Sandarakinorhabdus sp. LB1R16

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Glacieibacterium

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A552UFV9|A0A552UFV9_9SPHN Isoquinoline 1-oxidoreductase subunit OS=Sandarakinorhabdus sp. LB1R16 OX=2593303 GN=FMM06_02575 PE=4 SV=1
MM1 pKa = 7.07TKK3 pKa = 9.91IVGAALAAALLAGSASALTTVTVEE27 pKa = 3.78AAGVQNTTLATTSGVEE43 pKa = 4.14NFDD46 pKa = 3.75SLSGYY51 pKa = 6.72TFPYY55 pKa = 9.71VSTFGGSPWTGSFNGVLSTGADD77 pKa = 3.28AYY79 pKa = 10.67GGAGGTGRR87 pKa = 11.84YY88 pKa = 6.4EE89 pKa = 4.2TVQGEE94 pKa = 4.43QILTLSGPATDD105 pKa = 5.8YY106 pKa = 11.2FGLWASALDD115 pKa = 3.98GGNTVAFYY123 pKa = 11.36KK124 pKa = 10.73NGALIDD130 pKa = 4.29TISLVMVALDD140 pKa = 4.48DD141 pKa = 5.25SYY143 pKa = 11.85DD144 pKa = 3.78GNPNPPYY151 pKa = 9.89NGQDD155 pKa = 2.83IGEE158 pKa = 4.45KK159 pKa = 9.4YY160 pKa = 10.82AFFNFVVAGGYY171 pKa = 10.58DD172 pKa = 3.99EE173 pKa = 4.85VHH175 pKa = 7.3LIQNLGGGFEE185 pKa = 4.86LDD187 pKa = 3.3NVTVGTLAAVPEE199 pKa = 4.19PASWAMMIAGFALVGLGMRR218 pKa = 11.84RR219 pKa = 11.84RR220 pKa = 11.84SVAAAA225 pKa = 3.51

Molecular weight: 22.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A552UAI2|A0A552UAI2_9SPHN 50S ribosomal protein L27 OS=Sandarakinorhabdus sp. LB1R16 OX=2593303 GN=rpmA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.36GFRR19 pKa = 11.84TRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.01VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3247

0

3247

1057848

20

4429

325.8

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.681 ± 0.07

0.733 ± 0.014

5.985 ± 0.032

4.645 ± 0.036

3.516 ± 0.029

9.406 ± 0.057

1.815 ± 0.023

4.566 ± 0.028

2.581 ± 0.036

9.943 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.024

2.407 ± 0.042

5.443 ± 0.042

2.71 ± 0.02

7.451 ± 0.047

4.812 ± 0.034

5.832 ± 0.051

7.86 ± 0.031

1.399 ± 0.02

2.135 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski