Fusarium solani virus 1
Average proteome isoelectric point is 8.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83329|Q83329_9VIRU Capsid protein OS=Fusarium solani virus 1 OX=1511847 PE=4 SV=1
MM1 pKa = 7.35 SSSVAPGDD9 pKa = 3.93 FVSSAGSGKK18 pKa = 10.34 KK19 pKa = 9.59 NRR21 pKa = 11.84 PGKK24 pKa = 10.13 KK25 pKa = 8.58 EE26 pKa = 3.6 RR27 pKa = 11.84 QARR30 pKa = 11.84 RR31 pKa = 11.84 GLSEE35 pKa = 3.89 ATASNAPSSLTGAAVFAAGAPADD58 pKa = 4.22 PVPQPGRR65 pKa = 11.84 YY66 pKa = 8.34 PVVFPSGAGEE76 pKa = 3.94 PTRR79 pKa = 11.84 DD80 pKa = 3.41 SLFAYY85 pKa = 10.03 DD86 pKa = 5.01 GEE88 pKa = 4.92 SIQDD92 pKa = 3.73 TVSEE96 pKa = 4.14 LTDD99 pKa = 3.72 RR100 pKa = 11.84 YY101 pKa = 9.68 VQNSKK106 pKa = 10.08 YY107 pKa = 10.93 AEE109 pKa = 4.1 FAAHH113 pKa = 6.51 SGLNFGDD120 pKa = 4.61 LEE122 pKa = 4.29 TLILRR127 pKa = 11.84 GIFLGLAQQTVHH139 pKa = 5.59 SHH141 pKa = 4.75 VNMGLPMGDD150 pKa = 4.41 FSPVATSDD158 pKa = 3.55 VVNFAAIRR166 pKa = 11.84 SIISQFGEE174 pKa = 4.03 FQSVSEE180 pKa = 4.16 GTRR183 pKa = 11.84 YY184 pKa = 10.34 LLAGYY189 pKa = 8.7 EE190 pKa = 4.33 STVAACVRR198 pKa = 11.84 AAKK201 pKa = 10.22 RR202 pKa = 11.84 ADD204 pKa = 3.27 SDD206 pKa = 3.71 NPKK209 pKa = 10.34 RR210 pKa = 11.84 LAQTFWLPTRR220 pKa = 11.84 VDD222 pKa = 3.67 DD223 pKa = 4.59 QRR225 pKa = 11.84 TKK227 pKa = 11.13 YY228 pKa = 10.28 VVAHH232 pKa = 7.0 KK233 pKa = 9.97 LASYY237 pKa = 8.22 CIPFGVYY244 pKa = 10.4 LDD246 pKa = 3.99 VEE248 pKa = 4.57 EE249 pKa = 4.77 LAHH252 pKa = 6.54 HH253 pKa = 6.3 VFSSSSDD260 pKa = 2.66 AWDD263 pKa = 3.33 AVKK266 pKa = 10.9 VLLGADD272 pKa = 3.16 AAAQNRR278 pKa = 11.84 FDD280 pKa = 5.1 FLFGTYY286 pKa = 7.66 NTEE289 pKa = 3.73 AAFLSLVTGSADD301 pKa = 3.51 RR302 pKa = 11.84 VDD304 pKa = 2.96 WLARR308 pKa = 11.84 VRR310 pKa = 11.84 NTLAKK315 pKa = 10.13 FFSLGSGLQNRR326 pKa = 11.84 AAASGSLGQMSRR338 pKa = 11.84 VVDD341 pKa = 3.52 MSGVSLVSSRR351 pKa = 11.84 LAVSRR356 pKa = 11.84 PGILTACVFPVLWSVFRR373 pKa = 11.84 PDD375 pKa = 3.62 PVSCGVNHH383 pKa = 5.9 FVKK386 pKa = 10.78 YY387 pKa = 10.64 GSTSYY392 pKa = 11.19 GVYY395 pKa = 6.0 TTRR398 pKa = 11.84 LAVISDD404 pKa = 3.38 TLNRR408 pKa = 11.84 VWAGLL413 pKa = 3.51
Molecular weight: 44.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.086
IPC2_protein 7.395
IPC_protein 7.468
Toseland 7.205
ProMoST 7.878
Dawson 8.068
Bjellqvist 8.273
Wikipedia 8.009
Rodwell 8.053
Grimsley 7.263
Solomon 8.2
Lehninger 8.214
Nozaki 8.39
DTASelect 8.141
Thurlkill 8.185
EMBOSS 8.302
Sillero 8.463
Patrickios 4.368
IPC_peptide 8.2
IPC2_peptide 7.483
IPC2.peptide.svr19 7.527
Protein with the highest isoelectric point:
>tr|Q83329|Q83329_9VIRU Capsid protein OS=Fusarium solani virus 1 OX=1511847 PE=4 SV=1
MM1 pKa = 7.38 VEE3 pKa = 4.51 LFVMVDD9 pKa = 3.25 PTTKK13 pKa = 9.86 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 IQSTLGPFLSVPGLQEE32 pKa = 3.32 IARR35 pKa = 11.84 YY36 pKa = 9.38 GGYY39 pKa = 8.47 ATYY42 pKa = 10.46 RR43 pKa = 11.84 AVRR46 pKa = 11.84 NTDD49 pKa = 2.47 PWIRR53 pKa = 11.84 QSLKK57 pKa = 10.91 LFDD60 pKa = 5.26 PDD62 pKa = 4.1 LYY64 pKa = 11.4 GNIYY68 pKa = 10.65 GFTRR72 pKa = 11.84 RR73 pKa = 11.84 PAGPEE78 pKa = 3.73 GMYY81 pKa = 10.46 KK82 pKa = 10.47 SLMKK86 pKa = 10.49 FGEE89 pKa = 4.29 SMPRR93 pKa = 11.84 FTDD96 pKa = 3.02 MSTVQRR102 pKa = 11.84 SAMKK106 pKa = 9.21 TAITAARR113 pKa = 11.84 KK114 pKa = 9.44 RR115 pKa = 11.84 FKK117 pKa = 10.55 TPVKK121 pKa = 10.38 FEE123 pKa = 4.04 PLEE126 pKa = 3.92 WSEE129 pKa = 4.36 VGQHH133 pKa = 5.25 MRR135 pKa = 11.84 RR136 pKa = 11.84 DD137 pKa = 3.61 TSAGVSFPGKK147 pKa = 10.16 KK148 pKa = 9.88 KK149 pKa = 9.79 GDD151 pKa = 3.49 VMEE154 pKa = 5.42 RR155 pKa = 11.84 IYY157 pKa = 11.41 AEE159 pKa = 4.18 GRR161 pKa = 11.84 WLGHH165 pKa = 5.96 RR166 pKa = 11.84 MKK168 pKa = 10.68 QGGKK172 pKa = 10.34 GRR174 pKa = 11.84 FDD176 pKa = 3.39 PRR178 pKa = 11.84 KK179 pKa = 10.23 VRR181 pKa = 11.84 MPPCLATQRR190 pKa = 11.84 GHH192 pKa = 7.6 LSPRR196 pKa = 11.84 DD197 pKa = 3.71 DD198 pKa = 3.96 PKK200 pKa = 11.29 TRR202 pKa = 11.84 LAWIYY207 pKa = 9.27 PSEE210 pKa = 3.9 MLMVEE215 pKa = 4.72 GLYY218 pKa = 10.87 APTMYY223 pKa = 10.58 KK224 pKa = 10.4 AFEE227 pKa = 4.39 AMPDD231 pKa = 3.54 SPLLLGKK238 pKa = 10.34 GSHH241 pKa = 6.42 RR242 pKa = 11.84 LFSEE246 pKa = 4.22 WVSAATPGMRR256 pKa = 11.84 LYY258 pKa = 11.24 GLDD261 pKa = 3.57 FSSFDD266 pKa = 3.49 TKK268 pKa = 10.74 VPAWLIHH275 pKa = 4.74 TAFDD279 pKa = 3.75 ILHH282 pKa = 7.15 DD283 pKa = 5.01 NIDD286 pKa = 3.49 WLHH289 pKa = 5.78 WRR291 pKa = 11.84 GKK293 pKa = 7.93 PTTKK297 pKa = 10.13 RR298 pKa = 11.84 SRR300 pKa = 11.84 QKK302 pKa = 9.82 WKK304 pKa = 10.66 NVWDD308 pKa = 3.64 GMVYY312 pKa = 10.18 YY313 pKa = 9.67 FINTPILMPDD323 pKa = 2.36 GRR325 pKa = 11.84 MFRR328 pKa = 11.84 KK329 pKa = 9.73 RR330 pKa = 11.84 RR331 pKa = 11.84 GVPSGSWWTQLVDD344 pKa = 5.16 SVVNWILVKK353 pKa = 10.78 YY354 pKa = 10.75 LSLCQGVNAKK364 pKa = 8.77 NLRR367 pKa = 11.84 VLGDD371 pKa = 3.88 DD372 pKa = 3.83 SAFMAAEE379 pKa = 4.05 TMDD382 pKa = 4.02 LSVAAEE388 pKa = 3.92 DD389 pKa = 3.72 AAAVGMDD396 pKa = 4.0 LSDD399 pKa = 3.59 EE400 pKa = 4.17 KK401 pKa = 11.27 SISVEE406 pKa = 3.84 DD407 pKa = 3.62 ATEE410 pKa = 4.05 LKK412 pKa = 10.82 LLGVRR417 pKa = 11.84 YY418 pKa = 9.59 RR419 pKa = 11.84 DD420 pKa = 3.08 GHH422 pKa = 5.7 AFRR425 pKa = 11.84 EE426 pKa = 4.28 TEE428 pKa = 3.86 EE429 pKa = 4.1 WFKK432 pKa = 11.12 LALYY436 pKa = 10.03 PEE438 pKa = 4.97 GDD440 pKa = 3.8 VPDD443 pKa = 3.88 IATSLTRR450 pKa = 11.84 LVGLWIGGAMWDD462 pKa = 3.7 TKK464 pKa = 10.32 FSRR467 pKa = 11.84 FMEE470 pKa = 4.39 YY471 pKa = 10.42 FQGCYY476 pKa = 9.37 PCPSEE481 pKa = 4.33 GWFSKK486 pKa = 9.82 DD487 pKa = 2.88 QRR489 pKa = 11.84 RR490 pKa = 11.84 WMEE493 pKa = 3.71 IVHH496 pKa = 6.69 GGRR499 pKa = 11.84 APRR502 pKa = 11.84 GWTKK506 pKa = 10.95 NKK508 pKa = 9.89 NLFWRR513 pKa = 11.84 SIFYY517 pKa = 9.56 TLL519 pKa = 4.4
Molecular weight: 59.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.926
IPC2_protein 9.048
IPC_protein 9.019
Toseland 9.677
ProMoST 9.443
Dawson 9.94
Bjellqvist 9.648
Wikipedia 10.131
Rodwell 10.233
Grimsley 10.028
Solomon 9.97
Lehninger 9.926
Nozaki 9.721
DTASelect 9.619
Thurlkill 9.78
EMBOSS 10.101
Sillero 9.867
Patrickios 5.461
IPC_peptide 9.955
IPC2_peptide 8.229
IPC2.peptide.svr19 8.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
932
413
519
466.0
51.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.906 ± 1.811
0.858 ± 0.067
5.794 ± 0.138
4.077 ± 0.569
5.258 ± 0.191
8.262 ± 0.018
1.717 ± 0.013
2.897 ± 0.439
4.721 ± 1.111
8.369 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.219 ± 1.229
2.468 ± 0.416
5.365 ± 0.468
2.682 ± 0.285
7.511 ± 0.744
8.584 ± 1.712
5.579 ± 0.155
7.618 ± 1.414
2.682 ± 0.901
3.433 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here