Amino acid dipepetide frequency for Bacillus hemicellulosilyticus JCM 9152

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.72AlaAla: 4.72 ± 0.075	0.583AlaCys: 0.583 ± 0.024	3.215AlaAsp: 3.215 ± 0.057	4.39AlaGlu: 4.39 ± 0.079	3.184AlaPhe: 3.184 ± 0.055	4.471AlaGly: 4.471 ± 0.071	1.397AlaHis: 1.397 ± 0.032	5.812AlaIle: 5.812 ± 0.073	4.019AlaLys: 4.019 ± 0.061	6.846AlaLeu: 6.846 ± 0.083	1.862AlaMet: 1.862 ± 0.043	2.888AlaAsn: 2.888 ± 0.05	1.878AlaPro: 1.878 ± 0.037	2.279AlaGln: 2.279 ± 0.043	2.574AlaArg: 2.574 ± 0.046	3.98AlaSer: 3.98 ± 0.063	3.696AlaThr: 3.696 ± 0.058	4.838AlaVal: 4.838 ± 0.074	0.626AlaTrp: 0.626 ± 0.024	2.294AlaTyr: 2.294 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.398CysAla: 0.398 ± 0.019	0.09CysCys: 0.09 ± 0.009	0.375CysAsp: 0.375 ± 0.019	0.449CysGlu: 0.449 ± 0.018	0.317CysPhe: 0.317 ± 0.016	0.624CysGly: 0.624 ± 0.024	0.233CysHis: 0.233 ± 0.015	0.504CysIle: 0.504 ± 0.02	0.313CysLys: 0.313 ± 0.017	0.696CysLeu: 0.696 ± 0.025	0.217CysMet: 0.217 ± 0.013	0.268CysAsn: 0.268 ± 0.013	0.327CysPro: 0.327 ± 0.019	0.295CysGln: 0.295 ± 0.017	0.307CysArg: 0.307 ± 0.013	0.51CysSer: 0.51 ± 0.019	0.372CysThr: 0.372 ± 0.018	0.417CysVal: 0.417 ± 0.02	0.069CysTrp: 0.069 ± 0.007	0.25CysTyr: 0.25 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.049AspAla: 3.049 ± 0.058	0.342AspCys: 0.342 ± 0.017	2.873AspAsp: 2.873 ± 0.064	4.965AspGlu: 4.965 ± 0.073	2.371AspPhe: 2.371 ± 0.048	3.725AspGly: 3.725 ± 0.071	1.553AspHis: 1.553 ± 0.04	3.934AspIle: 3.934 ± 0.054	2.371AspLys: 2.371 ± 0.046	5.08AspLeu: 5.08 ± 0.071	1.353AspMet: 1.353 ± 0.032	1.684AspAsn: 1.684 ± 0.041	2.095AspPro: 2.095 ± 0.059	2.551AspGln: 2.551 ± 0.046	2.463AspArg: 2.463 ± 0.047	2.71AspSer: 2.71 ± 0.05	2.465AspThr: 2.465 ± 0.041	4.234AspVal: 4.234 ± 0.067	0.749AspTrp: 0.749 ± 0.027	2.24AspTyr: 2.24 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	5.425GluAla: 5.425 ± 0.077	0.397GluCys: 0.397 ± 0.018	4.187GluAsp: 4.187 ± 0.072	7.873GluGlu: 7.873 ± 0.109	2.528GluPhe: 2.528 ± 0.048	4.8GluGly: 4.8 ± 0.08	1.851GluHis: 1.851 ± 0.041	5.202GluIle: 5.202 ± 0.066	5.502GluLys: 5.502 ± 0.087	7.774GluLeu: 7.774 ± 0.073	2.46GluMet: 2.46 ± 0.044	3.382GluAsn: 3.382 ± 0.06	2.173GluPro: 2.173 ± 0.043	4.319GluGln: 4.319 ± 0.071	4.285GluArg: 4.285 ± 0.062	3.888GluSer: 3.888 ± 0.06	3.823GluThr: 3.823 ± 0.057	5.523GluVal: 5.523 ± 0.079	1.031GluTrp: 1.031 ± 0.032	2.489GluTyr: 2.489 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	2.957PheAla: 2.957 ± 0.054	0.33PheCys: 0.33 ± 0.017	2.419PheAsp: 2.419 ± 0.049	2.933PheGlu: 2.933 ± 0.046	2.485PhePhe: 2.485 ± 0.055	3.089PheGly: 3.089 ± 0.043	1.106PheHis: 1.106 ± 0.031	3.992PheIle: 3.992 ± 0.081	2.173PheLys: 2.173 ± 0.04	4.464PheLeu: 4.464 ± 0.063	1.201PheMet: 1.201 ± 0.03	1.932PheAsn: 1.932 ± 0.041	1.607PhePro: 1.607 ± 0.038	1.834PheGln: 1.834 ± 0.042	1.667PheArg: 1.667 ± 0.034	3.321PheSer: 3.321 ± 0.053	2.532PheThr: 2.532 ± 0.047	3.38PheVal: 3.38 ± 0.055	0.508PheTrp: 0.508 ± 0.019	1.748PheTyr: 1.748 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.455GlyAla: 4.455 ± 0.065	0.578GlyCys: 0.578 ± 0.025	3.344GlyAsp: 3.344 ± 0.068	4.806GlyGlu: 4.806 ± 0.071	3.393GlyPhe: 3.393 ± 0.058	4.38GlyGly: 4.38 ± 0.074	1.505GlyHis: 1.505 ± 0.037	5.246GlyIle: 5.246 ± 0.067	3.92GlyLys: 3.92 ± 0.053	6.335GlyLeu: 6.335 ± 0.069	2.047GlyMet: 2.047 ± 0.042	2.482GlyAsn: 2.482 ± 0.041	1.647GlyPro: 1.647 ± 0.042	2.48GlyGln: 2.48 ± 0.046	2.648GlyArg: 2.648 ± 0.05	3.857GlySer: 3.857 ± 0.066	3.724GlyThr: 3.724 ± 0.065	4.947GlyVal: 4.947 ± 0.061	0.84GlyTrp: 0.84 ± 0.025	2.77GlyTyr: 2.77 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.344HisAla: 1.344 ± 0.036	0.225HisCys: 0.225 ± 0.015	1.232HisAsp: 1.232 ± 0.038	1.616HisGlu: 1.616 ± 0.038	1.304HisPhe: 1.304 ± 0.032	1.386HisGly: 1.386 ± 0.033	0.791HisHis: 0.791 ± 0.029	1.787HisIle: 1.787 ± 0.041	0.946HisLys: 0.946 ± 0.026	2.448HisLeu: 2.448 ± 0.046	0.584HisMet: 0.584 ± 0.023	0.812HisAsn: 0.812 ± 0.028	1.136HisPro: 1.136 ± 0.031	1.016HisGln: 1.016 ± 0.03	0.974HisArg: 0.974 ± 0.026	1.592HisSer: 1.592 ± 0.035	1.256HisThr: 1.256 ± 0.032	1.722HisVal: 1.722 ± 0.041	0.301HisTrp: 0.301 ± 0.017	1.139HisTyr: 1.139 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.591IleAla: 5.591 ± 0.079	0.609IleCys: 0.609 ± 0.025	4.729IleAsp: 4.729 ± 0.066	6.317IleGlu: 6.317 ± 0.07	3.078IlePhe: 3.078 ± 0.055	5.797IleGly: 5.797 ± 0.077	1.912IleHis: 1.912 ± 0.047	5.733IleIle: 5.733 ± 0.096	4.013IleLys: 4.013 ± 0.061	6.481IleLeu: 6.481 ± 0.087	1.807IleMet: 1.807 ± 0.041	3.154IleAsn: 3.154 ± 0.055	3.196IlePro: 3.196 ± 0.05	3.139IleGln: 3.139 ± 0.054	3.361IleArg: 3.361 ± 0.057	4.926IleSer: 4.926 ± 0.072	4.184IleThr: 4.184 ± 0.067	5.792IleVal: 5.792 ± 0.068	0.761IleTrp: 0.761 ± 0.026	2.552IleTyr: 2.552 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	3.859LysAla: 3.859 ± 0.057	0.325LysCys: 0.325 ± 0.017	3.067LysAsp: 3.067 ± 0.054	6.142LysGlu: 6.142 ± 0.084	1.38LysPhe: 1.38 ± 0.034	4.031LysGly: 4.031 ± 0.055	1.377LysHis: 1.377 ± 0.033	3.245LysIle: 3.245 ± 0.06	5.172LysLys: 5.172 ± 0.084	4.977LysLeu: 4.977 ± 0.071	1.897LysMet: 1.897 ± 0.038	2.331LysAsn: 2.331 ± 0.047	1.98LysPro: 1.98 ± 0.046	3.202LysGln: 3.202 ± 0.053	3.533LysArg: 3.533 ± 0.054	3.17LysSer: 3.17 ± 0.057	2.735LysThr: 2.735 ± 0.044	4.38LysVal: 4.38 ± 0.064	0.838LysTrp: 0.838 ± 0.026	1.754LysTyr: 1.754 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	6.9LeuAla: 6.9 ± 0.094	0.716LeuCys: 0.716 ± 0.028	4.95LeuAsp: 4.95 ± 0.063	7.013LeuGlu: 7.013 ± 0.086	5.097LeuPhe: 5.097 ± 0.077	5.712LeuGly: 5.712 ± 0.075	2.182LeuHis: 2.182 ± 0.044	7.394LeuIle: 7.394 ± 0.103	5.828LeuLys: 5.828 ± 0.078	10.285LeuLeu: 10.285 ± 0.11	2.695LeuMet: 2.695 ± 0.042	4.255LeuAsn: 4.255 ± 0.063	3.831LeuPro: 3.831 ± 0.057	3.882LeuGln: 3.882 ± 0.064	3.865LeuArg: 3.865 ± 0.057	6.894LeuSer: 6.894 ± 0.088	5.745LeuThr: 5.745 ± 0.066	6.216LeuVal: 6.216 ± 0.08	0.861LeuTrp: 0.861 ± 0.028	3.392LeuTyr: 3.392 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.926MetAla: 1.926 ± 0.043	0.13MetCys: 0.13 ± 0.01	1.43MetAsp: 1.43 ± 0.029	1.911MetGlu: 1.911 ± 0.042	1.127MetPhe: 1.127 ± 0.033	1.584MetGly: 1.584 ± 0.033	0.415MetHis: 0.415 ± 0.017	2.49MetIle: 2.49 ± 0.044	2.452MetLys: 2.452 ± 0.041	2.631MetLeu: 2.631 ± 0.044	0.987MetMet: 0.987 ± 0.028	1.782MetAsn: 1.782 ± 0.034	0.926MetPro: 0.926 ± 0.03	0.881MetGln: 0.881 ± 0.032	1.181MetArg: 1.181 ± 0.031	1.88MetSer: 1.88 ± 0.042	1.835MetThr: 1.835 ± 0.042	1.855MetVal: 1.855 ± 0.037	0.224MetTrp: 0.224 ± 0.012	0.865MetTyr: 0.865 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.43AsnAla: 2.43 ± 0.043	0.251AsnCys: 0.251 ± 0.014	2.561AsnAsp: 2.561 ± 0.051	4.179AsnGlu: 4.179 ± 0.06	1.37AsnPhe: 1.37 ± 0.037	3.347AsnGly: 3.347 ± 0.066	1.169AsnHis: 1.169 ± 0.03	2.936AsnIle: 2.936 ± 0.058	2.434AsnLys: 2.434 ± 0.046	3.348AsnLeu: 3.348 ± 0.059	1.225AsnMet: 1.225 ± 0.034	1.817AsnAsn: 1.817 ± 0.043	1.924AsnPro: 1.924 ± 0.049	2.115AsnGln: 2.115 ± 0.042	2.215AsnArg: 2.215 ± 0.047	2.173AsnSer: 2.173 ± 0.046	1.968AsnThr: 1.968 ± 0.037	3.154AsnVal: 3.154 ± 0.051	0.59AsnTrp: 0.59 ± 0.024	1.509AsnTyr: 1.509 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	1.99ProAla: 1.99 ± 0.042	0.192ProCys: 0.192 ± 0.012	1.976ProAsp: 1.976 ± 0.046	2.738ProGlu: 2.738 ± 0.059	2.071ProPhe: 2.071 ± 0.045	2.033ProGly: 2.033 ± 0.048	0.884ProHis: 0.884 ± 0.027	3.002ProIle: 3.002 ± 0.045	1.808ProLys: 1.808 ± 0.037	3.513ProLeu: 3.513 ± 0.055	0.822ProMet: 0.822 ± 0.022	1.759ProAsn: 1.759 ± 0.038	1.003ProPro: 1.003 ± 0.031	1.108ProGln: 1.108 ± 0.036	1.054ProArg: 1.054 ± 0.027	2.271ProSer: 2.271 ± 0.042	2.114ProThr: 2.114 ± 0.05	2.52ProVal: 2.52 ± 0.049	0.384ProTrp: 0.384 ± 0.017	1.47ProTyr: 1.47 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.044GlnAla: 3.044 ± 0.057	0.231GlnCys: 0.231 ± 0.015	1.707GlnAsp: 1.707 ± 0.041	3.083GlnGlu: 3.083 ± 0.052	1.929GlnPhe: 1.929 ± 0.04	2.24GlnGly: 2.24 ± 0.05	0.9GlnHis: 0.9 ± 0.028	2.792GlnIle: 2.792 ± 0.044	2.43GlnLys: 2.43 ± 0.052	5.041GlnLeu: 5.041 ± 0.081	1.298GlnMet: 1.298 ± 0.036	1.599GlnAsn: 1.599 ± 0.034	1.263GlnPro: 1.263 ± 0.04	2.237GlnGln: 2.237 ± 0.07	1.856GlnArg: 1.856 ± 0.041	2.688GlnSer: 2.688 ± 0.052	2.274GlnThr: 2.274 ± 0.043	2.861GlnVal: 2.861 ± 0.049	0.538GlnTrp: 0.538 ± 0.025	1.483GlnTyr: 1.483 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.605ArgAla: 2.605 ± 0.054	0.271ArgCys: 0.271 ± 0.016	2.184ArgAsp: 2.184 ± 0.049	3.326ArgGlu: 3.326 ± 0.056	2.139ArgPhe: 2.139 ± 0.044	2.437ArgGly: 2.437 ± 0.042	0.958ArgHis: 0.958 ± 0.026	3.319ArgIle: 3.319 ± 0.053	3.091ArgLys: 3.091 ± 0.054	4.427ArgLeu: 4.427 ± 0.065	1.435ArgMet: 1.435 ± 0.035	1.99ArgAsn: 1.99 ± 0.043	1.359ArgPro: 1.359 ± 0.03	1.774ArgGln: 1.774 ± 0.044	2.003ArgArg: 2.003 ± 0.048	2.545ArgSer: 2.545 ± 0.049	2.355ArgThr: 2.355 ± 0.05	2.835ArgVal: 2.835 ± 0.051	0.507ArgTrp: 0.507 ± 0.019	1.803ArgTyr: 1.803 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.511SerAla: 3.511 ± 0.055	0.384SerCys: 0.384 ± 0.017	2.982SerAsp: 2.982 ± 0.053	4.273SerGlu: 4.273 ± 0.058	3.484SerPhe: 3.484 ± 0.05	4.143SerGly: 4.143 ± 0.067	1.43SerHis: 1.43 ± 0.038	5.432SerIle: 5.432 ± 0.078	3.321SerLys: 3.321 ± 0.055	6.369SerLeu: 6.369 ± 0.065	1.913SerMet: 1.913 ± 0.042	2.688SerAsn: 2.688 ± 0.052	2.081SerPro: 2.081 ± 0.043	2.253SerGln: 2.253 ± 0.041	2.34SerArg: 2.34 ± 0.045	4.118SerSer: 4.118 ± 0.07	3.344SerThr: 3.344 ± 0.05	4.253SerVal: 4.253 ± 0.058	0.734SerTrp: 0.734 ± 0.029	2.549SerTyr: 2.549 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.505ThrAla: 3.505 ± 0.057	0.384ThrCys: 0.384 ± 0.019	2.766ThrAsp: 2.766 ± 0.053	3.668ThrGlu: 3.668 ± 0.055	2.855ThrPhe: 2.855 ± 0.057	3.723ThrGly: 3.723 ± 0.058	1.135ThrHis: 1.135 ± 0.031	5.062ThrIle: 5.062 ± 0.06	2.984ThrLys: 2.984 ± 0.05	5.373ThrLeu: 5.373 ± 0.074	1.387ThrMet: 1.387 ± 0.032	2.749ThrAsn: 2.749 ± 0.05	2.056ThrPro: 2.056 ± 0.039	1.495ThrGln: 1.495 ± 0.036	1.862ThrArg: 1.862 ± 0.042	3.319ThrSer: 3.319 ± 0.061	3.02ThrThr: 3.02 ± 0.052	4.218ThrVal: 4.218 ± 0.073	0.595ThrTrp: 0.595 ± 0.024	2.166ThrTyr: 2.166 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	5.068ValAla: 5.068 ± 0.061	0.573ValCys: 0.573 ± 0.022	4.057ValAsp: 4.057 ± 0.064	5.44ValGlu: 5.44 ± 0.071	3.119ValPhe: 3.119 ± 0.057	4.737ValGly: 4.737 ± 0.072	1.517ValHis: 1.517 ± 0.035	5.923ValIle: 5.923 ± 0.072	4.046ValLys: 4.046 ± 0.061	6.74ValLeu: 6.74 ± 0.083	1.989ValMet: 1.989 ± 0.037	3.062ValAsn: 3.062 ± 0.052	2.502ValPro: 2.502 ± 0.045	2.631ValGln: 2.631 ± 0.046	2.863ValArg: 2.863 ± 0.047	4.689ValSer: 4.689 ± 0.068	4.378ValThr: 4.378 ± 0.062	5.219ValVal: 5.219 ± 0.074	0.695ValTrp: 0.695 ± 0.023	2.414ValTyr: 2.414 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.657TrpAla: 0.657 ± 0.022	0.107TrpCys: 0.107 ± 0.01	0.641TrpAsp: 0.641 ± 0.025	0.803TrpGlu: 0.803 ± 0.028	0.553TrpPhe: 0.553 ± 0.023	0.724TrpGly: 0.724 ± 0.026	0.271TrpHis: 0.271 ± 0.016	0.854TrpIle: 0.854 ± 0.028	0.706TrpLys: 0.706 ± 0.023	1.331TrpLeu: 1.331 ± 0.035	0.372TrpMet: 0.372 ± 0.016	0.623TrpAsn: 0.623 ± 0.025	0.299TrpPro: 0.299 ± 0.016	0.449TrpGln: 0.449 ± 0.021	0.506TrpArg: 0.506 ± 0.02	0.742TrpSer: 0.742 ± 0.026	0.56TrpThr: 0.56 ± 0.022	0.694TrpVal: 0.694 ± 0.023	0.164TrpTrp: 0.164 ± 0.013	0.418TrpTyr: 0.418 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.117TyrAla: 2.117 ± 0.047	0.312TyrCys: 0.312 ± 0.017	2.112TyrAsp: 2.112 ± 0.047	2.985TyrGlu: 2.985 ± 0.055	1.893TyrPhe: 1.893 ± 0.035	2.422TyrGly: 2.422 ± 0.051	1.023TyrHis: 1.023 ± 0.032	2.472TyrIle: 2.472 ± 0.041	1.867TyrLys: 1.867 ± 0.037	3.555TyrLeu: 3.555 ± 0.052	0.96TyrMet: 0.96 ± 0.026	1.461TyrAsn: 1.461 ± 0.032	1.428TyrPro: 1.428 ± 0.038	1.552TyrGln: 1.552 ± 0.038	1.806TyrArg: 1.806 ± 0.038	2.334TyrSer: 2.334 ± 0.05	1.945TyrThr: 1.945 ± 0.048	2.605TyrVal: 2.605 ± 0.05	0.455TyrTrp: 0.455 ± 0.02	1.596TyrTyr: 1.596 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4345 proteins (1252832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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