Amino acid dipepetide frequency for Rhodotorula graminis (strain WP1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.148AlaAla: 18.148 ± 0.152	1.137AlaCys: 1.137 ± 0.023	5.85AlaAsp: 5.85 ± 0.041	5.574AlaGlu: 5.574 ± 0.049	3.675AlaPhe: 3.675 ± 0.036	8.745AlaGly: 8.745 ± 0.065	3.068AlaHis: 3.068 ± 0.033	3.268AlaIle: 3.268 ± 0.037	4.239AlaLys: 4.239 ± 0.048	11.506AlaLeu: 11.506 ± 0.081	1.854AlaMet: 1.854 ± 0.025	2.338AlaAsn: 2.338 ± 0.026	9.754AlaPro: 9.754 ± 0.098	4.599AlaGln: 4.599 ± 0.046	8.773AlaArg: 8.773 ± 0.072	11.027AlaSer: 11.027 ± 0.092	6.453AlaThr: 6.453 ± 0.05	7.226AlaVal: 7.226 ± 0.05	1.337AlaTrp: 1.337 ± 0.021	2.252AlaTyr: 2.252 ± 0.028	0.0AlaXaa: 0.0 ± 0.0
Cys	1.155CysAla: 1.155 ± 0.023	0.18CysCys: 0.18 ± 0.007	0.573CysAsp: 0.573 ± 0.012	0.491CysGlu: 0.491 ± 0.014	0.388CysPhe: 0.388 ± 0.012	0.877CysGly: 0.877 ± 0.018	0.22CysHis: 0.22 ± 0.009	0.429CysIle: 0.429 ± 0.011	0.328CysLys: 0.328 ± 0.01	0.954CysLeu: 0.954 ± 0.017	0.172CysMet: 0.172 ± 0.01	0.233CysAsn: 0.233 ± 0.008	0.592CysPro: 0.592 ± 0.014	0.264CysGln: 0.264 ± 0.009	0.711CysArg: 0.711 ± 0.017	0.721CysSer: 0.721 ± 0.017	0.586CysThr: 0.586 ± 0.014	0.742CysVal: 0.742 ± 0.015	0.144CysTrp: 0.144 ± 0.006	0.245CysTyr: 0.245 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.508AspAla: 6.508 ± 0.049	0.469AspCys: 0.469 ± 0.013	6.126AspAsp: 6.126 ± 0.079	6.019AspGlu: 6.019 ± 0.063	1.766AspPhe: 1.766 ± 0.024	4.766AspGly: 4.766 ± 0.05	1.115AspHis: 1.115 ± 0.018	1.711AspIle: 1.711 ± 0.025	2.556AspLys: 2.556 ± 0.03	5.554AspLeu: 5.554 ± 0.046	0.905AspMet: 0.905 ± 0.015	1.151AspAsn: 1.151 ± 0.017	3.622AspPro: 3.622 ± 0.038	1.567AspGln: 1.567 ± 0.02	3.152AspArg: 3.152 ± 0.036	3.788AspSer: 3.788 ± 0.035	2.289AspThr: 2.289 ± 0.024	3.996AspVal: 3.996 ± 0.034	0.778AspTrp: 0.778 ± 0.015	1.218AspTyr: 1.218 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	6.096GluAla: 6.096 ± 0.053	0.518GluCys: 0.518 ± 0.013	3.777GluAsp: 3.777 ± 0.043	4.27GluGlu: 4.27 ± 0.048	1.446GluPhe: 1.446 ± 0.019	4.851GluGly: 4.851 ± 0.053	1.547GluHis: 1.547 ± 0.021	1.775GluIle: 1.775 ± 0.024	2.162GluLys: 2.162 ± 0.027	5.577GluLeu: 5.577 ± 0.046	1.058GluMet: 1.058 ± 0.017	1.1GluAsn: 1.1 ± 0.019	3.117GluPro: 3.117 ± 0.04	2.517GluGln: 2.517 ± 0.033	5.559GluArg: 5.559 ± 0.05	3.081GluSer: 3.081 ± 0.031	2.506GluThr: 2.506 ± 0.026	3.802GluVal: 3.802 ± 0.033	0.892GluTrp: 0.892 ± 0.015	1.242GluTyr: 1.242 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.707PheAla: 3.707 ± 0.035	0.438PheCys: 0.438 ± 0.011	2.182PheAsp: 2.182 ± 0.026	1.857PheGlu: 1.857 ± 0.026	1.329PhePhe: 1.329 ± 0.024	2.633PheGly: 2.633 ± 0.037	0.742PheHis: 0.742 ± 0.014	1.122PheIle: 1.122 ± 0.021	1.139PheLys: 1.139 ± 0.019	3.228PheLeu: 3.228 ± 0.039	0.471PheMet: 0.471 ± 0.013	0.895PheAsn: 0.895 ± 0.018	1.834PhePro: 1.834 ± 0.025	0.877PheGln: 0.877 ± 0.015	1.706PheArg: 1.706 ± 0.024	2.608PheSer: 2.608 ± 0.032	1.624PheThr: 1.624 ± 0.024	2.446PheVal: 2.446 ± 0.03	0.435PheTrp: 0.435 ± 0.012	0.807PheTyr: 0.807 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	9.449GlyAla: 9.449 ± 0.076	0.679GlyCys: 0.679 ± 0.015	4.327GlyAsp: 4.327 ± 0.04	4.679GlyGlu: 4.679 ± 0.045	2.451GlyPhe: 2.451 ± 0.034	9.257GlyGly: 9.257 ± 0.117	1.603GlyHis: 1.603 ± 0.025	2.437GlyIle: 2.437 ± 0.031	3.387GlyLys: 3.387 ± 0.04	6.071GlyLeu: 6.071 ± 0.053	1.296GlyMet: 1.296 ± 0.025	1.556GlyAsn: 1.556 ± 0.024	4.043GlyPro: 4.043 ± 0.04	2.449GlyGln: 2.449 ± 0.03	5.258GlyArg: 5.258 ± 0.048	5.864GlySer: 5.864 ± 0.06	3.823GlyThr: 3.823 ± 0.038	4.76GlyVal: 4.76 ± 0.039	1.154GlyTrp: 1.154 ± 0.022	1.65GlyTyr: 1.65 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.794HisAla: 2.794 ± 0.036	0.241HisCys: 0.241 ± 0.009	1.549HisAsp: 1.549 ± 0.023	1.348HisGlu: 1.348 ± 0.021	0.804HisPhe: 0.804 ± 0.016	1.851HisGly: 1.851 ± 0.029	0.935HisHis: 0.935 ± 0.022	0.709HisIle: 0.709 ± 0.017	0.778HisLys: 0.778 ± 0.016	2.779HisLeu: 2.779 ± 0.027	0.319HisMet: 0.319 ± 0.013	0.519HisAsn: 0.519 ± 0.011	1.971HisPro: 1.971 ± 0.027	0.827HisGln: 0.827 ± 0.02	1.611HisArg: 1.611 ± 0.023	1.659HisSer: 1.659 ± 0.024	1.043HisThr: 1.043 ± 0.019	1.645HisVal: 1.645 ± 0.024	0.287HisTrp: 0.287 ± 0.016	0.557HisTyr: 0.557 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.233IleAla: 3.233 ± 0.034	0.418IleCys: 0.418 ± 0.011	1.982IleAsp: 1.982 ± 0.024	1.911IleGlu: 1.911 ± 0.027	1.155IlePhe: 1.155 ± 0.023	2.173IleGly: 2.173 ± 0.03	0.668IleHis: 0.668 ± 0.014	1.201IleIle: 1.201 ± 0.024	1.387IleLys: 1.387 ± 0.03	3.051IleLeu: 3.051 ± 0.042	0.537IleMet: 0.537 ± 0.014	0.898IleAsn: 0.898 ± 0.02	1.846IlePro: 1.846 ± 0.025	0.974IleGln: 0.974 ± 0.018	1.837IleArg: 1.837 ± 0.025	2.229IleSer: 2.229 ± 0.029	1.544IleThr: 1.544 ± 0.027	2.51IleVal: 2.51 ± 0.031	0.395IleTrp: 0.395 ± 0.011	0.746IleTyr: 0.746 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.248LysAla: 4.248 ± 0.049	0.315LysCys: 0.315 ± 0.011	2.127LysAsp: 2.127 ± 0.03	2.423LysGlu: 2.423 ± 0.031	0.952LysPhe: 0.952 ± 0.018	3.075LysGly: 3.075 ± 0.04	0.864LysHis: 0.864 ± 0.016	1.247LysIle: 1.247 ± 0.024	2.596LysLys: 2.596 ± 0.05	3.367LysLeu: 3.367 ± 0.037	0.691LysMet: 0.691 ± 0.016	0.919LysAsn: 0.919 ± 0.017	2.211LysPro: 2.211 ± 0.031	1.399LysGln: 1.399 ± 0.025	3.213LysArg: 3.213 ± 0.038	2.23LysSer: 2.23 ± 0.031	1.907LysThr: 1.907 ± 0.027	2.67LysVal: 2.67 ± 0.036	0.47LysTrp: 0.47 ± 0.013	0.817LysTyr: 0.817 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	11.864LeuAla: 11.864 ± 0.084	1.1LeuCys: 1.1 ± 0.023	6.226LeuAsp: 6.226 ± 0.044	5.861LeuGlu: 5.861 ± 0.055	3.234LeuPhe: 3.234 ± 0.038	6.613LeuGly: 6.613 ± 0.045	2.201LeuHis: 2.201 ± 0.027	2.665LeuIle: 2.665 ± 0.036	3.425LeuLys: 3.425 ± 0.037	8.729LeuLeu: 8.729 ± 0.079	1.276LeuMet: 1.276 ± 0.028	2.205LeuAsn: 2.205 ± 0.028	6.44LeuPro: 6.44 ± 0.052	2.836LeuGln: 2.836 ± 0.033	6.439LeuArg: 6.439 ± 0.049	8.034LeuSer: 8.034 ± 0.059	4.217LeuThr: 4.217 ± 0.04	7.68LeuVal: 7.68 ± 0.067	1.074LeuTrp: 1.074 ± 0.021	2.111LeuTyr: 2.111 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	1.755MetAla: 1.755 ± 0.023	0.182MetCys: 0.182 ± 0.007	0.869MetAsp: 0.869 ± 0.016	0.689MetGlu: 0.689 ± 0.02	0.475MetPhe: 0.475 ± 0.014	1.153MetGly: 1.153 ± 0.022	0.385MetHis: 0.385 ± 0.013	0.506MetIle: 0.506 ± 0.013	0.491MetLys: 0.491 ± 0.014	1.48MetLeu: 1.48 ± 0.021	0.322MetMet: 0.322 ± 0.011	0.371MetAsn: 0.371 ± 0.011	1.023MetPro: 1.023 ± 0.017	0.607MetGln: 0.607 ± 0.014	1.206MetArg: 1.206 ± 0.019	1.481MetSer: 1.481 ± 0.023	0.829MetThr: 0.829 ± 0.016	0.93MetVal: 0.93 ± 0.016	0.193MetTrp: 0.193 ± 0.007	0.359MetTyr: 0.359 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.337AsnAla: 2.337 ± 0.028	0.244AsnCys: 0.244 ± 0.009	1.238AsnAsp: 1.238 ± 0.017	1.268AsnGlu: 1.268 ± 0.021	0.785AsnPhe: 0.785 ± 0.018	1.857AsnGly: 1.857 ± 0.029	0.415AsnHis: 0.415 ± 0.011	0.852AsnIle: 0.852 ± 0.02	0.955AsnLys: 0.955 ± 0.019	2.29AsnLeu: 2.29 ± 0.028	0.373AsnMet: 0.373 ± 0.011	0.567AsnAsn: 0.567 ± 0.016	1.643AsnPro: 1.643 ± 0.024	0.64AsnGln: 0.64 ± 0.015	1.207AsnArg: 1.207 ± 0.016	1.499AsnSer: 1.499 ± 0.022	1.047AsnThr: 1.047 ± 0.019	1.638AsnVal: 1.638 ± 0.024	0.315AsnTrp: 0.315 ± 0.01	0.576AsnTyr: 0.576 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.303ProAla: 9.303 ± 0.094	0.452ProCys: 0.452 ± 0.011	3.348ProAsp: 3.348 ± 0.033	2.935ProGlu: 2.935 ± 0.032	2.159ProPhe: 2.159 ± 0.024	4.207ProGly: 4.207 ± 0.04	2.027ProHis: 2.027 ± 0.033	1.726ProIle: 1.726 ± 0.025	1.905ProLys: 1.905 ± 0.027	6.13ProLeu: 6.13 ± 0.046	0.773ProMet: 0.773 ± 0.014	1.317ProAsn: 1.317 ± 0.023	8.816ProPro: 8.816 ± 0.126	2.311ProGln: 2.311 ± 0.036	4.847ProArg: 4.847 ± 0.06	8.848ProSer: 8.848 ± 0.078	4.623ProThr: 4.623 ± 0.045	4.464ProVal: 4.464 ± 0.047	0.606ProTrp: 0.606 ± 0.013	1.395ProTyr: 1.395 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.873GlnAla: 3.873 ± 0.036	0.318GlnCys: 0.318 ± 0.011	1.708GlnAsp: 1.708 ± 0.023	1.686GlnGlu: 1.686 ± 0.024	0.932GlnPhe: 0.932 ± 0.019	2.541GlnGly: 2.541 ± 0.031	1.218GlnHis: 1.218 ± 0.023	1.06GlnIle: 1.06 ± 0.022	1.08GlnLys: 1.08 ± 0.021	3.577GlnLeu: 3.577 ± 0.038	0.584GlnMet: 0.584 ± 0.014	0.694GlnAsn: 0.694 ± 0.012	2.465GlnPro: 2.465 ± 0.038	2.866GlnGln: 2.866 ± 0.08	2.74GlnArg: 2.74 ± 0.032	2.1GlnSer: 2.1 ± 0.03	1.463GlnThr: 1.463 ± 0.026	2.158GlnVal: 2.158 ± 0.03	0.394GlnTrp: 0.394 ± 0.01	0.782GlnTyr: 0.782 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	8.592ArgAla: 8.592 ± 0.078	0.693ArgCys: 0.693 ± 0.014	4.079ArgAsp: 4.079 ± 0.046	4.386ArgGlu: 4.386 ± 0.043	2.125ArgPhe: 2.125 ± 0.025	4.665ArgGly: 4.665 ± 0.052	1.72ArgHis: 1.72 ± 0.026	2.316ArgIle: 2.316 ± 0.025	3.006ArgLys: 3.006 ± 0.037	6.572ArgLeu: 6.572 ± 0.056	1.129ArgMet: 1.129 ± 0.022	1.567ArgAsn: 1.567 ± 0.024	5.033ArgPro: 5.033 ± 0.061	2.492ArgGln: 2.492 ± 0.032	7.918ArgArg: 7.918 ± 0.121	5.578ArgSer: 5.578 ± 0.06	3.937ArgThr: 3.937 ± 0.04	4.304ArgVal: 4.304 ± 0.037	1.015ArgTrp: 1.015 ± 0.016	1.417ArgTyr: 1.417 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	10.17SerAla: 10.17 ± 0.093	0.758SerCys: 0.758 ± 0.018	3.804SerAsp: 3.804 ± 0.036	2.937SerGlu: 2.937 ± 0.03	2.944SerPhe: 2.944 ± 0.033	5.764SerGly: 5.764 ± 0.053	1.926SerHis: 1.926 ± 0.025	2.536SerIle: 2.536 ± 0.03	2.348SerLys: 2.348 ± 0.034	8.035SerLeu: 8.035 ± 0.062	1.196SerMet: 1.196 ± 0.017	1.746SerAsn: 1.746 ± 0.022	7.48SerPro: 7.48 ± 0.084	2.181SerGln: 2.181 ± 0.029	5.73SerArg: 5.73 ± 0.062	13.773SerSer: 13.773 ± 0.144	7.041SerThr: 7.041 ± 0.067	4.368SerVal: 4.368 ± 0.036	0.946SerTrp: 0.946 ± 0.019	1.676SerTyr: 1.676 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	6.489ThrAla: 6.489 ± 0.05	0.63ThrCys: 0.63 ± 0.017	2.427ThrAsp: 2.427 ± 0.027	2.03ThrGlu: 2.03 ± 0.026	1.992ThrPhe: 1.992 ± 0.023	3.696ThrGly: 3.696 ± 0.036	1.147ThrHis: 1.147 ± 0.017	1.884ThrIle: 1.884 ± 0.027	1.837ThrLys: 1.837 ± 0.025	5.435ThrLeu: 5.435 ± 0.051	0.773ThrMet: 0.773 ± 0.014	1.139ThrAsn: 1.139 ± 0.019	4.221ThrPro: 4.221 ± 0.044	1.432ThrGln: 1.432 ± 0.022	3.458ThrArg: 3.458 ± 0.039	6.122ThrSer: 6.122 ± 0.056	3.95ThrThr: 3.95 ± 0.046	3.359ThrVal: 3.359 ± 0.033	0.723ThrTrp: 0.723 ± 0.016	1.214ThrTyr: 1.214 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	7.536ValAla: 7.536 ± 0.053	0.764ValCys: 0.764 ± 0.017	4.637ValAsp: 4.637 ± 0.04	4.678ValGlu: 4.678 ± 0.039	2.199ValPhe: 2.199 ± 0.027	4.892ValGly: 4.892 ± 0.039	1.583ValHis: 1.583 ± 0.022	2.023ValIle: 2.023 ± 0.031	2.896ValLys: 2.896 ± 0.037	6.333ValLeu: 6.333 ± 0.055	0.983ValMet: 0.983 ± 0.018	1.563ValAsn: 1.563 ± 0.028	4.282ValPro: 4.282 ± 0.041	2.273ValGln: 2.273 ± 0.03	4.675ValArg: 4.675 ± 0.046	4.171ValSer: 4.171 ± 0.038	3.087ValThr: 3.087 ± 0.03	5.67ValVal: 5.67 ± 0.046	0.918ValTrp: 0.918 ± 0.021	1.465ValTyr: 1.465 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.317TrpAla: 1.317 ± 0.021	0.169TrpCys: 0.169 ± 0.007	0.833TrpAsp: 0.833 ± 0.016	0.692TrpGlu: 0.692 ± 0.014	0.421TrpPhe: 0.421 ± 0.01	0.778TrpGly: 0.778 ± 0.016	0.306TrpHis: 0.306 ± 0.009	0.501TrpIle: 0.501 ± 0.013	0.462TrpLys: 0.462 ± 0.013	1.236TrpLeu: 1.236 ± 0.022	0.255TrpMet: 0.255 ± 0.008	0.369TrpAsn: 0.369 ± 0.012	0.542TrpPro: 0.542 ± 0.019	0.428TrpGln: 0.428 ± 0.01	1.068TrpArg: 1.068 ± 0.02	0.956TrpSer: 0.956 ± 0.018	0.855TrpThr: 0.855 ± 0.017	0.862TrpVal: 0.862 ± 0.017	0.251TrpTrp: 0.251 ± 0.009	0.315TrpTyr: 0.315 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.247TyrAla: 2.247 ± 0.031	0.297TyrCys: 0.297 ± 0.011	1.394TyrAsp: 1.394 ± 0.019	1.159TyrGlu: 1.159 ± 0.019	0.848TyrPhe: 0.848 ± 0.017	1.801TyrGly: 1.801 ± 0.029	0.536TyrHis: 0.536 ± 0.014	0.728TyrIle: 0.728 ± 0.017	0.765TyrLys: 0.765 ± 0.015	2.327TyrLeu: 2.327 ± 0.028	0.339TyrMet: 0.339 ± 0.011	0.601TyrAsn: 0.601 ± 0.015	1.257TyrPro: 1.257 ± 0.019	0.7TyrGln: 0.7 ± 0.017	1.435TyrArg: 1.435 ± 0.021	1.592TyrSer: 1.592 ± 0.025	1.132TyrThr: 1.132 ± 0.021	1.398TyrVal: 1.398 ± 0.02	0.287TyrTrp: 0.287 ± 0.01	0.619TyrTyr: 0.619 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7228 proteins (3636073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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