Lapillicoccus jejuensis
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4122 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A542E511|A0A542E511_9MICO Fatty-acyl-CoA synthase OS=Lapillicoccus jejuensis OX=402171 GN=FB458_3484 PE=4 SV=1
MM1 pKa = 7.84 SDD3 pKa = 4.16 LEE5 pKa = 4.36 YY6 pKa = 10.38 PDD8 pKa = 3.7 TLRR11 pKa = 11.84 YY12 pKa = 7.41 TADD15 pKa = 3.58 HH16 pKa = 6.41 EE17 pKa = 4.63 WVQEE21 pKa = 4.16 RR22 pKa = 11.84 EE23 pKa = 4.2 DD24 pKa = 3.38 GTIRR28 pKa = 11.84 IGITAYY34 pKa = 10.49 AQDD37 pKa = 3.56 ALGDD41 pKa = 3.76 VVYY44 pKa = 10.89 VQLPAVGDD52 pKa = 3.93 TVTVGDD58 pKa = 3.59 TCGEE62 pKa = 4.2 VEE64 pKa = 4.37 STKK67 pKa = 10.76 SVSDD71 pKa = 3.33 VYY73 pKa = 11.53 APVTGEE79 pKa = 3.95 VTEE82 pKa = 4.42 VNAALDD88 pKa = 3.85 GTPEE92 pKa = 4.3 LVNSAPYY99 pKa = 9.3 TDD101 pKa = 2.66 GWMYY105 pKa = 11.04 AVRR108 pKa = 11.84 PSGEE112 pKa = 4.16 LPGDD116 pKa = 3.59 LMDD119 pKa = 4.48 AATYY123 pKa = 10.42 RR124 pKa = 11.84 GTLGG128 pKa = 3.35
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A542DWJ2|A0A542DWJ2_9MICO Fructose-1 6-bisphosphatase OS=Lapillicoccus jejuensis OX=402171 GN=FB458_0477 PE=3 SV=1
MM1 pKa = 7.36 GFSFRR6 pKa = 11.84 KK7 pKa = 7.79 TIRR10 pKa = 11.84 VGPLRR15 pKa = 11.84 LTASRR20 pKa = 11.84 RR21 pKa = 11.84 GLGTSVGMGSFRR33 pKa = 11.84 VQSSRR38 pKa = 11.84 GRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 ASARR46 pKa = 11.84 LPFGLNWRR54 pKa = 11.84 GRR56 pKa = 11.84 VV57 pKa = 3.28
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.34
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.251
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4122
0
4122
1392142
30
2163
337.7
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.861 ± 0.058
0.634 ± 0.009
6.383 ± 0.032
4.962 ± 0.04
2.414 ± 0.021
9.754 ± 0.037
2.148 ± 0.019
2.371 ± 0.034
1.526 ± 0.029
10.535 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.512 ± 0.015
1.371 ± 0.018
6.176 ± 0.034
2.747 ± 0.022
8.098 ± 0.052
5.154 ± 0.031
6.606 ± 0.045
10.348 ± 0.044
1.552 ± 0.017
1.846 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here