Streptococcus cuniculi
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1969 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8EAG3|A0A1Q8EAG3_9STRE DNA replication protein DnaD OS=Streptococcus cuniculi OX=1432788 GN=BU202_00385 PE=4 SV=1
MM1 pKa = 7.7 NITLYY6 pKa = 10.19 PEE8 pKa = 3.91 KK9 pKa = 10.64 CIACGLCQTMSDD21 pKa = 3.55 VFDD24 pKa = 4.63 YY25 pKa = 11.3 DD26 pKa = 3.73 DD27 pKa = 5.04 DD28 pKa = 6.36 GIVLFADD35 pKa = 3.75 DD36 pKa = 4.73 HH37 pKa = 5.91 QASTMTLSDD46 pKa = 3.41 RR47 pKa = 11.84 TDD49 pKa = 3.13 ALLAAKK55 pKa = 9.89 SCPTKK60 pKa = 10.62 AISVDD65 pKa = 3.28
Molecular weight: 7.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.617
ProMoST 3.961
Dawson 3.884
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 4.012
DTASelect 4.329
Thurlkill 3.719
EMBOSS 3.897
Sillero 3.986
Patrickios 0.782
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A1Q8E733|A0A1Q8E733_9STRE Membrane protein insertase YidC OS=Streptococcus cuniculi OX=1432788 GN=yidC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.26 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.78 GRR39 pKa = 11.84 KK40 pKa = 8.38 VLAVAA45 pKa = 4.94
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1969
0
1969
604276
23
2634
306.9
34.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.135 ± 0.066
0.584 ± 0.014
5.6 ± 0.065
7.189 ± 0.067
4.426 ± 0.043
6.727 ± 0.055
2.014 ± 0.027
7.023 ± 0.057
6.146 ± 0.051
10.12 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.571 ± 0.029
3.932 ± 0.033
3.457 ± 0.043
4.241 ± 0.051
4.205 ± 0.046
5.883 ± 0.054
5.785 ± 0.063
7.26 ± 0.057
0.929 ± 0.02
3.775 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here