Capybara microvirus Cap3_SP_588
Average proteome isoelectric point is 5.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVV0|A0A4V1FVV0_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_588 OX=2585480 PE=4 SV=1
MM1 pKa = 8.34 VEE3 pKa = 4.47 MYY5 pKa = 10.57 AIYY8 pKa = 10.55 DD9 pKa = 3.78 PVSEE13 pKa = 4.36 SVKK16 pKa = 9.96 TPFFIEE22 pKa = 4.55 NYY24 pKa = 8.83 ASCIRR29 pKa = 11.84 AIADD33 pKa = 3.7 AYY35 pKa = 10.05 NHH37 pKa = 6.33 NLFADD42 pKa = 4.47 GAKK45 pKa = 10.37 DD46 pKa = 4.23 VEE48 pKa = 4.35 LLSYY52 pKa = 11.08 GLIQNDD58 pKa = 3.66 GNFYY62 pKa = 10.54 PVDD65 pKa = 3.41 TDD67 pKa = 3.17 IYY69 pKa = 9.27 EE70 pKa = 4.54 SNGVLVPKK78 pKa = 10.76 NFGKK82 pKa = 10.49 VFEE85 pKa = 5.09 LIKK88 pKa = 11.05 DD89 pKa = 3.38 EE90 pKa = 5.09 SEE92 pKa = 3.98 RR93 pKa = 11.84 LKK95 pKa = 11.32 NGG97 pKa = 3.09
Molecular weight: 10.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.209
IPC2_protein 4.38
IPC_protein 4.266
Toseland 4.088
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.126
Rodwell 4.101
Grimsley 3.999
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.507
Thurlkill 4.113
EMBOSS 4.139
Sillero 4.38
Patrickios 2.994
IPC_peptide 4.215
IPC2_peptide 4.355
IPC2.peptide.svr19 4.303
Protein with the highest isoelectric point:
>tr|A0A4V1FVV0|A0A4V1FVV0_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_588 OX=2585480 PE=4 SV=1
MM1 pKa = 7.67 CLNPKK6 pKa = 9.57 KK7 pKa = 10.49 AYY9 pKa = 10.23 LMNYY13 pKa = 9.57 KK14 pKa = 9.94 KK15 pKa = 10.7 DD16 pKa = 3.53 NNKK19 pKa = 9.89 NVILFNKK26 pKa = 9.86 PIYY29 pKa = 9.79 QDD31 pKa = 2.51 WHH33 pKa = 5.56 KK34 pKa = 10.3 IKK36 pKa = 10.73 RR37 pKa = 11.84 EE38 pKa = 3.6 IYY40 pKa = 9.51 IPCGICKK47 pKa = 8.91 EE48 pKa = 4.47 CKK50 pKa = 9.18 MSHH53 pKa = 5.88 AFDD56 pKa = 3.16 WVVRR60 pKa = 11.84 CTAEE64 pKa = 4.05 SLCYY68 pKa = 9.46 EE69 pKa = 3.92 NNYY72 pKa = 9.76 FVTLTYY78 pKa = 11.19 DD79 pKa = 3.23 NDD81 pKa = 4.74 KK82 pKa = 11.24 IPMTPYY88 pKa = 10.57 FGDD91 pKa = 4.37 DD92 pKa = 4.14 DD93 pKa = 4.99 SCFMIPTLRR102 pKa = 11.84 YY103 pKa = 9.75 KK104 pKa = 10.56 DD105 pKa = 3.44 VQDD108 pKa = 3.47 FFKK111 pKa = 10.82 RR112 pKa = 11.84 LRR114 pKa = 11.84 SKK116 pKa = 11.13 LDD118 pKa = 3.33 YY119 pKa = 10.86 NDD121 pKa = 3.76 NGNVRR126 pKa = 11.84 HH127 pKa = 5.74 FTCSEE132 pKa = 3.95 YY133 pKa = 11.23 GGITQRR139 pKa = 11.84 PHH141 pKa = 4.53 YY142 pKa = 10.12 HH143 pKa = 6.56 SILFGINLTDD153 pKa = 3.8 LKK155 pKa = 10.91 YY156 pKa = 10.85 YY157 pKa = 10.8 KK158 pKa = 9.38 KK159 pKa = 9.24 TEE161 pKa = 4.19 NGDD164 pKa = 3.19 ILYY167 pKa = 9.42 TSKK170 pKa = 10.98 LLSDD174 pKa = 4.12 CWQNGFVIVGEE185 pKa = 4.11 VTPEE189 pKa = 3.86 SIAYY193 pKa = 8.53 VSRR196 pKa = 11.84 YY197 pKa = 7.77 SLKK200 pKa = 10.11 KK201 pKa = 10.34 QVDD204 pKa = 3.81 EE205 pKa = 4.95 TYY207 pKa = 11.12 SSYY210 pKa = 11.06 HH211 pKa = 6.24 LSDD214 pKa = 3.49 FRR216 pKa = 11.84 EE217 pKa = 4.22 SPKK220 pKa = 10.71 LYY222 pKa = 8.77 MSRR225 pKa = 11.84 RR226 pKa = 11.84 PGIGAPYY233 pKa = 8.92 YY234 pKa = 9.77 EE235 pKa = 4.5 KK236 pKa = 10.81 HH237 pKa = 6.04 QIHH240 pKa = 6.42 NKK242 pKa = 5.85 WQKK245 pKa = 11.03 LYY247 pKa = 11.08 LNSDD251 pKa = 4.2 LKK253 pKa = 11.39 DD254 pKa = 3.06 IDD256 pKa = 3.72 YY257 pKa = 9.45 PKK259 pKa = 10.81 YY260 pKa = 9.43 FLSMLEE266 pKa = 4.07 INDD269 pKa = 3.42 EE270 pKa = 4.13 EE271 pKa = 5.68 LYY273 pKa = 10.32 IDD275 pKa = 4.38 YY276 pKa = 10.04 MNNKK280 pKa = 9.99 DD281 pKa = 4.45 KK282 pKa = 11.21 INEE285 pKa = 3.99 IKK287 pKa = 10.88 ANAYY291 pKa = 10.08 YY292 pKa = 9.8 PDD294 pKa = 4.48 EE295 pKa = 4.26 KK296 pKa = 10.45 TEE298 pKa = 4.06 SYY300 pKa = 10.47 RR301 pKa = 11.84 LEE303 pKa = 4.1 VQARR307 pKa = 11.84 KK308 pKa = 10.14 YY309 pKa = 10.89 KK310 pKa = 10.39 FDD312 pKa = 3.47 NGYY315 pKa = 10.58 LSIRR319 pKa = 11.84 NEE321 pKa = 3.97 VV322 pKa = 2.98
Molecular weight: 38.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.885
IPC2_protein 7.073
IPC_protein 7.029
Toseland 6.795
ProMoST 7.541
Dawson 7.688
Bjellqvist 7.922
Wikipedia 7.614
Rodwell 7.702
Grimsley 6.854
Solomon 7.746
Lehninger 7.761
Nozaki 8.053
DTASelect 7.819
Thurlkill 7.863
EMBOSS 7.922
Sillero 8.141
Patrickios 3.389
IPC_peptide 7.732
IPC2_peptide 7.161
IPC2.peptide.svr19 7.275
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1369
97
579
273.8
31.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.501 ± 1.557
0.95 ± 0.538
6.793 ± 0.465
5.186 ± 0.638
5.186 ± 0.522
6.355 ± 0.773
1.899 ± 0.288
6.209 ± 0.325
5.698 ± 1.691
6.501 ± 0.43
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.899 ± 0.235
7.889 ± 0.724
4.602 ± 0.825
3.725 ± 0.808
4.018 ± 0.593
8.4 ± 0.778
4.894 ± 0.572
5.478 ± 0.801
1.242 ± 0.328
6.574 ± 0.975
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here