Vibrio phage vB_ValM_R10Z

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Schizotequatrovirus; unclassified Schizotequatrovirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 385 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LBI0|A0A7G8LBI0_9CAUD Uncharacterized protein OS=Vibrio phage vB_ValM_R10Z OX=2759334 GN=vBValMR10Z_61 PE=4 SV=1
MM1 pKa = 7.83SYY3 pKa = 10.78TDD5 pKa = 3.63ISAFGDD11 pKa = 3.43PVVYY15 pKa = 9.65VHH17 pKa = 7.19AGHH20 pKa = 5.61EE21 pKa = 4.46TILKK25 pKa = 9.97VEE27 pKa = 4.14YY28 pKa = 10.67TEE30 pKa = 4.89DD31 pKa = 3.66CYY33 pKa = 11.8SFFDD37 pKa = 3.7YY38 pKa = 10.38RR39 pKa = 11.84SKK41 pKa = 11.31YY42 pKa = 10.63LYY44 pKa = 9.83TEE46 pKa = 3.68QSIDD50 pKa = 3.83DD51 pKa = 4.35LEE53 pKa = 4.23IHH55 pKa = 6.73LKK57 pKa = 10.65SIDD60 pKa = 3.51VGLDD64 pKa = 3.01DD65 pKa = 6.63DD66 pKa = 5.9LPLNGNIDD74 pKa = 3.82KK75 pKa = 9.27DD76 pKa = 4.12TIPDD80 pKa = 3.92GEE82 pKa = 4.21FLEE85 pKa = 4.65

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LBZ7|A0A7G8LBZ7_9CAUD Baseplate-tail tube initiator OS=Vibrio phage vB_ValM_R10Z OX=2759334 GN=vBValMR10Z_229 PE=4 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84PEE5 pKa = 3.75RR6 pKa = 11.84AKK8 pKa = 10.56KK9 pKa = 10.3KK10 pKa = 10.47KK11 pKa = 10.06EE12 pKa = 3.85SLLDD16 pKa = 3.41SFKK19 pKa = 11.33AQTHH23 pKa = 4.89EE24 pKa = 5.22LGRR27 pKa = 11.84QPIAKK32 pKa = 9.28NQRR35 pKa = 11.84KK36 pKa = 8.85SLAWFKK42 pKa = 11.19KK43 pKa = 9.13RR44 pKa = 11.84VHH46 pKa = 6.28DD47 pKa = 5.28AIRR50 pKa = 11.84SRR52 pKa = 11.84KK53 pKa = 6.71VQRR56 pKa = 11.84PHH58 pKa = 6.7KK59 pKa = 9.88GNMYY63 pKa = 10.03VFAYY67 pKa = 9.78DD68 pKa = 4.2AKK70 pKa = 10.91HH71 pKa = 6.54KK72 pKa = 10.86DD73 pKa = 3.31KK74 pKa = 11.27LPYY77 pKa = 8.59WDD79 pKa = 5.11KK80 pKa = 10.65FPCIICLGVEE90 pKa = 3.89KK91 pKa = 10.72GYY93 pKa = 9.3MLGLNLHH100 pKa = 6.75YY101 pKa = 10.26IPPKK105 pKa = 10.34DD106 pKa = 3.43RR107 pKa = 11.84EE108 pKa = 4.14KK109 pKa = 10.98FLTILLRR116 pKa = 11.84YY117 pKa = 7.42ATTKK121 pKa = 9.54TVSNTTRR128 pKa = 11.84LSVDD132 pKa = 2.59WGRR135 pKa = 11.84VKK137 pKa = 10.66NIKK140 pKa = 9.62FSKK143 pKa = 10.51HH144 pKa = 3.75MVKK147 pKa = 10.48LYY149 pKa = 9.08ILKK152 pKa = 10.17RR153 pKa = 11.84IKK155 pKa = 10.73GSLEE159 pKa = 3.56EE160 pKa = 4.33VKK162 pKa = 10.64PHH164 pKa = 6.31DD165 pKa = 4.54WYY167 pKa = 11.71NVIHH171 pKa = 6.55MPLQQFVAKK180 pKa = 10.06NGRR183 pKa = 11.84NISAVRR189 pKa = 11.84AYY191 pKa = 10.48DD192 pKa = 3.18DD193 pKa = 3.49RR194 pKa = 11.84YY195 pKa = 10.65RR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 3.37

Molecular weight: 23.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

385

0

385

75421

31

1399

195.9

22.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.824 ± 0.124

1.224 ± 0.059

6.954 ± 0.115

7.581 ± 0.15

4.242 ± 0.085

6.106 ± 0.133

2.295 ± 0.063

6.543 ± 0.102

6.628 ± 0.182

7.502 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.08

5.252 ± 0.117

3.047 ± 0.074

3.372 ± 0.082

4.954 ± 0.089

6.326 ± 0.14

6.063 ± 0.141

6.74 ± 0.102

1.374 ± 0.055

4.231 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski