Oxalobacteraceae bacterium
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2416 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q3HRJ3|A0A4Q3HRJ3_9BURK Phosphoenolpyruvate synthase (Fragment) OS=Oxalobacteraceae bacterium OX=1871070 GN=EOO79_12565 PE=4 SV=1
MM1 pKa = 7.44 SKK3 pKa = 9.6 WKK5 pKa = 10.43 HH6 pKa = 5.82 FIAAVLLGASTQAWAAPILSFSTGPAAQVGSTVTYY41 pKa = 10.67 NIAIDD46 pKa = 5.16 DD47 pKa = 3.87 IADD50 pKa = 4.16 LYY52 pKa = 11.38 SYY54 pKa = 10.53 QFSINYY60 pKa = 7.5 DD61 pKa = 3.07 ARR63 pKa = 11.84 YY64 pKa = 9.64 LRR66 pKa = 11.84 ALNVTEE72 pKa = 4.38 GAFLGTAGSTVSGVLGMDD90 pKa = 3.26 TGLIDD95 pKa = 3.79 FVYY98 pKa = 10.66 GSLLGPTPGASGSGLLASITFEE120 pKa = 4.77 AIGAGTSALSFADD133 pKa = 4.49 VIFLDD138 pKa = 4.71 SVGDD142 pKa = 4.34 DD143 pKa = 3.38 IAGLTALSGQGVVLADD159 pKa = 3.43 PGGPVDD165 pKa = 3.87 VPEE168 pKa = 4.54 PASVLLL174 pKa = 4.17
Molecular weight: 17.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.706
IPC_protein 3.681
Toseland 3.452
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.694
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.63
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.694
Sillero 3.808
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A4V1TC56|A0A4V1TC56_9BURK Delta-aminolevulinic acid dehydratase (Fragment) OS=Oxalobacteraceae bacterium OX=1871070 GN=hemB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 QVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2416
0
2416
599894
22
1029
248.3
27.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.951 ± 0.067
0.811 ± 0.016
5.869 ± 0.04
5.575 ± 0.049
3.941 ± 0.036
8.332 ± 0.046
2.103 ± 0.026
5.312 ± 0.038
4.457 ± 0.046
9.383 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.028
3.592 ± 0.036
4.605 ± 0.032
3.651 ± 0.031
5.865 ± 0.04
5.42 ± 0.043
5.715 ± 0.041
7.753 ± 0.039
1.187 ± 0.023
2.765 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here