Amino acid dipepetide frequency for Escherichia phage K1G

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.189AlaAla: 11.189 ± 1.588
1.243AlaCys: 1.243 ± 0.376
6.605AlaAsp: 6.605 ± 0.75
6.061AlaGlu: 6.061 ± 0.802
4.74AlaPhe: 4.74 ± 0.552
7.071AlaGly: 7.071 ± 0.725
1.632AlaHis: 1.632 ± 0.434
5.128AlaIle: 5.128 ± 0.806
5.983AlaLys: 5.983 ± 0.736
9.091AlaLeu: 9.091 ± 0.878
1.709AlaMet: 1.709 ± 0.378
3.652AlaAsn: 3.652 ± 0.546
4.196AlaPro: 4.196 ± 0.531
3.263AlaGln: 3.263 ± 0.676
4.895AlaArg: 4.895 ± 0.562
5.828AlaSer: 5.828 ± 0.833
5.983AlaThr: 5.983 ± 0.861
6.838AlaVal: 6.838 ± 0.696
1.554AlaTrp: 1.554 ± 0.372
3.574AlaTyr: 3.574 ± 0.419
0.0AlaXaa: 0.0 ± 0.0
Cys
1.166CysAla: 1.166 ± 0.366
0.078CysCys: 0.078 ± 0.072
1.01CysAsp: 1.01 ± 0.279
0.777CysGlu: 0.777 ± 0.339
0.233CysPhe: 0.233 ± 0.121
1.01CysGly: 1.01 ± 0.255
0.233CysHis: 0.233 ± 0.135
0.389CysIle: 0.389 ± 0.21
0.777CysLys: 0.777 ± 0.246
0.466CysLeu: 0.466 ± 0.21
0.155CysMet: 0.155 ± 0.096
0.311CysAsn: 0.311 ± 0.211
0.233CysPro: 0.233 ± 0.156
0.311CysGln: 0.311 ± 0.161
0.622CysArg: 0.622 ± 0.24
0.466CysSer: 0.466 ± 0.199
0.466CysThr: 0.466 ± 0.249
0.777CysVal: 0.777 ± 0.247
0.155CysTrp: 0.155 ± 0.099
0.466CysTyr: 0.466 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
6.449AspAla: 6.449 ± 0.857
0.699AspCys: 0.699 ± 0.249
5.128AspAsp: 5.128 ± 0.686
4.74AspGlu: 4.74 ± 0.61
2.564AspPhe: 2.564 ± 0.358
6.294AspGly: 6.294 ± 1.006
0.544AspHis: 0.544 ± 0.225
4.196AspIle: 4.196 ± 0.446
3.186AspLys: 3.186 ± 0.437
4.895AspLeu: 4.895 ± 0.57
1.787AspMet: 1.787 ± 0.388
2.486AspAsn: 2.486 ± 0.456
1.787AspPro: 1.787 ± 0.332
0.855AspGln: 0.855 ± 0.233
2.486AspArg: 2.486 ± 0.519
2.953AspSer: 2.953 ± 0.472
3.652AspThr: 3.652 ± 0.487
4.507AspVal: 4.507 ± 0.496
0.855AspTrp: 0.855 ± 0.221
2.564AspTyr: 2.564 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
6.216GluAla: 6.216 ± 0.683
0.233GluCys: 0.233 ± 0.145
3.497GluAsp: 3.497 ± 0.486
4.817GluGlu: 4.817 ± 0.934
2.797GluPhe: 2.797 ± 0.477
4.584GluGly: 4.584 ± 0.775
0.777GluHis: 0.777 ± 0.229
3.341GluIle: 3.341 ± 0.551
4.351GluLys: 4.351 ± 0.703
5.75GluLeu: 5.75 ± 0.579
2.176GluMet: 2.176 ± 0.469
2.253GluAsn: 2.253 ± 0.516
1.787GluPro: 1.787 ± 0.405
3.263GluGln: 3.263 ± 0.836
3.885GluArg: 3.885 ± 0.55
3.03GluSer: 3.03 ± 0.477
3.574GluThr: 3.574 ± 0.593
4.74GluVal: 4.74 ± 0.566
1.399GluTrp: 1.399 ± 0.314
2.564GluTyr: 2.564 ± 0.366
0.0GluXaa: 0.0 ± 0.0
Phe
2.642PheAla: 2.642 ± 0.463
0.311PheCys: 0.311 ± 0.176
3.885PheAsp: 3.885 ± 0.516
2.797PheGlu: 2.797 ± 0.55
1.01PhePhe: 1.01 ± 0.267
3.419PheGly: 3.419 ± 0.497
0.466PheHis: 0.466 ± 0.206
2.642PheIle: 2.642 ± 0.456
2.642PheLys: 2.642 ± 0.473
2.098PheLeu: 2.098 ± 0.393
0.699PheMet: 0.699 ± 0.189
1.632PheAsn: 1.632 ± 0.342
1.088PhePro: 1.088 ± 0.313
1.243PheGln: 1.243 ± 0.274
2.02PheArg: 2.02 ± 0.297
2.875PheSer: 2.875 ± 0.464
2.564PheThr: 2.564 ± 0.346
2.486PheVal: 2.486 ± 0.405
0.622PheTrp: 0.622 ± 0.235
1.243PheTyr: 1.243 ± 0.298
0.0PheXaa: 0.0 ± 0.0
Gly
7.071GlyAla: 7.071 ± 0.844
1.088GlyCys: 1.088 ± 0.33
5.051GlyAsp: 5.051 ± 0.702
5.439GlyGlu: 5.439 ± 0.704
3.263GlyPhe: 3.263 ± 0.609
5.594GlyGly: 5.594 ± 0.75
1.709GlyHis: 1.709 ± 0.408
2.642GlyIle: 2.642 ± 0.473
5.439GlyLys: 5.439 ± 0.797
5.75GlyLeu: 5.75 ± 0.647
1.943GlyMet: 1.943 ± 0.513
3.574GlyAsn: 3.574 ± 0.7
1.787GlyPro: 1.787 ± 0.3
3.186GlyGln: 3.186 ± 0.554
3.497GlyArg: 3.497 ± 0.536
5.594GlySer: 5.594 ± 0.813
4.196GlyThr: 4.196 ± 0.447
5.594GlyVal: 5.594 ± 0.777
1.321GlyTrp: 1.321 ± 0.287
2.953GlyTyr: 2.953 ± 0.501
0.0GlyXaa: 0.0 ± 0.0
His
1.01HisAla: 1.01 ± 0.241
0.466HisCys: 0.466 ± 0.171
1.01HisAsp: 1.01 ± 0.253
1.01HisGlu: 1.01 ± 0.307
0.855HisPhe: 0.855 ± 0.262
1.399HisGly: 1.399 ± 0.307
0.932HisHis: 0.932 ± 0.247
0.855HisIle: 0.855 ± 0.241
1.243HisLys: 1.243 ± 0.369
1.243HisLeu: 1.243 ± 0.341
0.389HisMet: 0.389 ± 0.172
0.622HisAsn: 0.622 ± 0.196
1.01HisPro: 1.01 ± 0.314
0.544HisGln: 0.544 ± 0.179
1.321HisArg: 1.321 ± 0.295
0.855HisSer: 0.855 ± 0.245
0.699HisThr: 0.699 ± 0.26
1.554HisVal: 1.554 ± 0.391
0.078HisTrp: 0.078 ± 0.072
0.466HisTyr: 0.466 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.507IleAla: 4.507 ± 0.635
0.544IleCys: 0.544 ± 0.197
3.419IleAsp: 3.419 ± 0.509
3.574IleGlu: 3.574 ± 0.405
1.399IlePhe: 1.399 ± 0.341
2.564IleGly: 2.564 ± 0.381
0.699IleHis: 0.699 ± 0.277
2.72IleIle: 2.72 ± 0.35
3.341IleLys: 3.341 ± 0.528
3.497IleLeu: 3.497 ± 0.548
0.777IleMet: 0.777 ± 0.282
2.797IleAsn: 2.797 ± 0.44
3.03IlePro: 3.03 ± 0.39
1.709IleGln: 1.709 ± 0.427
1.865IleArg: 1.865 ± 0.336
3.885IleSer: 3.885 ± 0.585
4.429IleThr: 4.429 ± 0.614
3.73IleVal: 3.73 ± 0.426
0.855IleTrp: 0.855 ± 0.229
1.476IleTyr: 1.476 ± 0.395
0.0IleXaa: 0.0 ± 0.0
Lys
7.071LysAla: 7.071 ± 1.091
0.544LysCys: 0.544 ± 0.242
3.497LysAsp: 3.497 ± 0.631
4.584LysGlu: 4.584 ± 0.897
2.098LysPhe: 2.098 ± 0.419
3.652LysGly: 3.652 ± 0.49
1.554LysHis: 1.554 ± 0.321
2.098LysIle: 2.098 ± 0.408
3.574LysLys: 3.574 ± 0.615
4.74LysLeu: 4.74 ± 0.584
2.953LysMet: 2.953 ± 0.566
1.243LysAsn: 1.243 ± 0.287
2.331LysPro: 2.331 ± 0.39
2.176LysGln: 2.176 ± 0.517
3.807LysArg: 3.807 ± 0.556
3.108LysSer: 3.108 ± 0.466
4.196LysThr: 4.196 ± 0.534
3.497LysVal: 3.497 ± 0.733
0.544LysTrp: 0.544 ± 0.167
2.409LysTyr: 2.409 ± 0.385
0.0LysXaa: 0.0 ± 0.0
Leu
9.324LeuAla: 9.324 ± 0.898
0.777LeuCys: 0.777 ± 0.269
4.04LeuAsp: 4.04 ± 0.609
4.429LeuGlu: 4.429 ± 0.74
2.331LeuPhe: 2.331 ± 0.482
5.284LeuGly: 5.284 ± 0.488
1.399LeuHis: 1.399 ± 0.316
4.196LeuIle: 4.196 ± 0.417
4.351LeuLys: 4.351 ± 0.566
5.75LeuLeu: 5.75 ± 0.68
1.787LeuMet: 1.787 ± 0.32
3.963LeuAsn: 3.963 ± 0.698
3.73LeuPro: 3.73 ± 0.613
2.72LeuGln: 2.72 ± 0.469
4.662LeuArg: 4.662 ± 0.616
4.04LeuSer: 4.04 ± 0.552
6.527LeuThr: 6.527 ± 0.805
4.662LeuVal: 4.662 ± 0.678
1.01LeuTrp: 1.01 ± 0.331
2.486LeuTyr: 2.486 ± 0.527
0.0LeuXaa: 0.0 ± 0.0
Met
3.03MetAla: 3.03 ± 0.463
0.622MetCys: 0.622 ± 0.196
0.699MetAsp: 0.699 ± 0.294
1.088MetGlu: 1.088 ± 0.263
0.466MetPhe: 0.466 ± 0.207
2.253MetGly: 2.253 ± 0.332
0.311MetHis: 0.311 ± 0.155
1.399MetIle: 1.399 ± 0.362
1.787MetLys: 1.787 ± 0.412
1.632MetLeu: 1.632 ± 0.359
0.311MetMet: 0.311 ± 0.135
0.699MetAsn: 0.699 ± 0.226
1.321MetPro: 1.321 ± 0.302
0.544MetGln: 0.544 ± 0.204
1.166MetArg: 1.166 ± 0.209
2.409MetSer: 2.409 ± 0.323
1.632MetThr: 1.632 ± 0.337
1.476MetVal: 1.476 ± 0.348
0.155MetTrp: 0.155 ± 0.103
0.389MetTyr: 0.389 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
4.196AsnAla: 4.196 ± 0.417
0.389AsnCys: 0.389 ± 0.162
2.253AsnAsp: 2.253 ± 0.425
2.176AsnGlu: 2.176 ± 0.413
0.777AsnPhe: 0.777 ± 0.249
4.04AsnGly: 4.04 ± 0.615
0.622AsnHis: 0.622 ± 0.251
1.787AsnIle: 1.787 ± 0.359
1.709AsnLys: 1.709 ± 0.31
2.486AsnLeu: 2.486 ± 0.427
0.855AsnMet: 0.855 ± 0.266
2.176AsnAsn: 2.176 ± 0.375
2.253AsnPro: 2.253 ± 0.416
1.632AsnGln: 1.632 ± 0.409
2.176AsnArg: 2.176 ± 0.493
2.797AsnSer: 2.797 ± 0.381
2.875AsnThr: 2.875 ± 0.451
3.807AsnVal: 3.807 ± 0.477
0.699AsnTrp: 0.699 ± 0.198
1.554AsnTyr: 1.554 ± 0.321
0.0AsnXaa: 0.0 ± 0.0
Pro
3.497ProAla: 3.497 ± 0.599
0.389ProCys: 0.389 ± 0.157
2.875ProAsp: 2.875 ± 0.554
3.574ProGlu: 3.574 ± 0.506
2.098ProPhe: 2.098 ± 0.439
3.108ProGly: 3.108 ± 0.426
0.699ProHis: 0.699 ± 0.241
1.709ProIle: 1.709 ± 0.324
1.632ProLys: 1.632 ± 0.395
2.875ProLeu: 2.875 ± 0.54
0.855ProMet: 0.855 ± 0.287
1.787ProAsn: 1.787 ± 0.435
1.088ProPro: 1.088 ± 0.381
1.01ProGln: 1.01 ± 0.222
2.098ProArg: 2.098 ± 0.387
2.486ProSer: 2.486 ± 0.439
2.486ProThr: 2.486 ± 0.503
4.04ProVal: 4.04 ± 0.606
0.389ProTrp: 0.389 ± 0.188
1.166ProTyr: 1.166 ± 0.288
0.0ProXaa: 0.0 ± 0.0
Gln
3.963GlnAla: 3.963 ± 0.629
0.466GlnCys: 0.466 ± 0.248
2.02GlnAsp: 2.02 ± 0.416
2.176GlnGlu: 2.176 ± 0.441
1.243GlnPhe: 1.243 ± 0.237
1.709GlnGly: 1.709 ± 0.331
0.699GlnHis: 0.699 ± 0.212
1.943GlnIle: 1.943 ± 0.364
2.253GlnLys: 2.253 ± 0.523
3.263GlnLeu: 3.263 ± 0.383
1.01GlnMet: 1.01 ± 0.229
1.554GlnAsn: 1.554 ± 0.387
1.787GlnPro: 1.787 ± 0.346
2.253GlnGln: 2.253 ± 0.682
2.409GlnArg: 2.409 ± 0.427
1.865GlnSer: 1.865 ± 0.398
1.943GlnThr: 1.943 ± 0.447
2.564GlnVal: 2.564 ± 0.463
0.855GlnTrp: 0.855 ± 0.221
1.166GlnTyr: 1.166 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
4.351ArgAla: 4.351 ± 0.484
0.466ArgCys: 0.466 ± 0.159
2.409ArgAsp: 2.409 ± 0.41
3.419ArgGlu: 3.419 ± 0.537
2.176ArgPhe: 2.176 ± 0.394
3.807ArgGly: 3.807 ± 0.479
1.243ArgHis: 1.243 ± 0.308
2.875ArgIle: 2.875 ± 0.47
4.507ArgLys: 4.507 ± 0.766
4.662ArgLeu: 4.662 ± 0.646
1.321ArgMet: 1.321 ± 0.318
2.797ArgAsn: 2.797 ± 0.472
1.554ArgPro: 1.554 ± 0.364
2.953ArgGln: 2.953 ± 0.44
4.351ArgArg: 4.351 ± 0.705
3.341ArgSer: 3.341 ± 0.364
2.72ArgThr: 2.72 ± 0.517
3.341ArgVal: 3.341 ± 0.471
0.699ArgTrp: 0.699 ± 0.213
1.243ArgTyr: 1.243 ± 0.26
0.0ArgXaa: 0.0 ± 0.0
Ser
5.361SerAla: 5.361 ± 0.592
0.233SerCys: 0.233 ± 0.113
4.196SerAsp: 4.196 ± 0.399
2.564SerGlu: 2.564 ± 0.44
2.642SerPhe: 2.642 ± 0.461
6.76SerGly: 6.76 ± 0.684
0.932SerHis: 0.932 ± 0.249
2.875SerIle: 2.875 ± 0.697
3.341SerLys: 3.341 ± 0.561
4.74SerLeu: 4.74 ± 0.523
1.399SerMet: 1.399 ± 0.344
2.486SerAsn: 2.486 ± 0.547
3.03SerPro: 3.03 ± 0.528
2.02SerGln: 2.02 ± 0.525
3.807SerArg: 3.807 ± 0.634
4.351SerSer: 4.351 ± 0.952
4.584SerThr: 4.584 ± 0.642
4.507SerVal: 4.507 ± 0.545
0.389SerTrp: 0.389 ± 0.145
2.176SerTyr: 2.176 ± 0.53
0.0SerXaa: 0.0 ± 0.0
Thr
7.537ThrAla: 7.537 ± 0.99
0.466ThrCys: 0.466 ± 0.186
3.885ThrAsp: 3.885 ± 0.481
4.507ThrGlu: 4.507 ± 0.545
3.03ThrPhe: 3.03 ± 0.527
5.983ThrGly: 5.983 ± 0.904
1.01ThrHis: 1.01 ± 0.306
3.497ThrIle: 3.497 ± 0.502
3.497ThrLys: 3.497 ± 0.426
4.973ThrLeu: 4.973 ± 0.482
1.166ThrMet: 1.166 ± 0.298
1.632ThrAsn: 1.632 ± 0.408
4.274ThrPro: 4.274 ± 0.61
2.02ThrGln: 2.02 ± 0.398
2.564ThrArg: 2.564 ± 0.325
3.341ThrSer: 3.341 ± 0.529
4.04ThrThr: 4.04 ± 0.669
4.429ThrVal: 4.429 ± 0.511
0.855ThrTrp: 0.855 ± 0.272
2.486ThrTyr: 2.486 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
7.382ValAla: 7.382 ± 0.692
0.544ValCys: 0.544 ± 0.2
3.807ValAsp: 3.807 ± 0.488
4.74ValGlu: 4.74 ± 0.873
2.02ValPhe: 2.02 ± 0.346
4.662ValGly: 4.662 ± 0.485
0.932ValHis: 0.932 ± 0.225
4.817ValIle: 4.817 ± 0.745
3.497ValLys: 3.497 ± 0.529
5.051ValLeu: 5.051 ± 0.639
0.932ValMet: 0.932 ± 0.214
3.419ValAsn: 3.419 ± 0.613
1.865ValPro: 1.865 ± 0.491
2.875ValGln: 2.875 ± 0.487
3.807ValArg: 3.807 ± 0.533
5.517ValSer: 5.517 ± 0.633
5.517ValThr: 5.517 ± 0.763
4.973ValVal: 4.973 ± 0.743
1.088ValTrp: 1.088 ± 0.334
2.409ValTyr: 2.409 ± 0.502
0.0ValXaa: 0.0 ± 0.0
Trp
1.088TrpAla: 1.088 ± 0.433
0.078TrpCys: 0.078 ± 0.071
0.855TrpAsp: 0.855 ± 0.197
0.622TrpGlu: 0.622 ± 0.256
0.855TrpPhe: 0.855 ± 0.283
0.699TrpGly: 0.699 ± 0.275
0.466TrpHis: 0.466 ± 0.191
0.078TrpIle: 0.078 ± 0.059
0.699TrpLys: 0.699 ± 0.209
2.02TrpLeu: 2.02 ± 0.301
0.544TrpMet: 0.544 ± 0.201
0.699TrpAsn: 0.699 ± 0.216
0.544TrpPro: 0.544 ± 0.208
0.699TrpGln: 0.699 ± 0.275
0.699TrpArg: 0.699 ± 0.263
1.321TrpSer: 1.321 ± 0.381
0.622TrpThr: 0.622 ± 0.188
0.699TrpVal: 0.699 ± 0.257
0.078TrpTrp: 0.078 ± 0.08
0.466TrpTyr: 0.466 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.186TyrAla: 3.186 ± 0.529
0.466TyrCys: 0.466 ± 0.174
2.486TyrAsp: 2.486 ± 0.652
2.02TyrGlu: 2.02 ± 0.307
1.787TyrPhe: 1.787 ± 0.347
2.797TyrGly: 2.797 ± 0.406
0.699TyrHis: 0.699 ± 0.217
1.632TyrIle: 1.632 ± 0.331
1.865TyrLys: 1.865 ± 0.328
2.486TyrLeu: 2.486 ± 0.368
0.544TyrMet: 0.544 ± 0.157
1.476TyrAsn: 1.476 ± 0.306
1.166TyrPro: 1.166 ± 0.454
1.865TyrGln: 1.865 ± 0.305
2.098TyrArg: 2.098 ± 0.431
2.331TyrSer: 2.331 ± 0.403
2.486TyrThr: 2.486 ± 0.459
1.632TyrVal: 1.632 ± 0.343
0.233TyrTrp: 0.233 ± 0.123
1.01TyrTyr: 1.01 ± 0.22
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski