Succiniclasticum ruminis DSM 9236

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Succiniclasticum; Succiniclasticum ruminis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2DTY6|A0A1I2DTY6_9FIRM Probable membrane transporter protein OS=Succiniclasticum ruminis DSM 9236 OX=1123323 GN=SAMN05216245_1243 PE=3 SV=1
MM1 pKa = 7.86TDD3 pKa = 3.52NDD5 pKa = 4.47CLSDD9 pKa = 4.61EE10 pKa = 4.59IDD12 pKa = 3.57GSINAAYY19 pKa = 9.11WDD21 pKa = 3.83GLITIEE27 pKa = 3.93VAEE30 pKa = 4.13YY31 pKa = 10.86LRR33 pKa = 11.84GKK35 pKa = 10.17NFGIEE40 pKa = 3.62DD41 pKa = 4.49PYY43 pKa = 11.2RR44 pKa = 11.84IFIDD48 pKa = 4.7DD49 pKa = 4.41LFDD52 pKa = 3.28MM53 pKa = 5.58

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2C3Y0|A0A1I2C3Y0_9FIRM Peptidoglycan/xylan/chitin deacetylase PgdA/CDA1 family OS=Succiniclasticum ruminis DSM 9236 OX=1123323 GN=SAMN05216245_1118 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.16SKK4 pKa = 10.37RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84MRR10 pKa = 11.84KK11 pKa = 7.44MASEE15 pKa = 4.18RR16 pKa = 11.84VLMDD20 pKa = 3.69GYY22 pKa = 10.15GQEE25 pKa = 3.88YY26 pKa = 10.16HH27 pKa = 6.92IVEE30 pKa = 3.96KK31 pKa = 10.57RR32 pKa = 11.84LPGRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.5SVYY41 pKa = 7.91DD42 pKa = 3.4TYY44 pKa = 11.49VVRR47 pKa = 11.84QKK49 pKa = 10.2RR50 pKa = 11.84TGRR53 pKa = 11.84DD54 pKa = 3.4YY55 pKa = 11.28LVEE58 pKa = 5.78IMLQWLIRR66 pKa = 11.84LVASMLPVGDD76 pKa = 3.88WNLSEE81 pKa = 4.14WCC83 pKa = 4.21

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2273

0

2273

742859

30

5372

326.8

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.1 ± 0.075

1.37 ± 0.028

5.458 ± 0.04

6.819 ± 0.065

3.974 ± 0.046

7.789 ± 0.076

1.828 ± 0.028

6.279 ± 0.045

6.712 ± 0.057

9.133 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.948 ± 0.037

4.201 ± 0.058

3.833 ± 0.047

3.141 ± 0.034

4.852 ± 0.06

5.265 ± 0.058

5.506 ± 0.076

7.245 ± 0.047

1.008 ± 0.02

3.538 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski