Clostridium sp. JN-9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410PRS2|A0A410PRS2_9CLOT Transposase OS=Clostridium sp. JN-9 OX=2507159 GN=EQM05_05050 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 9.83EE3 pKa = 4.06KK4 pKa = 10.66KK5 pKa = 10.44CIFDD9 pKa = 4.6DD10 pKa = 4.53NKK12 pKa = 11.15SCDD15 pKa = 3.68DD16 pKa = 4.13CGKK19 pKa = 10.66CDD21 pKa = 4.96ICDD24 pKa = 4.15LDD26 pKa = 4.51PNKK29 pKa = 10.3ICNNCGKK36 pKa = 10.01CLEE39 pKa = 4.37EE40 pKa = 3.74QWIDD44 pKa = 3.31TKK46 pKa = 10.95AIKK49 pKa = 9.58IDD51 pKa = 4.21EE52 pKa = 4.75IIEE55 pKa = 4.05DD56 pKa = 3.72EE57 pKa = 4.54KK58 pKa = 11.49YY59 pKa = 10.77ADD61 pKa = 4.36KK62 pKa = 10.74IQHH65 pKa = 6.26EE66 pKa = 4.43EE67 pKa = 4.08TNIEE71 pKa = 3.89EE72 pKa = 4.46HH73 pKa = 6.79HH74 pKa = 7.55DD75 pKa = 3.76EE76 pKa = 4.7DD77 pKa = 6.1CSDD80 pKa = 4.21DD81 pKa = 4.51EE82 pKa = 4.89YY83 pKa = 11.87SEE85 pKa = 5.51DD86 pKa = 3.78DD87 pKa = 4.27CSDD90 pKa = 4.2DD91 pKa = 4.96EE92 pKa = 4.99YY93 pKa = 11.8LDD95 pKa = 5.09DD96 pKa = 6.03DD97 pKa = 4.97YY98 pKa = 11.72STEE101 pKa = 4.1EE102 pKa = 4.41HH103 pKa = 7.19DD104 pKa = 4.84SNYY107 pKa = 10.67NNDD110 pKa = 3.94DD111 pKa = 3.81YY112 pKa = 11.97VDD114 pKa = 3.69VMDD117 pKa = 4.84MNVEE121 pKa = 4.78FIDD124 pKa = 4.04DD125 pKa = 3.87VNGLGEE131 pKa = 4.17VLEE134 pKa = 5.5DD135 pKa = 3.55NTKK138 pKa = 10.53LDD140 pKa = 3.84SLFEE144 pKa = 4.09EE145 pKa = 4.66QFPGLIKK152 pKa = 10.77LKK154 pKa = 9.93PSKK157 pKa = 10.75YY158 pKa = 10.31KK159 pKa = 10.71NN160 pKa = 3.22

Molecular weight: 18.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410PRH7|A0A410PRH7_9CLOT CopG family transcriptional regulator OS=Clostridium sp. JN-9 OX=2507159 GN=EQM05_15245 PE=4 SV=1
MM1 pKa = 7.6ARR3 pKa = 11.84EE4 pKa = 3.73EE5 pKa = 4.25RR6 pKa = 11.84NNKK9 pKa = 9.51RR10 pKa = 11.84GSARR14 pKa = 11.84MRR16 pKa = 11.84RR17 pKa = 11.84AKK19 pKa = 10.51KK20 pKa = 9.77KK21 pKa = 9.34VCAFCVDD28 pKa = 3.31KK29 pKa = 11.49AEE31 pKa = 4.67SIDD34 pKa = 3.59YY35 pKa = 10.92KK36 pKa = 10.95DD37 pKa = 3.05INKK40 pKa = 8.67IRR42 pKa = 11.84KK43 pKa = 8.58YY44 pKa = 7.6VTEE47 pKa = 4.3RR48 pKa = 11.84GKK50 pKa = 10.15ILPRR54 pKa = 11.84RR55 pKa = 11.84ISGNCAKK62 pKa = 9.64HH63 pKa = 5.7QRR65 pKa = 11.84MLTEE69 pKa = 3.76AVKK72 pKa = 10.32RR73 pKa = 11.84ARR75 pKa = 11.84NIALLPFTTEE85 pKa = 3.59

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2864

0

2864

876206

23

2877

305.9

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.934 ± 0.044

1.249 ± 0.016

5.573 ± 0.037

6.646 ± 0.05

4.318 ± 0.034

6.37 ± 0.043

1.393 ± 0.015

9.888 ± 0.051

9.191 ± 0.047

8.834 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.021

6.496 ± 0.045

2.898 ± 0.023

2.512 ± 0.021

3.368 ± 0.029

6.502 ± 0.035

4.876 ± 0.03

6.384 ± 0.033

0.69 ± 0.016

4.151 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski