Amino acid dipepetide frequency for Clostridium puniceum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.886AlaAla: 3.886 ± 0.065
0.714AlaCys: 0.714 ± 0.024
2.767AlaAsp: 2.767 ± 0.047
3.569AlaGlu: 3.569 ± 0.055
2.494AlaPhe: 2.494 ± 0.05
3.697AlaGly: 3.697 ± 0.062
0.795AlaHis: 0.795 ± 0.023
5.611AlaIle: 5.611 ± 0.068
4.736AlaLys: 4.736 ± 0.06
5.538AlaLeu: 5.538 ± 0.06
1.616AlaMet: 1.616 ± 0.032
2.929AlaAsn: 2.929 ± 0.042
1.404AlaPro: 1.404 ± 0.031
1.522AlaGln: 1.522 ± 0.032
1.731AlaArg: 1.731 ± 0.032
3.48AlaSer: 3.48 ± 0.051
3.017AlaThr: 3.017 ± 0.049
3.802AlaVal: 3.802 ± 0.056
0.467AlaTrp: 0.467 ± 0.02
2.152AlaTyr: 2.152 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.607CysAla: 0.607 ± 0.021
0.189CysCys: 0.189 ± 0.013
0.696CysAsp: 0.696 ± 0.02
0.796CysGlu: 0.796 ± 0.022
0.54CysPhe: 0.54 ± 0.02
1.042CysGly: 1.042 ± 0.029
0.229CysHis: 0.229 ± 0.011
1.191CysIle: 1.191 ± 0.033
0.979CysLys: 0.979 ± 0.025
0.898CysLeu: 0.898 ± 0.026
0.298CysMet: 0.298 ± 0.015
0.804CysAsn: 0.804 ± 0.024
0.459CysPro: 0.459 ± 0.02
0.232CysGln: 0.232 ± 0.011
0.392CysArg: 0.392 ± 0.016
0.787CysSer: 0.787 ± 0.023
0.627CysThr: 0.627 ± 0.021
0.648CysVal: 0.648 ± 0.02
0.088CysTrp: 0.088 ± 0.008
0.497CysTyr: 0.497 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.679AspAla: 2.679 ± 0.051
0.629AspCys: 0.629 ± 0.019
2.806AspAsp: 2.806 ± 0.049
4.633AspGlu: 4.633 ± 0.055
2.787AspPhe: 2.787 ± 0.043
3.691AspGly: 3.691 ± 0.057
0.548AspHis: 0.548 ± 0.019
5.807AspIle: 5.807 ± 0.066
5.198AspLys: 5.198 ± 0.057
4.83AspLeu: 4.83 ± 0.056
1.452AspMet: 1.452 ± 0.031
3.533AspAsn: 3.533 ± 0.052
1.292AspPro: 1.292 ± 0.029
0.927AspGln: 0.927 ± 0.023
1.679AspArg: 1.679 ± 0.035
3.32AspSer: 3.32 ± 0.051
2.602AspThr: 2.602 ± 0.039
3.371AspVal: 3.371 ± 0.051
0.483AspTrp: 0.483 ± 0.018
2.737AspTyr: 2.737 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
4.291GluAla: 4.291 ± 0.06
0.79GluCys: 0.79 ± 0.025
4.179GluAsp: 4.179 ± 0.057
6.89GluGlu: 6.89 ± 0.086
3.181GluPhe: 3.181 ± 0.049
3.962GluGly: 3.962 ± 0.062
1.01GluHis: 1.01 ± 0.032
7.622GluIle: 7.622 ± 0.071
7.665GluLys: 7.665 ± 0.096
6.923GluLeu: 6.923 ± 0.076
1.926GluMet: 1.926 ± 0.036
5.558GluAsn: 5.558 ± 0.064
1.394GluPro: 1.394 ± 0.028
2.016GluGln: 2.016 ± 0.037
2.594GluArg: 2.594 ± 0.046
3.638GluSer: 3.638 ± 0.053
3.072GluThr: 3.072 ± 0.041
4.754GluVal: 4.754 ± 0.062
0.578GluTrp: 0.578 ± 0.019
3.198GluTyr: 3.198 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
2.261PheAla: 2.261 ± 0.04
0.556PheCys: 0.556 ± 0.018
2.622PheAsp: 2.622 ± 0.043
2.998PheGlu: 2.998 ± 0.043
1.969PhePhe: 1.969 ± 0.042
2.808PheGly: 2.808 ± 0.051
0.614PheHis: 0.614 ± 0.02
4.438PheIle: 4.438 ± 0.066
3.933PheLys: 3.933 ± 0.047
3.676PheLeu: 3.676 ± 0.057
1.091PheMet: 1.091 ± 0.026
3.072PheAsn: 3.072 ± 0.051
1.122PhePro: 1.122 ± 0.026
1.006PheGln: 1.006 ± 0.027
1.27PheArg: 1.27 ± 0.026
3.136PheSer: 3.136 ± 0.051
2.351PheThr: 2.351 ± 0.036
2.535PheVal: 2.535 ± 0.041
0.346PheTrp: 0.346 ± 0.014
1.831PheTyr: 1.831 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
4.017GlyAla: 4.017 ± 0.065
0.907GlyCys: 0.907 ± 0.027
3.221GlyAsp: 3.221 ± 0.041
4.201GlyGlu: 4.201 ± 0.055
2.966GlyPhe: 2.966 ± 0.038
4.161GlyGly: 4.161 ± 0.067
0.972GlyHis: 0.972 ± 0.027
6.716GlyIle: 6.716 ± 0.081
5.328GlyLys: 5.328 ± 0.062
5.132GlyLeu: 5.132 ± 0.072
1.729GlyMet: 1.729 ± 0.037
3.534GlyAsn: 3.534 ± 0.055
1.146GlyPro: 1.146 ± 0.038
1.538GlyGln: 1.538 ± 0.033
1.992GlyArg: 1.992 ± 0.038
3.679GlySer: 3.679 ± 0.067
3.578GlyThr: 3.578 ± 0.056
4.276GlyVal: 4.276 ± 0.063
0.707GlyTrp: 0.707 ± 0.026
3.04GlyTyr: 3.04 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
0.697HisAla: 0.697 ± 0.02
0.223HisCys: 0.223 ± 0.012
0.72HisAsp: 0.72 ± 0.022
0.963HisGlu: 0.963 ± 0.025
0.667HisPhe: 0.667 ± 0.018
0.995HisGly: 0.995 ± 0.027
0.291HisHis: 0.291 ± 0.015
1.293HisIle: 1.293 ± 0.03
1.086HisLys: 1.086 ± 0.025
1.18HisLeu: 1.18 ± 0.034
0.356HisMet: 0.356 ± 0.015
0.846HisAsn: 0.846 ± 0.023
0.55HisPro: 0.55 ± 0.019
0.331HisGln: 0.331 ± 0.016
0.487HisArg: 0.487 ± 0.017
0.868HisSer: 0.868 ± 0.022
0.678HisThr: 0.678 ± 0.022
0.736HisVal: 0.736 ± 0.022
0.14HisTrp: 0.14 ± 0.009
0.62HisTyr: 0.62 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
5.672IleAla: 5.672 ± 0.057
1.293IleCys: 1.293 ± 0.03
5.739IleAsp: 5.739 ± 0.068
7.358IleGlu: 7.358 ± 0.08
4.107IlePhe: 4.107 ± 0.064
6.054IleGly: 6.054 ± 0.083
1.293IleHis: 1.293 ± 0.031
9.49IleIle: 9.49 ± 0.097
8.997IleLys: 8.997 ± 0.084
9.017IleLeu: 9.017 ± 0.09
2.299IleMet: 2.299 ± 0.041
6.834IleAsn: 6.834 ± 0.072
3.336IlePro: 3.336 ± 0.05
2.447IleGln: 2.447 ± 0.039
2.913IleArg: 2.913 ± 0.045
7.248IleSer: 7.248 ± 0.077
5.184IleThr: 5.184 ± 0.059
5.782IleVal: 5.782 ± 0.07
0.589IleTrp: 0.589 ± 0.019
3.664IleTyr: 3.664 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.955LysAla: 4.955 ± 0.059
0.94LysCys: 0.94 ± 0.028
5.654LysAsp: 5.654 ± 0.064
8.771LysGlu: 8.771 ± 0.1
3.323LysPhe: 3.323 ± 0.051
4.824LysGly: 4.824 ± 0.064
1.135LysHis: 1.135 ± 0.03
8.553LysIle: 8.553 ± 0.081
7.967LysLys: 7.967 ± 0.077
7.812LysLeu: 7.812 ± 0.077
2.319LysMet: 2.319 ± 0.043
6.518LysAsn: 6.518 ± 0.071
2.04LysPro: 2.04 ± 0.035
2.447LysGln: 2.447 ± 0.047
2.853LysArg: 2.853 ± 0.038
5.304LysSer: 5.304 ± 0.06
4.227LysThr: 4.227 ± 0.053
5.699LysVal: 5.699 ± 0.069
0.737LysTrp: 0.737 ± 0.022
4.189LysTyr: 4.189 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
4.977LeuAla: 4.977 ± 0.062
1.068LeuCys: 1.068 ± 0.026
5.047LeuAsp: 5.047 ± 0.063
6.557LeuGlu: 6.557 ± 0.091
3.668LeuPhe: 3.668 ± 0.055
5.761LeuGly: 5.761 ± 0.068
1.108LeuHis: 1.108 ± 0.022
8.209LeuIle: 8.209 ± 0.09
8.726LeuLys: 8.726 ± 0.084
7.596LeuLeu: 7.596 ± 0.092
2.252LeuMet: 2.252 ± 0.035
6.148LeuAsn: 6.148 ± 0.07
2.685LeuPro: 2.685 ± 0.046
2.249LeuGln: 2.249 ± 0.045
2.923LeuArg: 2.923 ± 0.045
6.478LeuSer: 6.478 ± 0.059
4.569LeuThr: 4.569 ± 0.054
4.97LeuVal: 4.97 ± 0.064
0.623LeuTrp: 0.623 ± 0.021
3.265LeuTyr: 3.265 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
1.637MetAla: 1.637 ± 0.036
0.265MetCys: 0.265 ± 0.012
1.363MetAsp: 1.363 ± 0.028
1.817MetGlu: 1.817 ± 0.033
1.018MetPhe: 1.018 ± 0.028
1.656MetGly: 1.656 ± 0.034
0.373MetHis: 0.373 ± 0.016
2.311MetIle: 2.311 ± 0.037
2.533MetLys: 2.533 ± 0.042
2.224MetLeu: 2.224 ± 0.036
0.624MetMet: 0.624 ± 0.022
1.786MetAsn: 1.786 ± 0.034
0.895MetPro: 0.895 ± 0.023
0.722MetGln: 0.722 ± 0.022
0.819MetArg: 0.819 ± 0.022
1.682MetSer: 1.682 ± 0.033
1.215MetThr: 1.215 ± 0.031
1.502MetVal: 1.502 ± 0.03
0.161MetTrp: 0.161 ± 0.011
0.863MetTyr: 0.863 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.076AsnAla: 3.076 ± 0.051
0.746AsnCys: 0.746 ± 0.025
3.322AsnAsp: 3.322 ± 0.047
4.845AsnGlu: 4.845 ± 0.057
2.818AsnPhe: 2.818 ± 0.044
4.101AsnGly: 4.101 ± 0.055
0.895AsnHis: 0.895 ± 0.021
7.195AsnIle: 7.195 ± 0.068
6.31AsnLys: 6.31 ± 0.071
6.001AsnLeu: 6.001 ± 0.065
1.734AsnMet: 1.734 ± 0.035
4.896AsnAsn: 4.896 ± 0.075
2.112AsnPro: 2.112 ± 0.037
1.652AsnGln: 1.652 ± 0.033
1.985AsnArg: 1.985 ± 0.038
4.541AsnSer: 4.541 ± 0.065
3.208AsnThr: 3.208 ± 0.045
3.757AsnVal: 3.757 ± 0.054
0.547AsnTrp: 0.547 ± 0.019
2.922AsnTyr: 2.922 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
1.435ProAla: 1.435 ± 0.034
0.307ProCys: 0.307 ± 0.013
1.376ProAsp: 1.376 ± 0.031
2.028ProGlu: 2.028 ± 0.04
1.366ProPhe: 1.366 ± 0.03
1.491ProGly: 1.491 ± 0.038
0.45ProHis: 0.45 ± 0.018
2.734ProIle: 2.734 ± 0.039
2.213ProLys: 2.213 ± 0.04
2.369ProLeu: 2.369 ± 0.038
0.683ProMet: 0.683 ± 0.019
1.694ProAsn: 1.694 ± 0.033
0.599ProPro: 0.599 ± 0.022
0.823ProGln: 0.823 ± 0.023
0.761ProArg: 0.761 ± 0.024
1.792ProSer: 1.792 ± 0.03
1.473ProThr: 1.473 ± 0.035
1.901ProVal: 1.901 ± 0.034
0.268ProTrp: 0.268 ± 0.014
1.245ProTyr: 1.245 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
1.439GlnAla: 1.439 ± 0.033
0.27GlnCys: 0.27 ± 0.014
1.324GlnAsp: 1.324 ± 0.026
1.886GlnGlu: 1.886 ± 0.038
1.075GlnPhe: 1.075 ± 0.027
1.74GlnGly: 1.74 ± 0.104
0.317GlnHis: 0.317 ± 0.015
2.405GlnIle: 2.405 ± 0.037
2.29GlnLys: 2.29 ± 0.04
2.236GlnLeu: 2.236 ± 0.037
0.699GlnMet: 0.699 ± 0.023
1.686GlnAsn: 1.686 ± 0.035
0.533GlnPro: 0.533 ± 0.019
0.73GlnGln: 0.73 ± 0.021
0.898GlnArg: 0.898 ± 0.026
1.469GlnSer: 1.469 ± 0.033
1.232GlnThr: 1.232 ± 0.03
1.571GlnVal: 1.571 ± 0.032
0.263GlnTrp: 0.263 ± 0.012
1.156GlnTyr: 1.156 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
1.689ArgAla: 1.689 ± 0.037
0.404ArgCys: 0.404 ± 0.015
1.666ArgAsp: 1.666 ± 0.034
2.617ArgGlu: 2.617 ± 0.048
1.397ArgPhe: 1.397 ± 0.031
1.783ArgGly: 1.783 ± 0.035
0.445ArgHis: 0.445 ± 0.018
3.199ArgIle: 3.199 ± 0.049
2.941ArgLys: 2.941 ± 0.042
2.768ArgLeu: 2.768 ± 0.044
0.85ArgMet: 0.85 ± 0.024
2.069ArgAsn: 2.069 ± 0.037
0.784ArgPro: 0.784 ± 0.022
0.83ArgGln: 0.83 ± 0.019
1.248ArgArg: 1.248 ± 0.029
1.597ArgSer: 1.597 ± 0.029
1.596ArgThr: 1.596 ± 0.033
1.954ArgVal: 1.954 ± 0.038
0.292ArgTrp: 0.292 ± 0.014
1.404ArgTyr: 1.404 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
3.48SerAla: 3.48 ± 0.048
0.66SerCys: 0.66 ± 0.02
3.434SerAsp: 3.434 ± 0.052
4.377SerGlu: 4.377 ± 0.06
2.981SerPhe: 2.981 ± 0.043
4.245SerGly: 4.245 ± 0.053
0.921SerHis: 0.921 ± 0.027
6.683SerIle: 6.683 ± 0.076
5.813SerLys: 5.813 ± 0.068
5.875SerLeu: 5.875 ± 0.068
1.635SerMet: 1.635 ± 0.031
4.211SerAsn: 4.211 ± 0.063
1.583SerPro: 1.583 ± 0.03
1.747SerGln: 1.747 ± 0.052
1.971SerArg: 1.971 ± 0.046
4.515SerSer: 4.515 ± 0.059
3.411SerThr: 3.411 ± 0.053
3.778SerVal: 3.778 ± 0.056
0.538SerTrp: 0.538 ± 0.021
2.737SerTyr: 2.737 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
3.079ThrAla: 3.079 ± 0.054
0.54ThrCys: 0.54 ± 0.019
2.569ThrAsp: 2.569 ± 0.043
3.156ThrGlu: 3.156 ± 0.043
2.24ThrPhe: 2.24 ± 0.035
3.767ThrGly: 3.767 ± 0.05
0.772ThrHis: 0.772 ± 0.022
5.08ThrIle: 5.08 ± 0.056
3.888ThrLys: 3.888 ± 0.054
4.822ThrLeu: 4.822 ± 0.054
1.148ThrMet: 1.148 ± 0.027
3.129ThrAsn: 3.129 ± 0.053
1.762ThrPro: 1.762 ± 0.033
1.282ThrGln: 1.282 ± 0.033
1.44ThrArg: 1.44 ± 0.027
3.433ThrSer: 3.433 ± 0.057
2.954ThrThr: 2.954 ± 0.057
3.264ThrVal: 3.264 ± 0.039
0.428ThrTrp: 0.428 ± 0.019
2.069ThrTyr: 2.069 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
3.638ValAla: 3.638 ± 0.049
0.801ValCys: 0.801 ± 0.021
3.546ValAsp: 3.546 ± 0.051
4.17ValGlu: 4.17 ± 0.059
2.64ValPhe: 2.64 ± 0.039
3.886ValGly: 3.886 ± 0.054
0.793ValHis: 0.793 ± 0.022
5.986ValIle: 5.986 ± 0.07
5.244ValLys: 5.244 ± 0.061
5.449ValLeu: 5.449 ± 0.06
1.525ValMet: 1.525 ± 0.03
3.822ValAsn: 3.822 ± 0.047
1.865ValPro: 1.865 ± 0.035
1.524ValGln: 1.524 ± 0.033
1.829ValArg: 1.829 ± 0.035
4.225ValSer: 4.225 ± 0.052
3.386ValThr: 3.386 ± 0.053
4.068ValVal: 4.068 ± 0.054
0.429ValTrp: 0.429 ± 0.014
2.365ValTyr: 2.365 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
0.448TrpAla: 0.448 ± 0.018
0.128TrpCys: 0.128 ± 0.007
0.44TrpAsp: 0.44 ± 0.017
0.487TrpGlu: 0.487 ± 0.02
0.394TrpPhe: 0.394 ± 0.017
0.571TrpGly: 0.571 ± 0.021
0.156TrpHis: 0.156 ± 0.011
0.78TrpIle: 0.78 ± 0.025
0.627TrpLys: 0.627 ± 0.02
0.667TrpLeu: 0.667 ± 0.024
0.235TrpMet: 0.235 ± 0.012
0.605TrpAsn: 0.605 ± 0.022
0.185TrpPro: 0.185 ± 0.012
0.278TrpGln: 0.278 ± 0.012
0.28TrpArg: 0.28 ± 0.013
0.534TrpSer: 0.534 ± 0.02
0.401TrpThr: 0.401 ± 0.015
0.412TrpVal: 0.412 ± 0.016
0.111TrpTrp: 0.111 ± 0.008
0.439TrpTyr: 0.439 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.963TyrAla: 1.963 ± 0.039
0.568TyrCys: 0.568 ± 0.021
2.513TyrAsp: 2.513 ± 0.046
3.119TyrGlu: 3.119 ± 0.049
2.006TyrPhe: 2.006 ± 0.038
2.602TyrGly: 2.602 ± 0.04
0.607TyrHis: 0.607 ± 0.021
4.013TyrIle: 4.013 ± 0.059
3.793TyrLys: 3.793 ± 0.056
3.866TyrLeu: 3.866 ± 0.061
0.999TyrMet: 0.999 ± 0.027
2.99TyrAsn: 2.99 ± 0.043
1.262TyrPro: 1.262 ± 0.029
0.914TyrGln: 0.914 ± 0.024
1.471TyrArg: 1.471 ± 0.03
2.87TyrSer: 2.87 ± 0.048
2.043TyrThr: 2.043 ± 0.04
2.413TyrVal: 2.413 ± 0.036
0.38TyrTrp: 0.38 ± 0.017
2.093TyrTyr: 2.093 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5300 proteins (1617317 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski