Massilia sp. NR 4-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1JZR5|A0A0K1JZR5_9BURK Twitching motility protein PilT OS=Massilia sp. NR 4-1 OX=1678028 GN=ACZ75_14395 PE=3 SV=1
MM1 pKa = 7.22SAVAEE6 pKa = 4.34AQDD9 pKa = 4.6VIPSPIIFTDD19 pKa = 3.69AAAEE23 pKa = 4.14KK24 pKa = 9.63VAQLIEE30 pKa = 4.38EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.58NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.78NPGASSTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1JVA0|A0A0K1JVA0_9BURK Cytochrome C biogenesis protein OS=Massilia sp. NR 4-1 OX=1678028 GN=ACZ75_05530 PE=3 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84GGGFPGCCINIARR15 pKa = 11.84MQLEE19 pKa = 5.03LFPFDD24 pKa = 3.31QLSTVEE30 pKa = 4.16LRR32 pKa = 11.84MAFSLIRR39 pKa = 11.84NSYY42 pKa = 6.08WHH44 pKa = 6.47IRR46 pKa = 11.84NLRR49 pKa = 11.84GGRR52 pKa = 11.84FGQGRR57 pKa = 11.84ARR59 pKa = 11.84SHH61 pKa = 5.26YY62 pKa = 10.05RR63 pKa = 11.84AVARR67 pKa = 11.84QKK69 pKa = 10.88KK70 pKa = 8.15RR71 pKa = 11.84LLMAGVSKK79 pKa = 11.12RR80 pKa = 11.84EE81 pKa = 3.76ILDD84 pKa = 3.66FLACCRR90 pKa = 11.84LQCAAHH96 pKa = 6.08KK97 pKa = 10.55QPFKK101 pKa = 10.22PCPYY105 pKa = 8.93CTNN108 pKa = 3.58

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5022

0

5022

1773294

29

9089

353.1

38.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.977 ± 0.071

0.863 ± 0.012

5.118 ± 0.029

5.411 ± 0.029

3.511 ± 0.021

8.18 ± 0.031

2.141 ± 0.019

4.522 ± 0.029

3.644 ± 0.039

10.988 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.019

3.066 ± 0.028

4.902 ± 0.029

4.38 ± 0.026

6.441 ± 0.032

5.789 ± 0.03

4.8 ± 0.034

6.904 ± 0.028

1.316 ± 0.015

2.588 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski