Crenobacter sp. GY 70310
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4T0V0K5|A0A4T0V0K5_9NEIS Phosphate transport system permease protein OS=Crenobacter sp. GY 70310 OX=2563443 GN=pstC PE=3 SV=1
MM1 pKa = 7.21 TATTEE6 pKa = 4.1 MPSPINFTDD15 pKa = 3.72 SACDD19 pKa = 3.4 KK20 pKa = 10.54 VRR22 pKa = 11.84 EE23 pKa = 4.12 LVAEE27 pKa = 4.28 EE28 pKa = 4.56 GNPEE32 pKa = 3.75 LKK34 pKa = 10.69 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 5.2 EE55 pKa = 4.78 IVNDD59 pKa = 4.08 DD60 pKa = 3.41 DD61 pKa = 4.23 TAIEE65 pKa = 4.07 RR66 pKa = 11.84 AGVTFLVDD74 pKa = 3.5 PMSYY78 pKa = 10.19 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.99 IDD87 pKa = 4.0 YY88 pKa = 11.04 QDD90 pKa = 4.26 SLEE93 pKa = 4.25 GSQFVIRR100 pKa = 11.84 NPNATTTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.63
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.948
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A4T0UPB7|A0A4T0UPB7_9NEIS Energy transducer TonB OS=Crenobacter sp. GY 70310 OX=2563443 GN=E5K04_12195 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSTTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.24 RR14 pKa = 11.84 THH16 pKa = 5.92 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.68 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3190
0
3190
1028072
24
2126
322.3
34.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.401 ± 0.069
1.076 ± 0.015
5.292 ± 0.031
5.576 ± 0.038
3.614 ± 0.029
8.44 ± 0.046
2.137 ± 0.022
4.122 ± 0.035
3.255 ± 0.039
11.507 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.388 ± 0.023
2.502 ± 0.029
4.886 ± 0.033
3.601 ± 0.031
6.899 ± 0.045
5.111 ± 0.033
4.671 ± 0.028
7.672 ± 0.038
1.44 ± 0.018
2.408 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here