Cephus cinctus (Wheat stem sawfly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Cephoidea; Cephidae; Cephus

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L9LTS5|A0A3L9LTS5_CEPCN Gustatory receptor OS=Cephus cinctus OX=211228 GN=Gr8 PE=3 SV=1
MM1 pKa = 7.58LLYY4 pKa = 9.81VCCVFFLLGFGNSHH18 pKa = 5.85GVSDD22 pKa = 5.16EE23 pKa = 3.97MMEE26 pKa = 4.17MAKK29 pKa = 9.18MLHH32 pKa = 6.7DD33 pKa = 4.07SCVSEE38 pKa = 4.12TGVDD42 pKa = 2.96EE43 pKa = 5.16GLIEE47 pKa = 4.1KK48 pKa = 10.46CRR50 pKa = 11.84DD51 pKa = 3.19GVFTEE56 pKa = 4.88DD57 pKa = 4.84SNLKK61 pKa = 10.5CYY63 pKa = 9.97IKK65 pKa = 10.55CIMDD69 pKa = 3.55QVSGMTDD76 pKa = 3.04DD77 pKa = 5.1GEE79 pKa = 4.34VDD81 pKa = 3.69EE82 pKa = 4.73EE83 pKa = 4.88TVISMLPEE91 pKa = 4.06EE92 pKa = 4.84MQSEE96 pKa = 4.42TAPTIRR102 pKa = 11.84EE103 pKa = 4.21CGTVRR108 pKa = 11.84GSDD111 pKa = 3.34NCDD114 pKa = 2.75TAFQTHH120 pKa = 5.23KK121 pKa = 10.37CYY123 pKa = 10.13YY124 pKa = 10.01AKK126 pKa = 10.8NPEE129 pKa = 3.95NYY131 pKa = 10.13FLVV134 pKa = 3.87

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L9LSS8|A0A3L9LSS8_CEPCN Ionotropic receptor 136 OS=Cephus cinctus OX=211228 GN=Ir136 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.21ALEE5 pKa = 4.47GFSRR9 pKa = 11.84ILITFIVAVMVIVKK23 pKa = 10.36ADD25 pKa = 3.05IRR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.41CRR31 pKa = 11.84KK32 pKa = 9.84QSGVSWAALKK42 pKa = 10.48KK43 pKa = 10.45LRR45 pKa = 11.84AGDD48 pKa = 4.25FNQEE52 pKa = 3.82DD53 pKa = 4.34HH54 pKa = 7.4SVKK57 pKa = 10.54CYY59 pKa = 10.42LKK61 pKa = 10.86CFMVKK66 pKa = 10.25NGIMSEE72 pKa = 4.04DD73 pKa = 3.75NYY75 pKa = 10.86VDD77 pKa = 3.0VDD79 pKa = 3.25KK80 pKa = 11.18ALRR83 pKa = 11.84HH84 pKa = 5.9LPRR87 pKa = 11.84KK88 pKa = 9.07LQEE91 pKa = 3.79PSRR94 pKa = 11.84KK95 pKa = 9.05ILARR99 pKa = 11.84CKK101 pKa = 10.64DD102 pKa = 3.49SAGKK106 pKa = 10.06DD107 pKa = 3.4SCDD110 pKa = 3.79KK111 pKa = 10.87AFQIAKK117 pKa = 9.7CYY119 pKa = 9.93FKK121 pKa = 10.85SQPGILKK128 pKa = 10.08NVSFVV133 pKa = 3.12

Molecular weight: 15.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

160

0

160

71586

113

935

447.4

51.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.184 ± 0.104

2.277 ± 0.074

4.012 ± 0.097

4.655 ± 0.085

6.125 ± 0.11

4.23 ± 0.083

2.026 ± 0.052

9.143 ± 0.169

5.29 ± 0.106

11.762 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.08 ± 0.076

5.153 ± 0.102

2.853 ± 0.079

3.01 ± 0.063

4.567 ± 0.089

7.489 ± 0.1

6.251 ± 0.085

6.859 ± 0.087

1.449 ± 0.042

4.586 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski