Escherichia phage K1G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; Escherichia virus K1G

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XJ93|D2XJ93_9CAUD Uncharacterized protein OS=Escherichia phage K1G OX=698486 PE=4 SV=1
MM1 pKa = 6.9SQEE4 pKa = 4.31SVEE7 pKa = 3.72AAYY10 pKa = 10.18RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.99LASNPDD19 pKa = 3.03GEE21 pKa = 4.43MDD23 pKa = 4.62YY24 pKa = 9.86ITLQISHH31 pKa = 7.73PLLSKK36 pKa = 9.5TYY38 pKa = 8.28YY39 pKa = 10.26LVRR42 pKa = 11.84GLQEE46 pKa = 3.63LTATLEE52 pKa = 4.05TGEE55 pKa = 4.7TITFEE60 pKa = 4.24PTPMEE65 pKa = 4.29ASGAANNSDD74 pKa = 3.47MDD76 pKa = 3.63QTTTFTLPDD85 pKa = 3.96ILNQLDD91 pKa = 4.0DD92 pKa = 4.59EE93 pKa = 4.37MDD95 pKa = 4.93KK96 pKa = 11.08IPMSNTEE103 pKa = 3.69LPKK106 pKa = 10.39FVFRR110 pKa = 11.84RR111 pKa = 11.84YY112 pKa = 9.99VSTDD116 pKa = 2.79LSYY119 pKa = 10.54PADD122 pKa = 3.77GPVVYY127 pKa = 9.79EE128 pKa = 3.91LQAINQEE135 pKa = 4.09NGEE138 pKa = 4.1FSADD142 pKa = 3.08VGTPMLNQRR151 pKa = 11.84STGILMTPKK160 pKa = 10.28EE161 pKa = 4.17IPLLRR166 pKa = 11.84GLLTTT171 pKa = 4.94

Molecular weight: 19.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XJA0|D2XJA0_9CAUD Uncharacterized protein OS=Escherichia phage K1G OX=698486 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 9.71EE3 pKa = 4.14ALPKK7 pKa = 10.57LSQKK11 pKa = 9.0TLTGSPSVIFSRR23 pKa = 11.84VSAAGLSRR31 pKa = 11.84SDD33 pKa = 3.17LRR35 pKa = 11.84EE36 pKa = 3.59FRR38 pKa = 11.84KK39 pKa = 9.09MRR41 pKa = 11.84LSGRR45 pKa = 11.84EE46 pKa = 3.5ARR48 pKa = 11.84LANRR52 pKa = 11.84LARR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 4.31SNSDD61 pKa = 2.53SSTRR65 pKa = 11.84DD66 pKa = 2.99TSRR69 pKa = 11.84RR70 pKa = 11.84SSSTSSASAALQSSLGSRR88 pKa = 11.84LRR90 pKa = 11.84QQLQNRR96 pKa = 11.84GCAIYY101 pKa = 10.86KK102 pKa = 7.48LTWKK106 pKa = 10.51EE107 pKa = 3.58KK108 pKa = 6.33TTPSGLLYY116 pKa = 10.41SQLVASAHH124 pKa = 4.65RR125 pKa = 11.84TKK127 pKa = 11.12EE128 pKa = 3.91NDD130 pKa = 3.02SSLARR135 pKa = 11.84TNWPTPTANDD145 pKa = 3.34FRR147 pKa = 11.84GSGEE151 pKa = 4.22TVIRR155 pKa = 11.84TDD157 pKa = 3.09GRR159 pKa = 11.84DD160 pKa = 3.1RR161 pKa = 11.84TFDD164 pKa = 3.44RR165 pKa = 11.84LDD167 pKa = 3.27YY168 pKa = 10.57STEE171 pKa = 3.89QGLKK175 pKa = 10.12ASQPTRR181 pKa = 11.84TTASGQMLTGSDD193 pKa = 3.48AVMEE197 pKa = 4.3SSGRR201 pKa = 11.84LNPAHH206 pKa = 6.53SRR208 pKa = 11.84WLMGYY213 pKa = 7.37PQEE216 pKa = 4.29WDD218 pKa = 3.5DD219 pKa = 4.33CAVTAMPSSRR229 pKa = 11.84KK230 pKa = 9.61

Molecular weight: 25.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12871

59

984

247.5

27.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.023 ± 0.427

1.01 ± 0.142

6.262 ± 0.253

6.511 ± 0.385

3.838 ± 0.138

7.668 ± 0.251

1.756 ± 0.193

5.081 ± 0.15

5.967 ± 0.411

7.746 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.148

4.126 ± 0.206

3.986 ± 0.241

3.714 ± 0.305

5.423 ± 0.253

6.239 ± 0.4

6.519 ± 0.419

6.985 ± 0.305

1.383 ± 0.181

3.426 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski