 ± 0.657
6.159AlaArg: 6.159 ± 0.814
4.895AlaSer: 4.895 ± 0.575
5.448AlaThr: 5.448 ± 0.537
5.29AlaVal: 5.29 ± 0.67
1.342AlaTrp: 1.342 ± 0.35
2.921AlaTyr: 2.921 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
1.026CysAla: 1.026 ± 0.306
0.158CysCys: 0.158 ± 0.107
0.632CysAsp: 0.632 ± 0.316
0.474CysGlu: 0.474 ± 0.151
0.711CysPhe: 0.711 ± 0.21
0.79CysGly: 0.79 ± 0.244
0.237CysHis: 0.237 ± 0.117
0.474CysIle: 0.474 ± 0.189
0.711CysLys: 0.711 ± 0.303
0.79CysLeu: 0.79 ± 0.311
0.237CysMet: 0.237 ± 0.133
0.316CysAsn: 0.316 ± 0.173
0.158CysPro: 0.158 ± 0.12
0.395CysGln: 0.395 ± 0.18
0.711CysArg: 0.711 ± 0.314
0.474CysSer: 0.474 ± 0.22
0.079CysThr: 0.079 ± 0.09
0.79CysVal: 0.79 ± 0.28
0.079CysTrp: 0.079 ± 0.097
0.237CysTyr: 0.237 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
5.527AspAla: 5.527 ± 0.643
0.632AspCys: 0.632 ± 0.29
4.501AspAsp: 4.501 ± 0.695
4.264AspGlu: 4.264 ± 0.612
3.711AspPhe: 3.711 ± 0.492
5.527AspGly: 5.527 ± 0.51
1.421AspHis: 1.421 ± 0.432
3.395AspIle: 3.395 ± 0.606
3.79AspLys: 3.79 ± 0.589
5.527AspLeu: 5.527 ± 0.608
1.974AspMet: 1.974 ± 0.327
2.685AspAsn: 2.685 ± 0.472
3.237AspPro: 3.237 ± 0.818
1.816AspGln: 1.816 ± 0.379
3.158AspArg: 3.158 ± 0.563
2.606AspSer: 2.606 ± 0.503
3.632AspThr: 3.632 ± 0.567
3.869AspVal: 3.869 ± 0.542
1.263AspTrp: 1.263 ± 0.309
1.974AspTyr: 1.974 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
7.817GluAla: 7.817 ± 0.89
0.79GluCys: 0.79 ± 0.254
4.343GluAsp: 4.343 ± 0.394
4.816GluGlu: 4.816 ± 0.749
2.921GluPhe: 2.921 ± 0.401
5.132GluGly: 5.132 ± 0.59
1.263GluHis: 1.263 ± 0.352
2.448GluIle: 2.448 ± 0.414
2.369GluLys: 2.369 ± 0.461
4.895GluLeu: 4.895 ± 0.588
1.816GluMet: 1.816 ± 0.354
1.895GluAsn: 1.895 ± 0.43
1.737GluPro: 1.737 ± 0.282
3.553GluGln: 3.553 ± 0.72
4.58GluArg: 4.58 ± 0.591
4.106GluSer: 4.106 ± 0.569
3.395GluThr: 3.395 ± 0.387
5.053GluVal: 5.053 ± 0.783
1.263GluTrp: 1.263 ± 0.46
2.764GluTyr: 2.764 ± 0.409
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.377
0.632PheCys: 0.632 ± 0.246
2.606PheAsp: 2.606 ± 0.393
2.053PheGlu: 2.053 ± 0.311
1.342PhePhe: 1.342 ± 0.343
2.921PheGly: 2.921 ± 0.487
0.869PheHis: 0.869 ± 0.259
2.053PheIle: 2.053 ± 0.446
1.816PheLys: 1.816 ± 0.337
3.158PheLeu: 3.158 ± 0.436
1.184PheMet: 1.184 ± 0.339
1.895PheAsn: 1.895 ± 0.322
1.658PhePro: 1.658 ± 0.341
1.184PheGln: 1.184 ± 0.408
2.211PheArg: 2.211 ± 0.344
2.527PheSer: 2.527 ± 0.558
2.764PheThr: 2.764 ± 0.508
3.079PheVal: 3.079 ± 0.454
0.553PheTrp: 0.553 ± 0.239
0.474PheTyr: 0.474 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
6.948GlyAla: 6.948 ± 1.168
0.711GlyCys: 0.711 ± 0.225
4.974GlyAsp: 4.974 ± 0.646
5.053GlyGlu: 5.053 ± 0.46
3.0GlyPhe: 3.0 ± 0.416
6.317GlyGly: 6.317 ± 0.742
2.29GlyHis: 2.29 ± 0.442
4.816GlyIle: 4.816 ± 0.72
4.659GlyLys: 4.659 ± 0.562
6.317GlyLeu: 6.317 ± 1.048
1.895GlyMet: 1.895 ± 0.327
2.211GlyAsn: 2.211 ± 0.457
2.29GlyPro: 2.29 ± 0.417
3.553GlyGln: 3.553 ± 0.446
4.343GlyArg: 4.343 ± 0.51
5.527GlySer: 5.527 ± 0.668
4.895GlyThr: 4.895 ± 0.716
4.422GlyVal: 4.422 ± 0.554
1.816GlyTrp: 1.816 ± 0.385
3.079GlyTyr: 3.079 ± 0.479
0.0GlyXaa: 0.0 ± 0.0
His
0.869HisAla: 0.869 ± 0.274
0.316HisCys: 0.316 ± 0.187
1.342HisAsp: 1.342 ± 0.309
1.342HisGlu: 1.342 ± 0.468
0.869HisPhe: 0.869 ± 0.236
1.5HisGly: 1.5 ± 0.277
0.316HisHis: 0.316 ± 0.182
1.737HisIle: 1.737 ± 0.304
1.026HisLys: 1.026 ± 0.29
1.737HisLeu: 1.737 ± 0.323
1.105HisMet: 1.105 ± 0.318
0.553HisAsn: 0.553 ± 0.196
0.79HisPro: 0.79 ± 0.272
0.79HisGln: 0.79 ± 0.266
0.711HisArg: 0.711 ± 0.242
0.947HisSer: 0.947 ± 0.456
1.184HisThr: 1.184 ± 0.342
1.105HisVal: 1.105 ± 0.319
0.79HisTrp: 0.79 ± 0.279
0.869HisTyr: 0.869 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
4.264IleAla: 4.264 ± 0.562
0.553IleCys: 0.553 ± 0.207
3.0IleAsp: 3.0 ± 0.379
3.79IleGlu: 3.79 ± 0.508
1.026IlePhe: 1.026 ± 0.342
3.711IleGly: 3.711 ± 0.503
1.026IleHis: 1.026 ± 0.297
1.895IleIle: 1.895 ± 0.462
3.79IleLys: 3.79 ± 0.489
3.474IleLeu: 3.474 ± 0.532
1.026IleMet: 1.026 ± 0.312
2.606IleAsn: 2.606 ± 0.582
2.448IlePro: 2.448 ± 0.54
1.737IleGln: 1.737 ± 0.401
3.553IleArg: 3.553 ± 0.572
2.211IleSer: 2.211 ± 0.529
4.027IleThr: 4.027 ± 0.465
4.185IleVal: 4.185 ± 0.506
0.474IleTrp: 0.474 ± 0.201
1.737IleTyr: 1.737 ± 0.333
0.0IleXaa: 0.0 ± 0.0
Lys
5.843LysAla: 5.843 ± 0.742
0.474LysCys: 0.474 ± 0.178
4.501LysAsp: 4.501 ± 0.611
3.79LysGlu: 3.79 ± 0.625
2.606LysPhe: 2.606 ± 0.399
4.816LysGly: 4.816 ± 0.574
1.184LysHis: 1.184 ± 0.4
1.816LysIle: 1.816 ± 0.367
3.158LysLys: 3.158 ± 0.605
5.448LysLeu: 5.448 ± 0.759
1.184LysMet: 1.184 ± 0.281
2.053LysAsn: 2.053 ± 0.396
2.685LysPro: 2.685 ± 0.602
2.527LysGln: 2.527 ± 0.48
3.316LysArg: 3.316 ± 0.645
2.448LysSer: 2.448 ± 0.441
2.842LysThr: 2.842 ± 0.49
5.369LysVal: 5.369 ± 0.662
0.553LysTrp: 0.553 ± 0.28
1.263LysTyr: 1.263 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
8.054LeuAla: 8.054 ± 0.8
0.553LeuCys: 0.553 ± 0.227
4.974LeuAsp: 4.974 ± 0.613
5.211LeuGlu: 5.211 ± 0.567
2.369LeuPhe: 2.369 ± 0.517
5.843LeuGly: 5.843 ± 0.763
2.211LeuHis: 2.211 ± 0.304
4.185LeuIle: 4.185 ± 0.533
6.632LeuLys: 6.632 ± 0.698
5.132LeuLeu: 5.132 ± 0.476
2.369LeuMet: 2.369 ± 0.363
3.474LeuAsn: 3.474 ± 0.651
2.764LeuPro: 2.764 ± 0.411
3.948LeuGln: 3.948 ± 0.678
5.606LeuArg: 5.606 ± 0.689
4.737LeuSer: 4.737 ± 0.734
4.895LeuThr: 4.895 ± 0.74
5.606LeuVal: 5.606 ± 0.749
0.79LeuTrp: 0.79 ± 0.27
1.737LeuTyr: 1.737 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.921MetAla: 2.921 ± 0.439
0.158MetCys: 0.158 ± 0.112
1.579MetAsp: 1.579 ± 0.304
2.053MetGlu: 2.053 ± 0.496
0.632MetPhe: 0.632 ± 0.258
2.053MetGly: 2.053 ± 0.396
0.474MetHis: 0.474 ± 0.178
1.579MetIle: 1.579 ± 0.246
1.5MetLys: 1.5 ± 0.333
2.527MetLeu: 2.527 ± 0.368
0.237MetMet: 0.237 ± 0.114
1.105MetAsn: 1.105 ± 0.338
1.421MetPro: 1.421 ± 0.329
0.632MetGln: 0.632 ± 0.168
0.79MetArg: 0.79 ± 0.275
2.29MetSer: 2.29 ± 0.446
2.211MetThr: 2.211 ± 0.411
1.263MetVal: 1.263 ± 0.336
0.158MetTrp: 0.158 ± 0.107
0.237MetTyr: 0.237 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.553AsnAla: 3.553 ± 0.506
0.553AsnCys: 0.553 ± 0.188
2.448AsnAsp: 2.448 ± 0.53
2.842AsnGlu: 2.842 ± 0.606
1.263AsnPhe: 1.263 ± 0.343
4.816AsnGly: 4.816 ± 0.955
0.395AsnHis: 0.395 ± 0.223
1.342AsnIle: 1.342 ± 0.329
0.79AsnLys: 0.79 ± 0.301
2.685AsnLeu: 2.685 ± 0.384
0.947AsnMet: 0.947 ± 0.352
1.658AsnAsn: 1.658 ± 0.347
2.685AsnPro: 2.685 ± 0.325
1.421AsnGln: 1.421 ± 0.289
2.211AsnArg: 2.211 ± 0.568
2.527AsnSer: 2.527 ± 0.526
1.737AsnThr: 1.737 ± 0.365
2.29AsnVal: 2.29 ± 0.429
0.869AsnTrp: 0.869 ± 0.243
1.263AsnTyr: 1.263 ± 0.33
0.0AsnXaa: 0.0 ± 0.0
Pro
3.869ProAla: 3.869 ± 0.553
0.316ProCys: 0.316 ± 0.164
3.316ProAsp: 3.316 ± 0.34
3.869ProGlu: 3.869 ± 0.506
1.342ProPhe: 1.342 ± 0.304
1.737ProGly: 1.737 ± 0.326
0.947ProHis: 0.947 ± 0.263
1.5ProIle: 1.5 ± 0.34
1.974ProLys: 1.974 ± 0.463
2.842ProLeu: 2.842 ± 0.542
0.79ProMet: 0.79 ± 0.227
2.132ProAsn: 2.132 ± 0.476
1.421ProPro: 1.421 ± 0.447
1.579ProGln: 1.579 ± 0.282
2.369ProArg: 2.369 ± 0.427
2.606ProSer: 2.606 ± 0.45
2.606ProThr: 2.606 ± 0.499
3.0ProVal: 3.0 ± 0.424
0.395ProTrp: 0.395 ± 0.257
1.421ProTyr: 1.421 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
6.001GlnAla: 6.001 ± 0.709
0.316GlnCys: 0.316 ± 0.163
2.764GlnAsp: 2.764 ± 0.478
3.158GlnGlu: 3.158 ± 0.655
1.974GlnPhe: 1.974 ± 0.329
3.553GlnGly: 3.553 ± 0.612
0.79GlnHis: 0.79 ± 0.254
1.816GlnIle: 1.816 ± 0.444
1.974GlnLys: 1.974 ± 0.728
3.948GlnLeu: 3.948 ± 0.486
0.947GlnMet: 0.947 ± 0.307
1.263GlnAsn: 1.263 ± 0.255
1.421GlnPro: 1.421 ± 0.301
2.921GlnGln: 2.921 ± 0.666
3.158GlnArg: 3.158 ± 0.535
2.053GlnSer: 2.053 ± 0.516
1.026GlnThr: 1.026 ± 0.265
2.369GlnVal: 2.369 ± 0.368
0.79GlnTrp: 0.79 ± 0.265
1.263GlnTyr: 1.263 ± 0.221
0.0GlnXaa: 0.0 ± 0.0
Arg
5.132ArgAla: 5.132 ± 0.591
0.316ArgCys: 0.316 ± 0.19
3.316ArgAsp: 3.316 ± 0.411
4.343ArgGlu: 4.343 ± 0.62
2.527ArgPhe: 2.527 ± 0.54
5.527ArgGly: 5.527 ± 0.599
1.342ArgHis: 1.342 ± 0.432
3.79ArgIle: 3.79 ± 0.55
3.632ArgLys: 3.632 ± 0.686
4.737ArgLeu: 4.737 ± 0.542
1.737ArgMet: 1.737 ± 0.31
2.369ArgAsn: 2.369 ± 0.421
1.579ArgPro: 1.579 ± 0.371
2.29ArgGln: 2.29 ± 0.534
3.474ArgArg: 3.474 ± 0.507
3.869ArgSer: 3.869 ± 0.584
2.764ArgThr: 2.764 ± 0.481
3.711ArgVal: 3.711 ± 0.485
0.947ArgTrp: 0.947 ± 0.241
2.448ArgTyr: 2.448 ± 0.331
0.0ArgXaa: 0.0 ± 0.0
Ser
5.527SerAla: 5.527 ± 0.561
0.395SerCys: 0.395 ± 0.179
4.659SerAsp: 4.659 ± 0.577
2.921SerGlu: 2.921 ± 0.397
2.685SerPhe: 2.685 ± 0.534
5.29SerGly: 5.29 ± 0.718
0.947SerHis: 0.947 ± 0.218
3.158SerIle: 3.158 ± 0.537
3.158SerLys: 3.158 ± 0.517
4.343SerLeu: 4.343 ± 0.539
0.947SerMet: 0.947 ± 0.243
1.895SerAsn: 1.895 ± 0.327
2.448SerPro: 2.448 ± 0.395
2.369SerGln: 2.369 ± 0.54
3.316SerArg: 3.316 ± 0.43
4.422SerSer: 4.422 ± 0.966
3.0SerThr: 3.0 ± 0.52
3.869SerVal: 3.869 ± 0.568
0.632SerTrp: 0.632 ± 0.209
2.053SerTyr: 2.053 ± 0.431
0.0SerXaa: 0.0 ± 0.0
Thr
4.58ThrAla: 4.58 ± 0.651
0.316ThrCys: 0.316 ± 0.148
3.869ThrAsp: 3.869 ± 0.647
2.764ThrGlu: 2.764 ± 0.525
2.132ThrPhe: 2.132 ± 0.454
4.027ThrGly: 4.027 ± 0.513
0.869ThrHis: 0.869 ± 0.283
4.501ThrIle: 4.501 ± 0.859
4.895ThrLys: 4.895 ± 0.439
4.027ThrLeu: 4.027 ± 0.487
1.263ThrMet: 1.263 ± 0.313
2.369ThrAsn: 2.369 ± 0.571
2.842ThrPro: 2.842 ± 0.375
2.211ThrGln: 2.211 ± 0.419
3.395ThrArg: 3.395 ± 0.582
3.395ThrSer: 3.395 ± 0.701
3.316ThrThr: 3.316 ± 0.631
4.027ThrVal: 4.027 ± 0.501
0.395ThrTrp: 0.395 ± 0.191
1.737ThrTyr: 1.737 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
6.001ValAla: 6.001 ± 0.717
0.553ValCys: 0.553 ± 0.193
3.237ValAsp: 3.237 ± 0.55
4.895ValGlu: 4.895 ± 0.797
1.5ValPhe: 1.5 ± 0.275
4.185ValGly: 4.185 ± 0.624
1.105ValHis: 1.105 ± 0.333
3.237ValIle: 3.237 ± 0.576
3.711ValLys: 3.711 ± 0.506
6.159ValLeu: 6.159 ± 0.594
1.737ValMet: 1.737 ± 0.413
2.448ValAsn: 2.448 ± 0.487
3.711ValPro: 3.711 ± 0.687
2.211ValGln: 2.211 ± 0.434
4.501ValArg: 4.501 ± 0.558
4.185ValSer: 4.185 ± 0.559
4.737ValThr: 4.737 ± 0.695
5.843ValVal: 5.843 ± 0.821
0.947ValTrp: 0.947 ± 0.254
2.764ValTyr: 2.764 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
1.421TrpAla: 1.421 ± 0.354
0.158TrpCys: 0.158 ± 0.12
1.026TrpAsp: 1.026 ± 0.33
0.869TrpGlu: 0.869 ± 0.288
0.947TrpPhe: 0.947 ± 0.24
0.947TrpGly: 0.947 ± 0.308
0.711TrpHis: 0.711 ± 0.228
0.632TrpIle: 0.632 ± 0.204
0.79TrpLys: 0.79 ± 0.152
1.737TrpLeu: 1.737 ± 0.424
0.316TrpMet: 0.316 ± 0.139
0.316TrpAsn: 0.316 ± 0.144
0.395TrpPro: 0.395 ± 0.187
0.869TrpGln: 0.869 ± 0.303
0.632TrpArg: 0.632 ± 0.201
0.79TrpSer: 0.79 ± 0.224
0.947TrpThr: 0.947 ± 0.31
0.79TrpVal: 0.79 ± 0.241
0.158TrpTrp: 0.158 ± 0.119
0.316TrpTyr: 0.316 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.921TyrAla: 2.921 ± 0.534
0.316TyrCys: 0.316 ± 0.125
1.816TyrAsp: 1.816 ± 0.514
2.053TyrGlu: 2.053 ± 0.346
1.184TyrPhe: 1.184 ± 0.301
3.158TyrGly: 3.158 ± 0.561
0.395TyrHis: 0.395 ± 0.176
1.263TyrIle: 1.263 ± 0.402
1.974TyrLys: 1.974 ± 0.38
2.921TyrLeu: 2.921 ± 0.426
0.947TyrMet: 0.947 ± 0.252
1.263TyrAsn: 1.263 ± 0.205
1.184TyrPro: 1.184 ± 0.252
2.369TyrGln: 2.369 ± 0.463
1.658TyrArg: 1.658 ± 0.281
1.421TyrSer: 1.421 ± 0.329
1.421TyrThr: 1.421 ± 0.34
1.737TyrVal: 1.737 ± 0.307
0.553TyrTrp: 0.553 ± 0.234
0.632TyrTyr: 0.632 ± 0.255
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski