Bacteriovorax sp. Seq25_V
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3292 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0SM95|T0SM95_9PROT Sigma factor regulatory protein FecR/PupR family OS=Bacteriovorax sp. Seq25_V OX=1201288 GN=M900_2415 PE=4 SV=1
MM1 pKa = 7.99 RR2 pKa = 11.84 YY3 pKa = 9.21 ILLVLVSLSVEE14 pKa = 3.9 AMSVEE19 pKa = 4.53 VSWNDD24 pKa = 3.21 NFSKK28 pKa = 10.85 SLTLYY33 pKa = 10.93 CEE35 pKa = 4.56 GNNEE39 pKa = 4.01 CLTYY43 pKa = 10.27 FDD45 pKa = 4.47 EE46 pKa = 4.47 VTVIEE51 pKa = 4.89 EE52 pKa = 4.45 EE53 pKa = 4.26 LCNDD57 pKa = 4.1 CFSTDD62 pKa = 3.32 MTSSFIFNGIGEE74 pKa = 4.85 SITTGEE80 pKa = 4.28 LAVPLDD86 pKa = 4.12 FFDD89 pKa = 5.19 LLKK92 pKa = 11.17 SNNFVSIDD100 pKa = 3.48 YY101 pKa = 10.06 DD102 pKa = 3.61 SPYY105 pKa = 11.44 DD106 pKa = 3.72 LVTGQSRR113 pKa = 11.84 IKK115 pKa = 10.58 KK116 pKa = 9.06 KK117 pKa = 9.88 LAYY120 pKa = 10.11 RR121 pKa = 11.84 NLCDD125 pKa = 5.49 DD126 pKa = 3.98 SSADD130 pKa = 3.23 PVLFFEE136 pKa = 5.33 KK137 pKa = 10.49 DD138 pKa = 3.12 DD139 pKa = 4.13 FGRR142 pKa = 11.84 ISNPRR147 pKa = 11.84 FILCADD153 pKa = 3.85 GVYY156 pKa = 10.92 NLDD159 pKa = 4.2 LFSIDD164 pKa = 3.72 VNDD167 pKa = 4.46 DD168 pKa = 3.37 FFGIPEE174 pKa = 4.56 FYY176 pKa = 11.12
Molecular weight: 20.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.579
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.923
Patrickios 0.769
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|T0R9U3|T0R9U3_9PROT PBP family protein OS=Bacteriovorax sp. Seq25_V OX=1201288 GN=M900_0992 PE=4 SV=1
MM1 pKa = 7.47 AKK3 pKa = 9.97 KK4 pKa = 9.39 RR5 pKa = 11.84 TWQPKK10 pKa = 7.4 KK11 pKa = 9.93 KK12 pKa = 9.89 KK13 pKa = 9.33 RR14 pKa = 11.84 LRR16 pKa = 11.84 VHH18 pKa = 6.74 GFLKK22 pKa = 10.65 RR23 pKa = 11.84 MSTANGRR30 pKa = 11.84 KK31 pKa = 8.93 VVNARR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.55 GRR41 pKa = 11.84 KK42 pKa = 8.85 SLTVSTGKK50 pKa = 10.52 KK51 pKa = 9.6
Molecular weight: 5.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.492
IPC2_protein 11.155
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.778
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.501
IPC_peptide 13.364
IPC2_peptide 12.34
IPC2.peptide.svr19 9.062
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3292
0
3292
1059612
37
4791
321.9
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.047 ± 0.045
1.098 ± 0.016
5.74 ± 0.031
7.059 ± 0.049
5.278 ± 0.033
6.077 ± 0.047
1.83 ± 0.02
7.765 ± 0.042
8.277 ± 0.05
9.685 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.023
5.43 ± 0.033
3.13 ± 0.025
3.021 ± 0.024
3.803 ± 0.024
7.515 ± 0.046
5.271 ± 0.051
6.176 ± 0.032
0.83 ± 0.013
3.725 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here