Amino acid dipepetide frequency for Erwinia phage phiEt88

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.701AlaAla: 9.701 ± 1.8
0.444AlaCys: 0.444 ± 0.191
6.22AlaAsp: 6.22 ± 0.705
5.258AlaGlu: 5.258 ± 0.653
3.258AlaPhe: 3.258 ± 0.457
7.257AlaGly: 7.257 ± 0.932
1.629AlaHis: 1.629 ± 0.285
5.998AlaIle: 5.998 ± 0.658
4.739AlaLys: 4.739 ± 0.654
8.59AlaLeu: 8.59 ± 0.706
2.74AlaMet: 2.74 ± 0.425
3.555AlaAsn: 3.555 ± 0.474
2.222AlaPro: 2.222 ± 0.323
3.11AlaGln: 3.11 ± 0.531
4.665AlaArg: 4.665 ± 0.742
6.22AlaSer: 6.22 ± 0.694
5.036AlaThr: 5.036 ± 0.549
6.517AlaVal: 6.517 ± 0.745
1.703AlaTrp: 1.703 ± 0.675
2.962AlaTyr: 2.962 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.815CysAla: 0.815 ± 0.264
0.222CysCys: 0.222 ± 0.141
0.741CysAsp: 0.741 ± 0.284
1.259CysGlu: 1.259 ± 0.292
0.518CysPhe: 0.518 ± 0.18
0.963CysGly: 0.963 ± 0.242
0.148CysHis: 0.148 ± 0.111
0.592CysIle: 0.592 ± 0.257
0.518CysLys: 0.518 ± 0.302
1.111CysLeu: 1.111 ± 0.274
0.148CysMet: 0.148 ± 0.096
0.666CysAsn: 0.666 ± 0.287
0.296CysPro: 0.296 ± 0.145
0.296CysGln: 0.296 ± 0.144
0.963CysArg: 0.963 ± 0.296
0.518CysSer: 0.518 ± 0.216
0.518CysThr: 0.518 ± 0.172
0.518CysVal: 0.518 ± 0.158
0.37CysTrp: 0.37 ± 0.153
0.222CysTyr: 0.222 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.11AspAla: 5.11 ± 0.646
0.963AspCys: 0.963 ± 0.221
4.369AspAsp: 4.369 ± 0.572
4.591AspGlu: 4.591 ± 0.701
2.592AspPhe: 2.592 ± 0.415
5.702AspGly: 5.702 ± 0.673
0.815AspHis: 0.815 ± 0.262
3.703AspIle: 3.703 ± 0.404
4.147AspLys: 4.147 ± 0.502
4.295AspLeu: 4.295 ± 0.391
1.925AspMet: 1.925 ± 0.426
3.406AspAsn: 3.406 ± 0.495
0.889AspPro: 0.889 ± 0.262
1.111AspGln: 1.111 ± 0.317
2.444AspArg: 2.444 ± 0.477
4.961AspSer: 4.961 ± 0.641
3.48AspThr: 3.48 ± 0.588
3.703AspVal: 3.703 ± 0.4
0.815AspTrp: 0.815 ± 0.253
3.184AspTyr: 3.184 ± 0.524
0.0AspXaa: 0.0 ± 0.0
Glu
5.998GluAla: 5.998 ± 0.76
0.741GluCys: 0.741 ± 0.212
2.148GluAsp: 2.148 ± 0.308
2.37GluGlu: 2.37 ± 0.422
2.962GluPhe: 2.962 ± 0.529
2.888GluGly: 2.888 ± 0.428
1.185GluHis: 1.185 ± 0.339
5.258GluIle: 5.258 ± 0.626
4.221GluLys: 4.221 ± 0.579
4.887GluLeu: 4.887 ± 0.571
1.703GluMet: 1.703 ± 0.305
2.518GluAsn: 2.518 ± 0.368
1.481GluPro: 1.481 ± 0.306
2.666GluGln: 2.666 ± 0.477
3.184GluArg: 3.184 ± 0.454
3.851GluSer: 3.851 ± 0.508
3.036GluThr: 3.036 ± 0.456
3.999GluVal: 3.999 ± 0.507
1.629GluTrp: 1.629 ± 0.367
1.629GluTyr: 1.629 ± 0.369
0.0GluXaa: 0.0 ± 0.0
Phe
2.073PheAla: 2.073 ± 0.366
1.111PheCys: 1.111 ± 0.295
2.962PheAsp: 2.962 ± 0.461
1.481PheGlu: 1.481 ± 0.299
1.259PhePhe: 1.259 ± 0.298
2.444PheGly: 2.444 ± 0.401
0.37PheHis: 0.37 ± 0.153
2.222PheIle: 2.222 ± 0.453
1.555PheLys: 1.555 ± 0.313
2.666PheLeu: 2.666 ± 0.46
1.185PheMet: 1.185 ± 0.29
2.222PheAsn: 2.222 ± 0.438
1.259PhePro: 1.259 ± 0.3
1.259PheGln: 1.259 ± 0.329
2.592PheArg: 2.592 ± 0.371
4.369PheSer: 4.369 ± 0.683
2.296PheThr: 2.296 ± 0.443
2.073PheVal: 2.073 ± 0.455
0.222PheTrp: 0.222 ± 0.122
0.963PheTyr: 0.963 ± 0.32
0.0PheXaa: 0.0 ± 0.0
Gly
6.294GlyAla: 6.294 ± 1.175
0.666GlyCys: 0.666 ± 0.272
4.221GlyAsp: 4.221 ± 0.699
4.813GlyGlu: 4.813 ± 0.573
2.74GlyPhe: 2.74 ± 0.398
5.85GlyGly: 5.85 ± 0.933
0.741GlyHis: 0.741 ± 0.226
5.85GlyIle: 5.85 ± 0.556
5.184GlyLys: 5.184 ± 0.553
5.924GlyLeu: 5.924 ± 0.675
2.296GlyMet: 2.296 ± 0.337
3.851GlyAsn: 3.851 ± 0.655
1.185GlyPro: 1.185 ± 0.28
2.222GlyGln: 2.222 ± 0.41
3.48GlyArg: 3.48 ± 0.526
4.887GlySer: 4.887 ± 0.74
3.925GlyThr: 3.925 ± 0.599
5.554GlyVal: 5.554 ± 0.654
1.259GlyTrp: 1.259 ± 0.292
2.518GlyTyr: 2.518 ± 0.522
0.0GlyXaa: 0.0 ± 0.0
His
1.185HisAla: 1.185 ± 0.303
0.444HisCys: 0.444 ± 0.205
1.481HisAsp: 1.481 ± 0.36
1.111HisGlu: 1.111 ± 0.285
0.592HisPhe: 0.592 ± 0.19
1.111HisGly: 1.111 ± 0.324
0.37HisHis: 0.37 ± 0.204
0.815HisIle: 0.815 ± 0.252
0.963HisLys: 0.963 ± 0.26
1.185HisLeu: 1.185 ± 0.288
0.518HisMet: 0.518 ± 0.168
1.185HisAsn: 1.185 ± 0.289
0.666HisPro: 0.666 ± 0.2
0.444HisGln: 0.444 ± 0.217
0.518HisArg: 0.518 ± 0.233
1.777HisSer: 1.777 ± 0.446
0.815HisThr: 0.815 ± 0.207
0.592HisVal: 0.592 ± 0.202
0.148HisTrp: 0.148 ± 0.112
0.37HisTyr: 0.37 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
6.887IleAla: 6.887 ± 0.803
0.592IleCys: 0.592 ± 0.266
5.11IleAsp: 5.11 ± 0.664
3.851IleGlu: 3.851 ± 0.615
2.296IlePhe: 2.296 ± 0.327
4.147IleGly: 4.147 ± 0.616
1.481IleHis: 1.481 ± 0.318
4.147IleIle: 4.147 ± 0.788
4.887IleLys: 4.887 ± 0.659
3.258IleLeu: 3.258 ± 0.721
0.815IleMet: 0.815 ± 0.203
3.851IleAsn: 3.851 ± 0.5
2.962IlePro: 2.962 ± 0.447
1.925IleGln: 1.925 ± 0.401
3.703IleArg: 3.703 ± 0.446
5.924IleSer: 5.924 ± 0.815
5.48IleThr: 5.48 ± 0.589
3.184IleVal: 3.184 ± 0.479
1.185IleTrp: 1.185 ± 0.239
2.073IleTyr: 2.073 ± 0.435
0.0IleXaa: 0.0 ± 0.0
Lys
5.628LysAla: 5.628 ± 0.772
0.37LysCys: 0.37 ± 0.153
2.296LysAsp: 2.296 ± 0.366
3.184LysGlu: 3.184 ± 0.392
1.703LysPhe: 1.703 ± 0.353
3.703LysGly: 3.703 ± 0.5
1.037LysHis: 1.037 ± 0.289
3.629LysIle: 3.629 ± 0.586
4.147LysLys: 4.147 ± 0.642
5.406LysLeu: 5.406 ± 0.605
2.296LysMet: 2.296 ± 0.348
3.925LysAsn: 3.925 ± 0.536
2.888LysPro: 2.888 ± 0.546
3.036LysGln: 3.036 ± 0.57
2.592LysArg: 2.592 ± 0.456
3.999LysSer: 3.999 ± 0.538
3.406LysThr: 3.406 ± 0.474
3.48LysVal: 3.48 ± 0.459
0.666LysTrp: 0.666 ± 0.251
2.073LysTyr: 2.073 ± 0.384
0.0LysXaa: 0.0 ± 0.0
Leu
7.924LeuAla: 7.924 ± 0.758
0.666LeuCys: 0.666 ± 0.226
5.184LeuAsp: 5.184 ± 0.567
4.147LeuGlu: 4.147 ± 0.555
2.37LeuPhe: 2.37 ± 0.504
4.665LeuGly: 4.665 ± 0.623
1.333LeuHis: 1.333 ± 0.279
5.406LeuIle: 5.406 ± 0.692
3.851LeuLys: 3.851 ± 0.557
5.48LeuLeu: 5.48 ± 0.672
2.073LeuMet: 2.073 ± 0.349
4.591LeuAsn: 4.591 ± 0.601
2.74LeuPro: 2.74 ± 0.493
2.814LeuGln: 2.814 ± 0.469
5.258LeuArg: 5.258 ± 0.543
6.887LeuSer: 6.887 ± 0.81
5.924LeuThr: 5.924 ± 0.578
3.48LeuVal: 3.48 ± 0.472
0.741LeuTrp: 0.741 ± 0.22
2.222LeuTyr: 2.222 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
3.036MetAla: 3.036 ± 0.436
0.37MetCys: 0.37 ± 0.16
1.481MetAsp: 1.481 ± 0.357
2.148MetGlu: 2.148 ± 0.406
0.518MetPhe: 0.518 ± 0.18
1.185MetGly: 1.185 ± 0.248
0.444MetHis: 0.444 ± 0.168
1.259MetIle: 1.259 ± 0.272
2.444MetLys: 2.444 ± 0.376
1.407MetLeu: 1.407 ± 0.346
0.518MetMet: 0.518 ± 0.203
1.703MetAsn: 1.703 ± 0.318
1.111MetPro: 1.111 ± 0.261
2.073MetGln: 2.073 ± 0.413
1.481MetArg: 1.481 ± 0.349
1.925MetSer: 1.925 ± 0.33
1.777MetThr: 1.777 ± 0.338
1.259MetVal: 1.259 ± 0.429
0.296MetTrp: 0.296 ± 0.12
0.296MetTyr: 0.296 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
4.739AsnAla: 4.739 ± 0.595
0.815AsnCys: 0.815 ± 0.228
2.962AsnAsp: 2.962 ± 0.404
3.184AsnGlu: 3.184 ± 0.481
1.629AsnPhe: 1.629 ± 0.282
4.147AsnGly: 4.147 ± 0.575
0.741AsnHis: 0.741 ± 0.183
2.888AsnIle: 2.888 ± 0.476
2.444AsnLys: 2.444 ± 0.461
3.925AsnLeu: 3.925 ± 0.523
1.259AsnMet: 1.259 ± 0.324
2.666AsnAsn: 2.666 ± 0.554
2.444AsnPro: 2.444 ± 0.394
1.259AsnGln: 1.259 ± 0.297
2.073AsnArg: 2.073 ± 0.336
4.295AsnSer: 4.295 ± 0.654
2.592AsnThr: 2.592 ± 0.4
2.148AsnVal: 2.148 ± 0.31
0.815AsnTrp: 0.815 ± 0.213
1.777AsnTyr: 1.777 ± 0.347
0.0AsnXaa: 0.0 ± 0.0
Pro
4.073ProAla: 4.073 ± 0.645
0.518ProCys: 0.518 ± 0.192
2.592ProAsp: 2.592 ± 0.385
2.296ProGlu: 2.296 ± 0.379
1.481ProPhe: 1.481 ± 0.323
3.332ProGly: 3.332 ± 0.538
0.592ProHis: 0.592 ± 0.199
2.073ProIle: 2.073 ± 0.389
1.111ProLys: 1.111 ± 0.256
2.592ProLeu: 2.592 ± 0.412
0.815ProMet: 0.815 ± 0.293
1.259ProAsn: 1.259 ± 0.352
1.407ProPro: 1.407 ± 0.331
0.815ProGln: 0.815 ± 0.244
1.555ProArg: 1.555 ± 0.381
1.703ProSer: 1.703 ± 0.279
1.333ProThr: 1.333 ± 0.293
2.962ProVal: 2.962 ± 0.54
0.666ProTrp: 0.666 ± 0.233
0.963ProTyr: 0.963 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
4.073GlnAla: 4.073 ± 0.532
0.148GlnCys: 0.148 ± 0.109
1.999GlnAsp: 1.999 ± 0.421
1.703GlnGlu: 1.703 ± 0.374
1.259GlnPhe: 1.259 ± 0.306
2.296GlnGly: 2.296 ± 0.388
0.37GlnHis: 0.37 ± 0.156
2.666GlnIle: 2.666 ± 0.511
2.74GlnLys: 2.74 ± 0.459
3.036GlnLeu: 3.036 ± 0.367
0.889GlnMet: 0.889 ± 0.254
1.407GlnAsn: 1.407 ± 0.395
1.333GlnPro: 1.333 ± 0.281
2.592GlnGln: 2.592 ± 0.559
2.073GlnArg: 2.073 ± 0.448
2.296GlnSer: 2.296 ± 0.547
1.629GlnThr: 1.629 ± 0.339
2.592GlnVal: 2.592 ± 0.486
0.815GlnTrp: 0.815 ± 0.221
1.037GlnTyr: 1.037 ± 0.238
0.0GlnXaa: 0.0 ± 0.0
Arg
3.703ArgAla: 3.703 ± 0.555
0.889ArgCys: 0.889 ± 0.256
2.962ArgAsp: 2.962 ± 0.446
3.777ArgGlu: 3.777 ± 0.653
2.296ArgPhe: 2.296 ± 0.411
2.666ArgGly: 2.666 ± 0.36
1.037ArgHis: 1.037 ± 0.265
4.147ArgIle: 4.147 ± 0.542
2.962ArgLys: 2.962 ± 0.458
5.11ArgLeu: 5.11 ± 0.532
1.481ArgMet: 1.481 ± 0.312
2.296ArgAsn: 2.296 ± 0.478
2.148ArgPro: 2.148 ± 0.341
2.148ArgGln: 2.148 ± 0.449
3.703ArgArg: 3.703 ± 0.519
2.666ArgSer: 2.666 ± 0.276
2.296ArgThr: 2.296 ± 0.433
3.777ArgVal: 3.777 ± 0.484
0.592ArgTrp: 0.592 ± 0.184
1.777ArgTyr: 1.777 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
6.294SerAla: 6.294 ± 0.628
0.741SerCys: 0.741 ± 0.27
5.184SerAsp: 5.184 ± 0.648
3.925SerGlu: 3.925 ± 0.483
2.592SerPhe: 2.592 ± 0.47
6.887SerGly: 6.887 ± 0.923
1.111SerHis: 1.111 ± 0.313
5.258SerIle: 5.258 ± 0.647
4.665SerLys: 4.665 ± 0.515
6.294SerLeu: 6.294 ± 0.678
1.703SerMet: 1.703 ± 0.369
2.518SerAsn: 2.518 ± 0.581
2.592SerPro: 2.592 ± 0.595
3.036SerGln: 3.036 ± 0.489
3.48SerArg: 3.48 ± 0.508
6.961SerSer: 6.961 ± 1.117
4.887SerThr: 4.887 ± 0.596
5.48SerVal: 5.48 ± 0.558
1.111SerTrp: 1.111 ± 0.262
2.962SerTyr: 2.962 ± 0.523
0.0SerXaa: 0.0 ± 0.0
Thr
5.48ThrAla: 5.48 ± 0.699
0.592ThrCys: 0.592 ± 0.282
4.665ThrAsp: 4.665 ± 0.586
3.11ThrGlu: 3.11 ± 0.509
2.444ThrPhe: 2.444 ± 0.414
5.998ThrGly: 5.998 ± 0.718
0.963ThrHis: 0.963 ± 0.234
3.925ThrIle: 3.925 ± 0.428
2.74ThrLys: 2.74 ± 0.507
4.295ThrLeu: 4.295 ± 0.504
1.777ThrMet: 1.777 ± 0.444
1.555ThrAsn: 1.555 ± 0.28
2.518ThrPro: 2.518 ± 0.426
2.073ThrGln: 2.073 ± 0.407
2.148ThrArg: 2.148 ± 0.379
4.961ThrSer: 4.961 ± 0.612
2.74ThrThr: 2.74 ± 0.441
3.777ThrVal: 3.777 ± 0.503
0.815ThrTrp: 0.815 ± 0.222
1.777ThrTyr: 1.777 ± 0.365
0.0ThrXaa: 0.0 ± 0.0
Val
3.851ValAla: 3.851 ± 0.58
0.518ValCys: 0.518 ± 0.171
3.332ValAsp: 3.332 ± 0.501
3.11ValGlu: 3.11 ± 0.471
2.148ValPhe: 2.148 ± 0.413
4.739ValGly: 4.739 ± 0.688
0.815ValHis: 0.815 ± 0.183
5.11ValIle: 5.11 ± 0.654
3.999ValLys: 3.999 ± 0.524
3.48ValLeu: 3.48 ± 0.447
1.925ValMet: 1.925 ± 0.367
3.629ValAsn: 3.629 ± 0.554
2.073ValPro: 2.073 ± 0.367
2.073ValGln: 2.073 ± 0.394
3.036ValArg: 3.036 ± 0.393
5.554ValSer: 5.554 ± 0.783
4.591ValThr: 4.591 ± 0.592
3.925ValVal: 3.925 ± 0.482
1.037ValTrp: 1.037 ± 0.227
2.444ValTyr: 2.444 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
1.999TrpAla: 1.999 ± 0.442
0.296TrpCys: 0.296 ± 0.136
0.741TrpAsp: 0.741 ± 0.312
0.666TrpGlu: 0.666 ± 0.188
0.592TrpPhe: 0.592 ± 0.184
1.111TrpGly: 1.111 ± 0.336
0.296TrpHis: 0.296 ± 0.139
0.592TrpIle: 0.592 ± 0.178
0.963TrpLys: 0.963 ± 0.222
1.703TrpLeu: 1.703 ± 0.391
0.37TrpMet: 0.37 ± 0.15
0.741TrpAsn: 0.741 ± 0.33
0.592TrpPro: 0.592 ± 0.216
0.518TrpGln: 0.518 ± 0.173
1.185TrpArg: 1.185 ± 0.263
1.259TrpSer: 1.259 ± 0.307
0.518TrpThr: 0.518 ± 0.191
0.666TrpVal: 0.666 ± 0.214
0.444TrpTrp: 0.444 ± 0.168
0.592TrpTyr: 0.592 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.962TyrAla: 2.962 ± 0.506
0.296TyrCys: 0.296 ± 0.129
1.555TyrAsp: 1.555 ± 0.305
2.37TyrGlu: 2.37 ± 0.385
1.333TyrPhe: 1.333 ± 0.318
2.37TyrGly: 2.37 ± 0.408
0.815TyrHis: 0.815 ± 0.271
2.222TyrIle: 2.222 ± 0.418
1.185TyrLys: 1.185 ± 0.262
3.11TyrLeu: 3.11 ± 0.399
0.222TyrMet: 0.222 ± 0.115
1.259TyrAsn: 1.259 ± 0.272
1.333TyrPro: 1.333 ± 0.325
1.555TyrGln: 1.555 ± 0.326
2.296TyrArg: 2.296 ± 0.398
2.666TyrSer: 2.666 ± 0.406
2.073TyrThr: 2.073 ± 0.355
1.703TyrVal: 1.703 ± 0.386
0.518TyrTrp: 0.518 ± 0.201
0.741TyrTyr: 0.741 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski