Solitalea longa
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3780 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S4ZWT7|A0A2S4ZWT7_9SPHI Short-chain dehydrogenase OS=Solitalea longa OX=2079460 GN=C3K47_18705 PE=3 SV=1
YYY2 pKa = 9.99 GSDDD6 pKa = 3.06 FEEE9 pKa = 4.1 QVSDDD14 pKa = 3.42 HHH16 pKa = 6.47 GTVSQLINITISPVNDDD33 pKa = 3.17 PAAADDD39 pKa = 3.22 HH40 pKa = 6.85 YYY42 pKa = 11.16 MDDD45 pKa = 4.15 EE46 pKa = 4.99 DD47 pKa = 5.96 DD48 pKa = 4.1 TQTGNVLTDDD58 pKa = 3.78 PAASDDD64 pKa = 3.1 DDD66 pKa = 4.39 DDD68 pKa = 3.96 LTAVLINDDD77 pKa = 4.93 PPEEE81 pKa = 4.64 GTLLLNADDD90 pKa = 4.2 SFSFIPAADDD100 pKa = 3.96 SGTVSFSYYY109 pKa = 9.66 ACDDD113 pKa = 2.92 GQPQLCSEEE122 pKa = 4.35 EE123 pKa = 4.02 SVTITVSPVNDDD135 pKa = 3.53 PVATSPVQYYY145 pKa = 9.79 EE146 pKa = 4.25 NEEE149 pKa = 4.15 DD150 pKa = 3.97 PVEEE154 pKa = 4.21 DD155 pKa = 4.34 LVASDDD161 pKa = 4.19 DDD163 pKa = 3.89 DDD165 pKa = 3.98 LTFSKKK171 pKa = 10.93 SDDD174 pKa = 3.71 ANGTVTVAADDD185 pKa = 3.66 SFTYYY190 pKa = 10.68 PAANYYY196 pKa = 10.3 HH197 pKa = 6.23 DDD199 pKa = 3.42 DD200 pKa = 3.56 FTARR204 pKa = 11.84 VSDDD208 pKa = 3.93 NGGSVVISVNITVMPVNDDD227 pKa = 4.22 PTAVSPISIATDDD240 pKa = 3.2 EE241 pKa = 4.15 VVFNGQLTADDD252 pKa = 4.16 DD253 pKa = 4.54 DDD255 pKa = 4.31 EEE257 pKa = 4.68 LTFTLEEE264 pKa = 4.03 QSAHHH269 pKa = 4.75 TVVLGNTSHHH279 pKa = 7.02 GTTSTLEEE287 pKa = 3.9 YY288 pKa = 9.38 YYY290 pKa = 10.69 PAGGYYY296 pKa = 10.17 KKK298 pKa = 9.76 DD299 pKa = 3.48 DD300 pKa = 3.77 HH301 pKa = 6.55 QVQIKKK307 pKa = 9.72 DD308 pKa = 3.36 AGATTLIHHH317 pKa = 5.46 HHH319 pKa = 5.47 LVNPVNDDD327 pKa = 3.77 PVIEEE332 pKa = 4.35 AGPFTIDDD340 pKa = 3.72 EE341 pKa = 4.38 DD342 pKa = 4.46 EEE344 pKa = 4.18 TGVFKKK350 pKa = 10.6 TDDD353 pKa = 3.18 DDD355 pKa = 4.22 DDD357 pKa = 4.2 LTFSVQTAPGTGTLTLQADDD377 pKa = 4.39 SFSYYY382 pKa = 10.91 PLANYYY388 pKa = 9.68 GSDDD392 pKa = 3.21 FEEE395 pKa = 4.1 QVSDDD400 pKa = 3.42 HHH402 pKa = 6.47 GTVSQLINITISPVNDDD419 pKa = 3.17 PAAADDD425 pKa = 3.22 HH426 pKa = 6.85 YYY428 pKa = 11.17 MDDD431 pKa = 3.76 EE432 pKa = 4.98 DD433 pKa = 4.97 GTQTGNVLTDDD444 pKa = 3.78 PAASDDD450 pKa = 3.1 DDD452 pKa = 4.39 DDD454 pKa = 3.96 LTAVLINDDD463 pKa = 4.74 PPEEE467 pKa = 4.42 EE468 pKa = 4.52 TLLLNADDD476 pKa = 4.1 SFSFTPAADDD486 pKa = 4.14 SGTASFSYYY495 pKa = 9.5 ACDDD499 pKa = 2.94 GLPQLCSEEE508 pKa = 4.43 EE509 pKa = 4.06 SVTITVNSVNDDD521 pKa = 3.67 PVADDD526 pKa = 4.68 SIQYYY531 pKa = 10.03 EE532 pKa = 4.48 TEEE535 pKa = 4.05 DD536 pKa = 3.68 PVEEE540 pKa = 4.07 DD541 pKa = 3.98 LIVTDDD547 pKa = 5.27 DD548 pKa = 5.12 DD549 pKa = 4.87 DDD551 pKa = 3.55 LTFVLKKK558 pKa = 10.55 GAEEE562 pKa = 4.05 QHHH565 pKa = 6.56 IVSLTIDDD573 pKa = 3.37 AFVYYY578 pKa = 10.73 PTANYYY584 pKa = 10.59 GTDDD588 pKa = 3.28 FTVIVSDDD596 pKa = 3.93 QGGNIEEE603 pKa = 4.52 IINIQIDDD611 pKa = 4.07 VNDDD615 pKa = 3.45 PVAVSPIALTTDDD628 pKa = 2.93 EE629 pKa = 5.28 DD630 pKa = 4.4 PVSGQITVSDDD641 pKa = 3.63 DDD643 pKa = 4.18 DDD645 pKa = 4.04 LSYYY649 pKa = 10.77 SSEEE653 pKa = 4.26 VNGSVIVNPDDD664 pKa = 2.59 SFTYYY669 pKa = 9.84 PKKK672 pKa = 10.19 DDD674 pKa = 3.74 YY675 pKa = 9.83 GKKK678 pKa = 10.28 DD679 pKa = 3.24 LVVTITDDD687 pKa = 3.76 NGGNAVVKKK696 pKa = 10.0 YYY698 pKa = 10.86 EEE700 pKa = 4.09 NPVNDDD706 pKa = 3.84 PVAITPIEEE715 pKa = 4.03 QTNKKK720 pKa = 10.51 IAVGSNLSATDDD732 pKa = 3.94 EEE734 pKa = 5.12 EE735 pKa = 4.2 DD736 pKa = 3.14 LMYYY740 pKa = 10.46 VAANPQQGSLIVNADDD756 pKa = 2.87 SFIYYY761 pKa = 9.96 PNAGFVGVDDD771 pKa = 3.22 HH772 pKa = 7.01 SAFVDDD778 pKa = 3.99 DD779 pKa = 5.01 NGGTASIQVNINVLPINNSPRR800 pKa = 11.84 ATLIQAFTVNEEE812 pKa = 4.23 DD813 pKa = 3.47 EE814 pKa = 4.53 LSATVEEE821 pKa = 3.99 SDDD824 pKa = 4.72 DDD826 pKa = 3.84 DDD828 pKa = 3.98 LNFVILTPASNGQLQLNQQTGAFIYYY854 pKa = 9.91 PNKKK858 pKa = 10.04 DD859 pKa = 3.7 YY860 pKa = 11.62 GTDDD864 pKa = 3.18 FSVNIDDD871 pKa = 3.74 DD872 pKa = 4.61 NEEE875 pKa = 3.69 LIQVLVNISIIPVNDDD891 pKa = 2.98 PIAVTPVSEEE901 pKa = 4.16 LEEE904 pKa = 4.26 EE905 pKa = 4.73 DD906 pKa = 3.63 SLSSSVSATDDD917 pKa = 4.46 DDD919 pKa = 4.9 DD920 pKa = 5.76 DD921 pKa = 4.62 LTYYY925 pKa = 10.56 INTVPLHHH933 pKa = 6.53 DDD935 pKa = 3.03 LINDDD940 pKa = 4.53 DD941 pKa = 3.16 TYYY944 pKa = 9.37 YYY946 pKa = 11.0 PFADDD951 pKa = 4.55 NGTDDD956 pKa = 3.54 FIVTVDDD963 pKa = 3.7 DD964 pKa = 4.63 NGGNAQITVQLEEE977 pKa = 3.9 TPDDD981 pKa = 3.09 DDD983 pKa = 3.48 PVAQNISLITDDD995 pKa = 3.26 EE996 pKa = 5.95 DD997 pKa = 3.72 QLTGTLTATDDD1008 pKa = 3.39 DDD1010 pKa = 4.22 DDD1012 pKa = 3.98 LAFTVISEEE1021 pKa = 4.2 ANGLFEEE1028 pKa = 5.45 SQNGDDD1034 pKa = 3.38 YY1035 pKa = 10.81 YYY1037 pKa = 10.81 PYYY1040 pKa = 10.95 NFNGADDD1047 pKa = 3.54 VSMQVNDDD1055 pKa = 4.08 NGGLASFKKK1064 pKa = 10.74 YYY1066 pKa = 10.5 EEE1068 pKa = 3.79 RR1069 pKa = 11.84 SVYYY1073 pKa = 9.95 DD1074 pKa = 3.31 PIAFEEE1080 pKa = 4.07 SFVMDDD1086 pKa = 4.18 EE1087 pKa = 4.8 EEE1089 pKa = 4.15 LNNKKK1094 pKa = 9.69 DDD1096 pKa = 4.77 ISVDDD1101 pKa = 3.75 NPLTFEEE1108 pKa = 5.0 ITSTQNGIVQVNADDD1123 pKa = 3.47 SFTYYY1128 pKa = 10.12 PNPNFNGSDDD1138 pKa = 3.46 FKKK1141 pKa = 11.06 RR1142 pKa = 11.84 VKKK1145 pKa = 10.69 DD1146 pKa = 3.87 DD1147 pKa = 3.49 KK1148 pKa = 11.61 GSDDD1152 pKa = 3.92 EE1153 pKa = 4.28 TVSILINPVNSPPEEE1168 pKa = 3.72 ATPINLTINEEE1179 pKa = 4.16 DD1180 pKa = 3.2 PYYY1183 pKa = 10.36 GKKK1186 pKa = 10.4 IAVDDD1191 pKa = 3.69 EEE1193 pKa = 4.22 DDD1195 pKa = 4.06 ITFVIGAVVPEEE1207 pKa = 4.68 HH1208 pKa = 6.1 TLTVNSDDD1216 pKa = 2.73 SYYY1219 pKa = 10.75 YYY1221 pKa = 10.92 PAKKK1225 pKa = 10.41 DD1226 pKa = 3.81 YY1227 pKa = 10.87 GSDDD1231 pKa = 3.14 FTARR1235 pKa = 11.84 VQDDD1239 pKa = 4.02 DD1240 pKa = 3.61 KK1241 pKa = 12.0 GLSTIAVNITIVPVNDDD1258 pKa = 3.89 PVSSNLAYYY1267 pKa = 10.3 DD1268 pKa = 3.52 LFNEEE1273 pKa = 4.56 RR1274 pKa = 11.84 TLNISEEE1281 pKa = 4.33 VSDDD1285 pKa = 4.49 DDD1287 pKa = 3.89 PLPLVYYY1294 pKa = 10.22 VSTAPQHHH1302 pKa = 5.33 TITQMEEE1309 pKa = 4.64 GNPVYYY1315 pKa = 10.47 PDDD1318 pKa = 4.04 NYYY1321 pKa = 10.82 GPDDD1325 pKa = 3.13 FAYYY1329 pKa = 7.31 VCDDD1333 pKa = 3.53 FSPEEE1338 pKa = 3.71 CTTSTVEEE1346 pKa = 3.7 TVKKK1350 pKa = 10.56 KK1351 pKa = 10.67 LVDDD1355 pKa = 3.93 QITKKK1360 pKa = 9.01 ASITKKK1366 pKa = 9.8 DDD1368 pKa = 4.24 NQALSFNLLVKKK1380 pKa = 11.03 SDDD1383 pKa = 3.42 VNAATQVTVIDDD1395 pKa = 3.97 LPEEE1399 pKa = 4.02 FSYYY1403 pKa = 11.18 SANATLGNVTITGSNIVTWEEE1424 pKa = 3.83 SSLAAQQSAQLTIDDD1439 pKa = 3.3 TAVQGGDDD1447 pKa = 4.15 INSATVTAIEEE1458 pKa = 4.55 EEE1460 pKa = 4.45 NPSDDD1465 pKa = 3.58 KKK1467 pKa = 10.73 SVSVEEE1473 pKa = 3.73 FGFKKK1478 pKa = 9.74 ANVFTPNGDDD1488 pKa = 3.99 INDDD1492 pKa = 3.68 FTINGVEEE1500 pKa = 4.3 DD1501 pKa = 4.33 DDD1503 pKa = 6.37 EEE1505 pKa = 4.42 QVFNRR1510 pKa = 11.84 WGAEEE1515 pKa = 3.7 YYY1517 pKa = 10.17 KK1518 pKa = 10.35 KKK1520 pKa = 10.32 YYY1522 pKa = 10.62 KK1523 pKa = 10.3 DDD1525 pKa = 3.09 NGSDDD1530 pKa = 4.45 PEEE1533 pKa = 3.92 TYYY1536 pKa = 10.25 YYY1538 pKa = 10.76 LRR1540 pKa = 11.84 IKKK1543 pKa = 10.66 KKK1545 pKa = 9.03 GNTKKK1550 pKa = 9.9 YYY1552 pKa = 10.1 GYYY1555 pKa = 8.6 TILRR1559 pKa = 11.84
Molecular weight: 164.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.465
IPC_protein 3.528
Toseland 3.287
ProMoST 3.706
Dawson 3.528
Bjellqvist 3.681
Wikipedia 3.478
Rodwell 3.338
Grimsley 3.185
Solomon 3.528
Lehninger 3.49
Nozaki 3.63
DTASelect 3.923
Thurlkill 3.338
EMBOSS 3.49
Sillero 3.656
Patrickios 0.604
IPC_peptide 3.516
IPC2_peptide 3.63
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|A0A2S4ZZV3|A0A2S4ZZV3_9SPHI Uncharacterized protein OS=Solitalea longa OX=2079460 GN=C3K47_15700 PE=4 SV=1
MM1 pKa = 7.32 NNARR5 pKa = 11.84 KK6 pKa = 10.01 NEE8 pKa = 3.97 IKK10 pKa = 10.86 DD11 pKa = 3.37 NAFKK15 pKa = 11.13 VFALVCTSVGLLVLGLLLYY34 pKa = 10.67 NIISVGLTRR43 pKa = 11.84 INWDD47 pKa = 4.44 FITNLPSRR55 pKa = 11.84 RR56 pKa = 11.84 PGKK59 pKa = 10.43 AGIYY63 pKa = 7.46 TALLGMIWVVTLTLVISFPIGIGAGVYY90 pKa = 10.25 LEE92 pKa = 4.81 EE93 pKa = 4.94 YY94 pKa = 9.08 GAKK97 pKa = 10.02 NRR99 pKa = 11.84 FAKK102 pKa = 10.51 FIEE105 pKa = 4.04 INIANLAGVPSIIYY119 pKa = 9.93 GILGLEE125 pKa = 3.93 LFGRR129 pKa = 11.84 LLSLGNSILAASLTLSLLVLPIVIVATRR157 pKa = 11.84 EE158 pKa = 4.09 AVKK161 pKa = 10.5 SVPNSLRR168 pKa = 11.84 EE169 pKa = 3.88 ASIGLGATRR178 pKa = 11.84 WQTISRR184 pKa = 11.84 IVLPASFGSILTGLILAVSRR204 pKa = 11.84 AVGEE208 pKa = 4.12 TAPLIVVGALVYY220 pKa = 11.0 VPFAPKK226 pKa = 10.48 SPLDD230 pKa = 3.51 QFTVLPIQIFNWTSRR245 pKa = 11.84 PQSGFVVNAAAAIIILLLFVFILNGIAVFLRR276 pKa = 11.84 NRR278 pKa = 11.84 HH279 pKa = 4.37 NRR281 pKa = 11.84 RR282 pKa = 11.84 VKK284 pKa = 10.12 YY285 pKa = 10.45
Molecular weight: 30.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.838
IPC_protein 10.584
Toseland 10.57
ProMoST 10.292
Dawson 10.716
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.979
Grimsley 10.774
Solomon 10.789
Lehninger 10.76
Nozaki 10.54
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.672
IPC_peptide 10.789
IPC2_peptide 9.311
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3780
0
3780
1322964
38
4393
350.0
39.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.086 ± 0.041
0.859 ± 0.015
5.222 ± 0.029
6.015 ± 0.048
5.081 ± 0.03
6.627 ± 0.043
1.726 ± 0.017
7.41 ± 0.037
7.34 ± 0.051
9.418 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.223 ± 0.021
5.953 ± 0.035
3.681 ± 0.026
3.645 ± 0.022
3.527 ± 0.026
6.688 ± 0.037
5.87 ± 0.064
6.462 ± 0.034
1.125 ± 0.015
4.041 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here