Streptococcus satellite phage Javan603
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZWH4|A0A4D5ZWH4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan603 OX=2558772 GN=JavanS603_0013 PE=4 SV=1
MM1 pKa = 7.28 SVIGNFLKK9 pKa = 9.79 EE10 pKa = 3.94 TRR12 pKa = 11.84 KK13 pKa = 10.6 DD14 pKa = 3.43 KK15 pKa = 10.81 NIKK18 pKa = 9.89 VVTLAKK24 pKa = 10.06 QVGISQPYY32 pKa = 8.74 ISNIEE37 pKa = 3.82 NGKK40 pKa = 10.26 RR41 pKa = 11.84 GVTKK45 pKa = 10.3 EE46 pKa = 4.13 LFFKK50 pKa = 10.45 IIYY53 pKa = 10.05 AIAEE57 pKa = 4.24 LSPITRR63 pKa = 11.84 NVYY66 pKa = 10.58 DD67 pKa = 3.8 EE68 pKa = 5.41 LGLSEE73 pKa = 4.34 EE74 pKa = 4.4 KK75 pKa = 10.59 KK76 pKa = 10.88 EE77 pKa = 4.74 EE78 pKa = 3.85 IDD80 pKa = 3.96 IDD82 pKa = 3.87 DD83 pKa = 4.04 TLGEE87 pKa = 4.32 FWDD90 pKa = 4.73 EE91 pKa = 4.25 YY92 pKa = 11.37 YY93 pKa = 11.24 DD94 pKa = 6.22 DD95 pKa = 5.14 IKK97 pKa = 11.26 QDD99 pKa = 3.72 LNSYY103 pKa = 9.68 MDD105 pKa = 5.28 DD106 pKa = 3.72 DD107 pKa = 4.56 EE108 pKa = 6.5 KK109 pKa = 11.27 PILTLEE115 pKa = 4.5 DD116 pKa = 3.57 FLDD119 pKa = 4.6 YY120 pKa = 10.29 VQSWSFEE127 pKa = 4.09 DD128 pKa = 3.48 MLTISGYY135 pKa = 11.1 SDD137 pKa = 3.76 FLDD140 pKa = 3.39 SKK142 pKa = 11.17 YY143 pKa = 11.13 GVDD146 pKa = 3.33 NYY148 pKa = 10.35 MGYY151 pKa = 11.24 GDD153 pKa = 3.94 YY154 pKa = 10.6 QDD156 pKa = 3.79 YY157 pKa = 11.33 SSYY160 pKa = 11.22 TNPEE164 pKa = 3.93 YY165 pKa = 10.69 VQEE168 pKa = 4.05 LVFDD172 pKa = 3.51 YY173 pKa = 9.62 WYY175 pKa = 11.06 QSILSDD181 pKa = 3.57 FLEE184 pKa = 4.53 LFEE187 pKa = 7.0 IDD189 pKa = 4.45 LSPIEE194 pKa = 4.44 TKK196 pKa = 10.31 IEE198 pKa = 4.24 TNWALLNEE206 pKa = 4.22 LDD208 pKa = 3.72 KK209 pKa = 11.67 QEE211 pKa = 4.12 FEE213 pKa = 4.38 IYY215 pKa = 9.34 STVKK219 pKa = 10.27 SLRR222 pKa = 11.84 SKK224 pKa = 9.71 SGYY227 pKa = 7.37 FARR230 pKa = 11.84 YY231 pKa = 8.59 HH232 pKa = 5.98 LQDD235 pKa = 3.43 EE236 pKa = 4.66 NKK238 pKa = 10.31 EE239 pKa = 4.1 KK240 pKa = 10.94 LVDD243 pKa = 4.48 LNILYY248 pKa = 9.96 DD249 pKa = 3.44 QEE251 pKa = 4.79 SIYY254 pKa = 11.37 NVTFDD259 pKa = 4.24 GKK261 pKa = 9.65 PLSEE265 pKa = 4.21 EE266 pKa = 4.68 DD267 pKa = 4.1 IIALRR272 pKa = 11.84 NTLNGIRR279 pKa = 11.84 YY280 pKa = 9.43 SKK282 pKa = 11.19
Molecular weight: 33.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.176
IPC2_protein 4.177
IPC_protein 4.151
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.986
Grimsley 3.872
Solomon 4.113
Lehninger 4.062
Nozaki 4.215
DTASelect 4.418
Thurlkill 3.986
EMBOSS 4.024
Sillero 4.266
Patrickios 3.261
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.172
Protein with the highest isoelectric point:
>tr|A0A4D5ZRS1|A0A4D5ZRS1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan603 OX=2558772 GN=JavanS603_0012 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANIYY20 pKa = 10.62 LGVDD24 pKa = 3.95 VITGKK29 pKa = 10.74 KK30 pKa = 7.55 VTTKK34 pKa = 9.32 VTARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.48 EE42 pKa = 4.24 LKK44 pKa = 9.67 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.72 KK63 pKa = 9.99 EE64 pKa = 4.21 VAIEE68 pKa = 4.25 TYY70 pKa = 10.63 EE71 pKa = 3.95 EE72 pKa = 4.45 LAISWWDD79 pKa = 3.38 SYY81 pKa = 11.84 KK82 pKa = 10.19 NTVKK86 pKa = 10.95 ANTKK90 pKa = 9.64 RR91 pKa = 11.84 AQKK94 pKa = 10.81 GLLNNHH100 pKa = 5.72 VLPLFGEE107 pKa = 4.67 FKK109 pKa = 10.56 LDD111 pKa = 3.59 KK112 pKa = 10.06 LTTPLIQSTMNKK124 pKa = 9.46 LANSTNTGEE133 pKa = 4.11 AGAYY137 pKa = 8.96 LHH139 pKa = 6.56 YY140 pKa = 11.05 DD141 pKa = 4.09 KK142 pKa = 11.05 IHH144 pKa = 6.58 ALNKK148 pKa = 10.18 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVVLQVIPTNPANNVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.1 DD177 pKa = 3.22 KK178 pKa = 10.56 KK179 pKa = 10.89 AKK181 pKa = 9.81 VKK183 pKa = 10.6 HH184 pKa = 5.76 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.53 LDD198 pKa = 4.07 SLDD201 pKa = 3.34 NSKK204 pKa = 11.04 YY205 pKa = 10.38 KK206 pKa = 10.64 NYY208 pKa = 10.65 YY209 pKa = 10.47 DD210 pKa = 3.03 ITLYY214 pKa = 10.98 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.57 ITKK246 pKa = 9.23 TLNYY250 pKa = 9.33 EE251 pKa = 4.08 MKK253 pKa = 10.78 LNSPKK258 pKa = 10.55 SKK260 pKa = 10.22 SGYY263 pKa = 9.62 RR264 pKa = 11.84 DD265 pKa = 2.83 IDD267 pKa = 3.15 IDD269 pKa = 3.87 KK270 pKa = 9.04 QTVTMMKK277 pKa = 9.91 RR278 pKa = 11.84 YY279 pKa = 7.4 QHH281 pKa = 6.34 KK282 pKa = 8.59 QTQEE286 pKa = 3.08 AWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.07 TVVFSDD300 pKa = 6.21 FIHH303 pKa = 7.08 EE304 pKa = 4.36 YY305 pKa = 9.57 PNSRR309 pKa = 11.84 TLQSRR314 pKa = 11.84 LGTHH318 pKa = 6.83 FKK320 pKa = 10.69 RR321 pKa = 11.84 AGVTNIGFHH330 pKa = 6.07 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.88 RR353 pKa = 11.84 LGHH356 pKa = 6.17 SKK358 pKa = 11.08 LSMTMDD364 pKa = 3.56 IYY366 pKa = 11.68 SHH368 pKa = 6.98 LSKK371 pKa = 11.0 EE372 pKa = 4.25 NAKK375 pKa = 10.37 KK376 pKa = 10.27 AVSFYY381 pKa = 10.95 EE382 pKa = 4.17 VALKK386 pKa = 10.72 SII388 pKa = 4.28
Molecular weight: 44.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.012
IPC2_protein 9.194
IPC_protein 9.063
Toseland 9.882
ProMoST 9.545
Dawson 10.087
Bjellqvist 9.736
Wikipedia 10.248
Rodwell 10.628
Grimsley 10.16
Solomon 10.101
Lehninger 10.072
Nozaki 9.853
DTASelect 9.721
Thurlkill 9.94
EMBOSS 10.292
Sillero 9.999
Patrickios 10.043
IPC_peptide 10.101
IPC2_peptide 8.156
IPC2.peptide.svr19 8.132
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2284
61
500
163.1
18.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.166 ± 0.5
0.482 ± 0.124
6.217 ± 0.641
7.399 ± 0.804
3.984 ± 0.389
5.342 ± 0.387
1.532 ± 0.449
6.655 ± 0.59
8.932 ± 0.732
9.632 ± 0.578
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.189 ± 0.321
7.005 ± 0.483
3.284 ± 0.81
3.24 ± 0.347
4.028 ± 0.348
6.042 ± 0.729
6.743 ± 0.725
5.911 ± 0.345
1.051 ± 0.229
5.166 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here