Camel associated porprismacovirus 1
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1ENV3|A0A0A1ENV3_9VIRU Uncharacterized protein OS=Camel associated porprismacovirus 1 OX=2170105 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.01 SQSITVSIVEE11 pKa = 4.58 TYY13 pKa = 10.93 DD14 pKa = 3.91 LSTTEE19 pKa = 4.0 NRR21 pKa = 11.84 MGLIAIKK28 pKa = 8.7 TPSMTAVNKK37 pKa = 10.05 RR38 pKa = 11.84 YY39 pKa = 9.51 PGFIRR44 pKa = 11.84 NFKK47 pKa = 9.35 FLHH50 pKa = 5.86 VNSCDD55 pKa = 3.62 VVLSCASTLPTDD67 pKa = 4.08 PLQIGTTAGKK77 pKa = 9.78 VAPQDD82 pKa = 3.32 MFNPLLYY89 pKa = 10.4 KK90 pKa = 10.52 AVSNDD95 pKa = 2.65 SWNGLLSRR103 pKa = 11.84 IYY105 pKa = 10.41 SPTGASVSLNDD116 pKa = 4.69 DD117 pKa = 3.18 VRR119 pKa = 11.84 HH120 pKa = 5.79 FADD123 pKa = 4.55 AFPDD127 pKa = 3.57 ATSTEE132 pKa = 4.45 SVRR135 pKa = 11.84 AYY137 pKa = 10.75 YY138 pKa = 11.29 AMLADD143 pKa = 4.05 PSFKK147 pKa = 10.1 KK148 pKa = 10.69 AHH150 pKa = 5.72 IQAGLSMRR158 pKa = 11.84 GLRR161 pKa = 11.84 PLVYY165 pKa = 10.02 HH166 pKa = 6.84 LLSGSGTNTEE176 pKa = 4.18 LTGAGQPTAVGQAYY190 pKa = 9.69 KK191 pKa = 10.88 NYY193 pKa = 10.08 VDD195 pKa = 5.29 DD196 pKa = 6.0 SGDD199 pKa = 3.31 AYY201 pKa = 10.95 QRR203 pKa = 11.84 ASNNLYY209 pKa = 10.78 SGTTLFKK216 pKa = 10.71 GRR218 pKa = 11.84 PVPMPSVQTTPAVVHH233 pKa = 6.49 PSDD236 pKa = 3.71 GTVTQIDD243 pKa = 4.41 YY244 pKa = 11.15 NVVLGGIPSTYY255 pKa = 9.05 VCAIVVPPHH264 pKa = 6.31 SLNTTYY270 pKa = 10.78 FRR272 pKa = 11.84 MTVKK276 pKa = 10.31 WSVTFHH282 pKa = 5.79 TVASEE287 pKa = 3.84 MSKK290 pKa = 11.28 ALMPGMSDD298 pKa = 3.5 LGRR301 pKa = 11.84 YY302 pKa = 8.77 AYY304 pKa = 7.75 YY305 pKa = 10.33 TSEE308 pKa = 4.13 TSKK311 pKa = 11.1 LEE313 pKa = 3.85 PVPIEE318 pKa = 4.5 DD319 pKa = 3.99 VSDD322 pKa = 3.56 TAVTGDD328 pKa = 3.48 TVDD331 pKa = 3.54 ADD333 pKa = 4.26 GVSLDD338 pKa = 4.39 LVMEE342 pKa = 4.57 KK343 pKa = 10.8
Molecular weight: 37.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.344
IPC2_protein 5.385
IPC_protein 5.385
Toseland 5.703
ProMoST 5.69
Dawson 5.575
Bjellqvist 5.614
Wikipedia 5.563
Rodwell 5.525
Grimsley 5.83
Solomon 5.575
Lehninger 5.55
Nozaki 5.804
DTASelect 6.008
Thurlkill 5.906
EMBOSS 5.881
Sillero 5.881
Patrickios 3.567
IPC_peptide 5.588
IPC2_peptide 5.893
IPC2.peptide.svr19 5.876
Protein with the highest isoelectric point:
>tr|A0A0A1EJ70|A0A0A1EJ70_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 1 OX=2170105 GN=rep PE=4 SV=1
MM1 pKa = 7.27 YY2 pKa = 9.05 PILPLLVILSMPMVFLSILSWRR24 pKa = 11.84 NDD26 pKa = 3.08 MASYY30 pKa = 9.97 RR31 pKa = 11.84 QYY33 pKa = 11.09 RR34 pKa = 11.84 HH35 pKa = 5.42 QSSSNFGRR43 pKa = 11.84 PSSMRR48 pKa = 11.84 FDD50 pKa = 4.48 LDD52 pKa = 2.9 PRR54 pKa = 11.84 TRR56 pKa = 11.84 YY57 pKa = 9.38 EE58 pKa = 3.79 LSKK61 pKa = 11.27 VPFIGDD67 pKa = 3.63 AFRR70 pKa = 11.84 YY71 pKa = 8.48 MDD73 pKa = 3.13 QTKK76 pKa = 10.65 YY77 pKa = 10.85 YY78 pKa = 10.29 NDD80 pKa = 3.51 YY81 pKa = 10.21 LASRR85 pKa = 11.84 GMTWNDD91 pKa = 2.63 VKK93 pKa = 11.36 YY94 pKa = 9.47 PALLSGSGAYY104 pKa = 9.31 GVAASGYY111 pKa = 8.17 RR112 pKa = 11.84 VSKK115 pKa = 11.04 NFLKK119 pKa = 10.81 LYY121 pKa = 10.13 RR122 pKa = 4.74
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.62
IPC2_protein 9.78
IPC_protein 10.189
Toseland 9.663
ProMoST 9.78
Dawson 10.043
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.116
Grimsley 10.189
Solomon 10.072
Lehninger 10.014
Nozaki 9.633
DTASelect 9.838
Thurlkill 9.823
EMBOSS 10.116
Sillero 9.94
Patrickios 4.291
IPC_peptide 10.058
IPC2_peptide 8.712
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
732
122
343
244.0
27.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.557 ± 0.808
1.23 ± 0.47
6.148 ± 0.163
4.372 ± 1.766
3.552 ± 0.417
6.694 ± 0.242
2.049 ± 0.318
4.781 ± 1.082
5.601 ± 1.42
7.923 ± 0.687
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.279 ± 0.708
3.689 ± 0.323
4.918 ± 1.107
2.732 ± 0.121
6.284 ± 1.678
8.743 ± 1.599
7.65 ± 1.518
7.104 ± 1.522
1.776 ± 0.812
4.918 ± 1.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here