Grapevine associated tymo-like virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; unclassified Tymovirales

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A385L3F5|A0A385L3F5_9VIRU RNA-directed RNA polymerase OS=Grapevine associated tymo-like virus OX=2338396 PE=4 SV=1
MM1 pKa = 8.2SEE3 pKa = 3.89ITPVPSNSNMSRR15 pKa = 11.84TSSARR20 pKa = 11.84VFGGKK25 pKa = 9.29FADD28 pKa = 3.75LTSNVLVQHH37 pKa = 6.34NLHH40 pKa = 5.64NHH42 pKa = 4.86AVVMNVMSAYY52 pKa = 9.87SNVRR56 pKa = 11.84ILEE59 pKa = 3.99LSVFPVLLPAARR71 pKa = 11.84DD72 pKa = 3.84FNVNLYY78 pKa = 10.05VGWGHH83 pKa = 6.76CEE85 pKa = 3.34HH86 pKa = 6.9TAPEE90 pKa = 4.17NVAQLAQLPCFKK102 pKa = 10.39VYY104 pKa = 10.64SSSDD108 pKa = 3.11QGFGGEE114 pKa = 4.04PLVCEE119 pKa = 4.68FDD121 pKa = 3.58MSIVDD126 pKa = 3.61QLKK129 pKa = 9.93PIPLEE134 pKa = 3.93GGRR137 pKa = 11.84PKK139 pKa = 10.76LFAVAITSSKK149 pKa = 10.31EE150 pKa = 3.95RR151 pKa = 11.84ISVAEE156 pKa = 3.61LFFRR160 pKa = 11.84VKK162 pKa = 10.65VQVDD166 pKa = 4.23GYY168 pKa = 10.99DD169 pKa = 2.85IVFAA173 pKa = 5.18

Molecular weight:
18.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A385L3F5|A0A385L3F5_9VIRU RNA-directed RNA polymerase OS=Grapevine associated tymo-like virus OX=2338396 PE=4 SV=1
MM1 pKa = 7.43FNFEE5 pKa = 4.44VKK7 pKa = 10.44SIAIVAGNNVHH18 pKa = 6.91AFGVFLKK25 pKa = 10.49PRR27 pKa = 11.84LNSLIRR33 pKa = 11.84GGGRR37 pKa = 11.84GSDD40 pKa = 4.03GINDD44 pKa = 4.22GVNSMGTTMHH54 pKa = 7.19RR55 pKa = 11.84DD56 pKa = 3.07AVTQDD61 pKa = 2.61IANRR65 pKa = 11.84LINDD69 pKa = 3.0IAEE72 pKa = 4.36AKK74 pKa = 9.34EE75 pKa = 3.55ICPYY79 pKa = 10.93SMSPAAVDD87 pKa = 3.35MLLTIGIPASRR98 pKa = 11.84SFTASHH104 pKa = 6.76RR105 pKa = 11.84HH106 pKa = 5.47PMHH109 pKa = 6.94KK110 pKa = 10.35SLEE113 pKa = 3.91NWILRR118 pKa = 11.84KK119 pKa = 9.69VVPNLVTFDD128 pKa = 3.98CTVAWMKK135 pKa = 9.99PVKK138 pKa = 10.06FDD140 pKa = 3.42RR141 pKa = 11.84LSNNEE146 pKa = 4.02PKK148 pKa = 10.48FKK150 pKa = 10.84QLVNPVITPRR160 pKa = 11.84DD161 pKa = 3.45FARR164 pKa = 11.84YY165 pKa = 9.26RR166 pKa = 11.84DD167 pKa = 3.94TKK169 pKa = 10.51RR170 pKa = 11.84VASLTQSLLIHH181 pKa = 7.1DD182 pKa = 4.78AMHH185 pKa = 5.95YY186 pKa = 8.13MSKK189 pKa = 10.78EE190 pKa = 3.68EE191 pKa = 4.13VYY193 pKa = 11.15GLFVEE198 pKa = 5.28NPDD201 pKa = 3.71LQEE204 pKa = 4.14VIATVVYY211 pKa = 9.35PCEE214 pKa = 3.83VLKK217 pKa = 10.66RR218 pKa = 11.84QQSLFPLLYY227 pKa = 10.52DD228 pKa = 3.58LTYY231 pKa = 9.06TKK233 pKa = 10.72EE234 pKa = 3.97GFVYY238 pKa = 10.82SMEE241 pKa = 5.1GDD243 pKa = 4.19DD244 pKa = 3.95NDD246 pKa = 5.9HH247 pKa = 6.5YY248 pKa = 10.75VQPYY252 pKa = 8.37NDD254 pKa = 3.24WVITTKK260 pKa = 11.08YY261 pKa = 10.42LVGKK265 pKa = 6.99MFRR268 pKa = 11.84ITIEE272 pKa = 4.12RR273 pKa = 11.84IEE275 pKa = 4.49GFFGHH280 pKa = 6.43HH281 pKa = 5.29VFVLRR286 pKa = 11.84RR287 pKa = 11.84GNYY290 pKa = 7.88RR291 pKa = 11.84APDD294 pKa = 3.76HH295 pKa = 6.44YY296 pKa = 10.74LSPMPNCVLLPDD308 pKa = 4.35TNLLMKK314 pKa = 10.32GEE316 pKa = 4.3RR317 pKa = 11.84EE318 pKa = 3.7RR319 pKa = 11.84LVPRR323 pKa = 11.84EE324 pKa = 4.07IYY326 pKa = 10.62DD327 pKa = 3.59KK328 pKa = 11.01AVHH331 pKa = 6.35HH332 pKa = 6.32ARR334 pKa = 11.84NLKK337 pKa = 10.09ALRR340 pKa = 11.84AQDD343 pKa = 4.89AIAKK347 pKa = 8.4VRR349 pKa = 11.84SYY351 pKa = 10.69QHH353 pKa = 6.82DD354 pKa = 3.72SAYY357 pKa = 10.7GWVDD361 pKa = 3.69PGAWEE366 pKa = 3.88YY367 pKa = 9.57LTRR370 pKa = 11.84YY371 pKa = 8.84VISAVAVLGDD381 pKa = 3.67SPDD384 pKa = 3.29IVSEE388 pKa = 3.94IDD390 pKa = 3.07THH392 pKa = 7.02PFILKK397 pKa = 10.46LKK399 pKa = 9.93ALWKK403 pKa = 10.48NRR405 pKa = 11.84GFKK408 pKa = 9.53LTLLVVSGGLACVLTPTILCWQLIPLATWIAKK440 pKa = 9.85IIVGSEE446 pKa = 3.52LGLAGFVAYY455 pKa = 9.5KK456 pKa = 10.17IWPHH460 pKa = 5.42WLSPAEE466 pKa = 4.08RR467 pKa = 11.84EE468 pKa = 4.26RR469 pKa = 11.84KK470 pKa = 9.1RR471 pKa = 11.84LAWLYY476 pKa = 9.4PKK478 pKa = 10.38RR479 pKa = 11.84HH480 pKa = 6.07TDD482 pKa = 4.47LILLEE487 pKa = 4.06EE488 pKa = 4.43RR489 pKa = 11.84KK490 pKa = 9.51ISQHH494 pKa = 4.87QVHH497 pKa = 6.5EE498 pKa = 4.59LLCDD502 pKa = 3.63MLSEE506 pKa = 4.32GAEE509 pKa = 4.07FPTFTPIPKK518 pKa = 9.96AKK520 pKa = 9.42PDD522 pKa = 3.72SLISNGTDD530 pKa = 3.0QSDD533 pKa = 3.77PGPSVSEE540 pKa = 4.0VACAKK545 pKa = 10.9NEE547 pKa = 3.73VDD549 pKa = 4.23LDD551 pKa = 3.99SVATVGSSANFTVTLRR567 pKa = 11.84KK568 pKa = 9.92NSGVGEE574 pKa = 3.97RR575 pKa = 11.84DD576 pKa = 3.18CALMNEE582 pKa = 4.51EE583 pKa = 4.13NKK585 pKa = 10.38HH586 pKa = 5.15VEE588 pKa = 4.03EE589 pKa = 5.8SEE591 pKa = 4.44DD592 pKa = 3.41SLFAEE597 pKa = 4.8KK598 pKa = 10.77SKK600 pKa = 11.07LNCSSTFTAEE610 pKa = 4.23TVEE613 pKa = 4.14HH614 pKa = 6.71FSLKK618 pKa = 9.91SDD620 pKa = 3.24EE621 pKa = 4.75TITVRR626 pKa = 11.84LPKK629 pKa = 9.99IVLTRR634 pKa = 11.84VKK636 pKa = 10.41DD637 pKa = 3.79LKK639 pKa = 11.26SEE641 pKa = 4.43DD642 pKa = 3.73KK643 pKa = 11.32VVMDD647 pKa = 4.82KK648 pKa = 11.52NFLTVAGAEE657 pKa = 4.17LKK659 pKa = 10.61DD660 pKa = 3.82EE661 pKa = 4.2KK662 pKa = 11.26VADD665 pKa = 3.39ISLGLNEE672 pKa = 4.68EE673 pKa = 4.56VITKK677 pKa = 9.86KK678 pKa = 10.4KK679 pKa = 9.86KK680 pKa = 8.92KK681 pKa = 10.3NRR683 pKa = 11.84VKK685 pKa = 10.64RR686 pKa = 11.84NKK688 pKa = 10.12KK689 pKa = 9.91KK690 pKa = 10.68DD691 pKa = 3.46GSDD694 pKa = 3.51VNSHH698 pKa = 6.64LEE700 pKa = 3.76QVTIIPAEE708 pKa = 4.68GIDD711 pKa = 4.18GEE713 pKa = 4.69GWLHH717 pKa = 6.35KK718 pKa = 10.45RR719 pKa = 11.84FGVADD724 pKa = 4.15DD725 pKa = 4.07VALGQYY731 pKa = 9.26SNCADD736 pKa = 4.54DD737 pKa = 5.95EE738 pKa = 4.46ILFDD742 pKa = 4.38GDD744 pKa = 3.68FEE746 pKa = 4.58EE747 pKa = 6.29VPLCLLDD754 pKa = 4.4ALEE757 pKa = 4.53NSTGVSRR764 pKa = 11.84NIMIEE769 pKa = 3.82ALRR772 pKa = 11.84ILPKK776 pKa = 10.29KK777 pKa = 10.24SVVNEE782 pKa = 3.7EE783 pKa = 4.21TKK785 pKa = 10.67RR786 pKa = 11.84EE787 pKa = 4.04GLSDD791 pKa = 2.73WHH793 pKa = 6.48AAALAYY799 pKa = 7.85YY800 pKa = 8.31FCWEE804 pKa = 4.08LNIYY808 pKa = 8.01NAWVKK813 pKa = 10.61RR814 pKa = 11.84VMGVRR819 pKa = 11.84NGAKK823 pKa = 9.3VDD825 pKa = 2.98IWHH828 pKa = 6.13VMVGSMGHH836 pKa = 5.03FTHH839 pKa = 6.12VRR841 pKa = 11.84PRR843 pKa = 11.84TATLAKK849 pKa = 9.79GAEE852 pKa = 4.32PGSLHH857 pKa = 6.31GASLGTKK864 pKa = 9.53RR865 pKa = 11.84FQEE868 pKa = 4.34ALLAWRR874 pKa = 11.84GGDD877 pKa = 3.42DD878 pKa = 3.72SAIPFLGFRR887 pKa = 11.84GHH889 pKa = 5.86VVDD892 pKa = 4.08VEE894 pKa = 4.2RR895 pKa = 11.84ARR897 pKa = 11.84FLARR901 pKa = 11.84DD902 pKa = 3.68MANGRR907 pKa = 11.84TGILFGPAEE916 pKa = 4.71KK917 pKa = 9.8STMDD921 pKa = 3.51SDD923 pKa = 3.91TQVRR927 pKa = 11.84WKK929 pKa = 10.64FLVDD933 pKa = 3.63GQEE936 pKa = 3.68QRR938 pKa = 11.84LYY940 pKa = 9.06KK941 pKa = 10.24HH942 pKa = 6.5RR943 pKa = 11.84ARR945 pKa = 11.84VHH947 pKa = 4.76VAAIYY952 pKa = 9.12GYY954 pKa = 8.76PGCGKK959 pKa = 7.87TWPLAQVLNKK969 pKa = 10.05VKK971 pKa = 10.66GLDD974 pKa = 3.44YY975 pKa = 10.5RR976 pKa = 11.84VAVPTVKK983 pKa = 10.46LRR985 pKa = 11.84DD986 pKa = 3.15EE987 pKa = 4.45WLNMLQLVQHH997 pKa = 6.27EE998 pKa = 4.43KK999 pKa = 10.13WRR1001 pKa = 11.84VNTYY1005 pKa = 9.99EE1006 pKa = 3.56STMRR1010 pKa = 11.84KK1011 pKa = 7.13NTEE1014 pKa = 3.45ILIVDD1019 pKa = 5.6EE1020 pKa = 4.45ISMMPSGYY1028 pKa = 10.39VDD1030 pKa = 3.96FLIAISPRR1038 pKa = 11.84LRR1040 pKa = 11.84CVLILGDD1047 pKa = 3.46VTQTDD1052 pKa = 3.61RR1053 pKa = 11.84HH1054 pKa = 5.94EE1055 pKa = 4.33SHH1057 pKa = 7.67PDD1059 pKa = 3.18ASCSRR1064 pKa = 11.84LLAEE1068 pKa = 3.73SLYY1071 pKa = 8.37WRR1073 pKa = 11.84KK1074 pKa = 9.9YY1075 pKa = 8.67SPFYY1079 pKa = 10.86LGFTRR1084 pKa = 11.84RR1085 pKa = 11.84LSRR1088 pKa = 11.84HH1089 pKa = 4.11TAKK1092 pKa = 10.86LFGVNTFSTVEE1103 pKa = 3.83GADD1106 pKa = 3.62FRR1108 pKa = 11.84RR1109 pKa = 11.84EE1110 pKa = 3.78TRR1112 pKa = 11.84GPGVILTALSGDD1124 pKa = 3.25VRR1126 pKa = 11.84MLAEE1130 pKa = 5.32LGQSACTFPSSQGSTFDD1147 pKa = 4.99DD1148 pKa = 4.03YY1149 pKa = 12.08VQILMDD1155 pKa = 4.16RR1156 pKa = 11.84SALEE1160 pKa = 4.07KK1161 pKa = 10.56CSLGAVHH1168 pKa = 6.5TAVTRR1173 pKa = 11.84SRR1175 pKa = 11.84KK1176 pKa = 10.0GNILVGRR1183 pKa = 11.84LTGLWWKK1190 pKa = 10.35AAISNPFWRR1199 pKa = 11.84AILLNEE1205 pKa = 4.21PCDD1208 pKa = 3.59WRR1210 pKa = 11.84IVFSDD1215 pKa = 3.37VLQDD1219 pKa = 4.15FHH1221 pKa = 8.73ILNGPYY1227 pKa = 9.88KK1228 pKa = 9.89PNKK1231 pKa = 9.87LSGGLLVEE1239 pKa = 4.87HH1240 pKa = 6.9GFPLDD1245 pKa = 3.32MLPPMRR1251 pKa = 11.84RR1252 pKa = 11.84VGHH1255 pKa = 5.51TCLVEE1260 pKa = 3.8EE1261 pKa = 4.97LPIEE1265 pKa = 4.19VEE1267 pKa = 4.05AFDD1270 pKa = 4.51EE1271 pKa = 4.49EE1272 pKa = 5.33VVDD1275 pKa = 4.82EE1276 pKa = 4.45PARR1279 pKa = 11.84VTWGQMDD1286 pKa = 4.1AKK1288 pKa = 10.89VLFDD1292 pKa = 3.49SVYY1295 pKa = 10.89KK1296 pKa = 10.54LRR1298 pKa = 11.84PVDD1301 pKa = 3.21AVSRR1305 pKa = 11.84LRR1307 pKa = 11.84RR1308 pKa = 11.84YY1309 pKa = 9.84KK1310 pKa = 10.85GEE1312 pKa = 3.98PSKK1315 pKa = 10.61QFCDD1319 pKa = 3.98GEE1321 pKa = 4.32PEE1323 pKa = 3.9GRR1325 pKa = 11.84NEE1327 pKa = 4.04DD1328 pKa = 3.64GIYY1331 pKa = 10.37PEE1333 pKa = 4.46MVAARR1338 pKa = 11.84HH1339 pKa = 5.53KK1340 pKa = 10.82RR1341 pKa = 11.84ADD1343 pKa = 3.71DD1344 pKa = 3.49TLLPLSVEE1352 pKa = 3.38KK1353 pKa = 10.61RR1354 pKa = 11.84LRR1356 pKa = 11.84FRR1358 pKa = 11.84PLAANKK1364 pKa = 10.31RR1365 pKa = 11.84EE1366 pKa = 4.03VLSKK1370 pKa = 11.05SLVGQEE1376 pKa = 4.72LFQSYY1381 pKa = 9.66CRR1383 pKa = 11.84LMEE1386 pKa = 5.13LDD1388 pKa = 3.68PLRR1391 pKa = 11.84PFSFNEE1397 pKa = 3.69ILYY1400 pKa = 10.05HH1401 pKa = 7.08DD1402 pKa = 5.42CINFNEE1408 pKa = 4.3WHH1410 pKa = 6.52SLTNKK1415 pKa = 6.74TQAMILANEE1424 pKa = 4.4NKK1426 pKa = 9.94SDD1428 pKa = 4.57PDD1430 pKa = 3.81WVWTFIRR1437 pKa = 11.84VFMKK1441 pKa = 9.2QQRR1444 pKa = 11.84KK1445 pKa = 9.71INDD1448 pKa = 3.29STLNGDD1454 pKa = 3.46WKK1456 pKa = 10.95AGQTISNMNDD1466 pKa = 2.32QWLLYY1471 pKa = 10.0LGPTIRR1477 pKa = 11.84YY1478 pKa = 6.74MSKK1481 pKa = 10.7LEE1483 pKa = 4.18KK1484 pKa = 10.12KK1485 pKa = 9.06YY1486 pKa = 10.34CPKK1489 pKa = 10.42RR1490 pKa = 11.84IYY1492 pKa = 10.83LHH1494 pKa = 6.68GGRR1497 pKa = 11.84SNIDD1501 pKa = 3.18LDD1503 pKa = 3.89NFCRR1507 pKa = 11.84DD1508 pKa = 3.17NLTVGKK1514 pKa = 10.11KK1515 pKa = 9.2YY1516 pKa = 11.09ANDD1519 pKa = 3.44YY1520 pKa = 10.58TSFDD1524 pKa = 3.31QSQTGEE1530 pKa = 4.13VLASEE1535 pKa = 5.08LLYY1538 pKa = 9.55MWHH1541 pKa = 6.94AGIPEE1546 pKa = 4.16VIINCYY1552 pKa = 9.46EE1553 pKa = 4.08SNKK1556 pKa = 9.75VDD1558 pKa = 3.38MFCAFGHH1565 pKa = 5.78LQTMRR1570 pKa = 11.84FSGEE1574 pKa = 3.56PATYY1578 pKa = 10.83AFNCRR1583 pKa = 11.84CNLAVLNLQFPLDD1596 pKa = 3.81RR1597 pKa = 11.84NLNVPIFVSGDD1608 pKa = 3.35DD1609 pKa = 3.89GGLGTVLPEE1618 pKa = 3.67RR1619 pKa = 11.84STWNMISNHH1628 pKa = 5.62LTLQAKK1634 pKa = 9.26PLISDD1639 pKa = 3.6RR1640 pKa = 11.84MVFVGYY1646 pKa = 10.16ICTHH1650 pKa = 6.73LGAIRR1655 pKa = 11.84DD1656 pKa = 4.29PVPMLARR1663 pKa = 11.84FYY1665 pKa = 11.06LADD1668 pKa = 4.22DD1669 pKa = 4.19AGKK1672 pKa = 9.61LHH1674 pKa = 6.87QIVQSYY1680 pKa = 7.99ATEE1683 pKa = 4.39LSTGYY1688 pKa = 11.25LMAEE1692 pKa = 4.62GVFEE1696 pKa = 4.04MLNDD1700 pKa = 4.07FEE1702 pKa = 5.84LDD1704 pKa = 3.67CFFCLVRR1711 pKa = 11.84AFHH1714 pKa = 6.83LKK1716 pKa = 9.96HH1717 pKa = 6.12ASSRR1721 pKa = 11.84LKK1723 pKa = 10.24FRR1725 pKa = 11.84KK1726 pKa = 9.35SALLGFVNLSEE1737 pKa = 4.31KK1738 pKa = 10.95ALGRR1742 pKa = 11.84VGAGARR1748 pKa = 11.84LGPDD1752 pKa = 3.3LRR1754 pKa = 11.84RR1755 pKa = 11.84DD1756 pKa = 3.29LVSYY1760 pKa = 8.59YY1761 pKa = 9.66WRR1763 pKa = 11.84LTEE1766 pKa = 3.87NEE1768 pKa = 3.9RR1769 pKa = 11.84AGVEE1773 pKa = 4.03KK1774 pKa = 10.48QVQNLVNANLYY1785 pKa = 10.28EE1786 pKa = 4.35FGRR1789 pKa = 11.84RR1790 pKa = 3.49

Molecular weight:
202.83 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1963

173

1790

981.5

110.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.928 ± 0.248

1.783 ± 0.021

5.909 ± 0.79

6.011 ± 0.343

4.483 ± 0.795

6.368 ± 0.249

2.802 ± 0.037

4.585 ± 0.228

5.553 ± 0.883

10.494 ± 0.773

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.145

4.687 ± 0.463

4.534 ± 0.528

2.598 ± 0.369

6.419 ± 1.005

6.47 ± 1.423

4.687 ± 0.761

8.253 ± 1.892

1.834 ± 0.532

3.057 ± 0.315

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski