Halovibrio salipaludis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halovibrio

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2EV44|A0A2A2EV44_9GAMM Uncharacterized protein OS=Halovibrio salipaludis OX=2032626 GN=CK501_15415 PE=4 SV=1
MM1 pKa = 7.28QRR3 pKa = 11.84QQQGFTLIEE12 pKa = 3.93LVIVIVILGILSAFALPRR30 pKa = 11.84FADD33 pKa = 3.47LGGDD37 pKa = 3.28ARR39 pKa = 11.84EE40 pKa = 4.14ATRR43 pKa = 11.84DD44 pKa = 4.02GIVGAMKK51 pKa = 10.03SASSIAHH58 pKa = 5.54SKK60 pKa = 10.71CLTSSDD66 pKa = 4.33CEE68 pKa = 3.94ASADD72 pKa = 3.63EE73 pKa = 4.53STVSLEE79 pKa = 3.91NQTIQMVYY87 pKa = 10.47GYY89 pKa = 9.08PDD91 pKa = 4.54SIADD95 pKa = 4.55GIGQAAQIDD104 pKa = 4.75APTASAGTSGGTITYY119 pKa = 10.26NVDD122 pKa = 3.79DD123 pKa = 4.92SADD126 pKa = 3.65CQITYY131 pKa = 8.71TAASEE136 pKa = 4.08DD137 pKa = 4.13TDD139 pKa = 3.6TGEE142 pKa = 3.86ISSPSVATSGDD153 pKa = 3.98CNN155 pKa = 3.66

Molecular weight: 15.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2ET78|A0A2A2ET78_9GAMM Uncharacterized protein OS=Halovibrio salipaludis OX=2032626 GN=CK501_16275 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATQNGRR28 pKa = 11.84KK29 pKa = 9.4VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3225

0

3225

1065101

26

3510

330.3

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.911 ± 0.049

0.876 ± 0.013

5.987 ± 0.038

7.276 ± 0.045

3.473 ± 0.027

8.29 ± 0.042

2.364 ± 0.024

4.71 ± 0.029

2.635 ± 0.03

10.499 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.022

3.021 ± 0.025

4.881 ± 0.03

4.136 ± 0.031

7.421 ± 0.05

5.684 ± 0.033

5.237 ± 0.036

7.072 ± 0.04

1.446 ± 0.019

2.49 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski