Valsa malicola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Valsaceae; Valsa

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A423X6L7|A0A423X6L7_9PEZI Mediator of RNA polymerase II transcription subunit 16 OS=Valsa malicola OX=356882 GN=MED16 PE=3 SV=1
MM1 pKa = 7.36HH2 pKa = 8.34LIRR5 pKa = 11.84PVWAVGTALLLASSSTAQTSTNCDD29 pKa = 3.47PTQTTCDD36 pKa = 3.75PDD38 pKa = 3.45EE39 pKa = 4.54ALGMAINVDD48 pKa = 4.59FTQGEE53 pKa = 4.69VNSFTSSGGTPSYY66 pKa = 11.14GSDD69 pKa = 2.71GVTFTISGSGDD80 pKa = 3.3APQLNSMFYY89 pKa = 10.58IMFGKK94 pKa = 10.26VDD96 pKa = 3.38ITMKK100 pKa = 10.09VAPGTGIVTSVVLQSDD116 pKa = 4.11DD117 pKa = 3.97LDD119 pKa = 4.73EE120 pKa = 6.67IDD122 pKa = 4.93MEE124 pKa = 4.71FLGSDD129 pKa = 3.55DD130 pKa = 4.94SKK132 pKa = 11.04VQLMYY137 pKa = 10.61FGKK140 pKa = 10.59GDD142 pKa = 3.59RR143 pKa = 11.84ADD145 pKa = 3.67NAVVIADD152 pKa = 4.05APDD155 pKa = 3.55NQSNFVTYY163 pKa = 10.02TIDD166 pKa = 3.13WSSDD170 pKa = 3.0RR171 pKa = 11.84IAWSVGGTTVRR182 pKa = 11.84VLEE185 pKa = 4.3STTYY189 pKa = 10.02PDD191 pKa = 4.76YY192 pKa = 11.32YY193 pKa = 9.89PQTPMQLKK201 pKa = 9.38FGIWAGGDD209 pKa = 3.32SGNTQGTIDD218 pKa = 3.6WAGGATDD225 pKa = 3.96YY226 pKa = 11.63SSGPFSAVVQSVAVTDD242 pKa = 3.91YY243 pKa = 10.55STGSSYY249 pKa = 11.02TYY251 pKa = 11.03GDD253 pKa = 3.5TSGTWGSIEE262 pKa = 4.12SDD264 pKa = 2.96GGEE267 pKa = 4.06VNANAGEE274 pKa = 4.2AATVTSVSGATSTDD288 pKa = 2.79SSNFPGSGIGDD299 pKa = 3.61GTSTVVASVNSMPSGWHH316 pKa = 4.25MTSNGKK322 pKa = 8.89IVPNSSTVVKK332 pKa = 9.73PPHH335 pKa = 6.09VLLLAGPLSFFIFVLCNGIWRR356 pKa = 4.18

Molecular weight:
37.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A423VVT6|A0A423VVT6_9PEZI MFS domain-containing protein OS=Valsa malicola OX=356882 GN=VMCG_08552 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.17RR3 pKa = 11.84TRR5 pKa = 11.84RR6 pKa = 11.84LEE8 pKa = 4.29DD9 pKa = 3.18DD10 pKa = 3.78TAVLMVPIYY19 pKa = 9.97DD20 pKa = 3.97EE21 pKa = 4.36RR22 pKa = 11.84QQGADD27 pKa = 2.99LLMFPVSDD35 pKa = 4.59EE36 pKa = 4.1SRR38 pKa = 11.84QPQQPQQPQPQPQSQPPQQLPQKK61 pKa = 10.4LPQQQQTPPQQPLPQYY77 pKa = 9.3QSHH80 pKa = 5.63VTPQQRR86 pKa = 11.84QQPQQPQRR94 pKa = 11.84IEE96 pKa = 4.14TPTARR101 pKa = 11.84PPTPPPKK108 pKa = 9.75PQLPLEE114 pKa = 4.49CEE116 pKa = 4.01DD117 pKa = 3.36WDD119 pKa = 5.35DD120 pKa = 3.85YY121 pKa = 12.03LMWRR125 pKa = 11.84RR126 pKa = 11.84AAEE129 pKa = 4.05AASLSDD135 pKa = 3.27QQQQQQHH142 pKa = 5.95GPSSSSRR149 pKa = 11.84WRR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84ILHH156 pKa = 5.54QHH158 pKa = 5.47QQHH161 pKa = 5.68QPPTAPKK168 pKa = 9.85EE169 pKa = 4.3DD170 pKa = 3.33ILRR173 pKa = 11.84GFEE176 pKa = 3.69EE177 pKa = 4.04WKK179 pKa = 10.39EE180 pKa = 3.68YY181 pKa = 10.59LAASRR186 pKa = 11.84AASRR190 pKa = 11.84RR191 pKa = 11.84GGRR194 pKa = 11.84PISIISTSTTTTTTGRR210 pKa = 11.84SFKK213 pKa = 10.18RR214 pKa = 11.84SSMCSISSTKK224 pKa = 10.14SGKK227 pKa = 8.83PRR229 pKa = 11.84SLRR232 pKa = 11.84RR233 pKa = 11.84CSRR236 pKa = 11.84SRR238 pKa = 11.84HH239 pKa = 4.16LRR241 pKa = 11.84AYY243 pKa = 9.72YY244 pKa = 10.42SEE246 pKa = 5.01GSLSTARR253 pKa = 11.84NNQRR257 pKa = 11.84VDD259 pKa = 3.47GPLKK263 pKa = 8.93EE264 pKa = 4.45TIVNVDD270 pKa = 3.21HH271 pKa = 6.66VPRR274 pKa = 11.84GRR276 pKa = 11.84PQFQPRR282 pKa = 11.84QPTSHH287 pKa = 6.12QSLRR291 pKa = 11.84ASWVSEE297 pKa = 4.09SCGPPPHH304 pKa = 7.16PPPDD308 pKa = 3.85KK309 pKa = 10.19PLPALPPIIDD319 pKa = 4.01SPVIDD324 pKa = 4.86ASPIVDD330 pKa = 3.88SFPVEE335 pKa = 3.69RR336 pKa = 11.84RR337 pKa = 11.84AKK339 pKa = 9.83RR340 pKa = 11.84RR341 pKa = 11.84NRR343 pKa = 11.84SLLDD347 pKa = 3.07EE348 pKa = 4.52RR349 pKa = 11.84EE350 pKa = 3.84RR351 pKa = 11.84KK352 pKa = 9.52RR353 pKa = 11.84FHH355 pKa = 6.2RR356 pKa = 11.84VYY358 pKa = 9.67WHH360 pKa = 6.6

Molecular weight:
41.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10560

0

10560

5240010

50

5565

496.2

54.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.905 ± 0.02

1.142 ± 0.007

6.009 ± 0.016

6.305 ± 0.023

3.545 ± 0.014

7.388 ± 0.021

2.352 ± 0.011

4.554 ± 0.014

4.752 ± 0.022

8.601 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.009

3.58 ± 0.012

6.117 ± 0.026

3.96 ± 0.019

6.057 ± 0.022

7.96 ± 0.027

6.0 ± 0.02

6.286 ± 0.017

1.47 ± 0.009

2.729 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski