Mastigocladus laminosus WC112

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Hapalosiphonaceae; Mastigocladus; Mastigocladus laminosus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 954 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370CM65|A0A370CM65_MASLA Robl_LC7 domain-containing protein OS=Mastigocladus laminosus WC112 OX=1521402 GN=CBF18_00280 PE=4 SV=1
MM1 pKa = 6.94TVRR4 pKa = 11.84VHH6 pKa = 6.89FLPDD10 pKa = 3.72DD11 pKa = 3.87VTVDD15 pKa = 3.59AQVGEE20 pKa = 4.33PLLDD24 pKa = 3.37VADD27 pKa = 4.33RR28 pKa = 11.84AEE30 pKa = 4.26VIIPTGCLMGTCHH43 pKa = 7.06ACTVEE48 pKa = 4.51LDD50 pKa = 3.48DD51 pKa = 5.46GEE53 pKa = 4.71IIRR56 pKa = 11.84ACISSVPDD64 pKa = 3.29GCEE67 pKa = 3.39EE68 pKa = 3.79LTINLFSDD76 pKa = 4.03PTWW79 pKa = 4.29

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370CK77|A0A370CK77_MASLA Uncharacterized protein OS=Mastigocladus laminosus WC112 OX=1521402 GN=CBF18_20895 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.03VFVSSKK8 pKa = 10.27KK9 pKa = 10.92GNGLTTNALFALAYY23 pKa = 10.23LAGGDD28 pKa = 4.42CIANARR34 pKa = 11.84PGSFLDD40 pKa = 3.52AFFFSVQTLASIGYY54 pKa = 9.0GAMFPKK60 pKa = 8.22TTYY63 pKa = 10.75ANVVVTIEE71 pKa = 3.9AMMGLVGIAVLTGLAFARR89 pKa = 11.84FSRR92 pKa = 11.84PTARR96 pKa = 11.84VIFSRR101 pKa = 11.84VAVIAPHH108 pKa = 6.51NDD110 pKa = 2.52VPTLMFRR117 pKa = 11.84AANKK121 pKa = 9.66RR122 pKa = 11.84RR123 pKa = 11.84NQILEE128 pKa = 3.91AQLRR132 pKa = 11.84VYY134 pKa = 11.02LMRR137 pKa = 11.84KK138 pKa = 8.71

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

954

0

954

129866

9

715

136.1

15.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.551 ± 0.114

1.208 ± 0.038

4.564 ± 0.085

6.246 ± 0.108

4.035 ± 0.066

6.032 ± 0.099

1.85 ± 0.046

7.073 ± 0.09

5.297 ± 0.09

11.063 ± 0.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.048

4.445 ± 0.076

4.491 ± 0.073

5.433 ± 0.083

5.121 ± 0.078

6.306 ± 0.074

5.582 ± 0.07

6.647 ± 0.098

1.487 ± 0.044

3.246 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski