Bacillus hemicellulosilyticus JCM 9152

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus hemicellulosilyticus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4QEQ1|W4QEQ1_9BACI rRNA methylase OS=Bacillus hemicellulosilyticus JCM 9152 OX=1236971 GN=JCM9152_1545 PE=4 SV=1
MM1 pKa = 6.95MKK3 pKa = 10.07KK4 pKa = 10.19GFVFIVLLTFMLVIVACNNSEE25 pKa = 4.2VTGEE29 pKa = 4.21EE30 pKa = 4.67GNDD33 pKa = 3.43SDD35 pKa = 6.04DD36 pKa = 3.63STNEE40 pKa = 3.82VEE42 pKa = 6.35NADD45 pKa = 3.59QDD47 pKa = 3.77QAADD51 pKa = 4.48DD52 pKa = 4.13EE53 pKa = 4.68AQNTDD58 pKa = 3.01GPVTVTFWHH67 pKa = 6.43AMGGALQEE75 pKa = 4.5ALDD78 pKa = 4.4EE79 pKa = 4.22LVNMYY84 pKa = 10.2NAAQDD89 pKa = 3.84DD90 pKa = 4.82VIVEE94 pKa = 4.16AEE96 pKa = 4.12YY97 pKa = 10.67QGSYY101 pKa = 11.29DD102 pKa = 3.46EE103 pKa = 5.51ALTKK107 pKa = 9.98FHH109 pKa = 6.95SVAGTDD115 pKa = 3.53SAPTMIQVFEE125 pKa = 4.42IGTMSMVHH133 pKa = 6.33SGHH136 pKa = 6.38ITPIQDD142 pKa = 4.88LIDD145 pKa = 4.26ADD147 pKa = 4.55GYY149 pKa = 11.67DD150 pKa = 3.65MSSLEE155 pKa = 4.35DD156 pKa = 3.85NIINYY161 pKa = 9.14YY162 pKa = 10.46KK163 pKa = 10.06IDD165 pKa = 3.66DD166 pKa = 4.03QFYY169 pKa = 10.74SMPFNSSTPVMYY181 pKa = 10.65YY182 pKa = 10.75NKK184 pKa = 10.28DD185 pKa = 3.16AFEE188 pKa = 4.36AAGLDD193 pKa = 3.61PDD195 pKa = 4.94APPEE199 pKa = 4.03TYY201 pKa = 10.61EE202 pKa = 4.32EE203 pKa = 4.15IEE205 pKa = 4.01EE206 pKa = 4.06ASRR209 pKa = 11.84QIVEE213 pKa = 4.9SNPDD217 pKa = 3.17MKK219 pKa = 11.03GFALQAYY226 pKa = 7.7GWLYY230 pKa = 10.57EE231 pKa = 4.13QLLANQGALLMNNDD245 pKa = 3.55NGRR248 pKa = 11.84NGTPTEE254 pKa = 4.09VGFTEE259 pKa = 4.96AEE261 pKa = 4.35GKK263 pKa = 10.06SVFAWVEE270 pKa = 3.66RR271 pKa = 11.84MIEE274 pKa = 4.13DD275 pKa = 3.88DD276 pKa = 3.41TFANYY281 pKa = 7.44GTNSDD286 pKa = 3.57NMVSGFMSGDD296 pKa = 2.87VAMFLQSSASVKK308 pKa = 10.49NVVEE312 pKa = 3.97NAPFEE317 pKa = 4.42VGVAFLPYY325 pKa = 10.08PEE327 pKa = 3.93NTEE330 pKa = 3.96RR331 pKa = 11.84NGVVIGGASLWMVDD345 pKa = 3.69GKK347 pKa = 10.26PEE349 pKa = 4.18AEE351 pKa = 5.5QMAAWDD357 pKa = 4.16FMKK360 pKa = 10.5FLQTPEE366 pKa = 4.39VQAQWHH372 pKa = 5.97VGTGYY377 pKa = 10.49FAINPAAYY385 pKa = 9.91DD386 pKa = 3.67EE387 pKa = 4.46EE388 pKa = 4.9VVLEE392 pKa = 4.67AYY394 pKa = 7.67EE395 pKa = 4.14TMPQLKK401 pKa = 8.25VTVDD405 pKa = 3.2QLQATTSNYY414 pKa = 7.76ATQGAIMDD422 pKa = 5.0MIPEE426 pKa = 4.09ARR428 pKa = 11.84RR429 pKa = 11.84ITEE432 pKa = 3.98TALEE436 pKa = 4.18TVYY439 pKa = 11.26NGGAVDD445 pKa = 3.98DD446 pKa = 4.9TYY448 pKa = 11.73QSVVDD453 pKa = 4.52QINAAIEE460 pKa = 3.89QANRR464 pKa = 11.84ARR466 pKa = 11.84GEE468 pKa = 3.89

Molecular weight: 51.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4QL20|W4QL20_9BACI Uncharacterized protein OS=Bacillus hemicellulosilyticus JCM 9152 OX=1236971 GN=JCM9152_3868 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4345

0

4345

1252832

37

3284

288.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.555 ± 0.036

0.703 ± 0.011

5.281 ± 0.038

7.891 ± 0.048

4.568 ± 0.036

6.54 ± 0.038

2.361 ± 0.018

7.713 ± 0.036

5.94 ± 0.037

9.771 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.023

4.272 ± 0.027

3.471 ± 0.023

4.149 ± 0.029

4.336 ± 0.025

6.161 ± 0.032

5.432 ± 0.027

7.166 ± 0.036

1.147 ± 0.015

3.69 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski