Erwinia phage vB_EamP-L1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Elunavirus; Erwinia virus L1

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0YQ77|G0YQ77_9CAUD Gp13.5 OS=Erwinia phage vB_EamP-L1 OX=1051673 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.39SFLNPADD10 pKa = 4.05EE11 pKa = 5.14LDD13 pKa = 4.69AINDD17 pKa = 3.39ILASIGEE24 pKa = 4.38APVLTLGDD32 pKa = 3.93SANVDD37 pKa = 3.18VSNAQQILSKK47 pKa = 9.54VNRR50 pKa = 11.84MIQSKK55 pKa = 9.52GWTFNIEE62 pKa = 3.77EE63 pKa = 4.53GAVLVPDD70 pKa = 4.25VNTGLIPYY78 pKa = 8.62LPSYY82 pKa = 10.74LRR84 pKa = 11.84VQGADD89 pKa = 3.26SPNTYY94 pKa = 10.03INRR97 pKa = 11.84QGYY100 pKa = 9.53VYY102 pKa = 9.51DD103 pKa = 3.84TAGLTDD109 pKa = 3.78VFQGSISVNLTRR121 pKa = 11.84LRR123 pKa = 11.84EE124 pKa = 4.0YY125 pKa = 11.23SEE127 pKa = 4.23MPQCFRR133 pKa = 11.84SYY135 pKa = 10.7IVTKK139 pKa = 10.66ASRR142 pKa = 11.84QFNMRR147 pKa = 11.84YY148 pKa = 9.83FGDD151 pKa = 3.7TAIEE155 pKa = 4.4SILAEE160 pKa = 4.33EE161 pKa = 4.04EE162 pKa = 3.99AEE164 pKa = 6.0AYY166 pKa = 7.38MQCNEE171 pKa = 4.04YY172 pKa = 10.61EE173 pKa = 3.91LDD175 pKa = 3.59YY176 pKa = 11.67GKK178 pKa = 11.11FNMLDD183 pKa = 2.89GDD185 pKa = 4.29AFVQGILSRR194 pKa = 3.97

Molecular weight: 21.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0YQ52|G0YQ52_9CAUD Gp1.6 OS=Erwinia phage vB_EamP-L1 OX=1051673 PE=4 SV=1
MM1 pKa = 7.45EE2 pKa = 6.3AYY4 pKa = 7.73DD5 pKa = 4.06TQPSASSEE13 pKa = 4.03EE14 pKa = 4.04LRR16 pKa = 11.84LIPLRR21 pKa = 11.84KK22 pKa = 9.42YY23 pKa = 10.25KK24 pKa = 10.58YY25 pKa = 10.28NPASPSGLIFARR37 pKa = 11.84SGKK40 pKa = 9.6PCGTLRR46 pKa = 11.84KK47 pKa = 9.66DD48 pKa = 4.55GYY50 pKa = 10.49WSTQSRR56 pKa = 11.84KK57 pKa = 10.14KK58 pKa = 10.33NLQVHH63 pKa = 5.7RR64 pKa = 11.84LVWIANFGAIPPGMEE79 pKa = 3.81IDD81 pKa = 4.36HH82 pKa = 7.24IDD84 pKa = 3.62RR85 pKa = 11.84NPSNNLIEE93 pKa = 4.11NLRR96 pKa = 11.84LVSRR100 pKa = 11.84SQNGFNRR107 pKa = 11.84RR108 pKa = 11.84VPRR111 pKa = 11.84QSQTGQRR118 pKa = 11.84YY119 pKa = 7.8IHH121 pKa = 6.09TCRR124 pKa = 11.84KK125 pKa = 7.54TGNFTVRR132 pKa = 11.84IARR135 pKa = 11.84VSYY138 pKa = 8.41GTFPTLEE145 pKa = 3.97EE146 pKa = 4.04ALEE149 pKa = 4.16VRR151 pKa = 11.84DD152 pKa = 4.55SLIKK156 pKa = 9.2THH158 pKa = 6.55

Molecular weight: 18.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13043

33

1331

255.7

28.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.668 ± 0.518

0.997 ± 0.171

6.463 ± 0.29

6.272 ± 0.342

3.718 ± 0.198

7.92 ± 0.319

1.809 ± 0.168

4.999 ± 0.178

6.325 ± 0.338

7.997 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.929 ± 0.2

4.293 ± 0.172

3.665 ± 0.164

4.47 ± 0.294

5.428 ± 0.173

6.149 ± 0.338

5.727 ± 0.262

6.709 ± 0.297

1.388 ± 0.182

3.074 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski