Neobacillus mesonae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Neobacillus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R198|A0A3Q9R198_9BACI Peptidylprolyl isomerase OS=Neobacillus mesonae OX=1193713 GN=CHR53_23950 PE=4 SV=1
MM1 pKa = 7.12QSFVVSFHH9 pKa = 7.07QEE11 pKa = 4.01DD12 pKa = 4.02NVDD15 pKa = 3.08TMQIQKK21 pKa = 10.57LSQEE25 pKa = 4.7DD26 pKa = 3.46FDD28 pKa = 4.64KK29 pKa = 10.88ATEE32 pKa = 4.3GGTRR36 pKa = 11.84HH37 pKa = 6.61LFEE40 pKa = 6.96LDD42 pKa = 3.3TNIGFFVFFDD52 pKa = 4.09GADD55 pKa = 3.42KK56 pKa = 11.38DD57 pKa = 4.31GDD59 pKa = 3.5ISYY62 pKa = 10.52MMLQYY67 pKa = 10.94EE68 pKa = 4.65EE69 pKa = 5.77DD70 pKa = 3.7NEE72 pKa = 4.29EE73 pKa = 4.1PSACYY78 pKa = 10.11AFQLSDD84 pKa = 3.72FYY86 pKa = 11.61EE87 pKa = 5.02FMALYY92 pKa = 10.93LNDD95 pKa = 4.23LEE97 pKa = 4.69FNEE100 pKa = 4.42EE101 pKa = 3.96VEE103 pKa = 4.25EE104 pKa = 4.69EE105 pKa = 4.01EE106 pKa = 4.53DD107 pKa = 3.58EE108 pKa = 4.61EE109 pKa = 4.48YY110 pKa = 11.52GPIHH114 pKa = 6.63HH115 pKa = 7.18LAHH118 pKa = 6.77LLFHH122 pKa = 7.06IIEE125 pKa = 4.39EE126 pKa = 4.49GRR128 pKa = 11.84DD129 pKa = 3.57LEE131 pKa = 4.54VV132 pKa = 3.67

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9QYV7|A0A3Q9QYV7_9BACI C-terminal binding protein OS=Neobacillus mesonae OX=1193713 GN=CHR53_12315 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSSNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5368

0

5368

1607557

30

1948

299.5

33.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.251 ± 0.039

0.757 ± 0.011

4.872 ± 0.028

7.095 ± 0.036

4.678 ± 0.027

7.164 ± 0.033

2.019 ± 0.015

7.963 ± 0.037

7.14 ± 0.033

9.705 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.017

4.481 ± 0.027

3.741 ± 0.018

3.643 ± 0.023

3.932 ± 0.022

5.898 ± 0.021

5.379 ± 0.025

6.965 ± 0.028

1.074 ± 0.014

3.511 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski