Amino acid dipepetide frequency for Clostridium sp. JN-9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.084AlaAla: 5.084 ± 0.105	0.725AlaCys: 0.725 ± 0.031	3.218AlaAsp: 3.218 ± 0.064	3.853AlaGlu: 3.853 ± 0.076	2.603AlaPhe: 2.603 ± 0.053	4.231AlaGly: 4.231 ± 0.1	0.855AlaHis: 0.855 ± 0.025	5.2AlaIle: 5.2 ± 0.09	4.693AlaLys: 4.693 ± 0.086	5.996AlaLeu: 5.996 ± 0.1	1.775AlaMet: 1.775 ± 0.048	2.533AlaAsn: 2.533 ± 0.053	1.448AlaPro: 1.448 ± 0.044	1.502AlaGln: 1.502 ± 0.038	1.88AlaArg: 1.88 ± 0.05	3.557AlaSer: 3.557 ± 0.073	2.271AlaThr: 2.271 ± 0.057	5.301AlaVal: 5.301 ± 0.096	0.377AlaTrp: 0.377 ± 0.023	2.233AlaTyr: 2.233 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.648CysAla: 0.648 ± 0.03	0.221CysCys: 0.221 ± 0.019	0.751CysAsp: 0.751 ± 0.029	0.754CysGlu: 0.754 ± 0.034	0.543CysPhe: 0.543 ± 0.025	1.114CysGly: 1.114 ± 0.041	0.227CysHis: 0.227 ± 0.017	1.266CysIle: 1.266 ± 0.04	1.017CysLys: 1.017 ± 0.038	0.962CysLeu: 0.962 ± 0.035	0.29CysMet: 0.29 ± 0.017	0.724CysAsn: 0.724 ± 0.03	0.474CysPro: 0.474 ± 0.028	0.252CysGln: 0.252 ± 0.017	0.479CysArg: 0.479 ± 0.025	0.984CysSer: 0.984 ± 0.037	0.623CysThr: 0.623 ± 0.027	0.629CysVal: 0.629 ± 0.031	0.065CysTrp: 0.065 ± 0.011	0.463CysTyr: 0.463 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	2.887AspAla: 2.887 ± 0.065	0.653AspCys: 0.653 ± 0.03	2.78AspAsp: 2.78 ± 0.061	4.386AspGlu: 4.386 ± 0.073	2.774AspPhe: 2.774 ± 0.063	3.204AspGly: 3.204 ± 0.067	0.567AspHis: 0.567 ± 0.028	6.191AspIle: 6.191 ± 0.089	5.802AspLys: 5.802 ± 0.093	4.78AspLeu: 4.78 ± 0.082	1.63AspMet: 1.63 ± 0.041	3.525AspAsn: 3.525 ± 0.071	1.399AspPro: 1.399 ± 0.044	0.874AspGln: 0.874 ± 0.034	1.754AspArg: 1.754 ± 0.044	3.341AspSer: 3.341 ± 0.072	2.745AspThr: 2.745 ± 0.059	3.414AspVal: 3.414 ± 0.069	0.388AspTrp: 0.388 ± 0.021	2.634AspTyr: 2.634 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	3.935GluAla: 3.935 ± 0.071	0.6GluCys: 0.6 ± 0.031	3.861GluAsp: 3.861 ± 0.068	5.173GluGlu: 5.173 ± 0.09	2.76GluPhe: 2.76 ± 0.055	3.455GluGly: 3.455 ± 0.067	0.851GluHis: 0.851 ± 0.03	6.713GluIle: 6.713 ± 0.106	7.285GluLys: 7.285 ± 0.108	5.871GluLeu: 5.871 ± 0.083	1.827GluMet: 1.827 ± 0.048	5.149GluAsn: 5.149 ± 0.079	1.387GluPro: 1.387 ± 0.042	1.832GluGln: 1.832 ± 0.048	2.41GluArg: 2.41 ± 0.064	3.383GluSer: 3.383 ± 0.071	2.871GluThr: 2.871 ± 0.063	3.987GluVal: 3.987 ± 0.077	0.374GluTrp: 0.374 ± 0.02	2.733GluTyr: 2.733 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	2.467PheAla: 2.467 ± 0.061	0.501PheCys: 0.501 ± 0.022	2.327PheAsp: 2.327 ± 0.052	2.346PheGlu: 2.346 ± 0.052	1.929PhePhe: 1.929 ± 0.057	2.717PheGly: 2.717 ± 0.061	0.572PheHis: 0.572 ± 0.025	4.626PheIle: 4.626 ± 0.093	3.819PheLys: 3.819 ± 0.067	3.738PheLeu: 3.738 ± 0.078	1.196PheMet: 1.196 ± 0.035	3.194PheAsn: 3.194 ± 0.064	1.21PhePro: 1.21 ± 0.035	1.158PheGln: 1.158 ± 0.041	1.197PheArg: 1.197 ± 0.037	3.091PheSer: 3.091 ± 0.059	2.49PheThr: 2.49 ± 0.055	2.493PheVal: 2.493 ± 0.063	0.308PheTrp: 0.308 ± 0.019	1.801PheTyr: 1.801 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	4.167GlyAla: 4.167 ± 0.104	0.884GlyCys: 0.884 ± 0.037	3.183GlyAsp: 3.183 ± 0.063	3.894GlyGlu: 3.894 ± 0.073	3.004GlyPhe: 3.004 ± 0.061	4.094GlyGly: 4.094 ± 0.087	0.996GlyHis: 0.996 ± 0.033	6.835GlyIle: 6.835 ± 0.112	5.75GlyLys: 5.75 ± 0.096	5.167GlyLeu: 5.167 ± 0.08	1.796GlyMet: 1.796 ± 0.054	3.231GlyAsn: 3.231 ± 0.065	1.32GlyPro: 1.32 ± 0.054	1.533GlyGln: 1.533 ± 0.047	2.401GlyArg: 2.401 ± 0.066	3.931GlySer: 3.931 ± 0.081	3.63GlyThr: 3.63 ± 0.07	4.442GlyVal: 4.442 ± 0.079	0.526GlyTrp: 0.526 ± 0.03	2.914GlyTyr: 2.914 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	0.665HisAla: 0.665 ± 0.029	0.217HisCys: 0.217 ± 0.018	0.764HisAsp: 0.764 ± 0.026	0.895HisGlu: 0.895 ± 0.035	0.616HisPhe: 0.616 ± 0.029	1.096HisGly: 1.096 ± 0.039	0.29HisHis: 0.29 ± 0.021	1.368HisIle: 1.368 ± 0.041	1.181HisLys: 1.181 ± 0.039	1.108HisLeu: 1.108 ± 0.036	0.356HisMet: 0.356 ± 0.023	0.89HisAsn: 0.89 ± 0.031	0.62HisPro: 0.62 ± 0.026	0.308HisGln: 0.308 ± 0.02	0.555HisArg: 0.555 ± 0.028	0.833HisSer: 0.833 ± 0.029	0.724HisThr: 0.724 ± 0.03	0.801HisVal: 0.801 ± 0.028	0.124HisTrp: 0.124 ± 0.013	0.519HisTyr: 0.519 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.847IleAla: 5.847 ± 0.103	1.328IleCys: 1.328 ± 0.038	5.655IleAsp: 5.655 ± 0.083	6.441IleGlu: 6.441 ± 0.102	4.112IlePhe: 4.112 ± 0.088	6.069IleGly: 6.069 ± 0.098	1.33IleHis: 1.33 ± 0.043	9.749IleIle: 9.749 ± 0.139	9.208IleLys: 9.208 ± 0.111	8.659IleLeu: 8.659 ± 0.132	2.467IleMet: 2.467 ± 0.053	6.945IleAsn: 6.945 ± 0.097	3.487IlePro: 3.487 ± 0.072	2.308IleGln: 2.308 ± 0.055	3.065IleArg: 3.065 ± 0.061	7.059IleSer: 7.059 ± 0.095	5.143IleThr: 5.143 ± 0.078	5.913IleVal: 5.913 ± 0.088	0.486IleTrp: 0.486 ± 0.025	3.604IleTyr: 3.604 ± 0.076	0.0IleXaa: 0.0 ± 0.0
Lys	5.322LysAla: 5.322 ± 0.086	1.007LysCys: 1.007 ± 0.032	5.977LysAsp: 5.977 ± 0.098	7.434LysGlu: 7.434 ± 0.101	3.279LysPhe: 3.279 ± 0.062	5.192LysGly: 5.192 ± 0.078	1.144LysHis: 1.144 ± 0.034	8.548LysIle: 8.548 ± 0.11	8.262LysLys: 8.262 ± 0.115	7.906LysLeu: 7.906 ± 0.105	2.596LysMet: 2.596 ± 0.055	6.543LysAsn: 6.543 ± 0.089	2.351LysPro: 2.351 ± 0.054	2.568LysGln: 2.568 ± 0.057	3.086LysArg: 3.086 ± 0.062	5.654LysSer: 5.654 ± 0.093	4.165LysThr: 4.165 ± 0.066	5.89LysVal: 5.89 ± 0.087	0.686LysTrp: 0.686 ± 0.029	4.305LysTyr: 4.305 ± 0.078	0.0LysXaa: 0.0 ± 0.0
Leu	4.945LeuAla: 4.945 ± 0.079	1.189LeuCys: 1.189 ± 0.041	4.872LeuAsp: 4.872 ± 0.09	5.074LeuGlu: 5.074 ± 0.08	3.745LeuPhe: 3.745 ± 0.082	5.685LeuGly: 5.685 ± 0.093	1.131LeuHis: 1.131 ± 0.038	8.322LeuIle: 8.322 ± 0.128	8.95LeuLys: 8.95 ± 0.12	7.747LeuLeu: 7.747 ± 0.104	2.353LeuMet: 2.353 ± 0.051	6.364LeuAsn: 6.364 ± 0.105	2.796LeuPro: 2.796 ± 0.068	2.418LeuGln: 2.418 ± 0.05	3.062LeuArg: 3.062 ± 0.055	6.432LeuSer: 6.432 ± 0.09	4.497LeuThr: 4.497 ± 0.076	4.878LeuVal: 4.878 ± 0.08	0.563LeuTrp: 0.563 ± 0.028	3.319LeuTyr: 3.319 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	1.903MetAla: 1.903 ± 0.049	0.317MetCys: 0.317 ± 0.018	1.868MetAsp: 1.868 ± 0.042	1.957MetGlu: 1.957 ± 0.05	0.985MetPhe: 0.985 ± 0.032	1.875MetGly: 1.875 ± 0.049	0.411MetHis: 0.411 ± 0.023	2.181MetIle: 2.181 ± 0.051	2.539MetLys: 2.539 ± 0.055	2.466MetLeu: 2.466 ± 0.05	0.706MetMet: 0.706 ± 0.032	1.821MetAsn: 1.821 ± 0.05	0.978MetPro: 0.978 ± 0.033	0.746MetGln: 0.746 ± 0.033	0.881MetArg: 0.881 ± 0.03	1.679MetSer: 1.679 ± 0.045	1.16MetThr: 1.16 ± 0.036	1.703MetVal: 1.703 ± 0.046	0.163MetTrp: 0.163 ± 0.014	0.945MetTyr: 0.945 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.239AsnAla: 3.239 ± 0.061	0.85AsnCys: 0.85 ± 0.04	3.145AsnAsp: 3.145 ± 0.065	4.225AsnGlu: 4.225 ± 0.081	2.625AsnPhe: 2.625 ± 0.062	4.005AsnGly: 4.005 ± 0.075	0.838AsnHis: 0.838 ± 0.038	7.174AsnIle: 7.174 ± 0.096	6.42AsnLys: 6.42 ± 0.092	5.572AsnLeu: 5.572 ± 0.089	1.842AsnMet: 1.842 ± 0.05	4.983AsnAsn: 4.983 ± 0.1	2.034AsnPro: 2.034 ± 0.049	1.457AsnGln: 1.457 ± 0.039	1.933AsnArg: 1.933 ± 0.048	4.316AsnSer: 4.316 ± 0.085	3.101AsnThr: 3.101 ± 0.063	3.915AsnVal: 3.915 ± 0.063	0.451AsnTrp: 0.451 ± 0.023	2.838AsnTyr: 2.838 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.678ProAla: 1.678 ± 0.051	0.367ProCys: 0.367 ± 0.023	1.74ProAsp: 1.74 ± 0.04	2.188ProGlu: 2.188 ± 0.052	1.401ProPhe: 1.401 ± 0.042	1.944ProGly: 1.944 ± 0.062	0.49ProHis: 0.49 ± 0.024	2.587ProIle: 2.587 ± 0.065	2.256ProLys: 2.256 ± 0.05	2.601ProLeu: 2.601 ± 0.059	0.784ProMet: 0.784 ± 0.032	1.456ProAsn: 1.456 ± 0.044	0.68ProPro: 0.68 ± 0.036	0.791ProGln: 0.791 ± 0.028	0.863ProArg: 0.863 ± 0.037	1.802ProSer: 1.802 ± 0.047	1.372ProThr: 1.372 ± 0.036	2.344ProVal: 2.344 ± 0.057	0.256ProTrp: 0.256 ± 0.019	1.381ProTyr: 1.381 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	1.522GlnAla: 1.522 ± 0.048	0.352GlnCys: 0.352 ± 0.021	1.344GlnAsp: 1.344 ± 0.044	1.379GlnGlu: 1.379 ± 0.042	0.999GlnPhe: 0.999 ± 0.034	1.712GlnGly: 1.712 ± 0.048	0.413GlnHis: 0.413 ± 0.021	2.375GlnIle: 2.375 ± 0.056	2.058GlnLys: 2.058 ± 0.064	2.433GlnLeu: 2.433 ± 0.045	0.76GlnMet: 0.76 ± 0.028	1.505GlnAsn: 1.505 ± 0.043	0.676GlnPro: 0.676 ± 0.032	0.978GlnGln: 0.978 ± 0.041	1.01GlnArg: 1.01 ± 0.039	1.499GlnSer: 1.499 ± 0.033	1.043GlnThr: 1.043 ± 0.037	1.621GlnVal: 1.621 ± 0.038	0.306GlnTrp: 0.306 ± 0.021	1.138GlnTyr: 1.138 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	1.866ArgAla: 1.866 ± 0.045	0.413ArgCys: 0.413 ± 0.024	1.788ArgAsp: 1.788 ± 0.046	2.602ArgGlu: 2.602 ± 0.064	1.473ArgPhe: 1.473 ± 0.041	2.069ArgGly: 2.069 ± 0.056	0.546ArgHis: 0.546 ± 0.024	3.102ArgIle: 3.102 ± 0.059	3.186ArgLys: 3.186 ± 0.062	2.951ArgLeu: 2.951 ± 0.061	0.948ArgMet: 0.948 ± 0.034	2.116ArgAsn: 2.116 ± 0.051	0.987ArgPro: 0.987 ± 0.039	1.033ArgGln: 1.033 ± 0.033	1.461ArgArg: 1.461 ± 0.053	1.732ArgSer: 1.732 ± 0.045	1.626ArgThr: 1.626 ± 0.045	2.101ArgVal: 2.101 ± 0.057	0.257ArgTrp: 0.257 ± 0.018	1.416ArgTyr: 1.416 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	3.659SerAla: 3.659 ± 0.068	0.757SerCys: 0.757 ± 0.032	3.336SerAsp: 3.336 ± 0.064	3.956SerGlu: 3.956 ± 0.069	3.188SerPhe: 3.188 ± 0.057	4.501SerGly: 4.501 ± 0.077	0.946SerHis: 0.946 ± 0.032	6.653SerIle: 6.653 ± 0.101	5.748SerLys: 5.748 ± 0.079	5.765SerLeu: 5.765 ± 0.092	1.745SerMet: 1.745 ± 0.045	3.872SerAsn: 3.872 ± 0.078	1.732SerPro: 1.732 ± 0.049	1.664SerGln: 1.664 ± 0.046	2.385SerArg: 2.385 ± 0.064	4.855SerSer: 4.855 ± 0.098	3.136SerThr: 3.136 ± 0.069	4.021SerVal: 4.021 ± 0.068	0.443SerTrp: 0.443 ± 0.026	2.653SerTyr: 2.653 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	3.459ThrAla: 3.459 ± 0.066	0.557ThrCys: 0.557 ± 0.026	2.572ThrAsp: 2.572 ± 0.055	2.946ThrGlu: 2.946 ± 0.061	2.069ThrPhe: 2.069 ± 0.054	3.88ThrGly: 3.88 ± 0.071	0.727ThrHis: 0.727 ± 0.03	4.812ThrIle: 4.812 ± 0.075	3.665ThrLys: 3.665 ± 0.069	4.564ThrLeu: 4.564 ± 0.078	1.197ThrMet: 1.197 ± 0.037	2.653ThrAsn: 2.653 ± 0.065	1.794ThrPro: 1.794 ± 0.054	1.123ThrGln: 1.123 ± 0.037	1.538ThrArg: 1.538 ± 0.041	3.02ThrSer: 3.02 ± 0.061	2.48ThrThr: 2.48 ± 0.061	3.49ThrVal: 3.49 ± 0.063	0.346ThrTrp: 0.346 ± 0.021	1.865ThrTyr: 1.865 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	3.514ValAla: 3.514 ± 0.072	0.822ValCys: 0.822 ± 0.032	3.726ValAsp: 3.726 ± 0.06	3.945ValGlu: 3.945 ± 0.072	2.713ValPhe: 2.713 ± 0.062	3.822ValGly: 3.822 ± 0.077	0.944ValHis: 0.944 ± 0.04	6.446ValIle: 6.446 ± 0.092	5.768ValLys: 5.768 ± 0.086	5.823ValLeu: 5.823 ± 0.083	1.747ValMet: 1.747 ± 0.042	3.993ValAsn: 3.993 ± 0.073	2.173ValPro: 2.173 ± 0.055	1.581ValGln: 1.581 ± 0.051	2.012ValArg: 2.012 ± 0.048	4.491ValSer: 4.491 ± 0.07	3.272ValThr: 3.272 ± 0.073	4.215ValVal: 4.215 ± 0.075	0.381ValTrp: 0.381 ± 0.024	2.451ValTyr: 2.451 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.381TrpAla: 0.381 ± 0.022	0.124TrpCys: 0.124 ± 0.012	0.394TrpAsp: 0.394 ± 0.021	0.371TrpGlu: 0.371 ± 0.025	0.31TrpPhe: 0.31 ± 0.017	0.446TrpGly: 0.446 ± 0.024	0.128TrpHis: 0.128 ± 0.011	0.665TrpIle: 0.665 ± 0.032	0.566TrpLys: 0.566 ± 0.026	0.555TrpLeu: 0.555 ± 0.027	0.231TrpMet: 0.231 ± 0.017	0.459TrpAsn: 0.459 ± 0.024	0.186TrpPro: 0.186 ± 0.014	0.247TrpGln: 0.247 ± 0.019	0.266TrpArg: 0.266 ± 0.017	0.41TrpSer: 0.41 ± 0.026	0.31TrpThr: 0.31 ± 0.021	0.433TrpVal: 0.433 ± 0.023	0.078TrpTrp: 0.078 ± 0.011	0.338TrpTyr: 0.338 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.146TyrAla: 2.146 ± 0.052	0.601TyrCys: 0.601 ± 0.027	2.425TyrAsp: 2.425 ± 0.06	2.638TyrGlu: 2.638 ± 0.053	2.052TyrPhe: 2.052 ± 0.055	2.588TyrGly: 2.588 ± 0.061	0.526TyrHis: 0.526 ± 0.025	4.062TyrIle: 4.062 ± 0.076	3.741TyrLys: 3.741 ± 0.079	3.68TyrLeu: 3.68 ± 0.08	1.036TyrMet: 1.036 ± 0.033	3.005TyrAsn: 3.005 ± 0.072	1.25TyrPro: 1.25 ± 0.039	0.749TyrGln: 0.749 ± 0.034	1.436TyrArg: 1.436 ± 0.045	2.948TyrSer: 2.948 ± 0.058	2.099TyrThr: 2.099 ± 0.054	2.252TyrVal: 2.252 ± 0.048	0.32TyrTrp: 0.32 ± 0.021	1.96TyrTyr: 1.96 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2864 proteins (876206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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