Erwinia phage phiEt88

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5AGE5|E5AGE5_9CAUD Uncharacterized protein OS=Erwinia phage phiEt88 OX=925984 GN=P88_00460 PE=4 SV=1
MM1 pKa = 7.29IAVDD5 pKa = 4.57EE6 pKa = 4.91FDD8 pKa = 4.78LSTIADD14 pKa = 4.83FIGHH18 pKa = 6.05NWSDD22 pKa = 3.7FIGFCEE28 pKa = 4.11EE29 pKa = 4.46CSDD32 pKa = 4.78DD33 pKa = 5.52GEE35 pKa = 6.1AEE37 pKa = 3.99AQRR40 pKa = 11.84LYY42 pKa = 10.98EE43 pKa = 4.64AIGGEE48 pKa = 4.17AA49 pKa = 3.67

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5AGF3|E5AGF3_9CAUD Uncharacterized protein OS=Erwinia phage phiEt88 OX=925984 GN=P88_00540 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.85VTIEE6 pKa = 4.25DD7 pKa = 3.92YY8 pKa = 11.09EE9 pKa = 4.7VIDD12 pKa = 4.3RR13 pKa = 11.84LSTDD17 pKa = 3.05RR18 pKa = 11.84RR19 pKa = 11.84WRR21 pKa = 11.84QRR23 pKa = 11.84FDD25 pKa = 2.96SLAYY29 pKa = 9.95QNKK32 pKa = 8.14QVEE35 pKa = 4.57AARR38 pKa = 11.84MEE40 pKa = 4.43VVYY43 pKa = 10.78ALDD46 pKa = 3.86DD47 pKa = 3.49MATARR52 pKa = 11.84RR53 pKa = 11.84LQVRR57 pKa = 11.84KK58 pKa = 10.22VRR60 pKa = 11.84IFRR63 pKa = 11.84AAQPARR69 pKa = 11.84GEE71 pKa = 4.19KK72 pKa = 10.3

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13505

49

658

198.6

21.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.182 ± 0.461

1.14 ± 0.155

6.22 ± 0.209

5.835 ± 0.305

3.658 ± 0.243

7.345 ± 0.404

1.629 ± 0.124

6.775 ± 0.349

5.946 ± 0.316

7.775 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.673 ± 0.159

4.88 ± 0.189

3.414 ± 0.177

3.702 ± 0.203

5.243 ± 0.26

7.767 ± 0.338

5.835 ± 0.286

6.138 ± 0.218

1.585 ± 0.146

3.258 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski