Amino acid dipepetide frequency for Bacillus wakoensis JCM 9140

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.381AlaAla: 5.381 ± 0.081
0.619AlaCys: 0.619 ± 0.022
3.219AlaAsp: 3.219 ± 0.052
4.604AlaGlu: 4.604 ± 0.074
3.209AlaPhe: 3.209 ± 0.057
4.951AlaGly: 4.951 ± 0.069
1.345AlaHis: 1.345 ± 0.033
5.925AlaIle: 5.925 ± 0.071
4.433AlaLys: 4.433 ± 0.068
7.155AlaLeu: 7.155 ± 0.092
1.996AlaMet: 1.996 ± 0.042
2.922AlaAsn: 2.922 ± 0.053
1.985AlaPro: 1.985 ± 0.047
2.24AlaGln: 2.24 ± 0.04
2.647AlaArg: 2.647 ± 0.041
4.129AlaSer: 4.129 ± 0.065
3.894AlaThr: 3.894 ± 0.062
5.161AlaVal: 5.161 ± 0.08
0.556AlaTrp: 0.556 ± 0.024
2.21AlaTyr: 2.21 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.019
0.097CysCys: 0.097 ± 0.008
0.359CysAsp: 0.359 ± 0.017
0.459CysGlu: 0.459 ± 0.023
0.33CysPhe: 0.33 ± 0.016
0.68CysGly: 0.68 ± 0.027
0.205CysHis: 0.205 ± 0.011
0.475CysIle: 0.475 ± 0.018
0.397CysLys: 0.397 ± 0.019
0.696CysLeu: 0.696 ± 0.024
0.179CysMet: 0.179 ± 0.012
0.269CysAsn: 0.269 ± 0.017
0.369CysPro: 0.369 ± 0.019
0.264CysGln: 0.264 ± 0.016
0.292CysArg: 0.292 ± 0.017
0.514CysSer: 0.514 ± 0.019
0.408CysThr: 0.408 ± 0.017
0.43CysVal: 0.43 ± 0.02
0.064CysTrp: 0.064 ± 0.007
0.242CysTyr: 0.242 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.057AspAla: 3.057 ± 0.056
0.371AspCys: 0.371 ± 0.019
2.593AspAsp: 2.593 ± 0.052
4.651AspGlu: 4.651 ± 0.072
2.367AspPhe: 2.367 ± 0.044
3.342AspGly: 3.342 ± 0.059
1.353AspHis: 1.353 ± 0.036
3.666AspIle: 3.666 ± 0.061
2.589AspLys: 2.589 ± 0.051
5.332AspLeu: 5.332 ± 0.064
1.279AspMet: 1.279 ± 0.03
1.596AspAsn: 1.596 ± 0.037
1.944AspPro: 1.944 ± 0.041
2.361AspGln: 2.361 ± 0.043
2.322AspArg: 2.322 ± 0.043
2.602AspSer: 2.602 ± 0.046
2.322AspThr: 2.322 ± 0.046
4.371AspVal: 4.371 ± 0.065
0.613AspTrp: 0.613 ± 0.019
2.019AspTyr: 2.019 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
5.669GluAla: 5.669 ± 0.078
0.394GluCys: 0.394 ± 0.018
3.964GluAsp: 3.964 ± 0.063
8.153GluGlu: 8.153 ± 0.108
2.561GluPhe: 2.561 ± 0.051
4.586GluGly: 4.586 ± 0.062
1.754GluHis: 1.754 ± 0.041
5.537GluIle: 5.537 ± 0.066
6.326GluLys: 6.326 ± 0.087
7.657GluLeu: 7.657 ± 0.082
2.488GluMet: 2.488 ± 0.042
3.447GluAsn: 3.447 ± 0.048
2.113GluPro: 2.113 ± 0.045
4.182GluGln: 4.182 ± 0.073
4.075GluArg: 4.075 ± 0.058
3.833GluSer: 3.833 ± 0.06
4.19GluThr: 4.19 ± 0.056
5.718GluVal: 5.718 ± 0.083
0.863GluTrp: 0.863 ± 0.031
2.14GluTyr: 2.14 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.041PheAla: 3.041 ± 0.058
0.335PheCys: 0.335 ± 0.016
2.444PheAsp: 2.444 ± 0.049
3.061PheGlu: 3.061 ± 0.049
2.442PhePhe: 2.442 ± 0.053
3.242PheGly: 3.242 ± 0.056
1.086PheHis: 1.086 ± 0.028
3.681PheIle: 3.681 ± 0.061
2.155PheLys: 2.155 ± 0.042
4.746PheLeu: 4.746 ± 0.067
1.156PheMet: 1.156 ± 0.031
1.622PheAsn: 1.622 ± 0.035
1.599PhePro: 1.599 ± 0.039
1.773PheGln: 1.773 ± 0.04
1.56PheArg: 1.56 ± 0.033
3.251PheSer: 3.251 ± 0.053
2.549PheThr: 2.549 ± 0.047
3.374PheVal: 3.374 ± 0.053
0.451PheTrp: 0.451 ± 0.02
1.68PheTyr: 1.68 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
4.79GlyAla: 4.79 ± 0.078
0.617GlyCys: 0.617 ± 0.023
3.086GlyAsp: 3.086 ± 0.055
4.633GlyGlu: 4.633 ± 0.061
3.396GlyPhe: 3.396 ± 0.055
4.617GlyGly: 4.617 ± 0.081
1.443GlyHis: 1.443 ± 0.039
5.488GlyIle: 5.488 ± 0.061
4.441GlyLys: 4.441 ± 0.059
6.602GlyLeu: 6.602 ± 0.091
2.16GlyMet: 2.16 ± 0.042
2.499GlyAsn: 2.499 ± 0.052
1.782GlyPro: 1.782 ± 0.04
2.341GlyGln: 2.341 ± 0.047
2.646GlyArg: 2.646 ± 0.046
3.883GlySer: 3.883 ± 0.064
3.942GlyThr: 3.942 ± 0.06
5.262GlyVal: 5.262 ± 0.075
0.739GlyTrp: 0.739 ± 0.027
2.673GlyTyr: 2.673 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.384HisAla: 1.384 ± 0.035
0.216HisCys: 0.216 ± 0.014
1.157HisAsp: 1.157 ± 0.034
1.606HisGlu: 1.606 ± 0.036
1.152HisPhe: 1.152 ± 0.035
1.385HisGly: 1.385 ± 0.035
0.748HisHis: 0.748 ± 0.031
1.585HisIle: 1.585 ± 0.038
1.024HisLys: 1.024 ± 0.031
2.308HisLeu: 2.308 ± 0.043
0.54HisMet: 0.54 ± 0.021
0.828HisAsn: 0.828 ± 0.023
1.144HisPro: 1.144 ± 0.032
0.93HisGln: 0.93 ± 0.027
0.869HisArg: 0.869 ± 0.027
1.397HisSer: 1.397 ± 0.032
1.11HisThr: 1.11 ± 0.032
1.675HisVal: 1.675 ± 0.037
0.246HisTrp: 0.246 ± 0.012
0.897HisTyr: 0.897 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.712IleAla: 5.712 ± 0.073
0.589IleCys: 0.589 ± 0.021
4.368IleAsp: 4.368 ± 0.066
6.141IleGlu: 6.141 ± 0.078
2.926IlePhe: 2.926 ± 0.061
5.992IleGly: 5.992 ± 0.084
1.727IleHis: 1.727 ± 0.045
5.467IleIle: 5.467 ± 0.077
4.185IleLys: 4.185 ± 0.065
6.542IleLeu: 6.542 ± 0.083
1.768IleMet: 1.768 ± 0.04
3.046IleAsn: 3.046 ± 0.053
3.25IlePro: 3.25 ± 0.058
3.007IleGln: 3.007 ± 0.052
3.187IleArg: 3.187 ± 0.055
4.86IleSer: 4.86 ± 0.059
4.235IleThr: 4.235 ± 0.061
5.699IleVal: 5.699 ± 0.067
0.672IleTrp: 0.672 ± 0.028
2.195IleTyr: 2.195 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.244LysAla: 4.244 ± 0.065
0.309LysCys: 0.309 ± 0.019
3.347LysAsp: 3.347 ± 0.054
6.876LysGlu: 6.876 ± 0.092
1.624LysPhe: 1.624 ± 0.04
4.238LysGly: 4.238 ± 0.062
1.312LysHis: 1.312 ± 0.035
3.867LysIle: 3.867 ± 0.055
5.736LysLys: 5.736 ± 0.086
5.352LysLeu: 5.352 ± 0.072
2.071LysMet: 2.071 ± 0.039
2.808LysAsn: 2.808 ± 0.051
2.113LysPro: 2.113 ± 0.046
3.332LysGln: 3.332 ± 0.062
3.494LysArg: 3.494 ± 0.058
3.246LysSer: 3.246 ± 0.053
3.271LysThr: 3.271 ± 0.051
4.644LysVal: 4.644 ± 0.06
0.799LysTrp: 0.799 ± 0.027
1.78LysTyr: 1.78 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
7.399LeuAla: 7.399 ± 0.081
0.672LeuCys: 0.672 ± 0.024
4.925LeuAsp: 4.925 ± 0.057
7.251LeuGlu: 7.251 ± 0.08
5.051LeuPhe: 5.051 ± 0.081
6.307LeuGly: 6.307 ± 0.084
2.159LeuHis: 2.159 ± 0.052
7.114LeuIle: 7.114 ± 0.096
6.043LeuLys: 6.043 ± 0.065
10.46LeuLeu: 10.46 ± 0.123
2.49LeuMet: 2.49 ± 0.044
3.986LeuAsn: 3.986 ± 0.051
3.831LeuPro: 3.831 ± 0.054
3.991LeuGln: 3.991 ± 0.063
3.775LeuArg: 3.775 ± 0.06
6.684LeuSer: 6.684 ± 0.079
5.813LeuThr: 5.813 ± 0.067
6.728LeuVal: 6.728 ± 0.075
0.77LeuTrp: 0.77 ± 0.027
3.032LeuTyr: 3.032 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
1.985MetAla: 1.985 ± 0.039
0.138MetCys: 0.138 ± 0.011
1.358MetAsp: 1.358 ± 0.035
1.991MetGlu: 1.991 ± 0.036
1.168MetPhe: 1.168 ± 0.031
1.706MetGly: 1.706 ± 0.037
0.436MetHis: 0.436 ± 0.019
2.264MetIle: 2.264 ± 0.044
2.515MetLys: 2.515 ± 0.043
2.573MetLeu: 2.573 ± 0.043
0.914MetMet: 0.914 ± 0.029
1.668MetAsn: 1.668 ± 0.039
0.946MetPro: 0.946 ± 0.027
0.893MetGln: 0.893 ± 0.029
1.11MetArg: 1.11 ± 0.029
1.881MetSer: 1.881 ± 0.044
1.773MetThr: 1.773 ± 0.042
1.854MetVal: 1.854 ± 0.036
0.212MetTrp: 0.212 ± 0.015
0.806MetTyr: 0.806 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.485AsnAla: 2.485 ± 0.049
0.294AsnCys: 0.294 ± 0.017
2.277AsnAsp: 2.277 ± 0.044
3.795AsnGlu: 3.795 ± 0.059
1.429AsnPhe: 1.429 ± 0.038
3.158AsnGly: 3.158 ± 0.053
1.099AsnHis: 1.099 ± 0.03
3.003AsnIle: 3.003 ± 0.056
2.58AsnLys: 2.58 ± 0.056
3.637AsnLeu: 3.637 ± 0.063
1.222AsnMet: 1.222 ± 0.037
1.719AsnAsn: 1.719 ± 0.039
2.014AsnPro: 2.014 ± 0.044
2.156AsnGln: 2.156 ± 0.046
2.001AsnArg: 2.001 ± 0.039
2.125AsnSer: 2.125 ± 0.044
1.915AsnThr: 1.915 ± 0.045
3.211AsnVal: 3.211 ± 0.05
0.535AsnTrp: 0.535 ± 0.019
1.384AsnTyr: 1.384 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
2.192ProAla: 2.192 ± 0.045
0.22ProCys: 0.22 ± 0.014
1.866ProAsp: 1.866 ± 0.039
2.851ProGlu: 2.851 ± 0.05
2.039ProPhe: 2.039 ± 0.041
2.146ProGly: 2.146 ± 0.048
0.811ProHis: 0.811 ± 0.025
2.928ProIle: 2.928 ± 0.056
2.007ProLys: 2.007 ± 0.038
3.595ProLeu: 3.595 ± 0.055
0.849ProMet: 0.849 ± 0.028
1.706ProAsn: 1.706 ± 0.037
1.012ProPro: 1.012 ± 0.031
1.081ProGln: 1.081 ± 0.03
1.103ProArg: 1.103 ± 0.032
2.318ProSer: 2.318 ± 0.045
2.18ProThr: 2.18 ± 0.038
2.727ProVal: 2.727 ± 0.05
0.379ProTrp: 0.379 ± 0.019
1.328ProTyr: 1.328 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.956GlnAla: 2.956 ± 0.055
0.207GlnCys: 0.207 ± 0.014
1.705GlnAsp: 1.705 ± 0.036
3.182GlnGlu: 3.182 ± 0.051
1.852GlnPhe: 1.852 ± 0.034
2.205GlnGly: 2.205 ± 0.041
0.885GlnHis: 0.885 ± 0.024
2.798GlnIle: 2.798 ± 0.049
2.716GlnLys: 2.716 ± 0.046
4.553GlnLeu: 4.553 ± 0.073
1.312GlnMet: 1.312 ± 0.037
1.631GlnAsn: 1.631 ± 0.039
1.287GlnPro: 1.287 ± 0.037
2.095GlnGln: 2.095 ± 0.068
1.594GlnArg: 1.594 ± 0.04
2.486GlnSer: 2.486 ± 0.052
2.27GlnThr: 2.27 ± 0.047
2.907GlnVal: 2.907 ± 0.05
0.429GlnTrp: 0.429 ± 0.02
1.29GlnTyr: 1.29 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.599ArgAla: 2.599 ± 0.05
0.262ArgCys: 0.262 ± 0.015
2.179ArgAsp: 2.179 ± 0.043
3.418ArgGlu: 3.418 ± 0.052
2.117ArgPhe: 2.117 ± 0.041
2.525ArgGly: 2.525 ± 0.044
0.8ArgHis: 0.8 ± 0.026
3.02ArgIle: 3.02 ± 0.047
3.201ArgLys: 3.201 ± 0.057
4.179ArgLeu: 4.179 ± 0.056
1.309ArgMet: 1.309 ± 0.029
2.003ArgAsn: 2.003 ± 0.044
1.386ArgPro: 1.386 ± 0.037
1.618ArgGln: 1.618 ± 0.041
1.858ArgArg: 1.858 ± 0.045
2.41ArgSer: 2.41 ± 0.047
2.244ArgThr: 2.244 ± 0.043
2.872ArgVal: 2.872 ± 0.053
0.436ArgTrp: 0.436 ± 0.019
1.571ArgTyr: 1.571 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
3.611SerAla: 3.611 ± 0.062
0.42SerCys: 0.42 ± 0.022
2.815SerAsp: 2.815 ± 0.053
4.103SerGlu: 4.103 ± 0.061
3.389SerPhe: 3.389 ± 0.053
4.12SerGly: 4.12 ± 0.06
1.313SerHis: 1.313 ± 0.036
5.211SerIle: 5.211 ± 0.068
3.683SerLys: 3.683 ± 0.057
6.286SerLeu: 6.286 ± 0.069
1.804SerMet: 1.804 ± 0.037
2.642SerAsn: 2.642 ± 0.052
2.072SerPro: 2.072 ± 0.042
2.092SerGln: 2.092 ± 0.044
2.395SerArg: 2.395 ± 0.042
3.966SerSer: 3.966 ± 0.063
3.31SerThr: 3.31 ± 0.049
4.359SerVal: 4.359 ± 0.066
0.628SerTrp: 0.628 ± 0.026
2.232SerTyr: 2.232 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
3.701ThrAla: 3.701 ± 0.062
0.392ThrCys: 0.392 ± 0.02
2.709ThrAsp: 2.709 ± 0.054
3.858ThrGlu: 3.858 ± 0.064
2.825ThrPhe: 2.825 ± 0.048
3.965ThrGly: 3.965 ± 0.062
1.143ThrHis: 1.143 ± 0.028
4.714ThrIle: 4.714 ± 0.055
3.381ThrLys: 3.381 ± 0.056
5.356ThrLeu: 5.356 ± 0.073
1.467ThrMet: 1.467 ± 0.033
2.644ThrAsn: 2.644 ± 0.057
2.149ThrPro: 2.149 ± 0.045
1.538ThrGln: 1.538 ± 0.038
2.061ThrArg: 2.061 ± 0.04
3.375ThrSer: 3.375 ± 0.052
3.127ThrThr: 3.127 ± 0.054
4.338ThrVal: 4.338 ± 0.06
0.517ThrTrp: 0.517 ± 0.019
1.989ThrTyr: 1.989 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
5.477ValAla: 5.477 ± 0.086
0.602ValCys: 0.602 ± 0.02
3.968ValAsp: 3.968 ± 0.06
5.637ValGlu: 5.637 ± 0.085
3.17ValPhe: 3.17 ± 0.056
4.931ValGly: 4.931 ± 0.071
1.49ValHis: 1.49 ± 0.036
5.804ValIle: 5.804 ± 0.065
4.518ValLys: 4.518 ± 0.068
6.994ValLeu: 6.994 ± 0.086
1.977ValMet: 1.977 ± 0.038
3.139ValAsn: 3.139 ± 0.052
2.745ValPro: 2.745 ± 0.05
2.567ValGln: 2.567 ± 0.051
3.03ValArg: 3.03 ± 0.046
4.812ValSer: 4.812 ± 0.074
4.611ValThr: 4.611 ± 0.072
5.481ValVal: 5.481 ± 0.078
0.606ValTrp: 0.606 ± 0.026
2.295ValTyr: 2.295 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.59TrpAla: 0.59 ± 0.021
0.067TrpCys: 0.067 ± 0.007
0.511TrpAsp: 0.511 ± 0.022
0.739TrpGlu: 0.739 ± 0.024
0.496TrpPhe: 0.496 ± 0.021
0.64TrpGly: 0.64 ± 0.022
0.211TrpHis: 0.211 ± 0.015
0.753TrpIle: 0.753 ± 0.026
0.693TrpLys: 0.693 ± 0.027
1.158TrpLeu: 1.158 ± 0.031
0.329TrpMet: 0.329 ± 0.017
0.515TrpAsn: 0.515 ± 0.022
0.274TrpPro: 0.274 ± 0.017
0.384TrpGln: 0.384 ± 0.018
0.384TrpArg: 0.384 ± 0.017
0.65TrpSer: 0.65 ± 0.023
0.479TrpThr: 0.479 ± 0.02
0.661TrpVal: 0.661 ± 0.029
0.148TrpTrp: 0.148 ± 0.014
0.327TrpTyr: 0.327 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.914TyrAla: 1.914 ± 0.043
0.308TyrCys: 0.308 ± 0.018
1.903TyrAsp: 1.903 ± 0.04
2.641TyrGlu: 2.641 ± 0.05
1.707TyrPhe: 1.707 ± 0.039
2.306TyrGly: 2.306 ± 0.038
0.879TyrHis: 0.879 ± 0.03
2.265TyrIle: 2.265 ± 0.048
1.838TyrLys: 1.838 ± 0.042
3.303TyrLeu: 3.303 ± 0.056
0.88TyrMet: 0.88 ± 0.028
1.346TyrAsn: 1.346 ± 0.032
1.323TyrPro: 1.323 ± 0.034
1.517TyrGln: 1.517 ± 0.035
1.602TyrArg: 1.602 ± 0.033
2.028TyrSer: 2.028 ± 0.042
1.605TyrThr: 1.605 ± 0.035
2.38TyrVal: 2.38 ± 0.045
0.346TyrTrp: 0.346 ± 0.015
1.308TyrTyr: 1.308 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4575 proteins (1243538 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski