Amino acid dipepetide frequency for Arthrobacter phage OurGirlNessie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.268AlaAla: 11.268 ± 1.069
0.593AlaCys: 0.593 ± 0.213
5.189AlaAsp: 5.189 ± 0.482
7.265AlaGlu: 7.265 ± 0.883
2.817AlaPhe: 2.817 ± 0.497
8.525AlaGly: 8.525 ± 0.935
1.334AlaHis: 1.334 ± 0.332
4.448AlaIle: 4.448 ± 0.715
5.337AlaLys: 5.337 ± 0.794
9.192AlaLeu: 9.192 ± 1.15
2.001AlaMet: 2.001 ± 0.358
3.781AlaAsn: 3.781 ± 0.495
4.151AlaPro: 4.151 ± 0.584
5.115AlaGln: 5.115 ± 0.514
5.56AlaArg: 5.56 ± 0.67
6.079AlaSer: 6.079 ± 0.655
6.375AlaThr: 6.375 ± 0.604
6.523AlaVal: 6.523 ± 0.619
1.853AlaTrp: 1.853 ± 0.407
3.262AlaTyr: 3.262 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.371CysAla: 0.371 ± 0.156
0.148CysCys: 0.148 ± 0.103
0.371CysAsp: 0.371 ± 0.166
0.593CysGlu: 0.593 ± 0.219
0.074CysPhe: 0.074 ± 0.073
1.631CysGly: 1.631 ± 0.466
0.074CysHis: 0.074 ± 0.065
0.519CysIle: 0.519 ± 0.183
0.297CysLys: 0.297 ± 0.155
0.445CysLeu: 0.445 ± 0.185
0.074CysMet: 0.074 ± 0.065
0.445CysAsn: 0.445 ± 0.23
0.297CysPro: 0.297 ± 0.137
0.519CysGln: 0.519 ± 0.205
0.667CysArg: 0.667 ± 0.262
0.519CysSer: 0.519 ± 0.238
0.445CysThr: 0.445 ± 0.19
0.148CysVal: 0.148 ± 0.153
0.222CysTrp: 0.222 ± 0.137
0.148CysTyr: 0.148 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
5.263AspAla: 5.263 ± 0.61
0.667AspCys: 0.667 ± 0.289
3.41AspAsp: 3.41 ± 0.564
4.522AspGlu: 4.522 ± 0.531
2.001AspPhe: 2.001 ± 0.446
5.93AspGly: 5.93 ± 0.734
1.26AspHis: 1.26 ± 0.368
2.076AspIle: 2.076 ± 0.351
1.927AspLys: 1.927 ± 0.327
5.263AspLeu: 5.263 ± 0.608
1.334AspMet: 1.334 ± 0.291
1.557AspAsn: 1.557 ± 0.314
3.558AspPro: 3.558 ± 0.508
2.446AspGln: 2.446 ± 0.489
4.596AspArg: 4.596 ± 0.621
3.929AspSer: 3.929 ± 0.452
3.039AspThr: 3.039 ± 0.524
2.52AspVal: 2.52 ± 0.536
1.853AspTrp: 1.853 ± 0.373
1.631AspTyr: 1.631 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
8.377GluAla: 8.377 ± 0.995
0.667GluCys: 0.667 ± 0.258
4.374GluAsp: 4.374 ± 0.788
5.041GluGlu: 5.041 ± 0.612
1.853GluPhe: 1.853 ± 0.402
4.967GluGly: 4.967 ± 0.687
0.815GluHis: 0.815 ± 0.225
2.669GluIle: 2.669 ± 0.479
2.965GluLys: 2.965 ± 0.584
6.375GluLeu: 6.375 ± 0.638
1.26GluMet: 1.26 ± 0.414
2.224GluAsn: 2.224 ± 0.295
2.52GluPro: 2.52 ± 0.437
3.336GluGln: 3.336 ± 0.6
3.929GluArg: 3.929 ± 0.534
2.595GluSer: 2.595 ± 0.444
3.706GluThr: 3.706 ± 0.52
4.448GluVal: 4.448 ± 0.621
1.557GluTrp: 1.557 ± 0.387
1.408GluTyr: 1.408 ± 0.373
0.0GluXaa: 0.0 ± 0.0
Phe
2.372PheAla: 2.372 ± 0.422
0.222PheCys: 0.222 ± 0.115
2.743PheAsp: 2.743 ± 0.455
1.705PheGlu: 1.705 ± 0.317
1.26PhePhe: 1.26 ± 0.511
3.41PheGly: 3.41 ± 0.723
0.593PheHis: 0.593 ± 0.252
0.741PheIle: 0.741 ± 0.225
1.483PheLys: 1.483 ± 0.337
1.631PheLeu: 1.631 ± 0.256
0.815PheMet: 0.815 ± 0.232
0.964PheAsn: 0.964 ± 0.258
0.741PhePro: 0.741 ± 0.285
1.483PheGln: 1.483 ± 0.329
2.15PheArg: 2.15 ± 0.299
2.372PheSer: 2.372 ± 0.542
2.15PheThr: 2.15 ± 0.55
2.224PheVal: 2.224 ± 0.454
0.074PheTrp: 0.074 ± 0.076
0.815PheTyr: 0.815 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
7.858GlyAla: 7.858 ± 1.022
0.815GlyCys: 0.815 ± 0.277
4.893GlyAsp: 4.893 ± 0.651
5.337GlyGlu: 5.337 ± 0.744
3.113GlyPhe: 3.113 ± 0.635
6.004GlyGly: 6.004 ± 0.726
2.001GlyHis: 2.001 ± 0.49
4.077GlyIle: 4.077 ± 0.679
3.855GlyLys: 3.855 ± 0.45
7.709GlyLeu: 7.709 ± 0.959
2.076GlyMet: 2.076 ± 0.322
4.151GlyAsn: 4.151 ± 0.588
4.299GlyPro: 4.299 ± 0.587
3.855GlyGln: 3.855 ± 0.513
3.929GlyArg: 3.929 ± 0.606
6.301GlySer: 6.301 ± 0.87
5.782GlyThr: 5.782 ± 0.965
7.042GlyVal: 7.042 ± 0.742
2.001GlyTrp: 2.001 ± 0.481
2.743GlyTyr: 2.743 ± 0.55
0.0GlyXaa: 0.0 ± 0.0
His
1.631HisAla: 1.631 ± 0.336
0.148HisCys: 0.148 ± 0.106
1.483HisAsp: 1.483 ± 0.317
0.89HisGlu: 0.89 ± 0.3
0.519HisPhe: 0.519 ± 0.207
1.408HisGly: 1.408 ± 0.281
0.519HisHis: 0.519 ± 0.16
1.408HisIle: 1.408 ± 0.314
1.112HisLys: 1.112 ± 0.263
1.779HisLeu: 1.779 ± 0.413
0.371HisMet: 0.371 ± 0.166
0.667HisAsn: 0.667 ± 0.245
0.667HisPro: 0.667 ± 0.194
0.445HisGln: 0.445 ± 0.161
0.667HisArg: 0.667 ± 0.248
1.26HisSer: 1.26 ± 0.246
1.186HisThr: 1.186 ± 0.311
1.334HisVal: 1.334 ± 0.338
0.593HisTrp: 0.593 ± 0.232
0.667HisTyr: 0.667 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
4.374IleAla: 4.374 ± 0.629
0.371IleCys: 0.371 ± 0.173
3.188IleAsp: 3.188 ± 0.537
3.484IleGlu: 3.484 ± 0.536
1.186IlePhe: 1.186 ± 0.385
3.706IleGly: 3.706 ± 0.634
0.741IleHis: 0.741 ± 0.235
2.372IleIle: 2.372 ± 0.625
2.372IleLys: 2.372 ± 0.41
3.262IleLeu: 3.262 ± 0.469
0.815IleMet: 0.815 ± 0.333
2.224IleAsn: 2.224 ± 0.397
2.372IlePro: 2.372 ± 0.416
1.705IleGln: 1.705 ± 0.302
3.484IleArg: 3.484 ± 0.471
3.484IleSer: 3.484 ± 0.575
3.039IleThr: 3.039 ± 0.408
2.817IleVal: 2.817 ± 0.639
0.519IleTrp: 0.519 ± 0.209
0.815IleTyr: 0.815 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
6.227LysAla: 6.227 ± 0.841
0.667LysCys: 0.667 ± 0.248
3.113LysAsp: 3.113 ± 0.554
2.15LysGlu: 2.15 ± 0.343
1.705LysPhe: 1.705 ± 0.411
3.632LysGly: 3.632 ± 0.527
1.26LysHis: 1.26 ± 0.382
3.188LysIle: 3.188 ± 0.524
2.15LysLys: 2.15 ± 0.428
4.151LysLeu: 4.151 ± 0.578
2.001LysMet: 2.001 ± 0.393
0.815LysAsn: 0.815 ± 0.271
2.669LysPro: 2.669 ± 0.459
1.408LysGln: 1.408 ± 0.303
2.743LysArg: 2.743 ± 0.501
2.372LysSer: 2.372 ± 0.472
3.262LysThr: 3.262 ± 0.571
3.262LysVal: 3.262 ± 0.467
0.371LysTrp: 0.371 ± 0.171
1.186LysTyr: 1.186 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
9.118LeuAla: 9.118 ± 0.855
0.667LeuCys: 0.667 ± 0.232
5.782LeuAsp: 5.782 ± 0.628
4.448LeuGlu: 4.448 ± 0.583
2.298LeuPhe: 2.298 ± 0.419
6.968LeuGly: 6.968 ± 0.869
1.927LeuHis: 1.927 ± 0.431
4.374LeuIle: 4.374 ± 0.578
3.188LeuLys: 3.188 ± 0.593
6.746LeuLeu: 6.746 ± 0.84
1.557LeuMet: 1.557 ± 0.366
2.595LeuAsn: 2.595 ± 0.485
4.67LeuPro: 4.67 ± 0.761
3.558LeuGln: 3.558 ± 0.464
5.189LeuArg: 5.189 ± 0.616
5.337LeuSer: 5.337 ± 0.567
4.744LeuThr: 4.744 ± 0.602
7.116LeuVal: 7.116 ± 0.899
1.927LeuTrp: 1.927 ± 0.381
2.298LeuTyr: 2.298 ± 0.344
0.0LeuXaa: 0.0 ± 0.0
Met
2.446MetAla: 2.446 ± 0.438
0.074MetCys: 0.074 ± 0.071
1.334MetAsp: 1.334 ± 0.373
1.186MetGlu: 1.186 ± 0.35
0.371MetPhe: 0.371 ± 0.157
1.631MetGly: 1.631 ± 0.485
0.445MetHis: 0.445 ± 0.216
1.557MetIle: 1.557 ± 0.433
1.038MetLys: 1.038 ± 0.296
1.631MetLeu: 1.631 ± 0.315
0.667MetMet: 0.667 ± 0.214
1.038MetAsn: 1.038 ± 0.275
1.927MetPro: 1.927 ± 0.385
0.741MetGln: 0.741 ± 0.292
0.815MetArg: 0.815 ± 0.282
1.631MetSer: 1.631 ± 0.339
1.927MetThr: 1.927 ± 0.381
2.224MetVal: 2.224 ± 0.385
0.371MetTrp: 0.371 ± 0.141
0.371MetTyr: 0.371 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.262AsnAla: 3.262 ± 0.431
0.445AsnCys: 0.445 ± 0.219
2.076AsnAsp: 2.076 ± 0.425
2.001AsnGlu: 2.001 ± 0.345
0.964AsnPhe: 0.964 ± 0.281
4.448AsnGly: 4.448 ± 0.714
0.297AsnHis: 0.297 ± 0.188
1.26AsnIle: 1.26 ± 0.278
1.631AsnLys: 1.631 ± 0.338
3.262AsnLeu: 3.262 ± 0.563
0.89AsnMet: 0.89 ± 0.281
1.186AsnAsn: 1.186 ± 0.363
2.446AsnPro: 2.446 ± 0.475
1.186AsnGln: 1.186 ± 0.332
1.705AsnArg: 1.705 ± 0.374
1.631AsnSer: 1.631 ± 0.264
2.446AsnThr: 2.446 ± 0.446
2.817AsnVal: 2.817 ± 0.448
0.593AsnTrp: 0.593 ± 0.2
0.815AsnTyr: 0.815 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
5.708ProAla: 5.708 ± 0.76
0.074ProCys: 0.074 ± 0.083
3.039ProAsp: 3.039 ± 0.518
3.632ProGlu: 3.632 ± 0.566
1.112ProPhe: 1.112 ± 0.296
5.782ProGly: 5.782 ± 0.792
0.593ProHis: 0.593 ± 0.217
2.298ProIle: 2.298 ± 0.508
2.965ProLys: 2.965 ± 0.508
3.113ProLeu: 3.113 ± 0.406
0.89ProMet: 0.89 ± 0.221
1.483ProAsn: 1.483 ± 0.374
1.927ProPro: 1.927 ± 0.471
1.112ProGln: 1.112 ± 0.263
2.669ProArg: 2.669 ± 0.53
3.855ProSer: 3.855 ± 0.511
3.336ProThr: 3.336 ± 0.614
4.448ProVal: 4.448 ± 0.551
1.334ProTrp: 1.334 ± 0.342
0.815ProTyr: 0.815 ± 0.269
0.0ProXaa: 0.0 ± 0.0
Gln
3.929GlnAla: 3.929 ± 0.503
0.148GlnCys: 0.148 ± 0.105
1.927GlnAsp: 1.927 ± 0.396
2.224GlnGlu: 2.224 ± 0.408
1.334GlnPhe: 1.334 ± 0.309
3.336GlnGly: 3.336 ± 0.49
0.815GlnHis: 0.815 ± 0.278
2.076GlnIle: 2.076 ± 0.457
2.52GlnLys: 2.52 ± 0.459
4.893GlnLeu: 4.893 ± 0.714
0.89GlnMet: 0.89 ± 0.278
1.557GlnAsn: 1.557 ± 0.343
2.15GlnPro: 2.15 ± 0.429
1.779GlnGln: 1.779 ± 0.309
2.076GlnArg: 2.076 ± 0.419
1.705GlnSer: 1.705 ± 0.426
2.446GlnThr: 2.446 ± 0.349
3.188GlnVal: 3.188 ± 0.52
1.038GlnTrp: 1.038 ± 0.289
0.741GlnTyr: 0.741 ± 0.278
0.0GlnXaa: 0.0 ± 0.0
Arg
5.115ArgAla: 5.115 ± 0.596
0.593ArgCys: 0.593 ± 0.249
3.632ArgAsp: 3.632 ± 0.58
4.374ArgGlu: 4.374 ± 0.756
1.557ArgPhe: 1.557 ± 0.379
4.003ArgGly: 4.003 ± 0.502
1.186ArgHis: 1.186 ± 0.335
2.669ArgIle: 2.669 ± 0.367
3.781ArgLys: 3.781 ± 0.638
6.004ArgLeu: 6.004 ± 0.774
1.483ArgMet: 1.483 ± 0.317
2.372ArgAsn: 2.372 ± 0.389
3.262ArgPro: 3.262 ± 0.553
1.853ArgGln: 1.853 ± 0.374
4.596ArgArg: 4.596 ± 0.698
3.558ArgSer: 3.558 ± 0.568
2.743ArgThr: 2.743 ± 0.369
4.299ArgVal: 4.299 ± 0.751
0.964ArgTrp: 0.964 ± 0.334
1.26ArgTyr: 1.26 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
4.522SerAla: 4.522 ± 0.537
0.297SerCys: 0.297 ± 0.148
3.188SerAsp: 3.188 ± 0.515
4.744SerGlu: 4.744 ± 0.516
2.224SerPhe: 2.224 ± 0.356
7.709SerGly: 7.709 ± 0.99
1.112SerHis: 1.112 ± 0.28
3.558SerIle: 3.558 ± 0.635
3.039SerLys: 3.039 ± 0.471
5.041SerLeu: 5.041 ± 0.787
1.779SerMet: 1.779 ± 0.404
2.076SerAsn: 2.076 ± 0.45
3.188SerPro: 3.188 ± 0.626
2.372SerGln: 2.372 ± 0.521
3.188SerArg: 3.188 ± 0.534
3.262SerSer: 3.262 ± 0.575
3.855SerThr: 3.855 ± 0.581
4.225SerVal: 4.225 ± 0.65
1.038SerTrp: 1.038 ± 0.433
1.927SerTyr: 1.927 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
7.339ThrAla: 7.339 ± 0.859
0.815ThrCys: 0.815 ± 0.269
2.891ThrAsp: 2.891 ± 0.559
3.558ThrGlu: 3.558 ± 0.614
1.853ThrPhe: 1.853 ± 0.37
4.893ThrGly: 4.893 ± 1.275
0.815ThrHis: 0.815 ± 0.305
1.927ThrIle: 1.927 ± 0.377
3.336ThrLys: 3.336 ± 0.496
5.782ThrLeu: 5.782 ± 0.575
1.186ThrMet: 1.186 ± 0.299
2.446ThrAsn: 2.446 ± 0.501
3.336ThrPro: 3.336 ± 0.459
2.446ThrGln: 2.446 ± 0.444
3.41ThrArg: 3.41 ± 0.618
4.077ThrSer: 4.077 ± 0.484
4.596ThrThr: 4.596 ± 0.794
4.818ThrVal: 4.818 ± 0.704
1.038ThrTrp: 1.038 ± 0.323
1.705ThrTyr: 1.705 ± 0.348
0.0ThrXaa: 0.0 ± 0.0
Val
7.339ValAla: 7.339 ± 0.77
0.297ValCys: 0.297 ± 0.152
3.188ValAsp: 3.188 ± 0.519
5.115ValGlu: 5.115 ± 0.506
2.15ValPhe: 2.15 ± 0.467
5.708ValGly: 5.708 ± 0.592
2.001ValHis: 2.001 ± 0.418
3.039ValIle: 3.039 ± 0.523
2.965ValLys: 2.965 ± 0.461
4.744ValLeu: 4.744 ± 0.713
2.446ValMet: 2.446 ± 0.432
2.595ValAsn: 2.595 ± 0.431
3.781ValPro: 3.781 ± 0.562
3.855ValGln: 3.855 ± 0.581
4.596ValArg: 4.596 ± 0.528
5.708ValSer: 5.708 ± 0.799
3.929ValThr: 3.929 ± 0.535
5.411ValVal: 5.411 ± 0.702
1.779ValTrp: 1.779 ± 0.324
1.779ValTyr: 1.779 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
2.15TrpAla: 2.15 ± 0.411
0.074TrpCys: 0.074 ± 0.076
1.483TrpAsp: 1.483 ± 0.376
1.112TrpGlu: 1.112 ± 0.287
0.593TrpPhe: 0.593 ± 0.212
1.779TrpGly: 1.779 ± 0.409
0.297TrpHis: 0.297 ± 0.145
0.964TrpIle: 0.964 ± 0.251
0.815TrpLys: 0.815 ± 0.234
1.334TrpLeu: 1.334 ± 0.246
0.593TrpMet: 0.593 ± 0.205
0.667TrpAsn: 0.667 ± 0.227
0.964TrpPro: 0.964 ± 0.339
0.667TrpGln: 0.667 ± 0.224
0.964TrpArg: 0.964 ± 0.25
1.483TrpSer: 1.483 ± 0.421
1.557TrpThr: 1.557 ± 0.42
1.927TrpVal: 1.927 ± 0.357
0.593TrpTrp: 0.593 ± 0.186
0.297TrpTyr: 0.297 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.001TyrAla: 2.001 ± 0.409
0.222TyrCys: 0.222 ± 0.12
1.557TyrAsp: 1.557 ± 0.295
1.705TyrGlu: 1.705 ± 0.354
0.964TyrPhe: 0.964 ± 0.205
2.372TyrGly: 2.372 ± 0.412
0.667TyrHis: 0.667 ± 0.226
1.112TyrIle: 1.112 ± 0.253
1.557TyrLys: 1.557 ± 0.349
2.15TyrLeu: 2.15 ± 0.351
0.222TyrMet: 0.222 ± 0.145
0.519TyrAsn: 0.519 ± 0.191
1.26TyrPro: 1.26 ± 0.3
0.89TyrGln: 0.89 ± 0.34
2.372TyrArg: 2.372 ± 0.407
1.186TyrSer: 1.186 ± 0.34
1.705TyrThr: 1.705 ± 0.471
1.631TyrVal: 1.631 ± 0.324
0.593TyrTrp: 0.593 ± 0.201
0.667TyrTyr: 0.667 ± 0.27
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13491 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski