Arthrobacter phage OurGirlNessie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Korravirus; unclassified Korravirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9UG46|A0A3S9UG46_9CAUD Head-to-tail stopper OS=Arthrobacter phage OurGirlNessie OX=2499012 GN=8 PE=4 SV=1
MM1 pKa = 7.73ANFSYY6 pKa = 9.15YY7 pKa = 10.01TKK9 pKa = 10.82LALEE13 pKa = 5.01LSRR16 pKa = 11.84EE17 pKa = 4.02DD18 pKa = 3.35CGRR21 pKa = 11.84VVSIGNGEE29 pKa = 3.9WTIVGILHH37 pKa = 6.83RR38 pKa = 11.84VDD40 pKa = 4.54NEE42 pKa = 4.59DD43 pKa = 3.71IMEE46 pKa = 4.3LTSGYY51 pKa = 10.61DD52 pKa = 2.85WGGGGSRR59 pKa = 11.84SIRR62 pKa = 11.84CWVTVGPFKK71 pKa = 11.45GEE73 pKa = 3.58IDD75 pKa = 3.6PRR77 pKa = 11.84TQVTVEE83 pKa = 4.19VPALDD88 pKa = 4.15SGDD91 pKa = 3.81SGDD94 pKa = 3.98VIIGEE99 pKa = 4.19ILAA102 pKa = 4.47

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9UG77|A0A3S9UG77_9CAUD PurA-like adenylosuccinate synthetase OS=Arthrobacter phage OurGirlNessie OX=2499012 GN=45 PE=3 SV=1
MM1 pKa = 7.12EE2 pKa = 5.06RR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.46IAPRR9 pKa = 11.84ASSKK13 pKa = 11.1DD14 pKa = 3.6YY15 pKa = 10.51VRR17 pKa = 11.84KK18 pKa = 10.32GGAKK22 pKa = 9.31SVRR25 pKa = 11.84FTADD29 pKa = 2.52YY30 pKa = 9.82WSEE33 pKa = 3.63LRR35 pKa = 11.84KK36 pKa = 10.13RR37 pKa = 11.84KK38 pKa = 9.35EE39 pKa = 3.83DD40 pKa = 3.19QRR42 pKa = 11.84LRR44 pKa = 11.84LLEE47 pKa = 3.94RR48 pKa = 11.84AVINHH53 pKa = 5.47IRR55 pKa = 11.84FLGQDD60 pKa = 3.48GQAPHH65 pKa = 6.21YY66 pKa = 10.26VFFGGAHH73 pKa = 6.08EE74 pKa = 4.22ARR76 pKa = 11.84LWVDD80 pKa = 3.27EE81 pKa = 4.12FRR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84LDD87 pKa = 3.15VTGYY91 pKa = 10.79SFDD94 pKa = 3.72PRR96 pKa = 11.84KK97 pKa = 8.92QLSMARR103 pKa = 11.84PSRR106 pKa = 11.84LRR108 pKa = 11.84GISRR112 pKa = 11.84RR113 pKa = 11.84LVAVWAYY120 pKa = 11.13DD121 pKa = 3.48PMPVSDD127 pKa = 3.54RR128 pKa = 11.84EE129 pKa = 3.91YY130 pKa = 9.44RR131 pKa = 11.84AEE133 pKa = 4.05IEE135 pKa = 4.19TMHH138 pKa = 7.09VIEE141 pKa = 4.58RR142 pKa = 11.84QNRR145 pKa = 11.84LLGYY149 pKa = 9.89EE150 pKa = 3.93SGEE153 pKa = 4.15VVSRR157 pKa = 11.84EE158 pKa = 3.85TANAA162 pKa = 3.59

Molecular weight: 19.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13491

37

876

228.7

25.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.066 ± 0.3

0.801 ± 0.17

5.885 ± 0.231

6.508 ± 0.349

2.995 ± 0.225

8.784 ± 0.412

1.875 ± 0.163

4.633 ± 0.266

4.833 ± 0.329

8.45 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.145

3.536 ± 0.177

5.018 ± 0.17

4.077 ± 0.179

5.93 ± 0.413

6.234 ± 0.288

6.226 ± 0.502

7.242 ± 0.292

2.031 ± 0.185

2.535 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski