Haloglomus irregulare
Average proteome isoelectric point is 5.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3668 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A554NE85|A0A554NE85_9EURY Transposase OS=Haloglomus irregulare OX=2234134 GN=DP107_00450 PE=4 SV=1
MM1 pKa = 7.54 SAQDD5 pKa = 4.9 DD6 pKa = 4.1 IEE8 pKa = 4.77 LPEE11 pKa = 4.22 TLSYY15 pKa = 10.71 HH16 pKa = 6.58 DD17 pKa = 2.99 WAAAVRR23 pKa = 11.84 EE24 pKa = 4.23 GQLLGQSCNDD34 pKa = 3.53 CDD36 pKa = 3.92 HH37 pKa = 6.72 VGGVPSGACQEE48 pKa = 4.37 CGGRR52 pKa = 11.84 DD53 pKa = 3.6 LTTVEE58 pKa = 4.85 LPTTGTVYY66 pKa = 10.73 TEE68 pKa = 3.95 TTINVPPEE76 pKa = 4.02 GFQEE80 pKa = 3.55 RR81 pKa = 11.84 GYY83 pKa = 10.52 QVVVVDD89 pKa = 5.01 LGDD92 pKa = 3.56 ARR94 pKa = 11.84 VMARR98 pKa = 11.84 IVGEE102 pKa = 4.13 HH103 pKa = 5.93 VDD105 pKa = 3.37 IGDD108 pKa = 4.06 DD109 pKa = 3.49 VSLSGFIDD117 pKa = 3.52 TDD119 pKa = 3.88 DD120 pKa = 4.3 DD121 pKa = 4.07 HH122 pKa = 7.22 PAPTFEE128 pKa = 5.54 PDD130 pKa = 3.13 DD131 pKa = 3.75
Molecular weight: 14.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.872
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.872
Patrickios 0.998
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A554NAJ8|A0A554NAJ8_9EURY Adenylate kinase OS=Haloglomus irregulare OX=2234134 GN=DP107_10180 PE=4 SV=1
MM1 pKa = 7.03 VARR4 pKa = 11.84 PEE6 pKa = 4.28 AIRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 GRR16 pKa = 11.84 RR17 pKa = 11.84 PQPRR21 pKa = 11.84 PRR23 pKa = 11.84 RR24 pKa = 11.84 PPPQRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PLRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 PPLRR41 pKa = 11.84 RR42 pKa = 11.84 PTPPPPTGALRR53 pKa = 11.84 PGPQGVNGRR62 pKa = 11.84 AWTRR66 pKa = 11.84 RR67 pKa = 11.84 PRR69 pKa = 11.84 PTPRR73 pKa = 4.25
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3668
0
3668
1061482
22
3378
289.4
31.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.79 ± 0.065
0.759 ± 0.014
8.436 ± 0.049
8.114 ± 0.057
3.115 ± 0.025
9.277 ± 0.047
2.015 ± 0.023
3.309 ± 0.032
1.506 ± 0.023
8.874 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.662 ± 0.018
2.108 ± 0.025
5.123 ± 0.034
2.344 ± 0.023
7.33 ± 0.047
5.088 ± 0.04
6.534 ± 0.04
9.031 ± 0.05
1.072 ± 0.016
2.513 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here