Amino acid dipepetide frequency for Vibrio phage vB_ValM_R10Z

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.264AlaAla: 5.264 ± 0.362
0.796AlaCys: 0.796 ± 0.127
4.322AlaAsp: 4.322 ± 0.267
5.396AlaGlu: 5.396 ± 0.311
2.718AlaPhe: 2.718 ± 0.223
4.23AlaGly: 4.23 ± 0.294
1.339AlaHis: 1.339 ± 0.132
4.773AlaIle: 4.773 ± 0.272
4.389AlaLys: 4.389 ± 0.323
5.37AlaLeu: 5.37 ± 0.264
1.87AlaMet: 1.87 ± 0.181
3.355AlaAsn: 3.355 ± 0.224
1.843AlaPro: 1.843 ± 0.161
2.586AlaGln: 2.586 ± 0.219
3.487AlaArg: 3.487 ± 0.208
4.11AlaSer: 4.11 ± 0.269
4.309AlaThr: 4.309 ± 0.335
4.349AlaVal: 4.349 ± 0.275
0.888AlaTrp: 0.888 ± 0.13
2.851AlaTyr: 2.851 ± 0.199
0.0AlaXaa: 0.0 ± 0.0
Cys
0.743CysAla: 0.743 ± 0.093
0.278CysCys: 0.278 ± 0.111
1.021CysAsp: 1.021 ± 0.12
0.928CysGlu: 0.928 ± 0.115
0.385CysPhe: 0.385 ± 0.068
0.888CysGly: 0.888 ± 0.109
0.385CysHis: 0.385 ± 0.073
0.782CysIle: 0.782 ± 0.108
1.047CysLys: 1.047 ± 0.111
0.756CysLeu: 0.756 ± 0.115
0.239CysMet: 0.239 ± 0.065
0.65CysAsn: 0.65 ± 0.088
0.424CysPro: 0.424 ± 0.074
0.371CysGln: 0.371 ± 0.065
0.597CysArg: 0.597 ± 0.083
0.663CysSer: 0.663 ± 0.123
0.676CysThr: 0.676 ± 0.086
0.716CysVal: 0.716 ± 0.104
0.172CysTrp: 0.172 ± 0.045
0.517CysTyr: 0.517 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.588AspAla: 4.588 ± 0.274
0.888AspCys: 0.888 ± 0.12
5.078AspAsp: 5.078 ± 0.268
5.741AspGlu: 5.741 ± 0.324
3.341AspPhe: 3.341 ± 0.221
4.932AspGly: 4.932 ± 0.349
1.445AspHis: 1.445 ± 0.146
4.667AspIle: 4.667 ± 0.238
4.044AspLys: 4.044 ± 0.238
4.919AspLeu: 4.919 ± 0.23
1.949AspMet: 1.949 ± 0.184
3.381AspAsn: 3.381 ± 0.206
2.267AspPro: 2.267 ± 0.226
1.538AspGln: 1.538 ± 0.122
2.784AspArg: 2.784 ± 0.2
4.256AspSer: 4.256 ± 0.25
3.54AspThr: 3.54 ± 0.222
5.29AspVal: 5.29 ± 0.254
1.445AspTrp: 1.445 ± 0.153
3.447AspTyr: 3.447 ± 0.244
0.0AspXaa: 0.0 ± 0.0
Glu
5.304GluAla: 5.304 ± 0.279
0.782GluCys: 0.782 ± 0.128
4.137GluAsp: 4.137 ± 0.267
5.635GluGlu: 5.635 ± 0.332
3.713GluPhe: 3.713 ± 0.219
2.997GluGly: 2.997 ± 0.182
2.029GluHis: 2.029 ± 0.155
5.688GluIle: 5.688 ± 0.283
4.999GluLys: 4.999 ± 0.337
7.584GluLeu: 7.584 ± 0.345
2.174GluMet: 2.174 ± 0.174
3.5GluAsn: 3.5 ± 0.221
1.803GluPro: 1.803 ± 0.152
3.023GluGln: 3.023 ± 0.203
4.614GluArg: 4.614 ± 0.277
4.23GluSer: 4.23 ± 0.217
4.508GluThr: 4.508 ± 0.233
4.508GluVal: 4.508 ± 0.229
0.994GluTrp: 0.994 ± 0.118
3.593GluTyr: 3.593 ± 0.22
0.0GluXaa: 0.0 ± 0.0
Phe
2.705PheAla: 2.705 ± 0.192
0.464PheCys: 0.464 ± 0.078
4.004PheAsp: 4.004 ± 0.223
3.726PheGlu: 3.726 ± 0.234
1.392PhePhe: 1.392 ± 0.169
2.745PheGly: 2.745 ± 0.179
0.994PheHis: 0.994 ± 0.098
3.103PheIle: 3.103 ± 0.202
2.758PheLys: 2.758 ± 0.228
2.241PheLeu: 2.241 ± 0.161
1.299PheMet: 1.299 ± 0.135
2.586PheAsn: 2.586 ± 0.191
1.167PhePro: 1.167 ± 0.136
1.114PheGln: 1.114 ± 0.133
1.737PheArg: 1.737 ± 0.14
2.586PheSer: 2.586 ± 0.162
2.877PheThr: 2.877 ± 0.225
2.771PheVal: 2.771 ± 0.219
0.583PheTrp: 0.583 ± 0.101
1.565PheTyr: 1.565 ± 0.134
0.0PheXaa: 0.0 ± 0.0
Gly
3.726GlyAla: 3.726 ± 0.276
1.021GlyCys: 1.021 ± 0.12
4.574GlyAsp: 4.574 ± 0.324
4.137GlyGlu: 4.137 ± 0.221
2.771GlyPhe: 2.771 ± 0.204
4.071GlyGly: 4.071 ± 0.323
1.207GlyHis: 1.207 ± 0.124
3.819GlyIle: 3.819 ± 0.25
4.124GlyLys: 4.124 ± 0.272
4.057GlyLeu: 4.057 ± 0.22
1.936GlyMet: 1.936 ± 0.159
3.341GlyAsn: 3.341 ± 0.312
0.796GlyPro: 0.796 ± 0.109
1.909GlyGln: 1.909 ± 0.183
2.731GlyArg: 2.731 ± 0.176
4.495GlySer: 4.495 ± 0.403
3.858GlyThr: 3.858 ± 0.268
4.521GlyVal: 4.521 ± 0.236
1.18GlyTrp: 1.18 ± 0.144
2.784GlyTyr: 2.784 ± 0.21
0.0GlyXaa: 0.0 ± 0.0
His
1.697HisAla: 1.697 ± 0.156
0.345HisCys: 0.345 ± 0.064
1.644HisAsp: 1.644 ± 0.142
1.803HisGlu: 1.803 ± 0.166
1.008HisPhe: 1.008 ± 0.115
1.697HisGly: 1.697 ± 0.159
0.491HisHis: 0.491 ± 0.081
1.472HisIle: 1.472 ± 0.163
1.352HisLys: 1.352 ± 0.125
1.551HisLeu: 1.551 ± 0.153
0.663HisMet: 0.663 ± 0.135
0.968HisAsn: 0.968 ± 0.107
1.074HisPro: 1.074 ± 0.122
0.623HisGln: 0.623 ± 0.093
0.955HisArg: 0.955 ± 0.11
1.061HisSer: 1.061 ± 0.118
1.551HisThr: 1.551 ± 0.162
1.657HisVal: 1.657 ± 0.158
0.385HisTrp: 0.385 ± 0.073
0.955HisTyr: 0.955 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
5.131IleAla: 5.131 ± 0.295
0.663IleCys: 0.663 ± 0.092
5.794IleAsp: 5.794 ± 0.253
5.967IleGlu: 5.967 ± 0.262
2.36IlePhe: 2.36 ± 0.177
3.699IleGly: 3.699 ± 0.245
1.485IleHis: 1.485 ± 0.127
3.66IleIle: 3.66 ± 0.223
4.548IleLys: 4.548 ± 0.307
3.832IleLeu: 3.832 ± 0.283
1.87IleMet: 1.87 ± 0.156
3.898IleAsn: 3.898 ± 0.275
2.161IlePro: 2.161 ± 0.183
2.161IleGln: 2.161 ± 0.181
3.169IleArg: 3.169 ± 0.204
3.567IleSer: 3.567 ± 0.224
4.124IleThr: 4.124 ± 0.238
4.68IleVal: 4.68 ± 0.236
0.583IleTrp: 0.583 ± 0.084
2.082IleTyr: 2.082 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
4.932LysAla: 4.932 ± 0.319
0.729LysCys: 0.729 ± 0.099
3.925LysAsp: 3.925 ± 0.239
4.641LysGlu: 4.641 ± 0.355
2.599LysPhe: 2.599 ± 0.174
3.302LysGly: 3.302 ± 0.224
1.803LysHis: 1.803 ± 0.173
4.163LysIle: 4.163 ± 0.266
5.038LysLys: 5.038 ± 0.377
5.542LysLeu: 5.542 ± 0.32
2.174LysMet: 2.174 ± 0.181
2.93LysAsn: 2.93 ± 0.224
2.387LysPro: 2.387 ± 0.191
2.559LysGln: 2.559 ± 0.189
4.084LysArg: 4.084 ± 0.273
3.805LysSer: 3.805 ± 0.235
3.766LysThr: 3.766 ± 0.244
4.031LysVal: 4.031 ± 0.258
0.981LysTrp: 0.981 ± 0.098
2.89LysTyr: 2.89 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
5.264LeuAla: 5.264 ± 0.251
0.941LeuCys: 0.941 ± 0.102
5.701LeuAsp: 5.701 ± 0.293
5.861LeuGlu: 5.861 ± 0.278
2.572LeuPhe: 2.572 ± 0.164
4.495LeuGly: 4.495 ± 0.253
1.657LeuHis: 1.657 ± 0.157
4.19LeuIle: 4.19 ± 0.247
5.118LeuLys: 5.118 ± 0.333
5.503LeuLeu: 5.503 ± 0.282
2.068LeuMet: 2.068 ± 0.205
4.402LeuAsn: 4.402 ± 0.265
2.718LeuPro: 2.718 ± 0.18
2.387LeuGln: 2.387 ± 0.179
4.203LeuArg: 4.203 ± 0.224
5.37LeuSer: 5.37 ± 0.286
4.429LeuThr: 4.429 ± 0.213
4.23LeuVal: 4.23 ± 0.284
0.822LeuTrp: 0.822 ± 0.11
3.089LeuTyr: 3.089 ± 0.167
0.0LeuXaa: 0.0 ± 0.0
Met
1.458MetAla: 1.458 ± 0.123
0.371MetCys: 0.371 ± 0.083
0.915MetAsp: 0.915 ± 0.134
1.021MetGlu: 1.021 ± 0.119
1.326MetPhe: 1.326 ± 0.129
1.22MetGly: 1.22 ± 0.146
0.557MetHis: 0.557 ± 0.091
2.334MetIle: 2.334 ± 0.198
2.625MetLys: 2.625 ± 0.217
2.121MetLeu: 2.121 ± 0.186
0.822MetMet: 0.822 ± 0.117
1.803MetAsn: 1.803 ± 0.153
1.127MetPro: 1.127 ± 0.118
1.299MetGln: 1.299 ± 0.145
1.445MetArg: 1.445 ± 0.135
2.586MetSer: 2.586 ± 0.19
2.082MetThr: 2.082 ± 0.163
1.034MetVal: 1.034 ± 0.113
0.225MetTrp: 0.225 ± 0.055
1.061MetTyr: 1.061 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
3.699AsnAla: 3.699 ± 0.288
0.583AsnCys: 0.583 ± 0.093
3.779AsnAsp: 3.779 ± 0.259
4.031AsnGlu: 4.031 ± 0.199
2.068AsnPhe: 2.068 ± 0.161
4.402AsnGly: 4.402 ± 0.295
0.915AsnHis: 0.915 ± 0.111
3.58AsnIle: 3.58 ± 0.222
3.328AsnLys: 3.328 ± 0.23
3.633AsnLeu: 3.633 ± 0.242
1.233AsnMet: 1.233 ± 0.11
2.997AsnAsn: 2.997 ± 0.251
1.976AsnPro: 1.976 ± 0.193
1.551AsnGln: 1.551 ± 0.132
2.493AsnArg: 2.493 ± 0.169
2.917AsnSer: 2.917 ± 0.195
2.851AsnThr: 2.851 ± 0.21
3.686AsnVal: 3.686 ± 0.254
0.623AsnTrp: 0.623 ± 0.078
2.174AsnTyr: 2.174 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
1.803ProAla: 1.803 ± 0.155
0.398ProCys: 0.398 ± 0.075
2.294ProAsp: 2.294 ± 0.173
2.784ProGlu: 2.784 ± 0.207
1.445ProPhe: 1.445 ± 0.133
1.538ProGly: 1.538 ± 0.184
0.862ProHis: 0.862 ± 0.114
1.79ProIle: 1.79 ± 0.165
1.538ProLys: 1.538 ± 0.16
2.281ProLeu: 2.281 ± 0.167
0.663ProMet: 0.663 ± 0.081
1.989ProAsn: 1.989 ± 0.168
0.636ProPro: 0.636 ± 0.108
1.034ProGln: 1.034 ± 0.104
1.286ProArg: 1.286 ± 0.134
2.214ProSer: 2.214 ± 0.175
1.962ProThr: 1.962 ± 0.163
2.174ProVal: 2.174 ± 0.15
0.345ProTrp: 0.345 ± 0.062
1.432ProTyr: 1.432 ± 0.154
0.0ProXaa: 0.0 ± 0.0
Gln
2.161GlnAla: 2.161 ± 0.171
0.358GlnCys: 0.358 ± 0.065
1.962GlnAsp: 1.962 ± 0.181
2.851GlnGlu: 2.851 ± 0.216
1.339GlnPhe: 1.339 ± 0.136
2.068GlnGly: 2.068 ± 0.199
0.703GlnHis: 0.703 ± 0.108
2.493GlnIle: 2.493 ± 0.181
2.387GlnLys: 2.387 ± 0.175
2.771GlnLeu: 2.771 ± 0.191
0.928GlnMet: 0.928 ± 0.113
1.75GlnAsn: 1.75 ± 0.173
1.167GlnPro: 1.167 ± 0.119
1.193GlnGln: 1.193 ± 0.141
1.79GlnArg: 1.79 ± 0.149
1.856GlnSer: 1.856 ± 0.17
1.989GlnThr: 1.989 ± 0.18
2.002GlnVal: 2.002 ± 0.186
0.57GlnTrp: 0.57 ± 0.088
1.379GlnTyr: 1.379 ± 0.121
0.0GlnXaa: 0.0 ± 0.0
Arg
3.288ArgAla: 3.288 ± 0.234
0.676ArgCys: 0.676 ± 0.096
3.195ArgAsp: 3.195 ± 0.213
3.805ArgGlu: 3.805 ± 0.247
2.254ArgPhe: 2.254 ± 0.16
2.997ArgGly: 2.997 ± 0.209
1.074ArgHis: 1.074 ± 0.123
3.408ArgIle: 3.408 ± 0.228
3.514ArgLys: 3.514 ± 0.27
3.938ArgLeu: 3.938 ± 0.23
1.299ArgMet: 1.299 ± 0.149
2.307ArgAsn: 2.307 ± 0.176
1.419ArgPro: 1.419 ± 0.13
1.498ArgGln: 1.498 ± 0.136
2.413ArgArg: 2.413 ± 0.165
3.182ArgSer: 3.182 ± 0.232
2.546ArgThr: 2.546 ± 0.184
3.527ArgVal: 3.527 ± 0.231
0.862ArgTrp: 0.862 ± 0.105
2.334ArgTyr: 2.334 ± 0.169
0.0ArgXaa: 0.0 ± 0.0
Ser
3.991SerAla: 3.991 ± 0.239
0.703SerCys: 0.703 ± 0.111
4.296SerAsp: 4.296 ± 0.266
4.177SerGlu: 4.177 ± 0.222
3.103SerPhe: 3.103 ± 0.214
4.707SerGly: 4.707 ± 0.339
1.538SerHis: 1.538 ± 0.133
4.097SerIle: 4.097 ± 0.259
3.819SerLys: 3.819 ± 0.244
4.667SerLeu: 4.667 ± 0.275
1.83SerMet: 1.83 ± 0.159
3.328SerAsn: 3.328 ± 0.276
1.737SerPro: 1.737 ± 0.142
1.989SerGln: 1.989 ± 0.145
2.983SerArg: 2.983 ± 0.192
4.694SerSer: 4.694 ± 0.401
4.203SerThr: 4.203 ± 0.244
4.455SerVal: 4.455 ± 0.251
0.623SerTrp: 0.623 ± 0.086
2.32SerTyr: 2.32 ± 0.173
0.0SerXaa: 0.0 ± 0.0
Thr
4.071ThrAla: 4.071 ± 0.332
0.703ThrCys: 0.703 ± 0.096
3.66ThrAsp: 3.66 ± 0.227
4.296ThrGlu: 4.296 ± 0.278
2.665ThrPhe: 2.665 ± 0.206
4.269ThrGly: 4.269 ± 0.306
1.286ThrHis: 1.286 ± 0.14
4.336ThrIle: 4.336 ± 0.264
3.142ThrLys: 3.142 ± 0.261
4.866ThrLeu: 4.866 ± 0.267
1.379ThrMet: 1.379 ± 0.128
2.864ThrAsn: 2.864 ± 0.214
2.559ThrPro: 2.559 ± 0.185
2.453ThrGln: 2.453 ± 0.205
2.771ThrArg: 2.771 ± 0.188
3.593ThrSer: 3.593 ± 0.268
3.593ThrThr: 3.593 ± 0.259
4.879ThrVal: 4.879 ± 0.263
0.689ThrTrp: 0.689 ± 0.105
2.559ThrTyr: 2.559 ± 0.209
0.0ThrXaa: 0.0 ± 0.0
Val
4.349ValAla: 4.349 ± 0.279
0.809ValCys: 0.809 ± 0.115
4.972ValAsp: 4.972 ± 0.26
5.211ValGlu: 5.211 ± 0.274
3.156ValPhe: 3.156 ± 0.203
3.514ValGly: 3.514 ± 0.238
1.644ValHis: 1.644 ± 0.155
4.23ValIle: 4.23 ± 0.263
4.707ValLys: 4.707 ± 0.27
4.985ValLeu: 4.985 ± 0.269
1.551ValMet: 1.551 ± 0.144
3.408ValAsn: 3.408 ± 0.245
2.042ValPro: 2.042 ± 0.137
2.453ValGln: 2.453 ± 0.171
3.302ValArg: 3.302 ± 0.196
4.415ValSer: 4.415 ± 0.246
4.243ValThr: 4.243 ± 0.249
4.641ValVal: 4.641 ± 0.251
0.862ValTrp: 0.862 ± 0.114
2.904ValTyr: 2.904 ± 0.221
0.0ValXaa: 0.0 ± 0.0
Trp
0.928TrpAla: 0.928 ± 0.111
0.133TrpCys: 0.133 ± 0.045
1.021TrpAsp: 1.021 ± 0.118
1.047TrpGlu: 1.047 ± 0.101
0.623TrpPhe: 0.623 ± 0.094
0.769TrpGly: 0.769 ± 0.106
0.371TrpHis: 0.371 ± 0.07
0.491TrpIle: 0.491 ± 0.073
0.849TrpLys: 0.849 ± 0.093
1.233TrpLeu: 1.233 ± 0.14
0.398TrpMet: 0.398 ± 0.08
0.729TrpAsn: 0.729 ± 0.095
0.119TrpPro: 0.119 ± 0.032
0.477TrpGln: 0.477 ± 0.085
0.782TrpArg: 0.782 ± 0.099
0.941TrpSer: 0.941 ± 0.141
0.636TrpThr: 0.636 ± 0.139
1.101TrpVal: 1.101 ± 0.119
0.186TrpTrp: 0.186 ± 0.046
0.888TrpTyr: 0.888 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.142TyrAla: 3.142 ± 0.194
0.597TyrCys: 0.597 ± 0.097
3.248TyrAsp: 3.248 ± 0.194
2.758TyrGlu: 2.758 ± 0.245
1.578TyrPhe: 1.578 ± 0.133
2.519TyrGly: 2.519 ± 0.222
1.167TyrHis: 1.167 ± 0.121
2.36TyrIle: 2.36 ± 0.155
2.957TyrLys: 2.957 ± 0.229
3.169TyrLeu: 3.169 ± 0.169
1.074TyrMet: 1.074 ± 0.109
2.334TyrAsn: 2.334 ± 0.175
1.047TyrPro: 1.047 ± 0.121
1.498TyrGln: 1.498 ± 0.136
1.909TyrArg: 1.909 ± 0.157
2.718TyrSer: 2.718 ± 0.209
2.89TyrThr: 2.89 ± 0.214
3.142TyrVal: 3.142 ± 0.178
0.716TyrTrp: 0.716 ± 0.112
1.485TyrTyr: 1.485 ± 0.154
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 385 proteins (75421 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski