Valsa malicola
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10560 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A423X6L7|A0A423X6L7_9PEZI Mediator of RNA polymerase II transcription subunit 16 OS=Valsa malicola OX=356882 GN=MED16 PE=3 SV=1
MM1 pKa = 7.36 HH2 pKa = 8.34 LIRR5 pKa = 11.84 PVWAVGTALLLASSSTAQTSTNCDD29 pKa = 3.47 PTQTTCDD36 pKa = 3.75 PDD38 pKa = 3.45 EE39 pKa = 4.54 ALGMAINVDD48 pKa = 4.59 FTQGEE53 pKa = 4.69 VNSFTSSGGTPSYY66 pKa = 11.14 GSDD69 pKa = 2.71 GVTFTISGSGDD80 pKa = 3.3 APQLNSMFYY89 pKa = 10.58 IMFGKK94 pKa = 10.26 VDD96 pKa = 3.38 ITMKK100 pKa = 10.09 VAPGTGIVTSVVLQSDD116 pKa = 4.11 DD117 pKa = 3.97 LDD119 pKa = 4.73 EE120 pKa = 6.67 IDD122 pKa = 4.93 MEE124 pKa = 4.71 FLGSDD129 pKa = 3.55 DD130 pKa = 4.94 SKK132 pKa = 11.04 VQLMYY137 pKa = 10.61 FGKK140 pKa = 10.59 GDD142 pKa = 3.59 RR143 pKa = 11.84 ADD145 pKa = 3.67 NAVVIADD152 pKa = 4.05 APDD155 pKa = 3.55 NQSNFVTYY163 pKa = 10.02 TIDD166 pKa = 3.13 WSSDD170 pKa = 3.0 RR171 pKa = 11.84 IAWSVGGTTVRR182 pKa = 11.84 VLEE185 pKa = 4.3 STTYY189 pKa = 10.02 PDD191 pKa = 4.76 YY192 pKa = 11.32 YY193 pKa = 9.89 PQTPMQLKK201 pKa = 9.38 FGIWAGGDD209 pKa = 3.32 SGNTQGTIDD218 pKa = 3.6 WAGGATDD225 pKa = 3.96 YY226 pKa = 11.63 SSGPFSAVVQSVAVTDD242 pKa = 3.91 YY243 pKa = 10.55 STGSSYY249 pKa = 11.02 TYY251 pKa = 11.03 GDD253 pKa = 3.5 TSGTWGSIEE262 pKa = 4.12 SDD264 pKa = 2.96 GGEE267 pKa = 4.06 VNANAGEE274 pKa = 4.2 AATVTSVSGATSTDD288 pKa = 2.79 SSNFPGSGIGDD299 pKa = 3.61 GTSTVVASVNSMPSGWHH316 pKa = 4.25 MTSNGKK322 pKa = 8.89 IVPNSSTVVKK332 pKa = 9.73 PPHH335 pKa = 6.09 VLLLAGPLSFFIFVLCNGIWRR356 pKa = 4.18
Molecular weight: 37.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.656
EMBOSS 3.821
Sillero 3.961
Patrickios 1.189
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A423VVT6|A0A423VVT6_9PEZI MFS domain-containing protein OS=Valsa malicola OX=356882 GN=VMCG_08552 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.17 RR3 pKa = 11.84 TRR5 pKa = 11.84 RR6 pKa = 11.84 LEE8 pKa = 4.29 DD9 pKa = 3.18 DD10 pKa = 3.78 TAVLMVPIYY19 pKa = 9.97 DD20 pKa = 3.97 EE21 pKa = 4.36 RR22 pKa = 11.84 QQGADD27 pKa = 2.99 LLMFPVSDD35 pKa = 4.59 EE36 pKa = 4.1 SRR38 pKa = 11.84 QPQQPQQPQPQPQSQPPQQLPQKK61 pKa = 10.4 LPQQQQTPPQQPLPQYY77 pKa = 9.3 QSHH80 pKa = 5.63 VTPQQRR86 pKa = 11.84 QQPQQPQRR94 pKa = 11.84 IEE96 pKa = 4.14 TPTARR101 pKa = 11.84 PPTPPPKK108 pKa = 9.75 PQLPLEE114 pKa = 4.49 CEE116 pKa = 4.01 DD117 pKa = 3.36 WDD119 pKa = 5.35 DD120 pKa = 3.85 YY121 pKa = 12.03 LMWRR125 pKa = 11.84 RR126 pKa = 11.84 AAEE129 pKa = 4.05 AASLSDD135 pKa = 3.27 QQQQQQHH142 pKa = 5.95 GPSSSSRR149 pKa = 11.84 WRR151 pKa = 11.84 RR152 pKa = 11.84 RR153 pKa = 11.84 ILHH156 pKa = 5.54 QHH158 pKa = 5.47 QQHH161 pKa = 5.68 QPPTAPKK168 pKa = 9.85 EE169 pKa = 4.3 DD170 pKa = 3.33 ILRR173 pKa = 11.84 GFEE176 pKa = 3.69 EE177 pKa = 4.04 WKK179 pKa = 10.39 EE180 pKa = 3.68 YY181 pKa = 10.59 LAASRR186 pKa = 11.84 AASRR190 pKa = 11.84 RR191 pKa = 11.84 GGRR194 pKa = 11.84 PISIISTSTTTTTTGRR210 pKa = 11.84 SFKK213 pKa = 10.18 RR214 pKa = 11.84 SSMCSISSTKK224 pKa = 10.14 SGKK227 pKa = 8.83 PRR229 pKa = 11.84 SLRR232 pKa = 11.84 RR233 pKa = 11.84 CSRR236 pKa = 11.84 SRR238 pKa = 11.84 HH239 pKa = 4.16 LRR241 pKa = 11.84 AYY243 pKa = 9.72 YY244 pKa = 10.42 SEE246 pKa = 5.01 GSLSTARR253 pKa = 11.84 NNQRR257 pKa = 11.84 VDD259 pKa = 3.47 GPLKK263 pKa = 8.93 EE264 pKa = 4.45 TIVNVDD270 pKa = 3.21 HH271 pKa = 6.66 VPRR274 pKa = 11.84 GRR276 pKa = 11.84 PQFQPRR282 pKa = 11.84 QPTSHH287 pKa = 6.12 QSLRR291 pKa = 11.84 ASWVSEE297 pKa = 4.09 SCGPPPHH304 pKa = 7.16 PPPDD308 pKa = 3.85 KK309 pKa = 10.19 PLPALPPIIDD319 pKa = 4.01 SPVIDD324 pKa = 4.86 ASPIVDD330 pKa = 3.88 SFPVEE335 pKa = 3.69 RR336 pKa = 11.84 RR337 pKa = 11.84 AKK339 pKa = 9.83 RR340 pKa = 11.84 RR341 pKa = 11.84 NRR343 pKa = 11.84 SLLDD347 pKa = 3.07 EE348 pKa = 4.52 RR349 pKa = 11.84 EE350 pKa = 3.84 RR351 pKa = 11.84 KK352 pKa = 9.52 RR353 pKa = 11.84 FHH355 pKa = 6.2 RR356 pKa = 11.84 VYY358 pKa = 9.67 WHH360 pKa = 6.6
Molecular weight: 41.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.438
Toseland 10.599
ProMoST 10.35
Dawson 10.701
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.804
Grimsley 10.76
Solomon 10.847
Lehninger 10.804
Nozaki 10.599
DTASelect 10.438
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.496
IPC_peptide 10.847
IPC2_peptide 9.531
IPC2.peptide.svr19 8.658
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10560
0
10560
5240010
50
5565
496.2
54.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.905 ± 0.02
1.142 ± 0.007
6.009 ± 0.016
6.305 ± 0.023
3.545 ± 0.014
7.388 ± 0.021
2.352 ± 0.011
4.554 ± 0.014
4.752 ± 0.022
8.601 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.286 ± 0.009
3.58 ± 0.012
6.117 ± 0.026
3.96 ± 0.019
6.057 ± 0.022
7.96 ± 0.027
6.0 ± 0.02
6.286 ± 0.017
1.47 ± 0.009
2.729 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here