Simian adenovirus 49
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2WTJ7|F2WTJ7_9ADEN E1B protein small T-antigen OS=Simian adenovirus 49 OX=995022 PE=3 SV=1
MM1 pKa = 7.0 KK2 pKa = 9.53 TWGLDD7 pKa = 3.14 CGLYY11 pKa = 9.18 PQEE14 pKa = 3.58 VDD16 pKa = 2.4 EE17 pKa = 4.49 WLRR20 pKa = 11.84 TEE22 pKa = 4.05 YY23 pKa = 10.86 CPTPGYY29 pKa = 10.42 YY30 pKa = 10.54 GEE32 pKa = 4.46 NLSLHH37 pKa = 6.87 DD38 pKa = 5.51 LYY40 pKa = 11.22 DD41 pKa = 4.31 IDD43 pKa = 4.54 VDD45 pKa = 4.07 APADD49 pKa = 3.66 GDD51 pKa = 4.01 EE52 pKa = 4.69 NEE54 pKa = 4.39 VPVNDD59 pKa = 5.0 FFPDD63 pKa = 3.6 SLLLAVDD70 pKa = 3.7 EE71 pKa = 5.48 GIEE74 pKa = 4.12 VDD76 pKa = 4.46 YY77 pKa = 10.27 PPPLDD82 pKa = 3.78 TPGEE86 pKa = 4.06 PSGSHH91 pKa = 6.62 FMPDD95 pKa = 3.13 LSAEE99 pKa = 4.13 EE100 pKa = 3.93 VDD102 pKa = 5.15 LYY104 pKa = 10.87 CHH106 pKa = 6.96 EE107 pKa = 5.83 DD108 pKa = 3.54 GFPPSDD114 pKa = 3.72 SEE116 pKa = 4.73 GEE118 pKa = 3.84 QSEE121 pKa = 4.17 ARR123 pKa = 11.84 EE124 pKa = 4.0 EE125 pKa = 3.95 RR126 pKa = 11.84 LMAEE130 pKa = 4.36 AAATGAAAAARR141 pKa = 11.84 RR142 pKa = 11.84 AWEE145 pKa = 4.02 EE146 pKa = 3.72 EE147 pKa = 4.28 EE148 pKa = 4.97 FRR150 pKa = 11.84 LDD152 pKa = 3.87 CPVLPGHH159 pKa = 6.75 GCASCDD165 pKa = 3.6 YY166 pKa = 10.14 HH167 pKa = 7.61 RR168 pKa = 11.84 KK169 pKa = 7.47 TSGFPEE175 pKa = 4.42 IMCSLCYY182 pKa = 10.31 LRR184 pKa = 11.84 AHH186 pKa = 6.23 GMFVYY191 pKa = 10.59 SPVSDD196 pKa = 4.25 AEE198 pKa = 4.36 GEE200 pKa = 4.05 PDD202 pKa = 3.28 STTGHH207 pKa = 5.96 SGGPGSPPKK216 pKa = 10.46 LHH218 pKa = 5.97 NTPPRR223 pKa = 11.84 NVPRR227 pKa = 11.84 PVPLRR232 pKa = 11.84 VSGVRR237 pKa = 11.84 RR238 pKa = 11.84 AAVEE242 pKa = 4.17 SLHH245 pKa = 7.17 DD246 pKa = 4.69 LIGGEE251 pKa = 4.18 EE252 pKa = 4.19 EE253 pKa = 4.24 QVVPLDD259 pKa = 4.32 LSAKK263 pKa = 9.66 RR264 pKa = 11.84 PRR266 pKa = 11.84 HH267 pKa = 4.71
Molecular weight: 29.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.151
IPC2_protein 4.317
IPC_protein 4.266
Toseland 4.113
ProMoST 4.38
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.101
Rodwell 4.113
Grimsley 4.012
Solomon 4.228
Lehninger 4.177
Nozaki 4.329
DTASelect 4.495
Thurlkill 4.113
EMBOSS 4.126
Sillero 4.393
Patrickios 2.804
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.283
Protein with the highest isoelectric point:
>tr|F2WTL0|F2WTL0_9ADEN Hexon protein OS=Simian adenovirus 49 OX=995022 GN=L3 PE=2 SV=1
MM1 pKa = 7.74 ALTCRR6 pKa = 11.84 VRR8 pKa = 11.84 IPVPGYY14 pKa = 9.27 RR15 pKa = 11.84 GRR17 pKa = 11.84 SHH19 pKa = 6.73 RR20 pKa = 11.84 RR21 pKa = 11.84 HH22 pKa = 5.65 RR23 pKa = 11.84 RR24 pKa = 11.84 GLAGRR29 pKa = 11.84 GLRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AVRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 MRR42 pKa = 11.84 GGVLPLLIPLIAAAIGAVPGIASVALQASRR72 pKa = 11.84 KK73 pKa = 7.38 NN74 pKa = 3.5
Molecular weight: 8.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.437
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.223
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.959
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
5
32
11246
74
1201
351.4
39.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.257 ± 0.503
1.858 ± 0.296
4.571 ± 0.321
5.887 ± 0.476
3.672 ± 0.203
6.216 ± 0.356
2.392 ± 0.243
2.908 ± 0.188
2.863 ± 0.242
10.857 ± 0.469
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.099 ± 0.204
3.779 ± 0.416
7.585 ± 0.432
4.482 ± 0.255
8.038 ± 0.686
6.598 ± 0.457
5.406 ± 0.436
6.705 ± 0.387
1.334 ± 0.11
3.495 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here