Candidatus Electrothrix marina
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2161 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A444JDS5|A0A444JDS5_9DELT CopG antitoxin of type II toxin-antitoxin system OS=Candidatus Electrothrix marina OX=1859130 GN=VU01_11782 PE=4 SV=1
MM1 pKa = 8.22 RR2 pKa = 11.84 KK3 pKa = 9.44 RR4 pKa = 11.84 LWLYY8 pKa = 10.31 LVVAEE13 pKa = 4.95 ICFCFSGVARR23 pKa = 11.84 ADD25 pKa = 3.21 IDD27 pKa = 3.66 PATVQKK33 pKa = 10.78 LFSEE37 pKa = 4.69 DD38 pKa = 5.1 AGATWLFGYY47 pKa = 9.99 SVAVSGNTAVVGSPDD62 pKa = 3.55 FSNGGAAYY70 pKa = 10.38 VFTRR74 pKa = 11.84 SCPEE78 pKa = 3.74 STDD81 pKa = 2.74 WTVQQKK87 pKa = 10.54 LNPPDD92 pKa = 4.23 LGFNLNNSGLDD103 pKa = 3.59 DD104 pKa = 4.63 FGLSVAIDD112 pKa = 3.75 KK113 pKa = 8.87 DD114 pKa = 3.88 TILIGSRR121 pKa = 11.84 SWDD124 pKa = 3.2 SSLNPGGITVTYY136 pKa = 10.3 VFTRR140 pKa = 11.84 SEE142 pKa = 4.49 GVWGFQQEE150 pKa = 4.05 LSIYY154 pKa = 8.03 PTVIDD159 pKa = 4.55 GKK161 pKa = 8.24 WHH163 pKa = 5.35 GVKK166 pKa = 10.33 NVAVHH171 pKa = 6.34 GDD173 pKa = 3.13 IAVVVVGGLRR183 pKa = 11.84 AVLDD187 pKa = 3.76 YY188 pKa = 10.8 DD189 pKa = 3.61 YY190 pKa = 11.31 GIAYY194 pKa = 9.23 VFVRR198 pKa = 11.84 SAGVWRR204 pKa = 11.84 LQQEE208 pKa = 4.02 IAVAGSEE215 pKa = 4.23 EE216 pKa = 4.53 SFGSSVSIDD225 pKa = 3.15 GEE227 pKa = 4.63 TIVFGAMLDD236 pKa = 3.72 DD237 pKa = 5.64 DD238 pKa = 4.83 NGEE241 pKa = 4.21 KK242 pKa = 10.38 SGAAYY247 pKa = 10.43 VYY249 pKa = 10.19 TRR251 pKa = 11.84 TGEE254 pKa = 3.67 IWSQQQKK261 pKa = 9.78 ILCPDD266 pKa = 3.36 AGGGNLFGSSVSLSADD282 pKa = 3.26 TLLIGASSYY291 pKa = 11.46 NEE293 pKa = 3.97 NSSGTAYY300 pKa = 10.06 IFTRR304 pKa = 11.84 FDD306 pKa = 3.88 NEE308 pKa = 3.29 WSLQQSIAYY317 pKa = 6.85 PQLSDD322 pKa = 3.18 PTYY325 pKa = 9.66 YY326 pKa = 11.33 GLFGEE331 pKa = 4.99 GEE333 pKa = 4.55 VVSLDD338 pKa = 3.36 KK339 pKa = 10.71 DD340 pKa = 3.7 TAVIGGGSSAYY351 pKa = 10.42 VFTRR355 pKa = 11.84 SGDD358 pKa = 2.9 VWTQKK363 pKa = 10.25 EE364 pKa = 4.25 QLVPDD369 pKa = 4.02 YY370 pKa = 11.34 GEE372 pKa = 3.72 QGNFGFSVSVDD383 pKa = 2.93 GGNIVIGSEE392 pKa = 4.06 GSVYY396 pKa = 10.36 FYY398 pKa = 11.36 GQGNQCDD405 pKa = 3.84 SDD407 pKa = 4.08 GDD409 pKa = 4.9 SITDD413 pKa = 3.76 DD414 pKa = 3.53 QDD416 pKa = 3.53 NCPLEE421 pKa = 4.3 VNPDD425 pKa = 3.67 QIDD428 pKa = 3.55 TDD430 pKa = 3.76 GDD432 pKa = 4.23 NIGDD436 pKa = 3.9 ACDD439 pKa = 3.9 PRR441 pKa = 11.84 PEE443 pKa = 4.29 EE444 pKa = 4.12 QDD446 pKa = 3.0 SDD448 pKa = 3.54 TDD450 pKa = 3.58 ADD452 pKa = 3.78 EE453 pKa = 4.76 VYY455 pKa = 10.59 DD456 pKa = 4.63 YY457 pKa = 11.28 IDD459 pKa = 3.75 NCSSISNQDD468 pKa = 3.19 QKK470 pKa = 11.41 DD471 pKa = 3.43 TDD473 pKa = 3.79 KK474 pKa = 11.74 DD475 pKa = 3.87 GLGDD479 pKa = 3.71 VCDD482 pKa = 4.63 SDD484 pKa = 3.92 IDD486 pKa = 4.1 SDD488 pKa = 4.88 GVDD491 pKa = 3.71 NEE493 pKa = 4.34 QDD495 pKa = 3.58 NCPLSANPNQRR506 pKa = 11.84 DD507 pKa = 3.55 RR508 pKa = 11.84 DD509 pKa = 3.74 DD510 pKa = 5.49 DD511 pKa = 5.05 GIGNVCDD518 pKa = 3.9 SDD520 pKa = 4.61 VVCSFNPTEE529 pKa = 3.88 AA530 pKa = 3.86
Molecular weight: 56.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A444JEI0|A0A444JEI0_9DELT ATP-dependent DNA helicase DinG OS=Candidatus Electrothrix marina OX=1859130 GN=VU01_11381 PE=4 SV=1
MM1 pKa = 7.48 ARR3 pKa = 11.84 LSGVDD8 pKa = 3.52 LPRR11 pKa = 11.84 NKK13 pKa = 10.46 HH14 pKa = 4.79 MDD16 pKa = 3.27 RR17 pKa = 11.84 ALTYY21 pKa = 9.8 IYY23 pKa = 10.65 GIGLTTARR31 pKa = 11.84 AILDD35 pKa = 3.92 KK36 pKa = 11.31 ADD38 pKa = 5.05 LPYY41 pKa = 11.29 QMNSDD46 pKa = 4.5 DD47 pKa = 5.27 LSGDD51 pKa = 3.4 DD52 pKa = 3.39 VSRR55 pKa = 11.84 IRR57 pKa = 11.84 KK58 pKa = 9.27 IIEE61 pKa = 3.37 SDD63 pKa = 3.96 YY64 pKa = 11.05 VVEE67 pKa = 4.06 GDD69 pKa = 3.41 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 EE73 pKa = 3.53 VAMDD77 pKa = 3.66 IKK79 pKa = 10.84 RR80 pKa = 11.84 HH81 pKa = 5.85 IDD83 pKa = 2.81 LGTYY87 pKa = 9.33 RR88 pKa = 11.84 GRR90 pKa = 11.84 RR91 pKa = 11.84 HH92 pKa = 6.51 RR93 pKa = 11.84 MSLPCRR99 pKa = 11.84 GQRR102 pKa = 11.84 TKK104 pKa = 10.95 TNARR108 pKa = 11.84 TRR110 pKa = 11.84 KK111 pKa = 8.64 GPKK114 pKa = 9.03 RR115 pKa = 11.84 GAAVRR120 pKa = 11.84 KK121 pKa = 9.37 KK122 pKa = 10.59
Molecular weight: 14.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.75
IPC_protein 10.672
Toseland 10.789
ProMoST 10.584
Dawson 10.877
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 11.038
Grimsley 10.921
Solomon 11.023
Lehninger 10.979
Nozaki 10.76
DTASelect 10.613
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.804
Patrickios 10.76
IPC_peptide 11.023
IPC2_peptide 9.56
IPC2.peptide.svr19 8.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2161
0
2161
594014
39
2159
274.9
30.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.297 ± 0.061
1.338 ± 0.022
5.56 ± 0.04
7.014 ± 0.056
4.263 ± 0.04
7.268 ± 0.052
2.089 ± 0.025
6.23 ± 0.044
5.564 ± 0.053
10.455 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.484 ± 0.031
3.589 ± 0.039
4.29 ± 0.031
4.083 ± 0.039
5.676 ± 0.052
5.92 ± 0.044
5.204 ± 0.045
6.668 ± 0.043
1.067 ± 0.02
2.941 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here