Bacillus wakoensis JCM 9140

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus wakoensis

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4575 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4Q8D0|W4Q8D0_9BACI Uncharacterized protein OS=Bacillus wakoensis JCM 9140 OX=1236970 GN=JCM9140_4543 PE=4 SV=1
MM1 pKa = 7.56LKK3 pKa = 10.22KK4 pKa = 10.37KK5 pKa = 9.51WGTYY9 pKa = 8.36VLAGAFTLMMTACGSSTEE27 pKa = 4.29TEE29 pKa = 4.3STSEE33 pKa = 4.24SVDD36 pKa = 3.49APSDD40 pKa = 3.46DD41 pKa = 4.07PEE43 pKa = 4.54PVEE46 pKa = 4.38EE47 pKa = 4.22TVEE50 pKa = 4.09MTDD53 pKa = 3.47VKK55 pKa = 11.03QVTNWFAQPEE65 pKa = 4.39HH66 pKa = 6.56GGLYY70 pKa = 10.45AALEE74 pKa = 4.02KK75 pKa = 10.96GFYY78 pKa = 9.84EE79 pKa = 4.14EE80 pKa = 5.17NGMNMSIDD88 pKa = 3.22QGGPQVSSIQIVASGQAQFGLAQADD113 pKa = 4.29DD114 pKa = 3.7ILIARR119 pKa = 11.84AEE121 pKa = 4.18GVPIVAIGTIFQTSPQGLLFHH142 pKa = 6.96NSQGITDD149 pKa = 4.34FADD152 pKa = 3.35LQGRR156 pKa = 11.84KK157 pKa = 9.96GIIQPGIGYY166 pKa = 8.88WEE168 pKa = 4.38YY169 pKa = 10.71IKK171 pKa = 10.68EE172 pKa = 4.25FYY174 pKa = 11.01DD175 pKa = 4.03LDD177 pKa = 4.1DD178 pKa = 3.8VQEE181 pKa = 4.13ITFTGQFIDD190 pKa = 4.94FIEE193 pKa = 5.41DD194 pKa = 3.29EE195 pKa = 4.41TSFTQGYY202 pKa = 6.57VTQDD206 pKa = 3.22PFNFEE211 pKa = 4.01QQGLDD216 pKa = 3.11TDD218 pKa = 3.86YY219 pKa = 11.91LLIHH223 pKa = 7.34DD224 pKa = 5.09SGYY227 pKa = 10.28QPYY230 pKa = 10.12ANVMFTTEE238 pKa = 4.38EE239 pKa = 4.34LIEE242 pKa = 4.3NNPEE246 pKa = 3.77LVSAYY251 pKa = 10.54VEE253 pKa = 4.09ASVKK257 pKa = 9.89GWEE260 pKa = 4.55FYY262 pKa = 10.71KK263 pKa = 10.83EE264 pKa = 4.18NYY266 pKa = 10.23DD267 pKa = 3.85EE268 pKa = 4.69INPVIQEE275 pKa = 4.28SNPDD279 pKa = 3.42FPTDD283 pKa = 3.41QLAFTAEE290 pKa = 4.2TLVTYY295 pKa = 10.7AFEE298 pKa = 5.17GDD300 pKa = 3.52AAEE303 pKa = 4.68HH304 pKa = 6.19GFGYY308 pKa = 9.93MSEE311 pKa = 4.3EE312 pKa = 3.89RR313 pKa = 11.84WTTLHH318 pKa = 6.05EE319 pKa = 4.05QLAEE323 pKa = 4.07LEE325 pKa = 4.74IITPLDD331 pKa = 3.47DD332 pKa = 3.61VGASFTNDD340 pKa = 3.6FLPGHH345 pKa = 6.89

Molecular weight: 38.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4Q2N6|W4Q2N6_9BACI Uncharacterized protein OS=Bacillus wakoensis JCM 9140 OX=1236970 GN=JCM9140_2300 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4575

0

4575

1243538

37

1502

271.8

30.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.858 ± 0.042

0.713 ± 0.012

5.075 ± 0.031

7.965 ± 0.042

4.525 ± 0.03

6.704 ± 0.038

2.22 ± 0.019

7.557 ± 0.03

6.446 ± 0.036

9.848 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.017

4.204 ± 0.027

3.534 ± 0.022

4.036 ± 0.029

4.201 ± 0.026

6.045 ± 0.03

5.525 ± 0.024

7.385 ± 0.031

1.001 ± 0.014

3.34 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski