Ereboglobus luteus
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8E583|A0A2U8E583_9BACT Hydrophobe/amphiphile efflux-1 family RND transporter OS=Ereboglobus luteus OX=1796921 GN=CKA38_12705 PE=3 SV=1
MM1 pKa = 7.2 FRR3 pKa = 11.84 HH4 pKa = 6.13 PCGAGEE10 pKa = 4.26 AGFEE14 pKa = 4.08 IGDD17 pKa = 3.82 GGGDD21 pKa = 3.05 AGLPGFEE28 pKa = 4.55 SGEE31 pKa = 4.08 GDD33 pKa = 3.21 FGGDD37 pKa = 2.74 VALEE41 pKa = 4.03 VDD43 pKa = 3.86 GGAVVEE49 pKa = 4.44 GALDD53 pKa = 3.74 GGADD57 pKa = 3.2 IGEE60 pKa = 4.36 IPAQAGEE67 pKa = 3.91 ALAYY71 pKa = 10.22 GSGFRR76 pKa = 11.84 GVGCGHH82 pKa = 6.9 GCSS85 pKa = 3.74
Molecular weight: 8.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.516
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A2U8DZY0|A0A2U8DZY0_9BACT MR_MLE_C domain-containing protein OS=Ereboglobus luteus OX=1796921 GN=CKA38_01700 PE=4 SV=1
MM1 pKa = 7.15 QPTFRR6 pKa = 11.84 PHH8 pKa = 5.63 RR9 pKa = 11.84 KK10 pKa = 8.98 KK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 QIGFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 6.5 STKK25 pKa = 9.83 GGRR28 pKa = 11.84 KK29 pKa = 8.7 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 VGRR39 pKa = 11.84 KK40 pKa = 8.97 RR41 pKa = 11.84 LTVVV45 pKa = 3.08
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2881
0
2881
1204893
31
6432
418.2
45.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.316 ± 0.055
0.943 ± 0.019
5.354 ± 0.028
5.072 ± 0.06
3.886 ± 0.027
8.499 ± 0.115
2.096 ± 0.021
5.67 ± 0.039
4.345 ± 0.056
9.136 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.052 ± 0.026
4.04 ± 0.064
4.935 ± 0.067
2.945 ± 0.026
6.182 ± 0.073
6.039 ± 0.058
6.667 ± 0.1
6.595 ± 0.04
1.451 ± 0.024
2.777 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here