Amino acid dipepetide frequency for Neobacillus mesonae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.07AlaAla: 7.07 ± 0.083
0.625AlaCys: 0.625 ± 0.022
3.487AlaAsp: 3.487 ± 0.05
4.808AlaGlu: 4.808 ± 0.062
3.325AlaPhe: 3.325 ± 0.049
5.832AlaGly: 5.832 ± 0.073
1.277AlaHis: 1.277 ± 0.032
5.994AlaIle: 5.994 ± 0.066
5.003AlaLys: 5.003 ± 0.058
7.115AlaLeu: 7.115 ± 0.067
2.007AlaMet: 2.007 ± 0.041
2.939AlaAsn: 2.939 ± 0.047
2.238AlaPro: 2.238 ± 0.049
2.188AlaGln: 2.188 ± 0.04
2.617AlaArg: 2.617 ± 0.044
4.108AlaSer: 4.108 ± 0.061
3.138AlaThr: 3.138 ± 0.047
5.811AlaVal: 5.811 ± 0.06
0.638AlaTrp: 0.638 ± 0.021
2.294AlaTyr: 2.294 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.492CysAla: 0.492 ± 0.016
0.099CysCys: 0.099 ± 0.008
0.386CysAsp: 0.386 ± 0.015
0.445CysGlu: 0.445 ± 0.019
0.354CysPhe: 0.354 ± 0.016
0.756CysGly: 0.756 ± 0.029
0.231CysHis: 0.231 ± 0.015
0.562CysIle: 0.562 ± 0.018
0.393CysLys: 0.393 ± 0.017
0.706CysLeu: 0.706 ± 0.021
0.187CysMet: 0.187 ± 0.011
0.274CysAsn: 0.274 ± 0.011
0.369CysPro: 0.369 ± 0.017
0.265CysGln: 0.265 ± 0.014
0.319CysArg: 0.319 ± 0.015
0.541CysSer: 0.541 ± 0.02
0.421CysThr: 0.421 ± 0.016
0.417CysVal: 0.417 ± 0.018
0.085CysTrp: 0.085 ± 0.007
0.268CysTyr: 0.268 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.214AspAla: 3.214 ± 0.054
0.416AspCys: 0.416 ± 0.016
2.253AspAsp: 2.253 ± 0.039
3.865AspGlu: 3.865 ± 0.05
2.46AspPhe: 2.46 ± 0.044
3.4AspGly: 3.4 ± 0.061
1.16AspHis: 1.16 ± 0.028
3.818AspIle: 3.818 ± 0.053
3.061AspLys: 3.061 ± 0.054
4.924AspLeu: 4.924 ± 0.059
1.219AspMet: 1.219 ± 0.027
1.706AspAsn: 1.706 ± 0.036
1.986AspPro: 1.986 ± 0.04
2.031AspGln: 2.031 ± 0.035
2.141AspArg: 2.141 ± 0.039
2.464AspSer: 2.464 ± 0.047
2.297AspThr: 2.297 ± 0.044
3.591AspVal: 3.591 ± 0.051
0.656AspTrp: 0.656 ± 0.023
2.062AspTyr: 2.062 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.121GluAla: 5.121 ± 0.066
0.403GluCys: 0.403 ± 0.016
3.28GluAsp: 3.28 ± 0.052
6.167GluGlu: 6.167 ± 0.076
2.706GluPhe: 2.706 ± 0.044
4.123GluGly: 4.123 ± 0.058
1.379GluHis: 1.379 ± 0.031
5.676GluIle: 5.676 ± 0.07
6.551GluLys: 6.551 ± 0.071
6.852GluLeu: 6.852 ± 0.07
2.244GluMet: 2.244 ± 0.038
3.494GluAsn: 3.494 ± 0.045
1.955GluPro: 1.955 ± 0.036
3.046GluGln: 3.046 ± 0.041
3.202GluArg: 3.202 ± 0.048
3.258GluSer: 3.258 ± 0.046
3.791GluThr: 3.791 ± 0.05
4.642GluVal: 4.642 ± 0.059
0.816GluTrp: 0.816 ± 0.023
2.247GluTyr: 2.247 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
3.208PheAla: 3.208 ± 0.056
0.394PheCys: 0.394 ± 0.017
2.338PheAsp: 2.338 ± 0.042
2.775PheGlu: 2.775 ± 0.045
2.431PhePhe: 2.431 ± 0.049
3.513PheGly: 3.513 ± 0.051
1.04PheHis: 1.04 ± 0.026
4.019PheIle: 4.019 ± 0.065
2.61PheLys: 2.61 ± 0.046
4.742PheLeu: 4.742 ± 0.069
1.138PheMet: 1.138 ± 0.026
2.026PheAsn: 2.026 ± 0.039
1.805PhePro: 1.805 ± 0.035
1.644PheGln: 1.644 ± 0.034
1.605PheArg: 1.605 ± 0.033
3.345PheSer: 3.345 ± 0.05
2.662PheThr: 2.662 ± 0.04
3.186PheVal: 3.186 ± 0.048
0.526PheTrp: 0.526 ± 0.02
1.772PheTyr: 1.772 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.149GlyAla: 5.149 ± 0.059
0.671GlyCys: 0.671 ± 0.025
3.127GlyAsp: 3.127 ± 0.046
4.423GlyGlu: 4.423 ± 0.049
3.657GlyPhe: 3.657 ± 0.049
5.202GlyGly: 5.202 ± 0.08
1.4GlyHis: 1.4 ± 0.035
6.255GlyIle: 6.255 ± 0.079
5.584GlyLys: 5.584 ± 0.065
6.799GlyLeu: 6.799 ± 0.075
2.164GlyMet: 2.164 ± 0.042
2.931GlyAsn: 2.931 ± 0.058
1.904GlyPro: 1.904 ± 0.035
2.259GlyGln: 2.259 ± 0.042
2.733GlyArg: 2.733 ± 0.043
4.155GlySer: 4.155 ± 0.059
4.248GlyThr: 4.248 ± 0.051
5.222GlyVal: 5.222 ± 0.062
0.893GlyTrp: 0.893 ± 0.026
2.866GlyTyr: 2.866 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.199HisAla: 1.199 ± 0.029
0.19HisCys: 0.19 ± 0.011
1.02HisAsp: 1.02 ± 0.025
1.337HisGlu: 1.337 ± 0.026
1.071HisPhe: 1.071 ± 0.025
1.42HisGly: 1.42 ± 0.035
0.64HisHis: 0.64 ± 0.023
1.421HisIle: 1.421 ± 0.031
1.076HisLys: 1.076 ± 0.028
2.04HisLeu: 2.04 ± 0.035
0.496HisMet: 0.496 ± 0.021
0.753HisAsn: 0.753 ± 0.024
1.108HisPro: 1.108 ± 0.023
0.843HisGln: 0.843 ± 0.021
0.852HisArg: 0.852 ± 0.022
1.214HisSer: 1.214 ± 0.028
1.015HisThr: 1.015 ± 0.025
1.381HisVal: 1.381 ± 0.028
0.225HisTrp: 0.225 ± 0.011
0.885HisTyr: 0.885 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.122IleAla: 6.122 ± 0.066
0.716IleCys: 0.716 ± 0.022
4.103IleAsp: 4.103 ± 0.055
5.419IleGlu: 5.419 ± 0.061
3.515IlePhe: 3.515 ± 0.057
6.136IleGly: 6.136 ± 0.081
1.665IleHis: 1.665 ± 0.033
6.346IleIle: 6.346 ± 0.078
4.876IleLys: 4.876 ± 0.057
7.404IleLeu: 7.404 ± 0.085
1.915IleMet: 1.915 ± 0.04
3.473IleAsn: 3.473 ± 0.046
3.613IlePro: 3.613 ± 0.052
2.807IleGln: 2.807 ± 0.044
3.077IleArg: 3.077 ± 0.046
5.25IleSer: 5.25 ± 0.066
4.401IleThr: 4.401 ± 0.05
5.564IleVal: 5.564 ± 0.062
0.718IleTrp: 0.718 ± 0.025
2.508IleTyr: 2.508 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
5.135LysAla: 5.135 ± 0.062
0.345LysCys: 0.345 ± 0.015
3.862LysAsp: 3.862 ± 0.058
6.463LysGlu: 6.463 ± 0.074
2.14LysPhe: 2.14 ± 0.043
4.814LysGly: 4.814 ± 0.064
1.278LysHis: 1.278 ± 0.029
5.021LysIle: 5.021 ± 0.057
5.99LysLys: 5.99 ± 0.077
6.205LysLeu: 6.205 ± 0.065
2.237LysMet: 2.237 ± 0.04
3.635LysAsn: 3.635 ± 0.047
2.41LysPro: 2.41 ± 0.038
3.127LysGln: 3.127 ± 0.044
3.194LysArg: 3.194 ± 0.045
3.574LysSer: 3.574 ± 0.049
3.862LysThr: 3.862 ± 0.054
4.891LysVal: 4.891 ± 0.057
0.872LysTrp: 0.872 ± 0.026
2.34LysTyr: 2.34 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
7.424LeuAla: 7.424 ± 0.077
0.672LeuCys: 0.672 ± 0.017
4.565LeuAsp: 4.565 ± 0.056
6.513LeuGlu: 6.513 ± 0.066
4.904LeuPhe: 4.904 ± 0.073
6.621LeuGly: 6.621 ± 0.085
1.838LeuHis: 1.838 ± 0.035
7.452LeuIle: 7.452 ± 0.088
6.884LeuLys: 6.884 ± 0.07
9.957LeuLeu: 9.957 ± 0.11
2.537LeuMet: 2.537 ± 0.044
4.36LeuAsn: 4.36 ± 0.053
3.922LeuPro: 3.922 ± 0.046
3.451LeuGln: 3.451 ± 0.047
3.545LeuArg: 3.545 ± 0.052
6.53LeuSer: 6.53 ± 0.065
5.821LeuThr: 5.821 ± 0.061
6.17LeuVal: 6.17 ± 0.074
0.829LeuTrp: 0.829 ± 0.026
3.057LeuTyr: 3.057 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.161MetAla: 2.161 ± 0.038
0.157MetCys: 0.157 ± 0.011
1.441MetAsp: 1.441 ± 0.033
2.008MetGlu: 2.008 ± 0.033
1.107MetPhe: 1.107 ± 0.027
1.892MetGly: 1.892 ± 0.039
0.406MetHis: 0.406 ± 0.016
2.107MetIle: 2.107 ± 0.041
2.425MetLys: 2.425 ± 0.036
2.407MetLeu: 2.407 ± 0.045
0.84MetMet: 0.84 ± 0.024
1.504MetAsn: 1.504 ± 0.035
0.998MetPro: 0.998 ± 0.024
0.85MetGln: 0.85 ± 0.022
1.037MetArg: 1.037 ± 0.024
1.559MetSer: 1.559 ± 0.031
1.626MetThr: 1.626 ± 0.031
1.878MetVal: 1.878 ± 0.035
0.198MetTrp: 0.198 ± 0.011
0.709MetTyr: 0.709 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.86AsnAla: 2.86 ± 0.047
0.35AsnCys: 0.35 ± 0.015
2.175AsnAsp: 2.175 ± 0.042
3.215AsnGlu: 3.215 ± 0.044
1.727AsnPhe: 1.727 ± 0.033
3.62AsnGly: 3.62 ± 0.057
1.038AsnHis: 1.038 ± 0.025
3.459AsnIle: 3.459 ± 0.052
3.052AsnLys: 3.052 ± 0.045
3.97AsnLeu: 3.97 ± 0.052
1.183AsnMet: 1.183 ± 0.026
2.087AsnAsn: 2.087 ± 0.043
2.345AsnPro: 2.345 ± 0.038
1.952AsnGln: 1.952 ± 0.041
2.028AsnArg: 2.028 ± 0.033
2.442AsnSer: 2.442 ± 0.045
2.188AsnThr: 2.188 ± 0.04
2.973AsnVal: 2.973 ± 0.046
0.569AsnTrp: 0.569 ± 0.021
1.581AsnTyr: 1.581 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.606ProAla: 2.606 ± 0.043
0.22ProCys: 0.22 ± 0.012
2.038ProAsp: 2.038 ± 0.037
3.076ProGlu: 3.076 ± 0.049
2.073ProPhe: 2.073 ± 0.041
2.592ProGly: 2.592 ± 0.044
0.801ProHis: 0.801 ± 0.022
2.967ProIle: 2.967 ± 0.048
2.481ProLys: 2.481 ± 0.047
3.587ProLeu: 3.587 ± 0.048
0.878ProMet: 0.878 ± 0.022
1.808ProAsn: 1.808 ± 0.028
1.059ProPro: 1.059 ± 0.027
1.153ProGln: 1.153 ± 0.03
1.148ProArg: 1.148 ± 0.028
2.289ProSer: 2.289 ± 0.038
1.885ProThr: 1.885 ± 0.036
2.901ProVal: 2.901 ± 0.041
0.406ProTrp: 0.406 ± 0.016
1.444ProTyr: 1.444 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.712GlnAla: 2.712 ± 0.047
0.204GlnCys: 0.204 ± 0.012
1.566GlnAsp: 1.566 ± 0.035
2.748GlnGlu: 2.748 ± 0.046
1.668GlnPhe: 1.668 ± 0.031
2.167GlnGly: 2.167 ± 0.039
0.72GlnHis: 0.72 ± 0.02
2.679GlnIle: 2.679 ± 0.037
2.793GlnLys: 2.793 ± 0.053
3.864GlnLeu: 3.864 ± 0.048
1.139GlnMet: 1.139 ± 0.029
1.714GlnAsn: 1.714 ± 0.031
1.255GlnPro: 1.255 ± 0.03
1.716GlnGln: 1.716 ± 0.043
1.436GlnArg: 1.436 ± 0.034
1.984GlnSer: 1.984 ± 0.034
1.974GlnThr: 1.974 ± 0.035
2.311GlnVal: 2.311 ± 0.039
0.41GlnTrp: 0.41 ± 0.014
1.368GlnTyr: 1.368 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
2.361ArgAla: 2.361 ± 0.041
0.279ArgCys: 0.279 ± 0.017
1.954ArgAsp: 1.954 ± 0.04
3.139ArgGlu: 3.139 ± 0.053
1.895ArgPhe: 1.895 ± 0.035
2.397ArgGly: 2.397 ± 0.038
0.79ArgHis: 0.79 ± 0.024
3.082ArgIle: 3.082 ± 0.053
3.176ArgLys: 3.176 ± 0.045
3.94ArgLeu: 3.94 ± 0.054
1.199ArgMet: 1.199 ± 0.025
1.874ArgAsn: 1.874 ± 0.035
1.37ArgPro: 1.37 ± 0.031
1.463ArgGln: 1.463 ± 0.031
1.788ArgArg: 1.788 ± 0.036
2.101ArgSer: 2.101 ± 0.04
2.053ArgThr: 2.053 ± 0.036
2.59ArgVal: 2.59 ± 0.045
0.401ArgTrp: 0.401 ± 0.019
1.462ArgTyr: 1.462 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
3.839SerAla: 3.839 ± 0.049
0.434SerCys: 0.434 ± 0.016
2.635SerAsp: 2.635 ± 0.041
3.683SerGlu: 3.683 ± 0.051
3.319SerPhe: 3.319 ± 0.047
4.722SerGly: 4.722 ± 0.063
1.168SerHis: 1.168 ± 0.029
4.986SerIle: 4.986 ± 0.063
3.943SerLys: 3.943 ± 0.052
6.069SerLeu: 6.069 ± 0.065
1.64SerMet: 1.64 ± 0.036
2.449SerAsn: 2.449 ± 0.042
2.198SerPro: 2.198 ± 0.041
1.959SerGln: 1.959 ± 0.034
2.229SerArg: 2.229 ± 0.039
3.83SerSer: 3.83 ± 0.062
3.051SerThr: 3.051 ± 0.045
3.954SerVal: 3.954 ± 0.049
0.669SerTrp: 0.669 ± 0.022
2.202SerTyr: 2.202 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
4.369ThrAla: 4.369 ± 0.058
0.375ThrCys: 0.375 ± 0.015
2.624ThrAsp: 2.624 ± 0.046
3.229ThrGlu: 3.229 ± 0.046
2.646ThrPhe: 2.646 ± 0.044
4.448ThrGly: 4.448 ± 0.058
1.01ThrHis: 1.01 ± 0.025
4.637ThrIle: 4.637 ± 0.051
3.537ThrLys: 3.537 ± 0.052
5.004ThrLeu: 5.004 ± 0.061
1.247ThrMet: 1.247 ± 0.027
2.497ThrAsn: 2.497 ± 0.044
2.264ThrPro: 2.264 ± 0.038
1.413ThrGln: 1.413 ± 0.031
1.763ThrArg: 1.763 ± 0.036
3.089ThrSer: 3.089 ± 0.053
2.936ThrThr: 2.936 ± 0.048
4.302ThrVal: 4.302 ± 0.056
0.556ThrTrp: 0.556 ± 0.019
1.845ThrTyr: 1.845 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
4.711ValAla: 4.711 ± 0.06
0.607ValCys: 0.607 ± 0.02
3.436ValAsp: 3.436 ± 0.047
4.593ValGlu: 4.593 ± 0.06
3.33ValPhe: 3.33 ± 0.053
4.649ValGly: 4.649 ± 0.065
1.291ValHis: 1.291 ± 0.027
5.86ValIle: 5.86 ± 0.063
5.036ValLys: 5.036 ± 0.066
6.754ValLeu: 6.754 ± 0.065
1.856ValMet: 1.856 ± 0.038
3.212ValAsn: 3.212 ± 0.05
2.885ValPro: 2.885 ± 0.038
2.271ValGln: 2.271 ± 0.037
2.556ValArg: 2.556 ± 0.047
4.463ValSer: 4.463 ± 0.055
4.096ValThr: 4.096 ± 0.06
5.023ValVal: 5.023 ± 0.073
0.697ValTrp: 0.697 ± 0.021
2.325ValTyr: 2.325 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.7TrpAla: 0.7 ± 0.022
0.097TrpCys: 0.097 ± 0.007
0.574TrpAsp: 0.574 ± 0.02
0.676TrpGlu: 0.676 ± 0.024
0.541TrpPhe: 0.541 ± 0.02
0.75TrpGly: 0.75 ± 0.023
0.217TrpHis: 0.217 ± 0.011
0.813TrpIle: 0.813 ± 0.022
0.795TrpLys: 0.795 ± 0.023
1.168TrpLeu: 1.168 ± 0.032
0.344TrpMet: 0.344 ± 0.013
0.583TrpAsn: 0.583 ± 0.02
0.28TrpPro: 0.28 ± 0.012
0.388TrpGln: 0.388 ± 0.016
0.432TrpArg: 0.432 ± 0.017
0.625TrpSer: 0.625 ± 0.022
0.537TrpThr: 0.537 ± 0.019
0.692TrpVal: 0.692 ± 0.023
0.139TrpTrp: 0.139 ± 0.01
0.385TrpTyr: 0.385 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.06TyrAla: 2.06 ± 0.038
0.315TyrCys: 0.315 ± 0.014
1.859TyrAsp: 1.859 ± 0.04
2.368TyrGlu: 2.368 ± 0.043
1.91TyrPhe: 1.91 ± 0.042
2.589TyrGly: 2.589 ± 0.041
0.839TyrHis: 0.839 ± 0.022
2.476TyrIle: 2.476 ± 0.041
2.132TyrLys: 2.132 ± 0.039
3.544TyrLeu: 3.544 ± 0.053
0.839TyrMet: 0.839 ± 0.023
1.495TyrAsn: 1.495 ± 0.033
1.448TyrPro: 1.448 ± 0.035
1.602TyrGln: 1.602 ± 0.038
1.612TyrArg: 1.612 ± 0.033
2.16TyrSer: 2.16 ± 0.036
1.788TyrThr: 1.788 ± 0.033
2.152TyrVal: 2.152 ± 0.032
0.435TyrTrp: 0.435 ± 0.019
1.487TyrTyr: 1.487 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5368 proteins (1607557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski