Arsukibacterium sp. MJ3
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M2VJZ6|A0A0M2VJZ6_9GAMM S-adenosylmethionine synthase OS=Arsukibacterium sp. MJ3 OX=1632859 GN=metK PE=3 SV=1
MM1 pKa = 7.54 QDD3 pKa = 3.23 LEE5 pKa = 4.54 LWSMDD10 pKa = 4.08 EE11 pKa = 4.37 LCDD14 pKa = 3.43 HH15 pKa = 6.95 AFDD18 pKa = 4.85 IFEE21 pKa = 4.62 EE22 pKa = 4.29 LAADD26 pKa = 3.92 NLNAADD32 pKa = 3.88 YY33 pKa = 11.23 NLYY36 pKa = 8.39 QQQYY40 pKa = 7.74 EE41 pKa = 3.92 QSGYY45 pKa = 10.47 VDD47 pKa = 3.3 MVIPGAEE54 pKa = 3.87 WVEE57 pKa = 3.86 LTMQDD62 pKa = 5.13 LEE64 pKa = 4.56 PEE66 pKa = 4.33 LHH68 pKa = 6.8 FEE70 pKa = 4.14 AQIGLTGSNGAADD83 pKa = 3.68 MVLARR88 pKa = 11.84 ILLSRR93 pKa = 11.84 EE94 pKa = 3.87 KK95 pKa = 10.51 HH96 pKa = 6.85 DD97 pKa = 4.49 SLCHH101 pKa = 5.06 AQWRR105 pKa = 11.84 GQQ107 pKa = 3.21
Molecular weight: 12.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.986
IPC_protein 3.923
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.757
Grimsley 3.656
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.19
Thurlkill 3.783
EMBOSS 3.821
Sillero 4.05
Patrickios 0.947
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.918
Protein with the highest isoelectric point:
>tr|A0A0M2VIM7|A0A0M2VIM7_9GAMM Ribosomal RNA small subunit methyltransferase G OS=Arsukibacterium sp. MJ3 OX=1632859 GN=gidB PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVLKK12 pKa = 10.46 RR13 pKa = 11.84 KK14 pKa = 9.37 RR15 pKa = 11.84 NHH17 pKa = 5.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.28 SGRR29 pKa = 11.84 QVLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.25 GRR40 pKa = 11.84 QRR42 pKa = 11.84 LSAA45 pKa = 4.04
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3329
0
3329
1092290
29
3426
328.1
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.219 ± 0.048
0.964 ± 0.015
5.084 ± 0.035
5.056 ± 0.041
4.015 ± 0.029
6.685 ± 0.04
2.215 ± 0.021
5.802 ± 0.033
4.338 ± 0.039
11.665 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.344 ± 0.024
4.09 ± 0.033
4.143 ± 0.024
5.919 ± 0.051
4.835 ± 0.03
6.314 ± 0.038
5.267 ± 0.029
6.704 ± 0.038
1.321 ± 0.021
3.019 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here