Vagococcus coleopterorum
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1442 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8AKU5|A0A6G8AKU5_9ENTE Thioredoxin OS=Vagococcus coleopterorum OX=2714946 GN=trxA PE=3 SV=1
MM1 pKa = 7.16 TLAKK5 pKa = 9.5 IVYY8 pKa = 10.15 ASLTGNTEE16 pKa = 3.78 EE17 pKa = 5.22 LADD20 pKa = 4.02 TVAEE24 pKa = 3.98 ALEE27 pKa = 4.25 NFDD30 pKa = 4.67 IEE32 pKa = 4.74 VEE34 pKa = 4.18 QEE36 pKa = 3.45 EE37 pKa = 4.93 CTQVDD42 pKa = 3.59 AEE44 pKa = 4.83 DD45 pKa = 4.13 FADD48 pKa = 4.3 ADD50 pKa = 3.6 ICIVATYY57 pKa = 9.24 TYY59 pKa = 11.29 GEE61 pKa = 4.38 GDD63 pKa = 4.04 LPDD66 pKa = 4.72 EE67 pKa = 4.29 IVDD70 pKa = 4.34 FFEE73 pKa = 5.6 DD74 pKa = 3.9 LQDD77 pKa = 3.16 VDD79 pKa = 5.51 LDD81 pKa = 3.86 GKK83 pKa = 10.64 IYY85 pKa = 10.56 GVVGSGDD92 pKa = 3.5 TFYY95 pKa = 11.45 EE96 pKa = 4.83 MYY98 pKa = 9.96 CKK100 pKa = 10.54 SVDD103 pKa = 3.56 DD104 pKa = 4.74 FEE106 pKa = 5.9 AAFEE110 pKa = 4.11 KK111 pKa = 10.33 TGAKK115 pKa = 9.93 KK116 pKa = 10.39 GATCVKK122 pKa = 9.76 VDD124 pKa = 4.0 LAVEE128 pKa = 4.16 EE129 pKa = 4.56 EE130 pKa = 4.74 DD131 pKa = 3.37 IQNVEE136 pKa = 4.1 RR137 pKa = 11.84 FAKK140 pKa = 10.19 EE141 pKa = 3.16 IAAHH145 pKa = 6.42 FEE147 pKa = 4.02
Molecular weight: 16.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.834
Wikipedia 3.554
Rodwell 3.503
Grimsley 3.376
Solomon 3.63
Lehninger 3.592
Nozaki 3.757
DTASelect 3.948
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.783
Patrickios 1.812
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A6G8ALZ1|A0A6G8ALZ1_9ENTE Nicotinate phosphoribosyltransferase OS=Vagococcus coleopterorum OX=2714946 GN=G7081_02845 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 8.35 HH12 pKa = 6.42 AKK14 pKa = 8.54 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.8 GRR39 pKa = 11.84 KK40 pKa = 8.35 VLCSS44 pKa = 3.34
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1442
0
1442
450470
29
1675
312.4
34.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.148 ± 0.071
0.626 ± 0.015
5.616 ± 0.055
7.675 ± 0.076
4.41 ± 0.051
6.733 ± 0.057
1.739 ± 0.027
7.49 ± 0.061
7.083 ± 0.053
9.794 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.876 ± 0.033
4.609 ± 0.044
3.427 ± 0.034
3.805 ± 0.052
3.585 ± 0.047
6.013 ± 0.046
5.867 ± 0.042
7.268 ± 0.048
0.826 ± 0.021
3.41 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here